Berberine Inhibits Oxygen Consumption Rate Independent of Alteration in Cardiolipin Levels in H9c2 Cells
We examined if BBR inhibited oxygen consumption rate in H9c2 cardiac myocytes through alteration in Ptd2Gro metabolism. Treatment of H9c2 cells with BBR resulted in a rapid (within minutes) concentration-dependent decrease in the oxygen consumption rate (OCR) as determined using a Seahorse XF24 analyzer. Concentrations of BBR as low as 1  µM were effective in inhibiting OCR. In addition, all concentrations of BBR inhibited the fatty acid-mediated increase in OCR that was observed in untreated cells. Treatment of H9c2 cells with up to 25 µM BBR for 24 h markedly reduced [3H]thymidine incorporation into cells but did no...
Source: Lipids - November 1, 2017 Category: Lipidology Source Type: research
Relationship Between Circulating Fatty Acids and Fatty Acid Ethanolamide Levels After a Single 2-h Dietary Fat Feeding in Male Sprague –Dawley Rats
AbstractPrevious studies show that long term variations in dietary fat consumption impact circulating fatty acid ethanolamide (FAE) concentrations, however, few studies have investigated short term effects of dietary fat feeding on FAE levels. The  trial’s objective was to explore the effect of acute feeding of varying amounts of dietary n-9 and n-3 fatty acids on plasma and organ levels of FAE. Sixty-four rats were assigned to four groups fed meals containing 40% of energy as either safflower oil (control), canola oil (CO), or DHA rich oi l (DRO), each consumed as a bolus within a 2-h window. Plasma and tissue FAE leve...
Source: Lipids - November 1, 2017 Category: Lipidology Source Type: research
PAM, OLA, and LNA are Differentially Taken Up and Trafficked Via Different Metabolic Pathways in Porcine Adipocytes
AbstractDietary fatty acids have different effects on fat deposition in pigs. To clarify the underlying mechanisms of this difference, we compared the metabolism of palmitic (PAM, saturated), oleic (OLA, monounsaturated) and linoleic acid (LNA, polyunsaturated) in porcine adipocytes treated with 100  μM PAM, OLA or LNA. We observed that the adipocytes incubated with LNA accumulated more lipids compared with those treated with PAM and OLA. We then probed the metabolism of these fatty acids in porcine adipocytes by using isotope-labelled fatty acids. The results showed that 42% of the [1-14C] LNA, 34% of the [1-14C] PAM an...
Source: Lipids - November 1, 2017 Category: Lipidology Source Type: research
Flaxseed Oil Supplementation Improve Gene Expression Levels of PPAR- γ, LP(a), IL-1 and TNF-α in Type 2 Diabetic Patients with Coronary Heart Disease
This study was carried out to determine the effects of flaxseed oil administration on gene expression levels related to insulin, lipid and inflammation in overweight diabetic patients with coronary heart disease (CHD). This randomized double-blind, placebo-controlled trial was conducted among 60 diabetic patients with CHD. Subjects were randomly allocated into two groups to intake either 1000  mg n-3 fatty acid from flaxseed oil containing 400 mg α-Linolenic acid [ALA (18:3n-3)] (n = 30) or placebo (n = 30) twice a day for 12 weeks. Gene expression related to insulin, lipid and inflammation were quantified in perip...
Source: Lipids - November 1, 2017 Category: Lipidology Source Type: research
Tissue Uptake Mechanisms Involved in the Clearance of Non-Protein Nanoparticles that Mimic LDL Composition: A Study with Knockout and Transgenic Mice
In conclusion, results support LDLR and apoE as the key players for LDE clearance, apoA-I also infl uences those processes. (Source: Lipids)
Source: Lipids - November 1, 2017 Category: Lipidology Source Type: research
Sex Differences in Blood HDL-c, the Total Cholesterol/HDL-c Ratio, and Palmitoleic Acid are Not Associated with Variants in Common Candidate Genes
AbstractBlood lipids are associated with cardiovascular disease (CVD) risk. Moreover, circulating lipid and fatty acid levels vary between men and women, and evidence demonstrates these traits may be influenced by single nucleotide polymorphisms (SNP). Sex-genotype interactions related to blood lipids and fatty acids have been poorly investigated and may help elucidate sex differences in CVD risk. The goal of this study was to investigate if the influence of SNPs previously associated with blood lipids and fatty acids varies in a sex-specific manner. Lipids and fatty acids were measured in serum and red blood cells (RBC), ...
Source: Lipids - October 27, 2017 Category: Lipidology Source Type: research
Chronic Exercise Reduces CETP and Mesterolone Treatment Counteracts Exercise Benefits on Plasma Lipoproteins Profile: Studies in Transgenic Mice
AbstractRegular exercise and anabolic androgenic steroids have opposing effects on the plasma lipoprotein profile and risk of cardio-metabolic diseases in humans. Studies in humans and animal models show conflicting results. Here, we used a mice model genetically modified to mimic human lipoprotein profile and metabolism. They under-express the endogenous LDL receptor gene (R1) and express a human transgene encoding the cholesteryl ester transfer protein (CETP), normally absent in mice. The present study was designed to evaluate the independent and interactive effects of testosterone supplementation, exercise training and ...
Source: Lipids - October 20, 2017 Category: Lipidology Source Type: research
PAM, OLA, and LNA are Differentially Taken Up and Trafficked Via Different Metabolic Pathways in Porcine Adipocytes
AbstractDietary fatty acids have different effects on fat deposition in pigs. To clarify the underlying mechanisms of this difference, we compared the metabolism of palmitic (PAM, saturated), oleic (OLA, monounsaturated) and linoleic acid (LNA, polyunsaturated) in porcine adipocytes treated with 100  μM PAM, OLA or LNA. We observed that the adipocytes incubated with LNA accumulated more lipids compared with those treated with PAM and OLA. We then probed the metabolism of these fatty acids in porcine adipocytes by using isotope-labelled fatty acids. The results showed that 42% of the [1-14C] LNA, 34% of the [1-14C] PAM an...
