Journal of Histochemistry and Cytochemistry This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Identification of CEACAM5 as a Biomarker for Prewarning and Prognosis in Gastric Cancer
MGd1, a monoclonal antibody raised against gastric cancer cells, possesses a high degree of specificity for gastric cancer (GC). Here we identified that the antigen of MGd1 is CEACAM5, and used MGd1 to investigate the expression of CEACAM5 in non-GC and GC tissues (N=643), as a biomarker for prewarning and prognosis. The expression of CEACAM5 was detected by immunohistochemistry in numerous tissues; its clinicopathological correlation was statistically analyzed. CEACAM5 expression was increased progressively from normal gastric mucosa to chronic atrophic gastritis, intestinal metaplasia, dysplasia and finally to GC (p<0...
Source: Journal of Histochemistry and Cytochemistry - November 25, 2015 Category: Biochemistry Authors: Zhou, J., Fan, X., Chen, N., Zhou, F., Dong, J., Nie, Y., Fan, D. Tags: Articles Source Type: research

Expression of Carcinoembryonic Cell Adhesion Molecule 6 and Alveolar Epithelial Cell Markers in Lungs of Human Infants with Chronic Lung Disease
The membrane protein carcinoembryonic antigen cell adhesion molecule (CEACAM6) is expressed in the epithelium of various tissues, participating in innate immune defense, cell proliferation and differentiation, with overexpression in gastrointestinal tract, pancreatic and lung tumors. It is developmentally and hormonally regulated in fetal human lung, with an apparent increased production in preterm infants with respiratory failure. To further examine the expression and cell localization of CEACAM6, we performed immunohistochemical and biochemical studies in lung specimens from infants with and without chronic lung disease....
Source: Journal of Histochemistry and Cytochemistry - November 25, 2015 Category: Biochemistry Authors: Gonzales, L. W., Gonzalez, R., Barrette, A. M., Wang, P., Dobbs, L., Ballard, P. L. Tags: Articles Source Type: research

Neuro at the Nanoscale: Diffraction-Unlimited Imaging with STED Nanoscopy
Recent breakthroughs in fluorescence microscopy have pushed spatial resolution well beyond the classical limit imposed by diffraction. As a result, the field of nanoscopy has emerged, and diffraction-unlimited resolution is becoming increasingly common in biomedical imaging applications. In this review, we recap the principles behind STED nanoscopy that allow imaging beyond the diffraction limit, and highlight both historical and recent advances made in the field of neuroscience as a result of this technology. (Source: Journal of Histochemistry and Cytochemistry)
Source: Journal of Histochemistry and Cytochemistry - November 25, 2015 Category: Biochemistry Authors: Castro, J. B., Gould, T. J. Tags: Review Source Type: research

Wnt3a Mediates the Inhibitory Effect of Hyperoxia on the Transdifferentiation of AECIIs to AECIs
In conclusion, our results demonstrate that the activated Wnt3a/β-catenin signal may be involved in the hyperoxia-induced inhibition of AECIIs’ transdifferentiation to AECIs. (Source: Journal of Histochemistry and Cytochemistry)
Source: Journal of Histochemistry and Cytochemistry - October 25, 2015 Category: Biochemistry Authors: Xu, W., Zhao, Y., Zhang, B., Xu, B., Yang, Y., Wang, Y., Liu, C. Tags: Articles Source Type: research

Mature Surfactant Protein-B Expression by Immunohistochemistry as a Marker for Surfactant System Development in the Fetal Sheep Lung
Evaluation of the number of type II alveolar epithelial cells (AECs) is an important measure of the lung’s ability to produce surfactant. Immunohistochemical staining of these cells in lung tissue commonly uses antibodies directed against mature surfactant protein (SP)-C, which is regarded as a reliable SP marker of type II AECs in rodents. There has been no study demonstrating reliable markers for surfactant system maturation by immunohistochemistry in the fetal sheep lung despite being widely used as a model to study lung development. Here we examine staining of a panel of surfactant pro-proteins (pro–SP-B an...
Source: Journal of Histochemistry and Cytochemistry - October 25, 2015 Category: Biochemistry Authors: Lock, M. C., McGillick, E. V., Orgeig, S., Zhang, S., McMillen, I. C., Morrison, J. L. Tags: Articles Source Type: research

