Lateralization of housekeeping genes in the brain of one-day old chicks
This study evaluated the expression stability of eleven candidate housekeeping genes in the IMM region of the 1-day old chick brain. In our experimental system, the most reliable results were given by the NormFinder algorithm. The results show for the first time that ACTB, commonly used as an avian reference gene, is not suitable for investigation of gene expression in the chick brain and that brain lateralization exact selection of different reference gens for each hemisphere. For memory process investigations using tasks in one-day old chicks the most effective reference genes for the left hemisphere were HMBS and SDHA, ...
Source: Gene Expression Patterns - June 16, 2017 Category: Genetics & Stem Cells Source Type: research
Expression analyses of salinity stress- associated ESTs in Aeluropus littoralis
Publication date: Available online 15 June 2017 Source:Gene Expression Patterns Author(s): Tayebeh Kakeshpour, Sajad Rashidi Monfared, Amin Ebrahimi, Arman Beyraghdar Kashkooli, Esmaeil Ebrahimie Salinity is among the most important abiotic stresses affecting crop production throughout the earth. Halophyte plants can sustain high salinity levels, therefore elucidating molecular mechanisms underlying their salinity resistance is beneficial for crop improvement. Aeluropus littoralis, a halophyte weed, is a great genetic resource for this purpose. Isolated expressed sequence taq (EST) sequences from A. littoralis under...
Source: Gene Expression Patterns - June 16, 2017 Category: Genetics & Stem Cells Source Type: research
Differential expression of long noncoding RNAs in congenital microtia
Conclusion Differential expression of lncRNAs occurs in microtia. These lncRNAs and related signalling pathways may play an important role in the occurrence and development ofmicrotia. (Source: Gene Expression Patterns)
Source: Gene Expression Patterns - June 16, 2017 Category: Genetics & Stem Cells Source Type: research
Conservation of BMP2/4 expression patterns within the clade Branchiostoma (amphioxus): Resolving interspecific discrepancies
Publication date: Available online 15 June 2017 Source:Gene Expression Patterns Author(s): Luok Wen Yong, Stéphanie Bertrand, Jr-Kai Yu, Hector Escriva, Nicholas D. Holland In 2016, Kaji et al. concluded that the amphioxus mouth has the quality of a coelomoduct and is, therefore, not homologous to the oral opening of any other animal. They studied a Japanese population of Branchiostoma japonicum and based their conclusion, in part, on the larval expression of BMP2/4 in cells that reportedly joined the rim of the forming mouth. They did not detect transcription of that gene in any other tissues in the anterior regio...
Source: Gene Expression Patterns - June 16, 2017 Category: Genetics & Stem Cells Source Type: research
Embryonic expression patterns of Eukaryotic EndoU ribonuclease family gene endouC in zebrafish
Publication date: Available online 10 June 2017 Source:Gene Expression Patterns Author(s): Hung-Chieh Lee, Chuan-Yang Fu, Chih-Wei Zeng, Huai-Jen Tsai Endou proteins belong to the Eukaryotic EndoU ribonuclease family of enzymes that present high sequence homology with the founding member XendoU domain. The enzymatic activity and three-dimensional structure of some Endou proteins have been previously reported. However, their molecular structure and gene expression patterns during embryogenesis remain to be elucidated. Therefore, we took zebrafish (Danio rerio) endouC as the model to study molecular structure and gene ...
Source: Gene Expression Patterns - June 10, 2017 Category: Genetics & Stem Cells Source Type: research
Deciphering the role of microRNA – A step by step guide
Publication date: Available online 8 June 2017 Source:Gene Expression Patterns Author(s): Siddharth Manvati, Kailash Chandra Mangalhara, Juveria Khan, Geeta Lal Pathania, Srishti Kaul, Monika Kaushik, Ankita Arora, Pawan K. Dhar MicroRNAs (miRNAs), are small non-coding RNAs of approximately 22 nucleotides in length, playing an important role in regulating gene expression post-transcriptionally. Understanding the effect of miRNA regulation in a pathway-specific manner unravels the approaches adopted to apprehend biological mechanisms, the information, which is scanty for researchers, not primed already for miR rel...
Source: Gene Expression Patterns - June 8, 2017 Category: Genetics & Stem Cells Source Type: research
Gene expression profiles in mouse cumulus cells derived from in vitro matured oocytes with and without blastocyst formation
Publication date: November 2017 Source:Gene Expression Patterns, Volumes 25–26 Author(s): Yixin Xu, Tuanping Zhou, Li Shao, Bei Zhang, Kailu Liu, Chao Gao, Li Gao, Jiayin Liu, Yugui Cui, Ri-Cheng Chian Cumulus cells (CCs) are considered as an important source to predict oocyte quality. Despite numerous candidate genes in CCs have been identified for embryonic developmental competence, the results are inconsistent. The next generation RNA-sequencing was used to investigate the transcriptomic differences in CCs from in vitro matured oocytes did or did not develop to blastocyst stage following in vitro fertili...
Source: Gene Expression Patterns - June 8, 2017 Category: Genetics & Stem Cells Source Type: research
Gene expression profiles in mouse cumulus cells derived from in vitro matured oocytes with and without blastocyst formation
Publication date: Available online 2 June 2017 Source:Gene Expression Patterns Author(s): Yixin Xu, Tuanping Zhou, Li Shao, Zhang Bei, Kailu Liu, Chao Gao, Li Gao, Jiayin Liu, Yugui Cui, Ri-Cheng Chian Cumulus cells (CCs) are considered as an important source to predict oocyte quality. Despite numerous candidate genes in CCs have been identified for embryonic developmental competence, the results are inconsistent. The next generation RNA-sequencing was used to investigate the transcriptomic differences in CCs from in vitro matured oocytes did or did not develop to blastocyst stage following in vitro fertilizati...
