Bis(monoacylglycero)phosphate as a Macrophage Enriched Phospholipid
Abstract
Bis(monoacylglycero)phosphate (BMP) is a structural isomer of phosphatidylglycerol (PtdGro) with an unusual sn-1:sn-1′ fatty acyl configuration and is found almost exclusively in late endosomes/lysosomes. BMP comprises only about 1–2 % of the total phospholipids in most mammalian cells, but accumulates in tissues of humans and animals with lysosomal storage disorders including the gangliosidoses. Total BMP content was significantly greater in cells of macrophage/microglial origin than in cells of macroglial origin. BMP composition was similar in tumorigenic/metastatic macrophages and non-tum...
Source: Lipids - July 24, 2015 Category: Lipidology Source Type: research
Chemical Synthesis of the Epimeric (23 R )- and (23 S )-Fluoro Derivatives of Bile Acids via Horner–Wadsworth–Emmons Reaction
Abstract
A method for the synthesis of two (23R)- and (23S)-epimeric pairs of 23-fluoro-3α,7α,12α-trihydroxy-5β-cholan-24-oic acid and 23-fluoro-3α,7α-dihydroxy-5β-cholan-24-oic acid is described. The key intermediates, 23,24-dinor-22-aldehyde peracetates were prepared from cholic and chenodeoxycholic acids via the 24-nor-22-ene, 24-nor-22ξ,23-epoxy, and 23,24-dinor-22-aldehyde derivatives. The Horner–Wadsworth–Emmons reaction of the 23,24-dinor-22-aldehydes using triethyl 2-fluoro-2-phosphonoacetate in the presence of LiCl and 1,8-diazabicyclo[5,4,0]undec-7-ene (DBU), and subsequent hydrogena...
Source: Lipids - July 21, 2015 Category: Lipidology Source Type: research
Characterization of Wax Esters by Electrospray Ionization Tandem Mass Spectrometry: Double Bond Effect and Unusual Product Ions
Abstract
A series of different types of wax esters (represented by RCOOR′) were systematically studied by using electrospray ionization (ESI) collision-induced dissociation tandem mass spectrometry (MS/MS) along with pseudo MS3 (in-source dissociation combined with MS/MS) on a quadrupole time-of-flight (Q-TOF) mass spectrometer. The tandem mass spectra patterns resulting from dissociation of ammonium/proton adducts of these wax esters were influenced by the wax ester type and the collision energy applied. The product ions [RCOOH2]+, [RCO]+ and [RCO−H2O]+ that have been reported previously were detecte...
Source: Lipids - July 16, 2015 Category: Lipidology Source Type: research
Hypercholesterolemia Induced Immune Response and Inflammation on Progression of Atherosclerosis in Apob tm2Sgy Ldlr tm1Her /J Mice
Abstract
The effect of hypercholesterolemia induced immune response and inflammation on progression of atherosclerosis in ApoB
tm25gy
LDLr
tm1Her mice, expressing only ApoB100 and deficient in the low density lipoprotein (LDL) receptor, thus closely resembling human cholesterol transport is not well defined. Atherosclerosis was induced by a high cholesterol diet and its progression was studied at 8, 14 and 20 weeks. Antibody response was determined by ELISA. Lymphocytes in spleen and aortic expression of inflammatory markers...
Source: Lipids - July 16, 2015 Category: Lipidology Source Type: research
Lower Concentration of n-3 in the Red Blood Cells and Plasma of Lambs when their Dams were Fed a Diet High Compared with Low in n-6 Fatty Acids at Joining
Abstract
Feeding ewes a diet high in n-6 in late gestation can affect fatty acid concentrations in the newborn lamb. The effect of feeding ewes a high n-6 diet prior to conception and in early gestation on lamb n-6 and n-3 status has not previously been examined. The aim of the current study was to determine whether the concentration of n-6 was higher and n-3 was lower in lamb red blood cells (RBC) and plasma when Merino dams were fed a diet high in n-6 either pre-conception only or both pre-conception and in early gestation. Dams were fed a diet low (silage) or high (oats/CSM) in n-6 for either 6 weeks ...
Source: Lipids - July 16, 2015 Category: Lipidology Source Type: research
