Transcript profiling of salt tolerant tobacco mutants generated via mutation breeding
Publication date: September 2018Source: Gene Expression Patterns, Volume 29Author(s): Özge Çelik, Aybüke Ekşioğlu, Enes Yağız AkdaşAbstractThe main aim of the study is to identify the genes differentially, predominantly or specifically expressed in salt tolerant tobacco mutants, improved from Akhisar 97 and İzmir Özbaş varieties via mutation breeding, with respect to unstressed control plants. Seven tobacco mutants which have different salt tolerance capacities were evaluated by Gene Fishing analysis. Under stress conditions differentially expressed 100 reproducible bands were identified (74 of up-regulated and ...
Source: Gene Expression Patterns - July 5, 2018 Category: Genetics & Stem Cells Source Type: research
The spatial and developmental expression of mouse Vwa8 (von Willebrand domain-containing protein 8)
Publication date: Available online 13 April 2018 Source:Gene Expression Patterns Author(s): Brian S. Grewe, Janet E. Richmond, David E. Featherstone The Drosophila gene c12.2 was isolated in a screen examining mRNA binding proteins. Drosophila c12.2 is the mouse Vwa8 homolog. Various genome-wide associated studies have linked human Vwa8 to both neurological and oncological pathologies, which include autism, bipolar disorder, comorbid migraine, and acute myeloid leukemia, however, the function and role of the VWA8 protein remain poorly understood. To further analyze the Vwa8 gene in mouse, gene structure, protein homol...
Source: Gene Expression Patterns - April 14, 2018 Category: Genetics & Stem Cells Source Type: research
Identification of runt family genes involved in planarian regeneration and tissue homeostasis
In this study, the full-length cDNA sequences of two runt genes are first cloned from Dugesia japonica, and their roles are investigated by WISH and RNAi. The results show that: (1) the Djrunts are conserved during evolution; (2) the Djrunts mRNA are widely expressed in intact and regenerative worms, and their expression levels are up-regulated significantly on day 1 after amputation; (3) loss of Djrunts function lead to lysis or regeneration failure in the intact and regenerating worms. Overall, the data suggests that Djrunts play important roles in regeneration and homeostatic maintenance in planarians. (Source: Gene Expression Patterns)
Source: Gene Expression Patterns - April 10, 2018 Category: Genetics & Stem Cells Source Type: research
Enriched expression of the ciliopathy gene Ick in cell proliferating regions of adult mice
Publication date: Available online 7 April 2018 Source:Gene Expression Patterns Author(s): Ryotaro Tsutsumi, Taro Chaya, Takahisa Furukawa Cilia are essential for sensory and motile functions across species. In humans, ciliary dysfunction causes “ciliopathies”, which show severe developmental abnormalities in various tissues. Several missense mutations in intestinal cell kinase (ICK) gene lead to endocrine-cerebro-osteodysplasia syndrome or short rib-polydactyly syndrome, lethal recessive developmental ciliopathies. We and others previously reported that Ick-deficient mice exhibit neonatal lethality with developme...
Source: Gene Expression Patterns - April 8, 2018 Category: Genetics & Stem Cells Source Type: research
The expression of fgfr3 in the zebrafish head
Publication date: Available online 6 April 2018 Source:Gene Expression Patterns Author(s): Joanna K. Ledwon, Sergey Y. Turin, Arun K. Gosain, Jolanta M. Topczewska Fibroblast growth factor (FGF) signaling is essential for many developmental processes and plays a pivotal role in skeletal homeostasis, regeneration and wound healing. FGF signals through one of five tyrosine kinase receptors: Fgfr1a, -1b, −2, −3, −4. To characterize the expression of zebrafish fgfr3 from the larval stage to adulthood, we used RNAscope in situ hybridization on paraffin sections of the zebrafish head. Our study revealed spatial and t...
Source: Gene Expression Patterns - April 7, 2018 Category: Genetics & Stem Cells Source Type: research
Expression patterns of Yes-associated protein 1 in the developing mouse liver
Publication date: Available online 5 April 2018 Source:Gene Expression Patterns Author(s): Wei Wei, Jeremy Lotto, Pamela A. Hoodless The Hippo signalling pathway regulates many cellular processes, but has been specifically associated with control organ size and tumor growth. Yes-associated protein 1 (YAP1) is a transcriptional cofactor, in the Hippo pathway, that regulates gene expression when localized in the nucleus. Elevated expression of YAP1 in adult mouse liver leads to hepatomegaly and can cause hepatocellular carcinoma; while the loss of function studies reveal its importance in regulating cholangiocyte develo...
Source: Gene Expression Patterns - April 6, 2018 Category: Genetics & Stem Cells Source Type: research
Epigenetic modifications in the embryonic and induced pluripotent stem cells
Publication date: Available online 4 April 2018 Source:Gene Expression Patterns Author(s): Rasoul Godini, Haider Yabr Lafta, Hossein Fallahi Epigenetic modifications are involved in global reprogramming of the cell transcriptome. Therefore, synchronized major shifts in the expression of many genes could be achieved through epigenetic changes. The regulation of gene expression could be implemented by different epigenetic events including histone modifications, DNA methylation and chromatin remodelling. Interestingly, it has been documented that reprogramming of somatic cells to induced pluripotent stem (iPS) cells is a...
Source: Gene Expression Patterns - April 5, 2018 Category: Genetics & Stem Cells Source Type: research
Age-dependent expression pattern in the mammalian brain of a novel, small peptide encoded in the 22q11.2 deletion syndrome region
Publication date: Available online 20 March 2018 Source:Gene Expression Patterns Author(s): Sabrina Kragness, Mark A.A. Harrison, Joby J. Westmoreland, Adam Burstain, Laurie R. Earls 22q11.2 deletion syndrome (22q11DS) carries increased risk for both physical and psychiatric symptoms, including a high risk for schizophrenia. Understanding the genetic elements within the deletion region therefore has the potential to unlock the mysteries of both diseases. While most of the protein-coding genes in this region have been characterized, novel elements, such as non-coding RNAs and small Open Reading Frames (sORFs) remain ...
