Journal of Histochemistry and Cytochemistry This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Leucine Zipper Tumor Suppressor 2 Inhibits Cell Proliferation and Regulates Lef/Tcf-dependent Transcription through Akt/GSK3{beta} Signaling Pathway in Lung Cancer
Leucine zipper tumor suppressor 2 (LZTS2) is implicated in several cancers; however, its biological mechanisms in non-small cell lung cancer (NSCLC) are not yet understood. We found that low levels of LZTS2 in NSCLC were correlated with tumor and nodal status. LZTS2 could inhibit cell proliferation and cell cycle transition at the G1/S phase and was implicated in the regulation of proteins associated with the canonical Wnt pathway, including GSK3β and β-catenin through inactivating the Akt pathway. These results provide novel mechanistic insight into the biological roles of LZTS2 in lung cancer cells. (Source: Jo...
Source: Journal of Histochemistry and Cytochemistry - August 26, 2013 Category: Biochemistry Authors: Cui, Q.-Z., Tang, Z.-P., Zhang, X.-P., Zhao, H.-Y., Dong, Q.-Z., Xu, K., Wang, E.-H. Tags: Articles Source Type: research

GLP-1 Receptor Is Expressed in Human Stomach Mucosa: Analysis of Its Cellular Association and Distribution within Gastric Glands
This study provides the first description of GLP-1R expression in human gastric glands and its specific cellular association. Our data suggest that GLP-1 may act directly on the gastric mucosa to modulate its complex functions. (Source: Journal of Histochemistry and Cytochemistry)
Source: Journal of Histochemistry and Cytochemistry - August 26, 2013 Category: Biochemistry Authors: Broide, E., Bloch, O., Ben-Yehudah, G., Cantrell, D., Shirin, H., Rapoport, M. J. Tags: Articles Source Type: research

Diagnostic Value of Immunohistochemical Staining of GP73, GPC3, DCP, CD34, CD31, and Reticulin Staining in Hepatocellular Carcinoma
This study aimed to evaluate a panel of immunostaining markers (including GP73, GPC3, DCP, CD34, and CD31) as well as reticulin staining to distinguish HCC from the mimickers. Our results revealed that CD34 immunostaining and reticulin staining were highly sensitive for the diagnosis of HCC. A special immunoreaction pattern of GP73—a diffuse coarse-block pattern in a perinuclear region or a concentrated cluster-like or cord-like pattern in a certain part of the cytoplasm—was observed in HCC cells, in contrast to the cytoplasmic fine-granular pattern in surrounding non-tumor cells and non-malignant nodules. This...
Source: Journal of Histochemistry and Cytochemistry - August 26, 2013 Category: Biochemistry Authors: Yao, S., Zhang, J., Chen, H., Sheng, Y., Zhang, X., Liu, Z., Zhang, C. Tags: Articles Source Type: research

Reduced FHIT Expression Is Associated with Mismatch Repair Deficient and High CpG Island Methylator Phenotype Colorectal Cancer
Colorectal cancer (CRC) is a heterogeneous disease and a major contributor to world cancer mortality rates. Molecular subtypes of CRC have become standards for CRC classification and have established prognostic potential. Here, we attempt to corroborate and provide further insight pertinent to the fragile histidine triad (FHIT) gene in microsatellite instable (MSI), microsatellite stable (MSS), and CpG island methylator phenotype (CIMP) CRC subtypes. We employed array comparative genomic hybridization and multiplex ligation-dependent probe amplification (MLPA) techniques to survey genomic aberrations in FHIT gene and their...
Source: Journal of Histochemistry and Cytochemistry - August 26, 2013 Category: Biochemistry Authors: Al-Temaimi, R. A., Jacob, S., Al-Ali, W., Thomas, D. A., Al-Mulla, F. Tags: Articles Source Type: research

Reduced Sulfation of Chondroitin Sulfate but Not Heparan Sulfate in Kidneys of Diabetic db/db Mice
Heparan sulfate proteoglycans are hypothesized to contribute to the filtration barrier in kidney glomeruli and the glycocalyx of endothelial cells. To investigate potential changes in proteoglycans in diabetic kidney, we isolated glycosaminoglycans from kidney cortex from healthy db/+ and diabetic db/db mice. Disaccharide analysis of chondroitin sulfate revealed a significant decrease in the 4-O-sulfated disaccharides (D0a4) from 65% to 40%, whereas 6-O-sulfated disaccharides (D0a6) were reduced from 11% to 6%, with a corresponding increase in unsulfated disaccharides. In contrast, no structural differences were observed i...
Source: Journal of Histochemistry and Cytochemistry - July 25, 2013 Category: Biochemistry Authors: Reine, T. M., Grondahl, F., Jenssen, T. G., Hadler-Olsen, E., Prydz, K., Kolset, S. O. Tags: Articles Source Type: research

