Journal of Histochemistry and Cytochemistry This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Structural Composition of Myocardial Infarction Scar in Middle-aged Male and Female Rats: Does Sex Matter?
The present study was designed to determine whether the structural composition of the scar in middle-aged post–myocardial infraction (MI) rats is affected by the biological sex of the animals. A large MI was induced in 12-month-old male (M-MI) and female (F-MI) Sprague-Dawley rats by ligation of the left coronary artery. Four weeks after the MI, rats with transmural infarctions, greater than 50% of the left ventricular (LV) free wall, were evaluated. The extent of LV remodeling and fractional volumes of fibrillar collagen (FC), myofibroblasts, vascular smooth muscle (SM) cells, and surviving cardiac myocytes (CM) in ...
Source: Journal of Histochemistry and Cytochemistry - October 25, 2013 Category: Biochemistry Authors: Bogatyryov, Y., Tomanek, R. J., Dedkov, E. I. Tags: Articles Source Type: research

Cryptochrome in Sponges: A Key Molecule Linking Photoreception with Phototransduction
Sponges (phylum: Porifera) react to external light or mechanical signals with contractile or metabolic reactions and are devoid of any nervous or muscular system. Furthermore, elements of a photoreception/phototransduction system exist in those animals. Recently, a cryptochrome-based photoreceptor system has been discovered in the demosponge. The assumption that in sponges the siliceous skeleton acts as a substitution for the lack of a nervous system and allows light signals to be transmitted through its glass fiber network is supported by the findings that the first spicules are efficient light waveguides and the second s...
Source: Journal of Histochemistry and Cytochemistry - October 25, 2013 Category: Biochemistry Authors: Muller, W. E. G., Schroder, H. C., Markl, J. S., Grebenjuk, V. A., Korzhev, M., Steffen, R., Wang, X. Tags: Articles Source Type: research

The Urokinase Receptor Homolog Haldisin Is a Novel Differentiation Marker of Stratum Granulosum in Squamous Epithelia
Several members of the Ly-6/uPAR (LU)-protein domain family are differentially expressed in human squamous epithelia. In some cases, they even play important roles in maintaining skin homeostasis, as exemplified by the secreted single domain member, SLURP-1, the deficiency of which is associated with the development of palmoplantar hyperkeratosis in the congenital skin disorder Mal de Meleda. In the present study, we have characterized a new member of the LU-protein domain family, which we find to be predominantly expressed in the stratum granulosum of human skin, thus resembling the expression of SLURP-1. In accordance wi...
Source: Journal of Histochemistry and Cytochemistry - October 25, 2013 Category: Biochemistry Authors: Gardsvoll, H., Kriegbaum, M. C., Hertz, E. P., Alpizar-Alpizar, W., Ploug, M. Tags: Articles Source Type: research

Variations in Both TG1 and TG2 Isozyme-specific In Situ Activities and Protein Expressions during Mouse Embryonic Development
Transglutaminase (TG) is a family of enzymes that catalyzes cross-linking reactions among proteins. Using fluorescent-labeled highly reactive substrate peptides, we recently developed a system to visualize isozyme-specific in situ enzymatic activity. In the present study, we investigated the in situ activities of TG1 (skin-type) and TG2 (tissue-type) using whole mouse sections of various embryonic developmental stages and neonates. In each case, we also successfully used immunostaining of identical whole mouse sections for protein expression after detection of enzymatic activities. In general, the enzymatic activity was co...
Source: Journal of Histochemistry and Cytochemistry - October 25, 2013 Category: Biochemistry Authors: Itoh, M., Tatsukawa, H., Eun-Seo, L., Yamanishi, K., Kojima, S., Hitomi, K. Tags: Articles Source Type: research

Comparison of Histological Techniques to Visualize Iron in Paraffin-embedded Brain Tissue of Patients with Alzheimer's Disease
This article compares three histochemical techniques and ferritin immunohistochemistry to visualize iron in paraffin-embedded human AD brain tissue. The specificity of the histochemical techniques was tested by staining sections after iron extraction. Iron was demonstrated in the white matter, in layers IV/V of the frontal neocortex, in iron containing plaques, and in microglia. In our hands, these structures were best visualized using the Meguro iron stain, a method that has not been described for iron staining in human brain or AD in particular. Ferritin immunohistochemistry stained microglia and iron containing plaques ...
Source: Journal of Histochemistry and Cytochemistry - October 25, 2013 Category: Biochemistry Authors: van Duijn, S., Nabuurs, R. J. A., van Duinen, S. G., Natte, R. Tags: Articles Source Type: research

