The expression patterns of vestigial like family member 4 genes in zebrafish embryogenesis
In this study, we used in situ hybridization to elucidate the temporal and spatial expression of zebrafish vgll4 paralogs during normal embryonic and larval development. Similar expression was shown in certain areas at similar stages for the three paralogs. Expression of vgll4a, vgll4b and vgll4l were all found in pectoral fins and otic vesicles during the early developmental stages. On the other hand, a few differences of the three paralogs expression were found in eyes, pharynx, pharyngeal arches and brain tissues. The expression of vgll4a was weak and ubiquitous, while vgll4b was obviously expressed in brain tissues and...
Source: Gene Expression Patterns - February 15, 2018 Category: Genetics & Stem Cells Source Type: research
Early development of the enteric nervous system visualized by using a new transgenic zebrafish line harboring a regulatory region for choline acetyltransferase a (chata) gene
Publication date: June 2018 Source:Gene Expression Patterns, Volume 28 Author(s): Masataka Nikaido, Saki Izumi, Honoka Ohnuki, Yuki Takigawa, Kyo Yamasu, Kohei Hatta The enteric nervous system (ENS) is the largest part of the peripheral nervous system in vertebrates. Toward the visualization of the development of the vertebrate ENS, we report our creation of a new transgenic line, Tg(chata:GGFF2) which has a 1.5-kb upstream region of the zebrafish choline acetyltransferase a (chata) gene followed by modified green fluorescent protein (gfp). During development, GFP + cells were detected in the gut by 60 h post-fe...
Source: Gene Expression Patterns - February 8, 2018 Category: Genetics & Stem Cells Source Type: research
Oxygen-induced alterations in the expression of chromatin modifying enzymes and the transcriptional regulation of imprinted genes
In this study, we evaluated the capacity of oxygen concentration to influence the transcriptional control of a selection of key enzymes regulating chromatin structure. In mouse embryonic stem cells, oxygen concentrations modulated the transcriptional regulation of the TET family of enzymes, as well as the de novo methyltransferase Dnmt3a. These transcriptional changes were associated with alterations in the control of multiple imprinted genes, including H19, Igf2, Igf2r, and Peg3. Similarly, exposure of in vitro produced bovine embryos to atmospheric oxygen concentrations was associated with disruptions in the transcriptio...
Source: Gene Expression Patterns - February 8, 2018 Category: Genetics & Stem Cells Source Type: research
Cloning and gene expression analysis of two cDNA of cysteine proteinase genes involved in programmed cell death in the inner integument from developing seeds of Jatropha curcas L
Publication date: Available online 19 December 2017 Source:Gene Expression Patterns Author(s): Antônio José Rocha, Simone Pohl, Cristiane Sá Roriz Fonteles In this paper, two cysteine proteinases were cloned from Jatropha curcas seeds. The full length cDNAs obtained from cloning of Jc-CysEP1 and Jc-CysEP2 genes were 1.516bp and 1500 pb, respectively. The Jc-CysEP1 contained a 1083bp open reading frame (ORF) coding for 360 amino acids. The JcCysEP1 protein sequence had an estimated native molecular weight of 36.89 kDa, with a predicted isoelectric point of 4.55. The average lengths of JcCysEP1 5′ UTR and 3′ ...
Source: Gene Expression Patterns - December 19, 2017 Category: Genetics & Stem Cells Source Type: research
Tissue specific expression and in-silico characterization of a putative Cysteine synthase gene from Lathyrus sativus L.
Publication date: Available online 13 December 2017 Source:Gene Expression Patterns Author(s): Saikat Chakraborty, Joy Mitra, Milan Kumar Samanta, Narattam Sikdar, Jagannath Bhattacharyya, Anulina Manna, Subrata Pradhan, Anirban Chakraborty, Bikas Ranjan Pati Grass pea (Lathyrus sativus L.) is a worldwide popular pulse crop especially for its protein rich seeds with least production cost. However, the use of the crop became controversial due to the presence of non-protein amino acid, β-N-oxalyl-L-α, β-diaminopropionic acid (β-ODAP) in its seed and leaf, which is known as the principle neurotoxin to cause neu...
