Expression of Prolyl hydroxylases 2 and 3 in chick embryos

Publication date: Available online 8 April 2016 Source:Gene Expression Patterns Author(s): Jake Barber, Nobue Itasaki Hypoxic cellular response is crucial for normal development as well as in pathological conditions in order to tolerate low oxygen. The response is mediated by Hypoxia Inducible Factors (HIFs), where the α-subunit of HIF is stabilised and able to function only in low oxygen. Prolyl hydroxyrases (PHDs) are oxygen dependent dioxygenase enzymes that hydroxyrate HIF-α leading to HIF degradation. Thus PHDs function as an oxygen sensor for the function of HIFs. Here we describe the mRNA expression pattern of PHDs in chick embryos. Up to embryonic day 2, PHDs are weak without specific localisation, whereas from day 3 localised expression was observed in the eye, branchial arches and dermomyotome. Later in the limb development PHDs were expressed in the perichondral mesenchyme, excluded from the developing limb cartilages.

Source: Gene Expression Patterns - April 7, 2016 Category: Genetics & Stem Cells Source Type: research