Source: Gene Expression Patterns - March 20, 2018 Category: Genetics & Stem Cells Source Type: research
Astacin gene family of metalloproteinases in planarians: Structural organization and tissue distribution
Publication date: Available online 13 March 2018 Source:Gene Expression Patterns Author(s): Maria Emilia Isolani, Renata Batistoni, Chiara Ippolito, Anna Maria Bianucci, Silvia Marracci, Leonardo Rossi Planarian flatworms possess extraordinary regenerative capability and body plasticity, which rely on a composite population of stem cells, the neoblasts. Despite impressive advances have been recently achieved in the knowledge of neoblast biology, few is still known about factors that are released by differentiated tissues and influence the neoblast fate. Extracellular matrix (ECM) is a fundamental component of the s...
Source: Gene Expression Patterns - March 14, 2018 Category: Genetics & Stem Cells Source Type: research
Tracheal expression of Osiris gene family in Drosophila
Publication date: Available online 14 March 2018 Source:Gene Expression Patterns Author(s): Aaron Scholl, Yuyang Yang, Patrick McBride, Kelly Irwin, Lan Jiang The Drosophila trachea is a premier genetic system to investigate the fundamental mechanisms of tubular organ formation. Development of the trachea consists of the well understood early branch specification and migration processes, and the less clear later branch maturation process including the apical membrane expansion, cytoskeleton rearrangement, luminal matrix clearance, and air-filling. We identified seven members of the Osiris (Osi) gene family with obvi...
Source: Gene Expression Patterns - March 14, 2018 Category: Genetics & Stem Cells Source Type: research
Investigation of LAMTOR1 gene and protein expressions in germinal vesicle and metaphase II oocytes and embryos from 1-cell to blastocyst stage in a mouse model
In conclusion, both the mRNA and protein levels of LAMTOR1 increase progressively in cleavage-stage mouse embryos. LAMTOR1 has a significant higher embryonic expression at 2-cell to morula stage. LAMTOR1 may play a role in the oogenesis process and probably required for further developmental stages and it may play a possible role in the process of compaction and cavitation in mice. Therefore, further studies are needed to explore the LAMTOR1 expression especially in the different stages of embryonal development. (Source: Gene Expression Patterns)
Source: Gene Expression Patterns - March 4, 2018 Category: Genetics & Stem Cells Source Type: research
Distribution of gonadotropin-inhibitory hormone (GnIH) in male Luchuan piglets
Publication date: Available online 24 February 2018 Source:Gene Expression Patterns Author(s): Xiaoye Wang, Xun Li, Chuanhuo Hu Gonadotropin inhibitory hormone (GnIH) has emerged as a novel hypothalamic neuropeptide that actively inhibits gonadotropin release in birds and mammals. Recent evidence indicates that GnIH not only acts as a key neurohormone that controls vertebrate reproduction but is also involved in stress response, food intake, and aggressive and sexual behaviors, suggesting a broad physiological role for this neuropeptide. To elucidate its multiple sites of action and potential functions, studying the d...
Source: Gene Expression Patterns - February 25, 2018 Category: Genetics & Stem Cells Source Type: research
Molecular characterization, tissue distribution, localization and mRNA expression of the bucky ball gene in the Dabry's sturgeon (Acipenser dabryanus) during oogenesis
In this study, the full-length cDNA of buc homologue in Dabry's sturgeon, Adbuc, was isolated and characterized. Multiple sequence alignments showed that the BUVE domain of Buc was highly conserved in vertebrates, despite exhibiting low identities with each other across the whole protein. By quantitative real-time PCR analysis, we found that Adbuc RNAs were only detected in the gonad with a high level in the ovary and a very low level in the testis. During embryogenesis, these RNAs were highly expressed from the unfertilized eggs to blastula, declined dramatically from the gastrula stage, and hardly found after the neurula...
Source: Gene Expression Patterns - February 24, 2018 Category: Genetics & Stem Cells Source Type: research
Expression of the adhesion G protein-coupled receptor A2 (adgra2) during Xenopus laevis development
In this study, we investigated for the first time the spatio-temporal expression of adgra2 during early Xenopus embryogenesis in detail. In silico approaches showed that the genomic adgra2 region as well as the Adgra2 protein sequence is highly conserved among different species including Xenopus. RT-PCR experiments confirmed that embryonic adgra2 expression is primarily detected at the beginning of neurulation and is then present throughout the whole Xenopus embryogenesis until stage 42. Whole mount in situ hybridization approaches visualized adgra2 expression in many tissues during Xenopus embryogenesis such as the cardio...
Source: Gene Expression Patterns - February 18, 2018 Category: Genetics & Stem Cells Source Type: research
Screen for Slit/Robo signaling in trunk neural cells reveals new players
Publication date: June 2018 Source:Gene Expression Patterns, Volume 28 Author(s): Darwin Martinez, Nora Zuhdi, Michelle Reyes, Blanca Ortega, Dion Giovannone, Vivian M. Lee, Maria Elena de Bellard Slits ligands and their Robo receptors are involved in quite disparate cell signaling pathways that include axon guidance, cell proliferation, cell motility and angiogenesis. Neural crest cells emerge by delamination from neural cells in the dorsal neural tube, and give rise to various components of the peripheral nervous system in vertebrates. It is well established that these cells change from a non-migratory to a high...
Source: Gene Expression Patterns - February 16, 2018 Category: Genetics & Stem Cells Source Type: research