Source: Lipids - October 20, 2017 Category: Lipidology Source Type: research
Relationship Between Circulating Fatty Acids and Fatty Acid Ethanolamide Levels After a Single 2-h Dietary Fat Feeding in Male Sprague –Dawley Rats
AbstractPrevious studies show that long term variations in dietary fat consumption impact circulating fatty acid ethanolamide (FAE) concentrations, however, few studies have investigated short term effects of dietary fat feeding on FAE levels. The  trial’s objective was to explore the effect of acute feeding of varying amounts of dietary n-9 and n-3 fatty acids on plasma and organ levels of FAE. Sixty-four rats were assigned to four groups fed meals containing 40% of energy as either safflower oil (control), canola oil (CO), or DHA rich oi l (DRO), each consumed as a bolus within a 2-h window. Plasma and tissue FAE leve...
Source: Lipids - October 14, 2017 Category: Lipidology Source Type: research
Erratum to: Inhibition of Angiotensin II-Induced Cardiac Fibrosis by Atorvastatin in Adiponectin Knockout Mice
In the original published article, Victor Serebruany was designated as the corresponding author in error. The corresponding author is Jong Sung Park. Dr. Park can be reached by email at thinkmed@dau.ac.kr. (Source: Lipids)
Source: Lipids - October 13, 2017 Category: Lipidology Source Type: research
Mechanism of Phospholipid Hydrolysis for Oyster Crassostrea plicatula Phospholipids During Storage Using Shotgun Lipidomics
AbstractA fast and efficient shotgun lipidomics strategy was applied to analyze phospholipids (PL) in the oysterCrassostrea plicatula, including 29 species of phosphatidylcholine (PtdCho), 23 species of phosphatidylethanolamine (PtdEtn), 11 species of phosphatidylserine (PtdSer), 6 species of phosphatidylinositol (PtdIns), and 17 species of lysophospholipids (Lyso-PL). During storage at 4  °C for 7 days, the PL content decreased by 68.08%, but a significant increase in the FFA content was observed (from 63.11 to 318.72 μg/g). PtdCho and PtdIns decreased relatively by 64.97 and 67.49%, and PtdSer decreased most markedl...
Source: Lipids - October 3, 2017 Category: Lipidology Source Type: research
Fish Oil Finishing Diet Maintains Optimal n-3 Long-Chain Fatty Acid Content in European Whitefish (Coregonus lavaretus)
This study examined the effect of substituting vegetable oil for fish oil in feed, with subsequent re-introduction of fish oil-rich feed (finishing feeding) in late stages of growth, on the fatty acids of cultivated European whitefish (Coregonus lavaretus). Restorative finishing feeding with fish oil-rich feed for 15 and 25 Â weeks was sufficient to change the total content of nutritionally valuable long-chain n-3 fatty acids, eicosapentaenoic acid (20:5n-3) and docosahexaenoic acid (22:6n-3), to correspond to that of fish fed the fish oil-rich feed throughout their lifespan. Under natural conditions, 15 and 25Â weeks corr...
Source: Lipids - October 1, 2017 Category: Lipidology Source Type: research
The Phospholipid Composition of Kangaroo Spermatozoa Verified by Mass Spectrometric Lipid Analysis
AbstractCryopreservation of kangaroo sperm has not been successful so far, and yet there is no promising cryopreservation protocol for these cells available. However, conservation of gametes is extremely important, particularly in the context of preserving endangered species. As spermatozoa are comprised of different membrane systems, the composition of these membranes might account for difficulties in cryopreservation. Lipids, as the main components, affect the physical properties of biological membranes and play a major role in sperm maturation. Therefore, knowledge of the lipid composition is crucial for any further ste...
Source: Lipids - October 1, 2017 Category: Lipidology Source Type: research
Fish Oil Finishing Diet Maintains Optimal n-3 Long-Chain Fatty Acid Content in European Whitefish ( Coregonus lavaretus )
This study examined the effect of substituting vegetable oil for fish oil in feed, with subsequent re-introduction of fish oil-rich feed (finishing feeding) in late stages of growth, on the fatty acids of cultivated European whitefish (Coregonus lavaretus). Restorative finishing feeding with fish oil-rich feed for 15 and 25 Â weeks was sufficient to change the total content of nutritionally valuable long-chain n-3 fatty acids, eicosapentaenoic acid (20:5n-3) and docosahexaenoic acid (22:6n-3), to correspond to that of fish fed the fish oil-rich feed throughout their lifespan. Under natural conditions, 15 and 25Â weeks corr...
Source: Lipids - October 1, 2017 Category: Lipidology Source Type: research
Fatty Acid Composition and Contents of Seven Commercial Fish Species of Genus Coregonus from Russian Subarctic Water Bodies
AbstractIn several Russian northern lakes and rivers, Arctic ciscoCoregonus autumnalis, least ciscoC. sardinella, peledC. peled, tugunC. tugun, broad whitefishC. nasus, whitefishC. lavaretus and vendaceC. albula were sampled in periods of officially permitted commercial fishery. Special attention was paid to contents (mg  g−1 of wet weight) of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in muscle tissues (filets), which are essential for human nutrition. The highest values of EPA  + DHA content in semi-anadromous fish and freshwater fish were recorded forC. autumnalis from the Yenisei River, 17.60  mgÂ...
Source: Lipids - September 30, 2017 Category: Lipidology Source Type: research