Human SERPINB12 Is an Abundant Intracellular Serpin Expressed in Most Surface and Glandular Epithelia
The intracellular serine protease inhibitors (serpins) are an important family of proteins that protect cells form proteinase-mediated injury. Understanding the tissue and cellular expression pattern of this protein family can provide important insights into their physiologic roles. For example, high expression in epithelial tissues, such as lung, may suggest a biologic function in cellular defense, secretion, or selective absorption. Although the expression pattern of many of the intracellular serpins has been well described, one member of this class, SERPINB12, has not been carefully examined. We generated a mouse monocl...
Source: Journal of Histochemistry and Cytochemistry - October 25, 2015 Category: Biochemistry Authors: Niehaus, J. Z., Good, M., Jackson, L. E., Ozolek, J. A., Silverman, G. A., Luke, C. J. Tags: Articles Source Type: research

Adipose-Derived Mesenchymal Stem Cells Transplantation Alleviates Renal Injury in Streptozotocin-Induced Diabetic Nephropathy
This study may provide evidence for the treatment of DN using ADMSCs. (Source: Journal of Histochemistry and Cytochemistry)
Source: Journal of Histochemistry and Cytochemistry - October 25, 2015 Category: Biochemistry Authors: Ni, W., Fang, Y., Xie, L., Liu, X., Shan, W., Zeng, R., Liu, J., Liu, X. Tags: Articles Source Type: research

Cellular Factor XIIIA Transglutaminase Localizes in Caveolae and Regulates Caveolin-1 Phosphorylation, Homo-oligomerization and c-Src Signaling in Osteoblasts
In this study, we have investigated the localization and potential role of intracellular FXIII-A. Conventional immunofluorescence microscopy and TIRF microscopy analyses showed that FXIII-A co-localizes with caveolin-1 in specialized membrane structures, caveolae, in differentiating osteoblasts. The caveolae-disrupting agent methyl-β-cyclodextrin abolished FXIII-A staining and co-localization with caveolin-1 from the osteoblast plasma membrane. The presence of FXIII-A in caveolae was confirmed by preparing caveolae-enriched cellular fractions using sucrose density gradient ultracentrifugation followed by western blott...
Source: Journal of Histochemistry and Cytochemistry - October 25, 2015 Category: Biochemistry Authors: Wang, S., Kaartinen, M. T. Tags: Articles Source Type: research

Erratum
In the Editorial, "Histochemical Insights into Pancreatic Islet Biology," by Rebecca L. Hull and Denis G. Baskin, J Histochem Cytochem, August 2015, 63:541-542, there were several errors in the references section. (DOI: 10.1369/0022155415586442) First, the following references did not include page numbers: Asadi A, Bruin JE, Kieffer TJ (2015). Characterization of antibodies to products of proinsulin processing using immunofluorescent staining of pancreas in multiple species. J Histochem Cytochem 63:646-662. Baskin DG (2015). A historical perspective on identification of cell types in pancreatic islets of Langerhans by stai...
Source: Journal of Histochemistry and Cytochemistry - September 25, 2015 Category: Biochemistry Tags: Erratum Source Type: research

Epitope Recognition in the Human-Pig Comparison Model on Fixed and Embedded Material
The conditions and the specificity by which an antibody binds to its target protein in routinely fixed and embedded tissues are unknown. Direct methods, such as staining in a knock-out animal or in vitro peptide scanning of the epitope, are costly and impractical. We aimed to elucidate antibody specificity and binding conditions using tissue staining and public genomic and immunological databases by comparing human and pig—the farmed mammal evolutionarily closest to humans besides apes. We used a database of 146 anti-human antibodies and found that antibodies tolerate partially conserved amino acid substitutions but ...
Source: Journal of Histochemistry and Cytochemistry - September 25, 2015 Category: Biochemistry Authors: Scalia, C. R., Gendusa, R., Basciu, M., Riva, L., Tusa, L., Musaro, A., Veronese, S., Formenti, A., D'Angelo, D., Ronzio, A. G., Cattoretti, G., Bolognesi, M. M. Tags: Articles Source Type: research

