Expression patterns of homeobox genes in the mouse vomeronasal organ at postnatal stages
Publication date: Available online 9 August 2016 Source:Gene Expression Patterns Author(s): Isabelle Chang, Marta Parrilla Homeodomain proteins are encoded by homeobox genes and regulate development and differentiation in many neuronal systems. The mouse vomeronasal organ (VNO) generates in situ mature chemosensory neurons from stem cells. The roles of homeodomain proteins in neuronal differentiation in the VNO are poorly understood. Here we have characterized the expression patterns of 28 homeobox genes in the VNO of C57BL/6 mice at postnatal stages using multicolor fluorescent in situ hybridization. We identified 11 ...
Source: Gene Expression Patterns - August 9, 2016 Category: Genetics & Stem Cells Source Type: research
Expression of Hox paralog group 13 genes in adult and developing Megalobrama amblycephala
Publication date: Available online 29 July 2016 Source:Gene Expression Patterns Author(s): Ivan Jakovlić, Wei-Min Wang Hox genes encode transcription factors that play a key role in specifying the body plan in most metazoans. HoxPG13 genes most probably played an important role in body length variation during the evolution of animals. This is the first report of the mRNA expression patterns of the entire Hox paralog group 13 (HoxA13a, HoxA13b, HoxB13a, HoxC13a, HoxC13b, HoxD13a) in fish. Expression was studied by qPCR in five tissues of adult Megalobrama amblycephala specimens (spleen, liver, kidney, intestine an...
Source: Gene Expression Patterns - July 29, 2016 Category: Genetics & Stem Cells Source Type: research
Analysis of splice variants for the C. elegans orthologue of human neuroligin reveals a developmentally regulated transcript
Publication date: March 2015 Source:Gene Expression Patterns, Volume 17, Issue 2 Author(s): Fernando Calahorro, Lindy Holden-Dye, Vincent O'Connor Neuroligins are synaptic adhesion molecules and important determinants of synaptic function. They are expressed at postsynaptic sites and involved in synaptic organization through key extracellular and intracellular protein interactions. They undergo trans-synaptic interaction with presynaptic neurexins. Distinct neuroligins use differences in their intracellular domains to selectively recruit synaptic scaffolds and this plays an important role in how they encode speci...
Source: Gene Expression Patterns - July 20, 2016 Category: Genetics & Stem Cells Source Type: research
Quantifying difference in gene expression profile between bovine blastocysts derived by in vitro fertilization and somatic cell nuclear transfer
Publication date: September–November 2015 Source:Gene Expression Patterns, Volume 19, Issues 1–2 Author(s): Sujin Kwon, Sangkyun Jeong, Jung Sun Park, Yong-Kook Kang Epigenetic reprogramming intensely occurs in somatic-cell nuclear transfer (SCNT) embryos, which highlights the importance of proper expressions of reprogramming-related genes in SCNT embryos. We here assessed gene expression profiles (GEPs) difference between bovine blastocyst groups derived by in-vitro fertilization (IVF) or SCNT; in SCNT, cumulus cells and ear skin fibroblasts were used for cSCNT and fSCNT blastocysts, respectively. We obtai...
Source: Gene Expression Patterns - July 20, 2016 Category: Genetics & Stem Cells Source Type: research
The zebrafish fast myosin light chain mylpfa:H2B-GFP transgene is a useful tool for in vivo imaging of myocyte fusion in the vertebrate embryo
Conclusions Since we know so little about the molecules regulating vertebrate myocyte fusion, we propose that the mylpfa:H2B-GFP transgene will be a very useful reporter for conducting forward and reverse genetic screens to identify new components regulating vertebrate myocyte fusion. (Source: Gene Expression Patterns)
Source: Gene Expression Patterns - July 20, 2016 Category: Genetics & Stem Cells Source Type: research
First trimester trophoblasts forming endothelial-like tubes in vitro emulate a ‘blood vessel development’ gene expression profile
Publication date: Available online 21 May 2016 Source:Gene Expression Patterns Author(s): Amanda R. Highet, Sam Buckberry, Benjamin T. Mayne, Sultana M. Khoda, Tina Bianco-Miotto, Claire T. Roberts Extravillous cytotrophoblasts isolated from first trimester placenta, and immortalised cell lines derived from them, have the intrinsic ability to form endothelial-like tubes when cultured on Matrigel™ extracellular matrix. This in vitro tube formation may model placental angiogenesis and/or endovascular differentiation by trophoblasts. To interpret the relevance of this phenomenon to placental development, we...
