Journal of Histochemistry and Cytochemistry This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Monitoring mRNA Translation in Neuronal Processes Using Fluorescent Non-Canonical Amino Acid Tagging
This report presents the utility of employing microfluidic chambers for the isolation and treatment of single neuronal cellular compartments. Furthermore, it is demonstrated that a protein synthesis assay, based on fluorescent non-canonical amino acid tagging (FUNCAT), can be combined with this cell culture system to label nascent proteins within a discrete structural and functional domain of the neuron. Together, these techniques could be employed for the detection of protein synthesis within developing and mature neurites, offering an effective approach to elucidate novel mechanisms controlling synaptic maintenance and p...
Source: Journal of Histochemistry and Cytochemistry - April 25, 2016 Category: Biochemistry Authors: Kos, A., Wanke, K. A., Gioio, A., Martens, G. J., Kaplan, B. B., Aschrafi, A. Tags: Articles Source Type: research

Quantitative Time-Resolved Fluorescence Imaging of Androgen Receptor and Prostate-Specific Antigen in Prostate Tissue Sections
This study demonstrates the feasibility of developing reproducible protocols for TRF imaging and automated image analysis to study the expression of AR and PSA in benign and malignant prostate. It also highlighted the differences in AR and PSA protein expression within AMACR- and AMACR+ cancer regions. (Source: Journal of Histochemistry and Cytochemistry)
Source: Journal of Histochemistry and Cytochemistry - April 25, 2016 Category: Biochemistry Authors: Krzyzanowska, A., Lippolis, G., Helczynski, L., Anand, A., Peltola, M., Pettersson, K., Lilja, H., Bjartell, A. Tags: Articles Source Type: research

Detection of Phenotypic Alterations Using High-Content Analysis of Whole-Slide Images
Tumors exhibit spatial heterogeneity, as manifested in immunohistochemistry (IHC) staining patterns. Current IHC quantification methods lose information by reducing this heterogeneity in each whole-slide image (WSI) or in selective fields of view to a single staining index. The aim of this study was to investigate the sensitivity of an IHC quantification method that uses this heterogeneity to reliably compare IHC staining patterns. We virtually partitioned WSIs by a grid of square tiles, and computed the staining index distributions to quantify heterogeneities. We used samples from these distributions as inputs to non-para...
Source: Journal of Histochemistry and Cytochemistry - April 25, 2016 Category: Biochemistry Authors: Shirinifard, A., Thiagarajan, S., Vogel, P., Sablauer, A. Tags: Articles Source Type: research

A Systematic Characterization of Aquaporin-9 Expression in Human Normal and Pathological Tissues
AQP9 is known to facilitate hepatocyte glycerol uptake. Murine AQP9 protein expression has been verified in liver, skin, epididymis, epidermis and neuronal cells using knockout mice. Further expression sites have been reported in humans. We aimed to verify AQP9 expression in a large set of human normal organs, different cancer types, rheumatoid arthritis synovial biopsies as well as in cell lines and primary cells. Combining standardized immunohistochemistry with high-throughput mRNA sequencing, we found that AQP9 expression in normal tissues was limited, with high membranous expression only in hepatocytes. In cancer tissu...
Source: Journal of Histochemistry and Cytochemistry - April 25, 2016 Category: Biochemistry Authors: Lindskog, C., Asplund, A., Catrina, A., Nielsen, S., Rützler, M. Tags: Articles Source Type: research

Argonaute2 Protein in Rat Spermatogenic Cells Is Localized to Nuage Structures and LAMP2-Positive Vesicles Surrounding Chromatoid Bodies
Localization of Argonaute2 (AGO2) protein—an essential component for the processing of small interfering RNA (siRNA)-directed RNA interference (RNAi) in RNA-induced silencing complex (RISC) in nuage of rat spermatogenic cells—was evaluated by immunofluorescence microscopy (IFM) and immunoelectron microscopy (IEM). AGO2 was shown, for the first time, to be localized to four previously classified types of nuage: irregularly shaped perinuclear granules (ISPGs), intermitochondrial cement (IMC), satellite bodies (SBs), and chromatoid bodies (CBs). Dual IEM staining for AGO2/Maelstrom (MAEL) protein or AGO2/MIWI prot...
Source: Journal of Histochemistry and Cytochemistry - March 29, 2016 Category: Biochemistry Authors: Fujii, Y., Onohara, Y., Fujita, H., Yokota, S. Tags: Articles Source Type: research

