Journal of Histochemistry and Cytochemistry This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Improving the State of the Science of Immunohistochemistry: The Histochemical Society's Standards of Practice
(Source: Journal of Histochemistry and Cytochemistry)
Source: Journal of Histochemistry and Cytochemistry - September 25, 2014 Category: Biochemistry Authors: Baskin, D. G., Hewitt, S. M. Tags: Guest Editorial Source Type: research

Characterization of a Novel Recombinant Hyaluronan Binding Protein for Tissue Hyaluronan Detection
Tumor necrosis factor-Stimulated Gene 6 protein (TSG-6) is a hyaluronan (HA)-binding glycoprotein containing an HA-binding Link module. Because of its well-defined structure, HA binding properties and small size, TSG-6 is an excellent candidate as an alternative to animal-derived HA-binding protein (HABP) for the detection of HA. The present work describes the generation and characterization of a novel recombinant HA-binding probe obtained by fusion of a modified TSG-6 Link module with mutationally inactivated heparin-binding sequence and the Fc portion of human IgG1 (TSG-6-Hep-Fc) for tissue HA detection in histological s...
Source: Journal of Histochemistry and Cytochemistry - August 25, 2014 Category: Biochemistry Authors: Jadin, L., Huang, L., Wei, G., Zhao, Q., Gelb, A. B., Frost, G. I., Jiang, P., Shepard, H. M. Tags: Articles Source Type: research

Revisiting Fixation and Embedding Techniques for Optimal Detection of Dendritic Cell Subsets in Tissues
Organ-specific cell types are maintained by tissue homeostasis and may vary in nature and/or frequency in pathological situations. Moreover, within a cell lineage, some sub-populations, defined by combinations of cell-surface markers, may have specific functions. Dendritic cells are the epitome of such a population as they may be subdivided into discrete sub-groups with defined functions in specific compartments of various organs. Technically, to study the distribution of DC sub-populations, it involves performing multiparametric immunofluorescence on well-conserved organ structures. However, immunodetection may be impacte...
Source: Journal of Histochemistry and Cytochemistry - August 25, 2014 Category: Biochemistry Authors: Accart, N., Sergi, F., Rooke, R. Tags: Articles Source Type: research

Increased Immunoreactivity of Cathepsins in the Rat Esophagus under Chronic Acid Reflux Esophagitis
We have designed a stable rat chronic acid reflux esophagitis (RE) model. In gastrointestinal lesions, several lysosomal cathepsins are known to participate in epithelial permeability in cell-cell connections, such as tight junctions in ulcerative colitis. However, very few studies have focused on the distribution of cathepsins in the esophageal multilayer squamous epithelium. Therefore to clarify the role of cathepsins in RE, we investigated their immunohistological localization in the esophageal epithelium under normal conditions and after RE. Of the cathepsins examined (cathepsins B, C, D, F, H, L, S, and X), granular i...
Source: Journal of Histochemistry and Cytochemistry - August 25, 2014 Category: Biochemistry Authors: Suyama, M., Koike, M., Asaoka, D., Mori, H., Oguro, M., Ueno, T., Nagahara, A., Watanabe, S., Uchiyama, Y. Tags: Articles Source Type: research

CEACAM2-L on Spermatids Interacts with Poliovirus Receptor on Sertoli Cells in Mouse Seminiferous Epithelium
In this study, we investigated the interaction between CEACAM2-L and Poliovirus receptor (PVR), which is also from the IgSF and is expressed by Sertoli cells. Immunohistochemical analysis showed that CEACAM2-L expressed on elongated spermatids was in close contact with PVR-positive cell processes of Sertoli cells. Immunoprecipitation experiments both in vivo and in vitro demonstrated a direct heterophilic interaction between CEACAM2-L and PVR. We show that the N-terminal Ig domain of CEACAM2-L was critical for its interaction with PVR. In addition, we found that CEACAM2-L formed heterophilic trans-tetramers with PVR in tra...
Source: Journal of Histochemistry and Cytochemistry - August 25, 2014 Category: Biochemistry Authors: Salaheldeen, E., Howida, A., Wakayama, T., Iida, H. Tags: Articles Source Type: research

