First trimester trophoblasts forming endothelial-like tubes in vitro emulate a ‘blood vessel development’ gene expression profile
Publication date: Available online 21 May 2016 Source:Gene Expression Patterns Author(s): Amanda R. Highet, Sam Buckberry, Benjamin T. Mayne, Sultana M. Khoda, Tina Bianco-Miotto, Claire T. Roberts Extravillous cytotrophoblasts isolated from first trimester placenta, and immortalised cell lines derived from them, have the intrinsic ability to form endothelial-like tubes when cultured on Matrigel™ extracellular matrix. This in vitro tube formation may model placental angiogenesis and/or endovascular differentiation by trophoblasts. To interpret the relevance of this phenomenon to placental development, we ...
Source: Gene Expression Patterns - May 21, 2016 Category: Genetics & Stem Cells Source Type: research
Expression of Prolyl hydroxylases 2 and 3 in chick embryos
Publication date: Available online 8 April 2016 Source:Gene Expression Patterns Author(s): Jake Barber, Nobue Itasaki Hypoxic cellular response is crucial for normal development as well as in pathological conditions in order to tolerate low oxygen. The response is mediated by Hypoxia Inducible Factors (HIFs), where the α-subunit of HIF is stabilised and able to function only in low oxygen. Prolyl hydroxyrases (PHDs) are oxygen dependent dioxygenase enzymes that hydroxyrate HIF-α leading to HIF degradation. Thus PHDs function as an oxygen sensor for the function of HIFs. Here we describe the mRNA expression patter...
Source: Gene Expression Patterns - April 7, 2016 Category: Genetics & Stem Cells Source Type: research
New insights into the role of the Brdt protein in the regulation of development and spermatogenesis in the mouse
In this study, we characterized mice of several age groups who lacked the Brdt gene. The testes of Brdt mutant mice aged 8 weeks exhibited complete spermatocyte maturation arrest with a significantly increased number of apoptotic cells. The weights of the testes and accessory glands as well as the testosterone levels of the mutant mice were significantly lower compared to the normal mice. The mutant mice had delayed puberty, with normal levels of testosterone and accessory gland weights at the age of 14 and 28 weeks. The testes of the mutant mice at older ages also exhibited round spermatids. The presence of the BRDT prote...
Source: Gene Expression Patterns - March 18, 2016 Category: Genetics & Stem Cells Source Type: research
Regulation of twin of eyeless during Drosophila development
Publication date: Available online 11 March 2016 Source:Gene Expression Patterns Author(s): John Skottheim Honn, Linn Johansson, Åsa Rasmuson Lestander The Pax-6 protein is vital for eye development in all seeing animals, from sea urchins to humans. Either of the Pax6 genes in Drosophila (twin of eyeless and eyeless) can induce a gene cascade leading to formation of entire eyes when expressed ectopically. The twin of eyeless (toy) gene in Drosophila is expressed in the anterior region of the early fly embryo. At later stages it is expressed in the brain, ventral nerve cord and (eventually) the visual primordium ...
Source: Gene Expression Patterns - March 12, 2016 Category: Genetics & Stem Cells Source Type: research
Expression of forkhead box transcription factor genes Foxp1 and Foxp2 during jaw development
Publication date: Available online 9 March 2016 Source:Gene Expression Patterns Author(s): Jeffry M. Cesario, Asma A. Almaidhan, Juhee Jeong Development of the face is regulated by a large number of genes that are expressed in temporally and spatially specific patterns. While significant progress has been made on characterizing the genes that operate in the oral region of the face, those regulating development of the aboral (lateral) region remain largely unknown. Recently, we discovered that transcription factors LIM homeobox (LHX) 6 and LHX8, which are key regulators of oral development, repressed the expressio...
Source: Gene Expression Patterns - March 9, 2016 Category: Genetics & Stem Cells Source Type: research
The zebrafish fast myosin light chain mylpfa:H2B-GFP transgene is a useful tool for in vivo imaging of myocyte fusion in the vertebrate embryo
Conclusions Since we know so little about the molecules regulating vertebrate myocyte fusion, we propose that the mylpfa:H2B-GFP transgene will be a very useful reporter for conducting forward and reverse genetic screens to identify new components regulating vertebrate myocyte fusion. (Source: Gene Expression Patterns)
Source: Gene Expression Patterns - February 17, 2016 Category: Genetics & Stem Cells Source Type: research
The zebrafish fast myosin light chain mylpfa:H2B-GFP transgene is a useful tool for in vivo imaging of myocyte fusion in the vertebrate embryo
Conclusions Since we know so little about the molecules regulating vertebrate myocyte fusion, we propose that the mylpfa:H2B-GFP transgene will be a very useful reporter for conducting forward and reverse genetic screens to identify new components regulating vertebrate myocyte fusion. (Source: Gene Expression Patterns)
Source: Gene Expression Patterns - February 11, 2016 Category: Genetics & Stem Cells Source Type: research
Fat accumulation in differentiated brown adipocytes is linked with expression of Hox genes
Publication date: Available online 26 January 2016 Source:Gene Expression Patterns Author(s): Smita Singh, Yudhishthir S. Rajput, Amit K. Barui, Rajan Sharma, Tirtha K. Datta Homeobox (Hox) genes are involved in body plan of embryo along the anterior-posterior axis. Presence of several Hox genes in white adipose tissue (WAT) and brown adipose tissue (BAT) is indicative of involvement of Hox genes in adipogenesis. We propose that differentiation inducing agents viz. isobutyl-methyl-xanthine (IBMX), indomethacin, dexamethasone (DEX), triiodothyronine (T3) and insulin may regulate differentiation in brown adipo...
