Molecular characterization of wdr68 gene in embryonic development of Xenopus laevis
Publication date: Available online 17 August 2018Source: Gene Expression PatternsAuthor(s): M. Bonano, E. Martín, M.M. Moreno Ruiz Holgado, G.M. Silenzi Usandivaras, G. Ruiz De Bigliardo, M.J. AybarAbstractWDR68, also known as DCAF7, is a WD40 repeated domain protein highly conserved in eukaryotic organisms in both plants and animals. This protein participates in numerous cellular processes and exerts its function through interaction with other proteins. In the present work, we isolated, sequenced and characterized cDNA corresponding to the wdr68 gene in embryos of the amphibian Xenopus laevis. Syntenic analysis revealed ...
Source: Gene Expression Patterns - August 18, 2018 Category: Genetics & Stem Cells Source Type: research
Developmental expression of the zebrafish Arf-like small GTPase paralogs arl13a and arl13b
Publication date: September 2018Source: Gene Expression Patterns, Volume 29Author(s): Ping Song, Brian D. PerkinsAbstractMembers of the Arf-like (Arl) family of small GTP-binding proteins regulate a number of cellular functions and play important roles in cilia structure and signaling. The small GTPase Arl13a is a close paralog to Arl13b, a small GTPase required for normal cilia formation that causes Joubert Syndrome when mutated. As mutation of arl13b causes a slow retinal degeneration in zebrafish (Song et al., 2016), we hypothesized that expression of arl13a may provide functional redundancy. We determined the expressio...
Source: Gene Expression Patterns - July 18, 2018 Category: Genetics & Stem Cells Source Type: research
Astacin gene family of metalloproteinases in planarians: Structural organization and tissue distribution
Publication date: June 2018Source: Gene Expression Patterns, Volume 28Author(s): Maria Emilia Isolani, Renata Batistoni, Chiara Ippolito, Anna Maria Bianucci, Silvia Marracci, Leonardo RossiAbstractPlanarian flatworms possess extraordinary regenerative capability and body plasticity, which rely on a composite population of stem cells, the neoblasts. Despite impressive advances have been recently achieved in the knowledge of neoblast biology, few is still known about factors that are released by differentiated tissues and influence the neoblast fate. Extracellular matrix (ECM) is a fundamental component of the stem cell nic...
Source: Gene Expression Patterns - July 10, 2018 Category: Genetics & Stem Cells Source Type: research
Editorial Board
Publication date: June 2018Source: Gene Expression Patterns, Volume 28Author(s): (Source: Gene Expression Patterns)
Source: Gene Expression Patterns - July 10, 2018 Category: Genetics & Stem Cells Source Type: research
Oxygen-induced alterations in the expression of chromatin modifying enzymes and the transcriptional regulation of imprinted genes
In this study, we evaluated the capacity of oxygen concentration to influence the transcriptional control of a selection of key enzymes regulating chromatin structure. In mouse embryonic stem cells, oxygen concentrations modulated the transcriptional regulation of the TET family of enzymes, as well as the de novo methyltransferase Dnmt3a. These transcriptional changes were associated with alterations in the control of multiple imprinted genes, including H19, Igf2, Igf2r, and Peg3. Similarly, exposure of in vitro produced bovine embryos to atmospheric oxygen concentrations was associated with disruptions in the transcriptio...
Source: Gene Expression Patterns - July 10, 2018 Category: Genetics & Stem Cells Source Type: research
Early development of the enteric nervous system visualized by using a new transgenic zebrafish line harboring a regulatory region for choline acetyltransferase a (chata) gene
Publication date: June 2018Source: Gene Expression Patterns, Volume 28Author(s): Masataka Nikaido, Saki Izumi, Honoka Ohnuki, Yuki Takigawa, Kyo Yamasu, Kohei HattaAbstractThe enteric nervous system (ENS) is the largest part of the peripheral nervous system in vertebrates. Toward the visualization of the development of the vertebrate ENS, we report our creation of a new transgenic line, Tg(chata:GGFF2) which has a 1.5-kb upstream region of the zebrafish choline acetyltransferase a (chata) gene followed by modified green fluorescent protein (gfp). During development, GFP + cells were detected in the gut by 60 h post-fert...
Source: Gene Expression Patterns - July 10, 2018 Category: Genetics & Stem Cells Source Type: research
Screen for Slit/Robo signaling in trunk neural cells reveals new players
Publication date: June 2018Source: Gene Expression Patterns, Volume 28Author(s): Darwin Martinez, Nora Zuhdi, Michelle Reyes, Blanca Ortega, Dion Giovannone, Vivian M. Lee, Maria Elena de BellardAbstractSlits ligands and their Robo receptors are involved in quite disparate cell signaling pathways that include axon guidance, cell proliferation, cell motility and angiogenesis. Neural crest cells emerge by delamination from neural cells in the dorsal neural tube, and give rise to various components of the peripheral nervous system in vertebrates. It is well established that these cells change from a non-migratory to a highly ...
Source: Gene Expression Patterns - July 10, 2018 Category: Genetics & Stem Cells Source Type: research
Age-dependent expression pattern in the mammalian brain of a novel, small peptide encoded in the 22q11.2 deletion syndrome region
Publication date: June 2018Source: Gene Expression Patterns, Volume 28Author(s): Sabrina Kragness, Mark A.A. Harrison, Joby J. Westmoreland, Adam Burstain, Laurie R. EarlsAbstract22q11.2 deletion syndrome (22q11.2DS) carries increased risk for both physical and psychiatric symptoms, including a high risk for schizophrenia. Understanding the genetic elements within the deletion region therefore has the potential to unlock the mysteries of both diseases. While most of the protein-coding genes in this region have been characterized, novel elements, such as non-coding RNAs and small Open Reading Frames (sORFs) remain unstudied...