Source: Gene Expression Patterns - December 14, 2017 Category: Genetics & Stem Cells Source Type: research
The dynamics of native Atoh7 protein expression during mouse retinal histogenesis, revealed with a new antibody
Publication date: Available online 7 December 2017 Source:Gene Expression Patterns Author(s): Joel B. Miesfeld, Tom Glaser, Nadean L. Brown The Atoh7 transcription factor catalyzes the rate-limiting step in the specification of retinal ganglion cells (RGCs). As a tool to study vertebrate retinal development, we validate an antibody that recognizes human and mouse Atoh7 polypeptide, using informative knockout and transgenic mouse tissues and overexpression experiments. The transient features of Atoh7 protein expression during retinal neurogenesis match the expected pattern at the tissue and cellular level. Further, we ...
Source: Gene Expression Patterns - December 8, 2017 Category: Genetics & Stem Cells Source Type: research
Comparative expression analyses of bone morphogenetic protein 4 (BMP4) expressions in muscles of tilapia and common carp indicate that BMP4 plays a role in the intermuscular bone distribution in a dose-dependent manner
Publication date: Available online 26 November 2017 Source:Gene Expression Patterns Author(s): Shengyan Su, Zaijie Dong Intermuscular bones in fish negatively influence both meet processing and attractiveness to consumers. Tilapia (Oreochromis niloticus) and common carp (Cyprinus carpio) are both major farmed fish species globally, but whereas the former does not possess intermuscular bones, the latter does. Therefore, these two species might present a good model to study the genetic control of distribution of intermuscular bones in fish. Bone morphogenetic protein 4 (BMP4) gene is associated with tissue ossification a...
Source: Gene Expression Patterns - December 6, 2017 Category: Genetics & Stem Cells Source Type: research
Hey1 and Hey2 are differently expressed during mouse tooth development
Publication date: January 2018 Source:Gene Expression Patterns, Volume 27 Author(s): Kotono Kibe, Mitsushiro Nakatomi, Shinji Kataoka, Takashi Toyono, Yuji Seta The Hey family (also known as Chf, Herp, Hesr, and Hrt) is a set of Hairy/Enhancer of Split-related basic helix-loop-helix type transcription factors. Hey1, Hey2, and HeyL have been identified in mammals. Although Hey proteins are known to regulate cardiovascular development, muscle homeostasis, osteogenesis, neurogenesis, and oncogenesis, their roles in tooth development have been largely obscure. Therefore, this study aimed to clarify detailed spatiotempor...
Source: Gene Expression Patterns - December 6, 2017 Category: Genetics & Stem Cells Source Type: research
Asymmetric distribution of CRUMBS polarity complex proteins from compacted 8-cell to blastocyst stage during mouse preimplantation development
This study investigated the early developmental temporal and spatial expression of the main CRUMBS polarity complex proteins in the mouse preimplantation embryo. We observed that Crb3, Pals1, Patj and Mpdz are transcribed in the mouse preimplantation embryo. However, the asymmetric distribution of these polarity proteins is not established until the compacted 8-cell stage. From compaction and thereafter, CRB3 and PALS1 are progressively enriched in the apical membrane, while PATJ and MPDZ are discretely localized at both tight junctions and the apical membrane adjacent to tight junctions. These temporal and spatial distrib...
Source: Gene Expression Patterns - November 17, 2017 Category: Genetics & Stem Cells Source Type: research
Spatiotemporal expression pattern of Connexin 43 during early chick embryogenesis
Publication date: Available online 7 November 2017 Source:Gene Expression Patterns Author(s): Karyn Jourdeuil, Lisa A. Taneyhill During embryogenesis, a single cell develops into new tissues and organs that are made up of a number of different cell types. The assembly of the trigeminal ganglion (cranial nerve V), an important component of the peripheral nervous system, typifies this process. The trigeminal ganglia perform key sensory functions, including sensing pain and touch in the face, and arise from cells of two different progenitor populations, the neural crest and the cranial placodes. One question that remains ...