Immunohistological Insight into the Correlation between Neuropilin-1 and Epithelial-Mesenchymal Transition Markers in Epithelial Ovarian Cancer
This study is the first to demonstrate the relationship between NRP-1 expression and EMT markers vimentin, N-cadherin, E-cadherin and Slug. We used tissue microarrays containing the three main subtypes of EOC tumors: serous, mucinous cystadenocarcinoma and endometrioid adenocarcinoma and representative cases retrieved from our pathology archives. Immunohistochemistry was performed to detect the expression levels and location of NRP-1 and the aforementioned EMT proteins. NRP-1 was mainly expressed on cancer cells but not in normal ovarian surface epithelium (OSE). The Immunoreactive Scoring (IRS) values revealed that the ex...
Source: Journal of Histochemistry and Cytochemistry - August 25, 2014 Category: Biochemistry Authors: Adham, S. A. I., Al Harrasi, I., Al Haddabi, I., Al Rashdi, A., Al Sinawi, S., Al Maniri, A., Ba-Omar, T., Coomber, B. L. Tags: Articles Source Type: research

Immunolocalization of Water Channel Proteins AQP1 and AQP4 in Rat Spinal Cord
Aquaporin (AQP) is a water-selective channel protein. In the brain, AQPs play critical roles in the production of cerebrospinal fluid and in edema formation. In contrast, the expression and role of AQPs in spinal cord are unclear. We aimed to investigate the localization of AQP1 and AQP4 in normal rat spinal cord compared with the expression of marker proteins for astrocytes, neurons, and endothelial cells. Immunohistochemistry demonstrated that AQP1 and AQP4 are expressed along all levels of the spinal cord from the cervical to lumbar levels. AQP1 immunolabeling was observed in the dorsal horns in the gray matter, whereas...
Source: Journal of Histochemistry and Cytochemistry - July 25, 2014 Category: Biochemistry Authors: Oklinski, M. K., Lim, J.-S., Choi, H.-J., Oklinska, P., Skowronski, M. T., Kwon, T.-H. Tags: Articles Source Type: research

Heat-induced Antigen Retrieval in Conventionally Processed Epon-embedded Specimens: Procedures and Mechanisms
We studied the effectiveness of heat-induced antigen retrieval (HIAR) in conventionally processed, epon-embedded specimens and the mechanisms of HIAR in the specimens. Frozen sections were first immunostained to examine the possibility of using HIAR for 18 antigens to avoid the effects of epoxy resin embedment. The antigenicity of 7 out of 18 antigens was retrieved with glutaraldehyde fixation followed by osmium tetroxide treatment whereas none were retrieved with glutaraldehyde fixation without post-osmication. Six antigens also exhibited positive immunostaining in semi-thin epon sections when the sections were deplastici...
Source: Journal of Histochemistry and Cytochemistry - July 25, 2014 Category: Biochemistry Authors: Yamashita, S., Okada, Y. Tags: Articles Source Type: research

Efficiency of the Polyethylene-Glycol (PEG) Embedding Medium for Plant Histochemistry
Histochemical analyses in plants are commonly performed on hand-made sections of fresh materials. The disadvantages of embedding in historesin, paraffin or paraplast® are the alterations to cellular contents, the high costs and few evident results, depending on the test. Polyethylene-glycol (PEG), as a low cost, hydrophilic medium that maintains most of the cellular features similar to fresh conditions, may be useful for obtaining good histochemical results in thinner and homogeneous sections. The current study aimed to compare the efficiency of PEG as an embedding medium for histochemical analyses of primary and secon...
Source: Journal of Histochemistry and Cytochemistry - July 25, 2014 Category: Biochemistry Authors: Ferreira, B. G., Teixeira, C. T., Isaias, R. M. S. Tags: Articles Source Type: research

Better Osteoporotic Fracture Healing with Sintered Dicalcium Pyrophosphate (SDCP) Treatment: A Rat Femoral Fracture Model
The aim of this study was to evaluate the effect of sintered dicalcium pyrophosphate (SDCP) on fracture healing in an osteoporotic rat model. Female Sprague-Dawley rats (8 weeks old) were randomly allocated into five groups: sham-operated group, and bilateral ovariectomized group treated with SDCP, alendronate, calcitonin, or no treatment. Rats were sacrificed at 6 or 16 weeks after fracture. Fracture sites were examined by microcomputed tomography (microCT), histology, and mechanical testing. The results showed that SDCP mildly suppressed callus remodeling at 6 weeks, but not at 16 weeks. The lamellar bone in the callus a...
Source: Journal of Histochemistry and Cytochemistry - July 25, 2014 Category: Biochemistry Authors: Kuo, Y.-J., Sun, J.-S., Rau, G., Chen, C.-H., Tsai, T.-H., Tsuang, Y.-H. Tags: Articles Source Type: research

