Transcript profiling of salt tolerant tobacco mutants generated via mutation breeding
Publication date: September 2018Source: Gene Expression Patterns, Volume 29Author(s): Özge Çelik, Aybüke Ekşioğlu, Enes Yağız AkdaşAbstractThe main aim of the study is to identify the genes differentially, predominantly or specifically expressed in salt tolerant tobacco mutants, improved from Akhisar 97 and İzmir Özbaş varieties via mutation breeding, with respect to unstressed control plants. Seven tobacco mutants which have different salt tolerance capacities were evaluated by Gene Fishing analysis. Under stress conditions differentially expressed 100 reproducible bands were identified (74 of up-regulated and ...
Source: Gene Expression Patterns - July 10, 2018 Category: Genetics & Stem Cells Source Type: research
Epigenetic modifications in the embryonic and induced pluripotent stem cells
Publication date: September 2018Source: Gene Expression Patterns, Volume 29Author(s): Rasoul Godini, Haider Yabr Lafta, Hossein FallahiAbstractEpigenetic modifications are involved in global reprogramming of the cell transcriptome. Therefore, synchronized major shifts in the expression of many genes could be achieved through epigenetic changes. The regulation of gene expression could be implemented by different epigenetic events including histone modifications, DNA methylation and chromatin remodelling. Interestingly, it has been documented that reprogramming of somatic cells to induced pluripotent stem (iPS) cells is also...
Source: Gene Expression Patterns - July 10, 2018 Category: Genetics & Stem Cells Source Type: research
Expression patterns of Yes-associated protein 1 in the developing mouse liver
Publication date: September 2018Source: Gene Expression Patterns, Volume 29Author(s): Wei Wei, Jeremy Lotto, Pamela A. HoodlessAbstractThe Hippo signaling pathway regulates many cellular processes, but has been specifically associated with control organ size and tumor growth. Yes-associated protein 1 (YAP1) is a transcriptional cofactor, in the Hippo pathway, that regulates gene expression when localized in the nucleus. Elevated expression of YAP1 in adult mouse liver leads to hepatomegaly and can cause hepatocellular carcinoma; while the loss of function studies reveal its importance in regulating cholangiocyte developmen...
Source: Gene Expression Patterns - July 10, 2018 Category: Genetics & Stem Cells Source Type: research
Enriched expression of the ciliopathy gene Ick in cell proliferating regions of adult mice
Publication date: September 2018Source: Gene Expression Patterns, Volume 29Author(s): Ryotaro Tsutsumi, Taro Chaya, Takahisa FurukawaAbstractCilia are essential for sensory and motile functions across species. In humans, ciliary dysfunction causes “ciliopathies”, which show severe developmental abnormalities in various tissues. Several missense mutations in intestinal cell kinase (ICK) gene lead to endocrine-cerebro-osteodysplasia syndrome or short rib-polydactyly syndrome, lethal recessive developmental ciliopathies. We and others previously reported that Ick-deficient mice exhibit neonatal lethality with developmenta...
Source: Gene Expression Patterns - July 10, 2018 Category: Genetics & Stem Cells Source Type: research
Identification of runt family genes involved in planarian regeneration and tissue homeostasis
In this study, the full-length cDNA sequences of two runt genes are first cloned from Dugesia japonica, and their roles are investigated by WISH and RNAi. The results show that: (1) the Djrunts are conserved during evolution; (2) the Djrunts mRNA are widely expressed in intact and regenerative worms, and their expression levels are up-regulated significantly on day 1 after amputation; (3) loss of Djrunts function lead to lysis or regeneration failure in the intact and regenerating worms. Overall, the data suggests that Djrunts play important roles in regeneration and homeostatic maintenance in planarians. (Source: Gene Expression Patterns)
Source: Gene Expression Patterns - July 10, 2018 Category: Genetics & Stem Cells Source Type: research
The expression of fgfr3 in the zebrafish head
Publication date: September 2018Source: Gene Expression Patterns, Volume 29Author(s): Joanna K. Ledwon, Sergey Y. Turin, Arun K. Gosain, Jolanta M. TopczewskaAbstractFibroblast growth factor (FGF) signaling is essential for many developmental processes and plays a pivotal role in skeletal homeostasis, regeneration and wound healing. FGF signals through one of five tyrosine kinase receptors: Fgfr1a, -1b, −2, −3, −4. To characterize the expression of zebrafish fgfr3 from the larval stage to adulthood, we used RNAscope in situ hybridization on paraffin sections of the zebrafish head. Our study revealed spatial and tempo...
Source: Gene Expression Patterns - July 10, 2018 Category: Genetics & Stem Cells Source Type: research
The spatial and developmental expression of mouse Vwa8 (von Willebrand domain-containing protein 8)
Publication date: September 2018Source: Gene Expression Patterns, Volume 29Author(s): Brian S. Grewe, Janet E. Richmond, David E. FeatherstoneAbstractThe Drosophila gene c12.2 was isolated in a screen examining mRNA binding proteins. Drosophila c12.2 is the mouse Vwa8 homolog. Various genome-wide associated studies have linked human Vwa8 to both neurological and oncological pathologies, which include autism, bipolar disorder, comorbid migraine, and acute myeloid leukemia, however, the function and role of the VWA8 protein remain poorly understood. To further analyze the Vwa8 gene in mouse, gene structure, protein homology ...