Antibodies Biotinylated Using a Synthetic Z-domain from Protein A Provide Stringent In Situ Protein Detection
Antibody-based protein profiling on a global scale using immunohistochemistry constitutes an emerging strategy for mapping of the human proteome, which is crucial for an increased understanding of biological processes in the cell. Immunohistochemistry is often performed indirectly using secondary antibodies for detection, with the benefit of signal amplification. Direct immunohistochemistry instead brings the advantage of multiplexing; however, it requires labeling of the primary antibody. Many antibody-labeling kits do not specifically target IgG and may therefore cause labeling of stabilizing proteins present in the anti...
Source: Journal of Histochemistry and Cytochemistry - October 25, 2013 Category: Biochemistry Authors: Andersson, S., Konrad, A., Ashok, N., Ponten, F., Hober, S., Asplund, A. Tags: Articles Source Type: research

Authors' Response to Letter to the Editor on "Image Cytometry Protocols"
(Source: Journal of Histochemistry and Cytochemistry)
Source: Journal of Histochemistry and Cytochemistry - October 1, 2013 Category: Biochemistry Authors: Garrido, R., Zabka, T. S., Tao, J., Fielden, M. R., Fretland, A. J., Albassam, M. Tags: Letter to the Editor Source Type: research

Image Cytometry Protocols
(Source: Journal of Histochemistry and Cytochemistry)
Source: Journal of Histochemistry and Cytochemistry - October 1, 2013 Category: Biochemistry Authors: Van Noorden, C. J. F., Chieco, P. Tags: Letter to the Editor Source Type: research

Dentin Sialophosphoprotein (DSPP) Plays an Essential Role in the Postnatal Development and Maintenance of Mouse Mandibular Condylar Cartilage
Recently, dentin sialophosphoprotein (DSPP) was found to be expressed in the mandibular condylar cartilage (MCC), but the possible roles of this molecule in the formation, growth, and maintenance of the cartilage are largely unclear. To analyze such roles, we examined Dspp null mice compared with wild-type mice to assess the consequences of Dspp deletion on the morphology and structure of the MCC. Our data showed that DSPP is expressed in the prechondroblastic, chondroblastic, hypertrophic layers of the MCC. Dspp null mice exhibited decreases in the amount of MCC, with reduced formation of articular and prechondroblastic l...
Source: Journal of Histochemistry and Cytochemistry - October 1, 2013 Category: Biochemistry Authors: Liu, Q., Gibson, M. P., Sun, H., Qin, C. Tags: Articles Source Type: research

Growth Arrest Specific 1 (GAS1) Is Abundantly Expressed in the Adult Mouse Central Nervous System
Growth arrest specific 1 (GAS1) is a pleiotropic protein that induces apoptosis and cell arrest in different tumors, but it is also involved in the development of the nervous system and other tissues and organs. This dual ability is likely caused by its capacity to interact both by inhibiting the intracellular signaling cascade induced by glial cell-line derived neurotrophic factor and by facilitating the activity of the sonic hedgehog pathway. The presence of GAS1 mRNA has been described in adult mouse brain, and here we corroborated this observation. We then proceeded to determine the distribution of the protein in the a...
Source: Journal of Histochemistry and Cytochemistry - October 1, 2013 Category: Biochemistry Authors: Zarco, N., Bautista, E., Cuellar, M., Vergara, P., Flores-Rodriguez, P., Aguilar-Roblero, R., Segovia, J. Tags: Articles Source Type: research