Histological Evidence of Increased Osteoclast Cell Number and Asymmetric Bone Resorption Activity in the Tibiae of Interleukin-6-Deficient Mice
Interleukin-6 (IL-6) is a multifunctional cytokine considered to modulate bone homeostasis. Based on previous contradictory studies, we aimed to verify the influence of IL-6 deficiency on bone remodeling using an IL-6 knockout (IL-6-/-) murine model. Eight-month-old male mice, homozygous for the disrupted IL-6 gene, and their wild type (WT) littermates (control), were used. After transcardiac perfusion, tibiae were removed for histochemical analysis. Compared with the control group, IL-6 deficiency increased tartrate resistant acid phosphatase (TRAP)-positive osteoclast numbers and up-regulated the alkaline phosphatase (AL...
Source: Journal of Histochemistry and Cytochemistry - July 25, 2014 Category: Biochemistry Authors: Liu, H., Feng, W., Yimin, , Cui, J., Lv, S., Hasegawa, T., Sun, B., Li, J., Oda, K., Amizuka, N., Li, M. Tags: Articles Source Type: research

Pretreatment MicroRNA Level and Outcome in Sorafenib-treated Hepatocellular Carcinoma
Sorafenib represents the first effective targeted therapy for advanced stage hepatocellular carcinoma (HCC); however, adequate patient stratification regarding sorafenib-responsiveness is still missing. Our aim was to analyse the association between the pretreatment microRNA profile of HCC and patient survival under sorafenib treatment. Total RNA was extracted from diagnostic fine-needle aspiration biopsy (FNAB) cytological smears of 20 advanced stage HCC patients collected between June 2008 and July 2012. All patients underwent sorafenib administration after FNA. Clinicopathological and survival data were recorded. Fourte...
Source: Journal of Histochemistry and Cytochemistry - July 25, 2014 Category: Biochemistry Authors: Gyongyosi, B., Vegh, E., Jaray, B., Szekely, E., Fassan, M., Bodoky, G., Schaff, Z., Kiss, A. Tags: Articles Source Type: research

Noggin Expression in the Adult Retina Suggests a Conserved Role during Vertebrate Evolution
Vertebrates share common mechanisms in the control of development and in the maintenance of neural and retinal function. The secreted factor Noggin, a BMP inhibitor, plays a crucial role in neural induction during embryonic development. Moreover, we have shown its involvement in retinal differentiation of pluripotent cells. Here we show Noggin expression in the adult retina in three vertebrate species. Four Noggin genes are present in zebrafish (Danio rerio; ZbNog1, 2, 3, 5), three in frog (Xenopus laevis; XenNog1, 2 and 4), and one in mouse (Mus musculus; mNog). Quantitative RT-PCR experiments show the presence of ZbNog3 ...
Source: Journal of Histochemistry and Cytochemistry - June 25, 2014 Category: Biochemistry Authors: Messina, A., Incitti, T., Bozza, A., Bozzi, Y., Casarosa, S. Tags: Articles Source Type: research

Elution of High-affinity (>10-9 KD) Antibodies from Tissue Sections: Clues to the Molecular Mechanism and Use in Sequential Immunostaining
Inconsistent results obtained with published methods for the elution of antibodies from tissue sections prompted the assessment of both old and new methods in combination with monoclonal rabbit antibodies of known, increased affinity (above 1x10-9 KD). We tested an acidic (pH 2) glycine buffer, a 6 M urea hot buffer and a 2-Mercaptoethanol, SDS buffer (2-ME/SDS). Some antibodies were not removed by the glycine pH 2 or 6 M urea hot buffers, indicating that antibodies survive much harsher conditions than previously believed. We found that the elution is dependent upon the antibody affinity and is reduced by species-specific ...
Source: Journal of Histochemistry and Cytochemistry - June 25, 2014 Category: Biochemistry Authors: Gendusa, R., Scalia, C. R., Buscone, S., Cattoretti, G. Tags: Articles Source Type: research

Intrauterine Growth Restriction Impairs Small Intestinal Mucosal Immunity in Neonatal Piglets
In conclusion, IUGR was shown to impair the mucosal immunity of the SI in neonatal piglets, and the ileum was the major site of impairment. (Source: Journal of Histochemistry and Cytochemistry)
Source: Journal of Histochemistry and Cytochemistry - June 25, 2014 Category: Biochemistry Authors: Dong, L., Zhong, X., Ahmad, H., Li, W., Wang, Y., Zhang, L., Wang, T. Tags: Articles Source Type: research