Source: Gene Expression Patterns - July 20, 2016 Category: Genetics & Stem Cells Source Type: research
Kr üpple-like factors 7 and 6a mRNA expression in adult zebrafish central nervous system
In this study, we used in situ hybridization to examine Klf7 mRNA (klf7) and Klf6a mRNA (klf6a) expression in adult zebrafish CNS. Both klfs exhibit wide and similar expression in the zebrafish CNS. Brain areas containing strongly labeled cells include the ventricular regions of the dorsomedial telencephalon, the ventromedial telencephalon, periventricular regions of the thalamus and hypothalamus, torus longitudinalis, stratum periventriculare of the optic tectum, granular regions of the cerebellar body and valvula, and superficial layers of the facial and vagal lobes. In the spinal cord, klf7- and klf6a-expressing cells a...
Source: Gene Expression Patterns - July 20, 2016 Category: Genetics & Stem Cells Source Type: research
Wnt, Frizzled, and sFRP gene expression patterns during gastrulation in the starfish Patiria (Asterina) pectinifera
Publication date: Available online 23 June 2016 Source:Gene Expression Patterns Author(s): Narudo Kawai, Ritsu Kuraishi, Hiroyuki Kaneko By the initial phase of gastrulation, Wnt pathway regulation mediates endomesoderm specification and establishes the animal-vegetal axis, thereby leading to proper gastrulation in starfish. To provide insight into the ancestral mechanism regulating deuterostome gastrulation, we identified the gene expression patterns of Wnt, Frizzled (Fz), and secreted frizzled-related protein (sFRP) family genes, which play a role in the initial stage of the Wnt pathway, in starfish Patiria (As...
Source: Gene Expression Patterns - July 4, 2016 Category: Genetics & Stem Cells Source Type: research
Krüpple-like factors 7 and 6a mRNA expression in adult zebrafish central nervous system
In this study, we used in situ hybridization to examine Klf7 mRNA (klf7) and Klf6a mRNA (klf6a) expression in adult zebrafish CNS. Both klfs exhibit wide and similar expression in the zebrafish CNS. Brain areas containing strongly labeled cells include the ventricular regions of the dorsomedial telencephalon, the ventromedial telencephalon, periventricular regions of the thalamus and hypothalamus, torus longitudinalis, stratum periventriculare of the optic tectum, granular regions of the cerebellar body and valvula, and superficial layers of the facial and vagal lobes. In the spinal cord, klf7- and klf6a-expressing cells a...
Source: Gene Expression Patterns - July 4, 2016 Category: Genetics & Stem Cells Source Type: research
Analysis of anthocyanin biosynthesis genes expression profiles in contrasting cultivars of Japanese plum (Prunus salicina L.) during fruit development
Publication date: Available online 1 July 2016 Source:Gene Expression Patterns Author(s): Máximo González, Erika Salazar, Soledad Cabrera, Pilar Olea, Basilio Carrasco Flavonoids are responsible of different fruit sensorial properties. In Japanese plum (Prunus salicina L.) these compounds are variable in both type and quantity during the different stages of fruit growth and maturation. Here we present the first study which determines the expression profile of structural genes of the flavonoid pathway and accumulation profiles of total phenols, proanthocyanidins and anthocyanins during fruit development stag...