Source: Gene Expression Patterns - July 10, 2018 Category: Genetics & Stem Cells Source Type: research
Distribution of gonadotropin-inhibitory hormone (GnIH) in male Luchuan piglets
Publication date: June 2018Source: Gene Expression Patterns, Volume 28Author(s): Xiaoye Wang, Xun Li, Chuanhuo HuAbstractGonadotropin inhibitory hormone (GnIH) has emerged as a novel hypothalamic neuropeptide that actively inhibits gonadotropin release in birds and mammals. Recent evidence indicates that GnIH not only acts as a key neurohormone that controls vertebrate reproduction but is also involved in stress response, food intake, and aggressive and sexual behaviors, suggesting a broad physiological role for this neuropeptide. To elucidate its multiple sites of action and potential functions, studying the detailed dist...
Source: Gene Expression Patterns - July 10, 2018 Category: Genetics & Stem Cells Source Type: research
Expression of the adhesion G protein-coupled receptor A2 (adgra2) during Xenopus laevis development
In this study, we investigated for the first time the spatio-temporal expression of adgra2 during early Xenopus embryogenesis in detail. In silico approaches showed that the genomic adgra2 region as well as the Adgra2 protein sequence is highly conserved among different species including Xenopus. RT-PCR experiments confirmed that embryonic adgra2 expression is primarily detected at the beginning of neurulation and is then present throughout the whole Xenopus embryogenesis until stage 42. Whole mount in situ hybridization approaches visualized adgra2 expression in many tissues during Xenopus embryogenesis such as the cardio...
Source: Gene Expression Patterns - July 10, 2018 Category: Genetics & Stem Cells Source Type: research
Molecular characterization, tissue distribution, localization and mRNA expression of the bucky ball gene in the Dabry's sturgeon (Acipenser dabryanus) during oogenesis
In this study, the full-length cDNA of buc homologue in Dabry's sturgeon, Adbuc, was isolated and characterized. Multiple sequence alignments showed that the BUVE domain of Buc was highly conserved in vertebrates, despite exhibiting low identities with each other across the whole protein. By quantitative real-time PCR analysis, we found that Adbuc RNAs were only detected in the gonad with a high level in the ovary and a very low level in the testis. During embryogenesis, these RNAs were highly expressed from the unfertilized eggs to blastula, declined dramatically from the gastrula stage, and hardly found after the neurula...
Source: Gene Expression Patterns - July 10, 2018 Category: Genetics & Stem Cells Source Type: research
Investigation of LAMTOR1 gene and protein expressions in germinal vesicle and metaphase II oocytes and embryos from 1-cell to blastocyst stage in a mouse model
In conclusion, both the mRNA and protein levels of LAMTOR1 increase progressively in cleavage-stage mouse embryos. LAMTOR1 has a significant higher embryonic expression at 2-cell to morula stage. LAMTOR1 may play a role in the oogenesis process and probably required for further developmental stages and it may play a possible role in the process of compaction and cavitation in mice. Therefore, further studies are needed to explore the LAMTOR1 expression especially in the different stages of embryonal development. (Source: Gene Expression Patterns)
Source: Gene Expression Patterns - July 10, 2018 Category: Genetics & Stem Cells Source Type: research
Tracheal expression of Osiris gene family in Drosophila
Publication date: June 2018Source: Gene Expression Patterns, Volume 28Author(s): Aaron Scholl, Yuyang Yang, Patrick McBride, Kelly Irwin, Lan JiangAbstractThe Drosophila trachea is a premier genetic system to investigate the fundamental mechanisms of tubular organ formation. Development of the trachea consists of the well understood early branch specification and migration processes, and the less clear later branch maturation process including the apical membrane expansion, cytoskeleton rearrangement, luminal matrix clearance, and air-filling. We identified seven members of the Osiris (Osi) gene family with obvious trachea...
Source: Gene Expression Patterns - July 10, 2018 Category: Genetics & Stem Cells Source Type: research
The expression patterns of vestigial like family member 4 genes in zebrafish embryogenesis
In this study, we used in situ hybridization to elucidate the temporal and spatial expression of zebrafish vgll4 paralogs during normal embryonic and larval development. Similar expression was shown in certain areas at similar stages for the three paralogs. Expression of vgll4a, vgll4b and vgll4l were all found in pectoral fins and otic vesicles during the early developmental stages. On the other hand, a few differences of the three paralogs expression were found in eyes, pharynx, pharyngeal arches and brain tissues. The expression of vgll4a was weak and ubiquitous, while vgll4b was obviously expressed in brain tissues and...
Source: Gene Expression Patterns - July 10, 2018 Category: Genetics & Stem Cells Source Type: research
ADAMTS9, a member of the ADAMTS family, in Xenopus development
In this study we identified the 19 members of the ADAMTS family in Xenopus laevis and Xenopus tropicalis. Gene identification and a phylogenetic study revealed strong conservation of the ADAMTS family and contributed to a better annotation of the Xenopus genomes. Expression of the entire ADAMTS family was studied from early stages to tadpole stages of Xenopus, and detailed analysis of ADAMTS9 revealed expression in many structures during organogenesis such as neural crest (NC) derivative tissues, the pronephros and the pancreas. Versican, a matrix component substrate of ADAMTS9 shows a similar expression pattern suggesting...
Source: Gene Expression Patterns - July 10, 2018 Category: Genetics & Stem Cells Source Type: research