Source: Gene Expression Patterns - June 2, 2017 Category: Genetics & Stem Cells Source Type: research
Regional localization of activin- βA, activin-βC, follistatin, proliferation, and apoptosis in adult and developing mouse prostate ducts
In conclusion, the present study provides evidence to support a role of activin-C in prostate development and provides new insights in the spatiotemporal localization of activins and their antagonists during mouse prostate development. (Source: Gene Expression Patterns)
Source: Gene Expression Patterns - May 24, 2017 Category: Genetics & Stem Cells Source Type: research
High resolution methylation analysis of the HoxA5 regulatory region in different somatic tissues of laboratory mouse during development
Publication date: January 2017 Source:Gene Expression Patterns, Volumes 23–24 Author(s): Puja Sinha, Kiran Singh, Manisha Sachan Homeobox genes encode a group of DNA binding regulatory proteins whose key function occurs in the spatial-temporal organization of genome during embryonic development and differentiation. The role of these Hox genes during ontogenesis makes it an important model for research. HoxA5 is a member of Hox gene family playing a central role during axial body patterning and morphogenesis. DNA modification studies have shown that the function of Hox genes is partly governed by the methylation-medi...
Source: Gene Expression Patterns - May 24, 2017 Category: Genetics & Stem Cells Source Type: research
The expression of sialyltransferases is regulated by the bioavailability and biosynthesis of sialic acids
Publication date: January 2017 Source:Gene Expression Patterns, Volumes 23–24 Author(s): Kaya Bork, Wenke Weidemann, Beatrice Berneck, Magdalena Kuchta, Dorit Bennmann, Annett Thate, Otmar Huber, Vinayaga S. Gnanapragassam, Rüdiger Horstkorte Glycosylation is the most frequent and important post-translational modification of proteins. It occurs on specific consensus sequences but the final structure of a particular glycan is not coded on the DNA, rather it depends on the expression of the required enzymes and the availability of substrates (activated monosaccharides). Sialic acid (Sia) is the terminal monosac...
Source: Gene Expression Patterns - May 24, 2017 Category: Genetics & Stem Cells Source Type: research
Characterization of Sgo1 expression in developing and adult mouse
Publication date: November 2017 Source:Gene Expression Patterns, Volumes 25–26 Author(s): Andrew T. Song, Antonella Galli, Severine Leclerc, Stanley Nattel, Craig Mandato, Gregor Andelfinger SGO1 has been characterized in its function in correct cell division and its role in centrosome cohesion in the nucleus. However, its organ-specific maturation-related expression pattern in vivo remains largely uncharacterized. Here, we show clear SGO1 expression in post-developmental neuronal cells and cytoplasmic localisation in nucleated cells with a transgenic mice model and immunohistochemistry of wild type mice. We dem...
Source: Gene Expression Patterns - May 24, 2017 Category: Genetics & Stem Cells Source Type: research
Embryonic expression of festina lente (fel), a novel maternal gene, in the oligochaete annelid Tubifex tubifex
Publication date: November 2017 Source:Gene Expression Patterns, Volumes 25–26 Author(s): Takuma Nakamura, Inori Shiomi, Takashi Shimizu We have cloned and characterized the expression of a novel maternal gene festina lente (designated Ttu-fel) from the clitellate annelid Tubifex tubifex. Northern blot analyses have shown that Ttu-fel mRNA is approximately 8 kbp in length and that its expression is restricted to oocytes undergoing maturation division and early embryos up to 22-cell stage. Maternal transcripts of Ttu-fel are first detected in oocytes in the ovary of young adults (ca. 40 days after hatching); its exp...
Source: Gene Expression Patterns - May 24, 2017 Category: Genetics & Stem Cells Source Type: research
Dorsal fin development in flounder, Paralichthys olivaceus: Bud formation and its cellular origin
Publication date: November 2017 Source:Gene Expression Patterns, Volumes 25–26 Author(s): Jie Chen, Xiaoyu Liu, Xiaohua Yao, Fei Gao, Baolong Bao The development of the median fin has not been investigated extensively in teleosts, although in other fishes it has been proposed that it involves the same genetic programs operating in the paired appendages. Adult median fins develop from the larval bud; therefore an investigation of fin bud formation and its cellular origin is essential to understanding the maturation mechanisms. In Paralichthys olivaceus, skeletogenesis proceeds from an anterior to posterior directio...
Source: Gene Expression Patterns - May 24, 2017 Category: Genetics & Stem Cells Source Type: research
sept8a and sept8b mRNA expression in the developing and adult zebrafish
Publication date: November 2017 Source:Gene Expression Patterns, Volumes 25–26 Author(s): Constantin Berger, Frederik Helmprobst, Prisca Chapouton, Christina Lillesaar, Christian Stigloher Septins are highly conserved GTP-binding proteins involved in numerous cellular processes. Despite a growing awareness of their roles in the cell biology, development and signal transmission in nervous systems, comparably little is known about precise septin expression. Here, we use the well-established model organism zebrafish (Danio rerio) to unravel the expression of sept8a and sept8b, with special focus on the CNS. We perfor...
Source: Gene Expression Patterns - May 24, 2017 Category: Genetics & Stem Cells Source Type: research