Alternative Splicing, Expression and Cellular Localization of Calneuron-1 in the Rat and Human Brain
Calneuron-1 and -2 are members of the neuronal calcium-binding protein family (nCaBP). They are transmembrane Calmodulin-like EF-hand Ca2+-sensors, and a function in the control of Golgi-to-plasma membrane vesicle trafficking has been assigned to both proteins. In this paper, we describe the distribution of Calneuron-1 in rat and human brains. We show that Calneuron-1 is ubiquitously expressed in all brain regions examined. The protein is most abundant in Purkinje cells of the cerebellum and principal neurons of the cortex and limbic brain whereas no expression in glial cells is apparent. In addition, we identify two novel...
Source: Journal of Histochemistry and Cytochemistry - September 25, 2015 Category: Biochemistry Authors: Hradsky, J., Bernstein, H.-G., Marunde, M., Mikhaylova, M., Kreutz, M. R. Tags: Articles Source Type: research

Native Immunogold Labeling of Cell Surface Proteins and Viral Glycoproteins for Cryo-Electron Microscopy and Cryo-Electron Tomography Applications
We describe a method for combining immunogold labeling with cryo-electron microscopy (cryo-EM) and cryo-electron tomography (cryo-ET) of the surface proteins of intact mammalian cells or the surface glycoproteins of assembling and budding viruses in the context of virus-infected mammalian cells cultured on EM grids. In this method, the cells were maintained in culture media at physiologically relevant temperatures while sequentially incubated with the primary and secondary antibodies. Subsequently, the immunogold-labeled specimens were vitrified and observed under cryo-conditions in the transmission electron microscope. Cr...
Source: Journal of Histochemistry and Cytochemistry - September 25, 2015 Category: Biochemistry Authors: Yi, H., Strauss, J. D., Ke, Z., Alonas, E., Dillard, R. S., Hampton, C. M., Lamb, K. M., Hammonds, J. E., Santangelo, P. J., Spearman, P. W., Wright, E. R. Tags: Articles Source Type: research

MALDI-Mass Spectrometry Imaging Identifies Vitronectin as a Common Constituent of Amyloid Deposits
Amyloids are pathological intra- and extracellular fibrillar aggregates of polypeptides with a cross-β-sheet structure and characteristic tinctorial properties. The amyloid deposits commonly enclose several non-fibrillar components of the extracellular matrix. Their potential to regulate the formation and aggregation process of amyloid fibrils is still poorly understood. For a better understanding of the role of the extracellular matrix in amyloidosis, it is essential to gain deeper insights into the composition of amyloid deposits. Here, we utilized matrix-assisted laser desorption and ionization mass spectrometry im...
Source: Journal of Histochemistry and Cytochemistry - September 25, 2015 Category: Biochemistry Authors: Winter, M., Tholey, A., Kruger, S., Schmidt, H., Rocken, C. Tags: Articles Source Type: research

Review of Matrix-Assisted Laser Desorption Ionization-Imaging Mass Spectrometry for Lipid Biochemical Histopathology
Matrix-Assisted Laser Desorption Ionization-Imaging Mass Spectrometry (MALDI-IMS) is a rapidly evolving method used for the in situ visualization and localization of molecules such as drugs, lipids, peptides, and proteins in tissue sections. Therefore, molecules such as lipids, for which antibodies and other convenient detection reagents do not exist, can be detected, quantified, and correlated with histopathology and disease mechanisms. Furthermore, MALDI-IMS has the potential to enhance our understanding of disease pathogenesis through the use of "biochemical histopathology". Herein, we review the underlying concepts, ba...
Source: Journal of Histochemistry and Cytochemistry - September 25, 2015 Category: Biochemistry Authors: Yalcin, E. B., de la Monte, S. M. Tags: Reviews Source Type: research

The Study of Glioma by Xenotransplantation in Zebrafish Early Life Stages
Zebrafish (Danio rerio) and their transparent embryos are becoming an increasingly popular tool for studying processes involved in tumor progression and in the search for novel tumor treatment approaches. The xenotransplantation of fluorescently labeled mammalian cancer cells into zebrafish embryos is an approach enabling relatively high-throughput in vivo analyses. The small size of the embryos as well as the relative simplicity of their manipulation and maintenance allow for large numbers of embryos to be processed efficiently in a short time and at low cost. Furthermore, the possibility of fluorescence microscopic imagi...
Source: Journal of Histochemistry and Cytochemistry - September 25, 2015 Category: Biochemistry Authors: Vittori, M., Motaln, H., Turnsek, T. L. Tags: Reviews Source Type: research