Source: Gene Expression Patterns - January 26, 2016 Category: Genetics & Stem Cells Source Type: research
Expression of FGFs during early mouse tongue development
Publication date: Available online 31 December 2015 Source:Gene Expression Patterns Author(s): Wen Du, Jan Prochazka, Michaela Prochazkova, Ophir D. Klein The fibroblast growth factors (FGFs) constitute one of the largest growth factor families, and several ligands and receptors in this family are known to play critical roles during tongue development. In order to provide a comprehensive foundation for research into the role of FGFs during the process of tongue formation, we measured the transcript levels by quantitative PCR and mapped the expression patterns by in situ hybridization of all 22 Fgfs during mouse...
Source: Gene Expression Patterns - January 11, 2016 Category: Genetics & Stem Cells Source Type: research
MicroRNA Expression Profiles in Response to Drought Stress in Sorghum bicolor
In this study, were report the expression profiling of 8 known abiotic stress deregulated miRNAs in 11 elite sorghum genotypes, under watered and drought conditions. Significant deregulation was observed with miR396, miR393, miR397-5p, miR166, miR167 and miR168. Among these, the expression levels of sbi-miR396 and sbi-miR398 were the highest in all the genotypes.The expression of sbi-miR396 was maximum in the grain Sorghum HSD3226 under well-watered conditions and the profile shifted towards HSD3221 under drought stress. Forage accessions, N98 and Atlas, showed an opposite behavior in expression patterns of miR397-5p in dr...
Source: Gene Expression Patterns - January 11, 2016 Category: Genetics & Stem Cells Source Type: research
Testicular receptor 2, Nr2c1, is associated with stem cells in the developing olfactory epithelium and other cranial sensory and skeletal structures
Publication date: Available online 19 December 2015 Source:Gene Expression Patterns Author(s): Jennifer L. Baker, Bernard Wood, Beverly A. Karpinski, Anthony-S. LaMantia, Thomas M. Maynard Comparative genomic analysis of the nuclear receptor family suggests that the testicular receptor 2, Nr2c1, undergoes positive selection in the human-chimpanzee clade based upon a significant increase in nonsynonymous compared to synonymous substitutions. Previous in situ analyses of Nr2c1 lacked the temporal range and spatial resolution necessary to characterize cellular expression of this gene from early to mid gestation,...
Source: Gene Expression Patterns - December 19, 2015 Category: Genetics & Stem Cells Source Type: research
Oocyte Maturation and Expression Pattern of Follicular Genes during In-Vitro Culture of Vitrified Mouse Pre-Antral Follicles
In conclusion, survival rate of cryotop vitrified pre-antral follicles reduced during culture period while oocytes maturation and follicular genes expression did not show any noticeable alteration. (Source: Gene Expression Patterns)
Source: Gene Expression Patterns - December 15, 2015 Category: Genetics & Stem Cells Source Type: research
Mapping of brain lipid binding protein (Blbp) in the brain of adult zebrafish, co-expression with aromatase B and links with proliferation
Publication date: Available online 30 November 2015 Source:Gene Expression Patterns Author(s): Nicolas Diotel, Colette Vaillant, Olivier Kah, Elisabeth Pellegrini Adult fish exhibit a strong neurogenic capacity due to the persistence of radial glial cells. In zebrafish, radial glial cells display well-established markers such as the estrogen-synthesizing enzyme (AroB) and the brain lipid binding protein (Blbp), which is known to strongly bind omega-3 polyunsaturated fatty acids such as docosahexaenoic acid (DHA). While Blpb is mainly described in the telencephalon of adult zebrafish, its expression in the remai...
Source: Gene Expression Patterns - November 30, 2015 Category: Genetics & Stem Cells Source Type: research
Expression pattern of bcar3, a downstream target of Gata2, and its binding partner, bcar1, during Xenopus development
Publication date: Available online 26 November 2015 Source:Gene Expression Patterns Author(s): Yangsook Song Green, Sunjong Kwon, Jan L. Christian Primitive hematopoiesis generates red blood cells that deliver oxygen to the developing embryo. Mesodermal cells commit to a primitive blood cell fate during gastrulation and, in order to do so the mesoderm must receive non-cell autonomous signals transmitted from other germ layers. In Xenopus, the transcription factor Gata2 functions in ectodermal cells to generate or transmit the non-cell autonomous signals. Here we have identified Breast Cancer Antiestrogen Resistan...
Source: Gene Expression Patterns - November 26, 2015 Category: Genetics & Stem Cells Source Type: research
The ontogeny of nanos homologue expression in the oligochaete annelid Tubifex tubifex
Publication date: Available online 11 November 2015 Source:Gene Expression Patterns Author(s): Ki-ichi Mohri, Ayaki Nakamoto, Takashi Shimizu We have cloned and characterized the expression of a nanos homologue (designated Ttu-nos) from the oligochaete annelid Tubifex tubifex. Ttu-nos mRNA is distributed broadly throughout the early cleavage stages. Ttu-nos is expressed in most if not all of the early blastomeres, in which Ttu-nos RNA associates with pole plasms. Ttu-nos transcripts are concentrated to 2d and 4d cells. Shortly after 2d111 (derived from 2d cell) divides into a bilateral pair of NOPQ proteloblasts,...
Source: Gene Expression Patterns - November 13, 2015 Category: Genetics & Stem Cells Source Type: research