Laminin Production and Basement Membrane Deposition by Mesenchymal Stem Cells upon Adipogenic Differentiation
The aim was to study laminin (LM) synthesis, integration, and deposition into the basement membrane (BM) during adipogenesis. Human bone marrow-derived mesenchymal stromal cells (MSCs) were induced along the adipogenic lineage. LM chain mRNA and protein levels were followed using quantitative real-time polymerase chain reaction (qRT-PCR), immunofluorescence (IF) staining, transmission electron microscopy (TEM), and immunoprecipitation. MSCs produced low levels of LM mRNAs but were not surrounded by BM in IF and TEM imaging. LM-α4, LM-β1, and LM-1 mRNAs increased during adipogenesis 3.9-, 5.8-, and 2.8-fold by da...
Source: Journal of Histochemistry and Cytochemistry - October 1, 2013 Category: Biochemistry Authors: Noro, A., Sillat, T., Virtanen, I., Ingerpuu, S., Back, N., Konttinen, Y. T., Korhonen, M. Tags: Articles Source Type: research

Laminin and Type IV Collagen Isoform Substitutions Occur in Temporally and Spatially Distinct Patterns in Developing Kidney Glomerular Basement Membranes
Kidney glomerular basement membranes (GBMs) undergo laminin and type IV collagen isoform substitutions during glomerular development, which are believed to be required for maturation of the filtration barrier. Specifically, GBMs of earliest glomeruli contain laminin α1β11 and collagen α1α2α1(IV), whereas mature glomeruli contain laminin α5β21 and collagen α3α4α5(IV). Here, we used confocal microscopy to simultaneously evaluate expression of different laminin and collagen IV isoforms in newborn mouse GBMs. Our results show loss of laminin α1 from GBMs in early ...
Source: Journal of Histochemistry and Cytochemistry - October 1, 2013 Category: Biochemistry Authors: Abrahamson, D. R., St. John, P. L., Stroganova, L., Zelenchuk, A., Steenhard, B. M. Tags: Articles Source Type: research

Quantitative Analysis of Centromeric FISH Spots during the Cell Cycle by Image Cytometry
Two-color fluorescence in situ hybridization (FISH) with chromosome enumeration DNA probes specific to chromosomes 7, 11, 17, and 18 was applied to CAL-51 breast cancer cells to examine whether the fluorescence intensity of FISH spots was associated with cell cycle progression. The fluorescence intensity of each FISH spot was quantitatively analyzed based on the cell cycle stage determined by image cytometry at the single-cell level. The spot intensity of cells in the G2 phase was larger than that in the G0/1 phase. This increased intensity was not seen during the early and mid S phases, whereas the cells in the late S pha...
Source: Journal of Histochemistry and Cytochemistry - October 1, 2013 Category: Biochemistry Authors: Amakawa, G., Ikemoto, K., Ito, H., Furuya, T., Sasaki, K. Tags: Articles Source Type: research

Characterization of the Pre-meiotic S Phase through Incorporation of BrdU during Spermatogenesis in the Rat
Seminiferous tubules in mammals have histological arrangements defined by the associations between somatic cells and germ cells. The processes of DNA synthesis in meiotic and mitotic cells have different features that are not easily distinguishable through morphological means. In order to characterize the pre-meiotic S phase, 5-bromo-2’-deoxyuridine (BrdU) was injected intraperitoneally into Wistar rats, which were sacrificed 30 min, 2 hr, and 24 hr after injection. We found three different labeling patterns. One of these patterns was characterized by a distribution of the label in the form of speckles, most of which...
Source: Journal of Histochemistry and Cytochemistry - August 26, 2013 Category: Biochemistry Authors: Munoz-Velasco, I., Ortiz, R., Echeverria, O. M., Escobar, M. L., Vazquez-Nin, G. H. Tags: Articles Source Type: research

Expression of Fibroblast Growth Factor 9 in Normal Human Lung and Idiopathic Pulmonary Fibrosis
The fibroblast growth factor (FGF) family of signaling ligands contributes significantly to lung development and maintenance in the adult. FGF9 is involved in control of epithelial branching and mesenchymal proliferation and expansion in developing lungs. However, its activity and expression in the normal adult lung and by epithelial and interstitial cells in fibroproliferative diseases like idiopathic pulmonary fibrosis (IPF) are unknown. Tissue samples from normal organ donor human lungs and those of a cohort of patients with mild to severe IPF were sectioned and stained for the immunolocalization of FGF9. In normal lung...
Source: Journal of Histochemistry and Cytochemistry - August 26, 2013 Category: Biochemistry Authors: Coffey, E., Newman, D. R., Sannes, P. L. Tags: Articles Source Type: research