Source: Gene Expression Patterns - July 10, 2018 Category: Genetics & Stem Cells Source Type: research
Identification and expression of carbonic anhydrase 2, myosin regulatory light chain 2 and selenium-binding protein 1 in zebrafish Danio rerio: Implication for age-related biomarkers
In this study we showed that zebrafish ca2, myl2a, myl2b and selenbp1 were highly identical to their mammalian counterparts in primary and tertiary structures as well as genomic organization and syntenic map. They displayed distinct spatiotemporal expression patterns in embryos and larvae of zebrafish. Moreover, their transcription levels in the respective tissues were obviously remodeled in an age-dependent fashion, i.e. some mRNA levels were increased, while others remained unchanged or even decreased, suggesting that CA2, MYL2a, MYL2b and SELENBP1 can be used as aging biomarkers. Our study also lays a foundation for fur...
Source: Gene Expression Patterns - July 10, 2018 Category: Genetics & Stem Cells Source Type: research
The expression of natriuretic peptide receptors in developing zebrafish embryos
In this study, two versions of the npr1 gene (npr1a and npr1b) in zebrafish was identified. Multiple sequences alignment analysis showed that zebrafish NPRs shared high homologies with NPRs of other species and possessed a typical signature domain of NPRs. The results of whole mount in situ hybridization and reverse transcription polymerase chain reaction analysis revealed that at embryonic stages, npr1a was mainly expressed in tectal ventricle, brian, heart and retina, whereas npr1b was broadly present in anterior pronephric duct. Unlike npr1, npr2 mainly expressed in branchial arches and neural tube during embryonic deve...
Source: Gene Expression Patterns - July 10, 2018 Category: Genetics & Stem Cells Source Type: research
The expression of natriuretic peptide receptors in developing zebrafish embryos
In this study, two versions of the npr1 gene (npr1a and npr1b) in zebrafish was identified. Multiple sequences alignment analysis showed that zebrafish NPRs shared high homologies with NPRs of other species and possessed a typical signature domain of NPRs. The results of whole mount in situ hybridization and reverse transcription polymerase chain reaction analysis revealed that at embryonic stages, npr1a was mainly expressed in tectal ventricle, brian, heart and retina, whereas npr1b was broadly present in anterior pronephric duct. Unlike npr1, npr2 mainly expressed in branchial arches and neural tube during embryonic deve...
Source: Gene Expression Patterns - July 7, 2018 Category: Genetics & Stem Cells Source Type: research
Astacin gene family of metalloproteinases in planarians: Structural organization and tissue distribution
Publication date: June 2018Source: Gene Expression Patterns, Volume 28Author(s): Maria Emilia Isolani, Renata Batistoni, Chiara Ippolito, Anna Maria Bianucci, Silvia Marracci, Leonardo RossiAbstractPlanarian flatworms possess extraordinary regenerative capability and body plasticity, which rely on a composite population of stem cells, the neoblasts. Despite impressive advances have been recently achieved in the knowledge of neoblast biology, few is still known about factors that are released by differentiated tissues and influence the neoblast fate. Extracellular matrix (ECM) is a fundamental component of the stem cell nic...
Source: Gene Expression Patterns - July 5, 2018 Category: Genetics & Stem Cells Source Type: research
Editorial Board
Publication date: June 2018Source: Gene Expression Patterns, Volume 28Author(s): (Source: Gene Expression Patterns)
Source: Gene Expression Patterns - July 5, 2018 Category: Genetics & Stem Cells Source Type: research
Oxygen-induced alterations in the expression of chromatin modifying enzymes and the transcriptional regulation of imprinted genes
In this study, we evaluated the capacity of oxygen concentration to influence the transcriptional control of a selection of key enzymes regulating chromatin structure. In mouse embryonic stem cells, oxygen concentrations modulated the transcriptional regulation of the TET family of enzymes, as well as the de novo methyltransferase Dnmt3a. These transcriptional changes were associated with alterations in the control of multiple imprinted genes, including H19, Igf2, Igf2r, and Peg3. Similarly, exposure of in vitro produced bovine embryos to atmospheric oxygen concentrations was associated with disruptions in the transcriptio...
Source: Gene Expression Patterns - July 5, 2018 Category: Genetics & Stem Cells Source Type: research
Early development of the enteric nervous system visualized by using a new transgenic zebrafish line harboring a regulatory region for choline acetyltransferase a (chata) gene
Publication date: June 2018Source: Gene Expression Patterns, Volume 28Author(s): Masataka Nikaido, Saki Izumi, Honoka Ohnuki, Yuki Takigawa, Kyo Yamasu, Kohei HattaAbstractThe enteric nervous system (ENS) is the largest part of the peripheral nervous system in vertebrates. Toward the visualization of the development of the vertebrate ENS, we report our creation of a new transgenic line, Tg(chata:GGFF2) which has a 1.5-kb upstream region of the zebrafish choline acetyltransferase a (chata) gene followed by modified green fluorescent protein (gfp). During development, GFP + cells were detected in the gut by 60 h post-fert...
Source: Gene Expression Patterns - July 5, 2018 Category: Genetics & Stem Cells Source Type: research
Screen for Slit/Robo signaling in trunk neural cells reveals new players
Publication date: June 2018Source: Gene Expression Patterns, Volume 28Author(s): Darwin Martinez, Nora Zuhdi, Michelle Reyes, Blanca Ortega, Dion Giovannone, Vivian M. Lee, Maria Elena de BellardAbstractSlits ligands and their Robo receptors are involved in quite disparate cell signaling pathways that include axon guidance, cell proliferation, cell motility and angiogenesis. Neural crest cells emerge by delamination from neural cells in the dorsal neural tube, and give rise to various components of the peripheral nervous system in vertebrates. It is well established that these cells change from a non-migratory to a highly ...
Source: Gene Expression Patterns - July 5, 2018 Category: Genetics & Stem Cells Source Type: research