Source: Gene Expression Patterns - November 8, 2017 Category: Genetics & Stem Cells Source Type: research
Identification and characterization of molluscan caveolin-1 ortholog from Haliotis discus discus: Possible involvement in embryogenesis and host defense mechanism against pathogenic stress
In this study, we identified and characterized CAV1 ortholog from the disk abalone, Haliotis discus discus (HdCAV1). The cDNA sequence of HdCAV1 is 826 bp long and encodes a 127-amino acid polypeptide. Characteristic caveolin superfamily domain (Glu3 – Lys126) and two possible transmembrane domains (Cys48 - Tyr67 and Ile103 - Phe120) were identified in the HdCAV1 protein. Homology analysis revealed that HdCAV1 shared higher identity (>47%) with molluscans, but lower identity with other species. Phylogenetic tree constructed by the neighbor-joining (NJ) method revealed a distinct evolutionary pathway for molluscans...
Source: Gene Expression Patterns - November 8, 2017 Category: Genetics & Stem Cells Source Type: research
High-throughput method for extracting and visualizing the spatial gene expressions from in situ hybridization images: A case study of the early development of the sea anemone Nematostella vectensis
Publication date: Available online 6 November 2017 Source:Gene Expression Patterns Author(s): A.M. Abdol, Andrew Bedard, Imke Lansky, J.A. Kaandorp Studying the spatial gene expression profiles from in situ hybridization images of the embryo is one of the first steps toward the comprehensive understanding of gene interactions in an organism. In the case of N. vectensis, extracting and collecting these data is a challenging task due to the difficulty of detecting the cell layer through the transparent body plan and changing morphology during the blastula and gastrula stages. Here, first, we introduce a method to algor...
Source: Gene Expression Patterns - November 6, 2017 Category: Genetics & Stem Cells Source Type: research
A six-gene expression toolbox for the glands, epithelium and chondrocytes in the mouse nasal cavity
Publication date: Available online 6 November 2017 Source:Gene Expression Patterns Author(s): Yong Wan, Matthew B. Rogers, Heather L. Szabo-Rogers The nose is the central feature of the amniote face. In adults, the nose is a structurally and functionally complex organ that consists of bone, cartilage, glands and ducts. In an ongoing expression screen in our lab, we found several novel markers for specific tissues in the nasal region. Here, using in situ hybridization expression experiments, we report that Alx1, Ap-2β, Crispld1, Eya4, Moxd1, and Penk have tissue specific expression during murine nasal development. At ...
Source: Gene Expression Patterns - November 6, 2017 Category: Genetics & Stem Cells Source Type: research
Neuronal expression patterns of the PlexinA family during zebrafish development
Publication date: Available online 28 October 2017 Source:Gene Expression Patterns Author(s): Sarah E. Emerson, Sarah E. Light, Alicia M. Ebert Plexins (Plxns) and Semaphorins (Semas) are key signaling molecules that regulate many aspects of development. Plxns are a family of transmembrane protein receptors that are activated upon extracellular binding by Semas. Activated Plxns trigger intracellular signaling cascades, which regulate a range of developmental processes, including axon guidance, neuronal positioning and vasculogenesis. Semas are a large family of both transmembrane and secreted signaling molecules, and ...
Source: Gene Expression Patterns - October 29, 2017 Category: Genetics & Stem Cells Source Type: research
Postimplantation Mga expression and embryonic lethality of two gene-trap alleles
Conclusions Widespread yet specific areas of expression of Mga during organogenesis raise the possibility that the transcription factor may play roles in controlling proliferation and potency in the progenitor cell populations of different organ systems. Documentation of these patterns sets the stage for the investigation of specific progenitor cell types. (Source: Gene Expression Patterns)
Source: Gene Expression Patterns - October 27, 2017 Category: Genetics & Stem Cells Source Type: research