Melatonin Ameliorates Injury and Specific Responses of Ischemic Striatal Neurons in Rats
This study aimed to examine the morphological changes of different striatal neuron types and the effect of melatonin on striatal injury by MCAO. The results showed that MCAO induced striatum-related dysfunctions of locomotion, coordination, and cognition, which were remarkably relieved with melatonin treatment. MCAO induced severe striatal neuronal apoptosis and loss, which was significantly decreased with melatonin treatment. Within the outer zone of the infarct, the number of Darpp-32+ projection neurons and the densities of dopamine-receptor-1 (D1)+ and dopamine-receptor-2 (D2)+ fibers were reduced; however, both parval...
Source: Journal of Histochemistry and Cytochemistry - July 25, 2013 Category: Biochemistry Authors: Ma, Y., Feng, Q., Ma, J., Feng, Z., Zhan, M., OuYang, L., Mu, S., Liu, B., Jiang, Z., Jia, Y., Li, Y., Lei, W. Tags: Articles Source Type: research

Lymphatic Endothelial Differentiation in Pulmonary Lymphangioleiomyomatosis Cells
This study has implications for the histological diagnosis of LAM, the origin of the neoplastic cells, and potential future treatment with drugs targeting lymphangiogenesis. (Source: Journal of Histochemistry and Cytochemistry)
Source: Journal of Histochemistry and Cytochemistry - July 25, 2013 Category: Biochemistry Authors: Davis, J. M., Hyjek, E., Husain, A. N., Shen, L., Jones, J., Schuger, L. A. Tags: Articles Source Type: research

Morphometry Analysis of Lymphatics in Pulmonary Adenocarcinomas With a Lepidic Growth Pattern
Lymph vessels play an important role in tumor progression. Pulmonary adenocarcinomas, accounting for half of non-small-cell lung carcinomas, compose a spectrum of histological types, exclusively or without a lepidic growth pattern (LGP) along preserved interalveolar septa. In that context, this study was designed to investigate the lymphatic vascular pattern associated with LGP and the concomitant invasive component of pulmonary adenocarcinomas. Using the D2-40 monoclonal antibody as a marker of lymphatic endothelial cells, the lymphatic vessel density (LVD) and vessel-area fraction (LVAF) were morphometrically analyzed in...
Source: Journal of Histochemistry and Cytochemistry - July 25, 2013 Category: Biochemistry Authors: Sannier, A., Kambouchner, M., Danel, C., Callard, P., Bernaudin, J.-F. Tags: Articles Source Type: research

Systematic Characterization of Myocardial Inflammation, Repair, and Remodeling in a Mouse Model of Reperfused Myocardial Infarction
Mouse models of myocardial infarction are essential tools for the study of cardiac injury, repair, and remodeling. Our current investigation establishes a systematic approach for quantitative evaluation of the inflammatory and reparative response, cardiac function, and geometry in a mouse model of reperfused myocardial infarction. Reperfused mouse infarcts exhibited marked induction of inflammatory cytokines that peaked after 6 hr of reperfusion. In the infarcted heart, scar contraction and chamber dilation continued for at least 28 days after reperfusion; infarct maturation was associated with marked thinning of the scar,...
Source: Journal of Histochemistry and Cytochemistry - July 25, 2013 Category: Biochemistry Authors: Christia, P., Bujak, M., Gonzalez-Quesada, C., Chen, W., Dobaczewski, M., Reddy, A., Frangogiannis, N. G. Tags: Articles Source Type: research

Bilateral Changes of Cannabinoid Receptor Type 2 Protein and mRNA in the Dorsal Root Ganglia of a Rat Neuropathic Pain Model
Cannabinoid receptor type 2 (CB2R) plays a critical role in nociception. In contrast to cannabinoid receptor type 1 ligands, CB2R agonists do not produce undesirable central nervous system effects and thus promise to treat neuropathic pain that is often resistant to medical therapy. In the study presented here, we evaluated the bilateral distribution of the CB2R protein and messenger RNA (mRNA) in rat dorsal root ganglia (DRG) after unilateral peripheral nerve injury using immunohistochemistry, western blot, and in situ hybridization analysis. Unilateral chronic constriction injury (CCI) of the sciatic nerve induced neurop...
Source: Journal of Histochemistry and Cytochemistry - June 25, 2013 Category: Biochemistry Authors: Svizenska, I. H., Brazda, V., Klusakova, I., Dubovy, P. Tags: Articles Source Type: research