Source: Gene Expression Patterns - July 4, 2016 Category: Genetics & Stem Cells Source Type: research
An in situ hybridization study of Hyaluronan synthase (Has) mRNA in developing mouse molar and incisor tooth germs
Publication date: Available online 8 June 2016 Source:Gene Expression Patterns Author(s): Tsuyoshi Morita, Kaoru Fujikawa, Otto Baba, Shunichi Shibata Hyaluronan (HA) is a major constituent molecule in most extracellular matrices and is synthesized by Hyaluronan synthase (Has). In the present study, we examined expression patterns of Has1, -2, -3 mRNA in developing mouse molar and incisor tooth germs from embryonic day (E) 11.5 to postnatal day (P) 7, focusing on Hertwig's epithelial root sheath (HERS) and the apical bud in particular. Has1 mRNA expression was not detected in all tooth germs examined. Has2 mRNA...
Source: Gene Expression Patterns - June 7, 2016 Category: Genetics & Stem Cells Source Type: research
Spatiotemporal expression pattern of the zebrafish aquaporin 8 family during early developmental stages
Publication date: Available online 3 June 2016 Source:Gene Expression Patterns Author(s): Soonil Koun, Jun-Dae Kim, Myungchull Rhee, Myoung-Jin Kim, Tae-Lin Huh Aquaporin 8 (Aqp 8) is a transmembrane protein that is selectively permeated by water and some small solutes, and physiologically contributes to acid-base equilibrium in the gastrointestinal tract. Here, we described the characterization and spatiotemporal expression pattern of zebrafish aqp8 (zaqp8) gene family, including zaqp8a.1, zaqp8a.2, and zaqp8b, during the early developmental stages. The expression of zaqp8a.1 started first in the lateral pla...
Source: Gene Expression Patterns - June 2, 2016 Category: Genetics & Stem Cells Source Type: research
Molecular characterization and developmental expression patterns of apolipoprotein A-I in Senegalese sole (Solea senegalensis Kaup)
In this study, the cDNA and genomic sequences of this apolipoprotein were characterized for first time in S. senegalensis. The predicted polypeptide revealed conserved structural features including ten repeats in the lipid-binding domain and some residues involved in cholesterol interaction and binding. The gene structure analysis identified four exons and three introns. Moreover, the synteny analysis revealed that apoA-I did not localize with other apolipoproteins indicating a divergent evolution with respect to the apoA-IV and apoE cluster. The phylogenetic analyses identified two distinct apoA-I paralogs in Ostariophysi...
Source: Gene Expression Patterns - May 31, 2016 Category: Genetics & Stem Cells Source Type: research
Spatio-temporal expression of Sox genes in murine palatogenesis
Publication date: Available online 27 May 2016 Source:Gene Expression Patterns Author(s): Momoko Waranabe, Katsushige Kawasaki, Maiko Kawasaki, Thantrira Portaveetus, Shelly Oommen, James Blackburn, Takahiro Nagai, Atsushi Kitatmura, Atsushi Nishikawa, Yasumitsu Kodama, Ritsuo Takagi, Takeyasu Maeda, Paul T. Sharpe, Atsushi Ohazama Members of the Sox gene family play critical roles in many biological processes including organogenesis. We carried out comparative in situ hybridization analysis of seventeen Sox genes (Sox1-14, 17, 18 and 21) during murine palatogenesis from initiation to fusion...
Source: Gene Expression Patterns - May 27, 2016 Category: Genetics & Stem Cells Source Type: research
First trimester trophoblasts forming endothelial-like tubes in vitro emulate a ‘blood vessel development’ gene expression profile
Publication date: Available online 21 May 2016 Source:Gene Expression Patterns Author(s): Amanda R. Highet, Sam Buckberry, Benjamin T. Mayne, Sultana M. Khoda, Tina Bianco-Miotto, Claire T. Roberts Extravillous cytotrophoblasts isolated from first trimester placenta, and immortalised cell lines derived from them, have the intrinsic ability to form endothelial-like tubes when cultured on Matrigel™ extracellular matrix. This in vitro tube formation may model placental angiogenesis and/or endovascular differentiation by trophoblasts. To interpret the relevance of this phenomenon to placental development, we...
Source: Gene Expression Patterns - May 25, 2016 Category: Genetics & Stem Cells Source Type: research