Crlz-1 Is Prominently Expressed in Spermatogonia and Sertoli Cells during Early Testis Development and in Spermatids during Late Spermatogenesis
The expression of the Crlz-1 gene in mouse testis, where it was found to be expressed most highly among the tested mouse organs, was analyzed spatiotemporally by employing RT-PCR and in situ hybridization techniques with the aid of immunohistochemistry and/or immunofluorescence methods. In 1-week-old neonatal testis, Crlz-1 was strongly expressed in the spermatogonia and Sertoli cells in its seminiferous cord. In 2- to 3-week-old prepubertal testis, where Sertoli cells cease to proliferate, Crlz-1 expression dropped and remained weakly at the rim layer of seminiferous cords and/or tubules, where spermatogonia are present. ...
Source: Journal of Histochemistry and Cytochemistry - June 25, 2013 Category: Biochemistry Authors: Lim, J.-H., Choi, S.-Y., Yoo, H.-W., Cho, S.-J., Son, Y., Kang, C.-J. Tags: Articles Source Type: research

DEK Depletion Negatively Regulates Rho/ROCK/MLC Pathway in Non-Small Cell Lung Cancer
The human DEK proto-oncogene is a nuclear protein with suspected roles in human carcinogenesis. DEK appears to function in several nuclear processes, including transcriptional regulation and modulation of chromatin structure. To investigate the clinicopathological significance of DEK in patients with non–small cell lung cancer (NSCLC), we analyzed DEK immunohistochemistry in 112 NSCLC cases. The results showed that DEK was overexpressed mainly in the nuclear compartment of tumor cells. In squamous cell carcinoma, DEK-positive expression occurred in 47.9% (23/48) of cases, and in lung adenocarcinoma, DEK-positive expr...
Source: Journal of Histochemistry and Cytochemistry - June 25, 2013 Category: Biochemistry Authors: Wang, J., Sun, L., Yang, M., Luo, W., Gao, Y., Liu, Z., Qiu, X., Wang, E. Tags: Articles Source Type: research

4FISH-IF, a Four-Color Dual-Gene FISH Combined with p63 Immunofluorescence to Evaluate NKX3.1 and MYC Status in Prostate Cancer
NKX3.1 allelic loss and MYC amplification are common events during prostate cancer progression and have been recognized as potential prognostic factors in prostate cancer after radical prostatectomy or precision radiotherapy. We have developed a 4FISH-IF assay (a dual-gene fluorescence in situ hybridization combined with immunofluorescence) to measure both NKX3.1 and MYC status on the same slide. The 4FISH-IF assay contains four probes complementary to chromosome 8 centromere, 8p telomere, 8p21, and 8q24, as well as an antibody targeting the basal cell marker p63 visualized by immunofluorescence. The major advantages of th...
Source: Journal of Histochemistry and Cytochemistry - June 25, 2013 Category: Biochemistry Authors: Trudel, D., Zafarana, G., Sykes, J., Have, C. L., Bristow, R. G., van der Kwast, T. Tags: Articles Source Type: research

Diaphragm Muscle Remodeling in a Rat Model of Chronic Intermittent Hypoxia
Respiratory muscle remodeling occurs in human sleep apnea—a common respiratory disorder characterized by chronic intermittent hypoxia (CIH) due to recurrent apnea during sleep. We sought to determine if CIH causes remodeling in rat sternohyoid (upper airway dilator) and diaphragm muscles. Adult male Wistar rats were exposed to CIH (n=8), consisting of 90 sec of hypoxia (5% at the nadir; SaO2 ~80%)/90 sec of normoxia, 8 hr per day, for 7 consecutive days. Sham animals (n=8) were exposed to alternating air/air cycles in parallel. The effect of CIH on myosin heavy-chain (MHC) isoform (1, 2a, 2x, 2b) distribution, sarcop...
Source: Journal of Histochemistry and Cytochemistry - June 25, 2013 Category: Biochemistry Authors: Shortt, C. M., Fredsted, A., Bradford, A., O'Halloran, K. D. Tags: Articles Source Type: research

Distinct Expression and Localization of Diacylglycerol Kinase Isozymes in Rat Retina
Recent studies have revealed that phosphoinositide (PI) signaling molecules are expressed in mammalian retinas, suggesting their importance in its signal transduction. We previously showed that diacylglycerol kinase (DGK) isozymes are expressed in distinct patterns in rat retina at the mRNA level. However, little is known about the nature and morphological aspects of DGKs in the retina. For this study, we performed immunohistochemical analyses to investigate in the retina the expression and localization of DGK isozymes at the protein level. Here, we show that both DGKβ and DGK localize in the outer plexiform layer, wi...
Source: Journal of Histochemistry and Cytochemistry - May 24, 2013 Category: Biochemistry Authors: Hozumi, Y., Matsui, H., Sakane, F., Watanabe, M., Goto, K. Tags: Articles Source Type: research