Comparison of Predictive Immunohistochemical Marker Expression of Primary Breast Cancer and Paired Distant Metastasis using Surgical Material: A Practice-Based Study
Parallel studies of primary breast carcinomas and corresponding distant metastases samples reveal considerable differences. Our aim was to highlight this issue from another perspective and provide further data based on 98 patient samples: 69 primary breast carcinoma and 85 distant metastases from bone, central nervous system (CNS) and lung (56 paired). Two independent series of immunohistochemical reactions with different antibodies for estrogen receptor (ER), progesterone receptor (PgR) and human epidermal growth factor receptor 2 (Her2), along with HER2 fluroscence in situ hybridization (FISH) were performed on tissue mi...
Source: Journal of Histochemistry and Cytochemistry - March 29, 2016 Category: Biochemistry Authors: Kulka, J., Szekely, B., Lukacs, L. V., Kiss, O., Tokes, A.-M., Vincze, E., Turanyi, E., Fillinger, J., Hanzely, Z., Arato, G., Szendroi, M., Gyorffy, B., Szasz, A. M. Tags: Articles Source Type: research

Bardoxolone Methyl Prevents High-Fat Diet-Induced Colon Inflammation in Mice
Obesity induces chronic, low-grade inflammation, which increases the risk of colon cancer. We investigated the preventive effects of Bardoxolone methyl (BARD) on high-fat diet (HFD)-induced inflammation in a mouse colon. Male C57BL/6J mice (n=7) were fed a HFD (HFD group), HFD plus BARD (10 mg/kg) in drinking water (HFD/BARD group), or normal laboratory chow diet (LFD group) for 21 weeks. In HFD mice, BARD reduced colon thickness and decreased colon weight per length. This was associated with an increase in colon crypt depth and the number of goblet cells per crypt. BARD reduced the expression of F4/80 and CD11c but increa...
Source: Journal of Histochemistry and Cytochemistry - March 29, 2016 Category: Biochemistry Authors: Dinh, C. H. L., Yu, Y., Szabo, A., Zhang, Q., Zhang, P., Huang, X.-F. Tags: Articles Source Type: research

MEPE Localization in the Craniofacial Complex and Function in Tooth Dentin Formation
Matrix extracellular phosphoglycoprotein (MEPE) is an extracellular matrix protein found in dental and skeletal tissues. Although information regarding the role of MEPE in bone and disorders of phosphate metabolism is emerging, the role of MEPE in dental tissues remains unclear. We performed RNA in situ hybridization and immunohistochemistry analyses to delineate the expression pattern of MEPE during embryonic and postnatal development in craniofacial mineralizing tissues. Mepe RNA expression was seen within teeth from cap through root formation in association with odontoblasts and cellular cementoblasts. More intense expr...
Source: Journal of Histochemistry and Cytochemistry - March 29, 2016 Category: Biochemistry Authors: Gullard, A., Gluhak-Heinrich, J., Papagerakis, S., Sohn, P., Unterbrink, A., Chen, S., MacDougall, M. Tags: Articles Source Type: research

Reproducibility: It Is Just Good Science
(Source: Journal of Histochemistry and Cytochemistry)
Source: Journal of Histochemistry and Cytochemistry - March 29, 2016 Category: Biochemistry Authors: Hewitt, S. M. Tags: Editorial Source Type: research

Ulex Europaeus Agglutinin-1 Is a Reliable Taste Bud Marker for In Situ Hybridization Analyses
Taste signals are received by taste buds. To better understand the taste reception system, expression patterns of taste-related molecules are determined by in situ hybridization (ISH) analyses at the histological level. Nevertheless, even though ISH is essential for determining mRNA expression, few taste bud markers can be applied together with ISH. Ulex europaeus agglutinin-1 (UEA-1) appears to be a reliable murine taste bud marker based on immunohistochemistry (IHC) analyses. However, there is no evidence as to whether UEA-1 can be used for ISH. Thus, the present study evaluated UEA-1 using various histochemical methods,...
Source: Journal of Histochemistry and Cytochemistry - February 24, 2016 Category: Biochemistry Authors: Yoshimoto, J., Okada, S., Kishi, M., Misaka, T. Tags: Articles Source Type: research

Stem Cell-Associated Marker Expression in Canine Hair Follicles
Functional hair follicle (HF) stem cells (SCs) are crucial to maintain the constant recurring growth of hair. In mice and humans, SC subpopulations with different biomarker expression profiles have been identified in discrete anatomic compartments of the HF. The rare studies investigating canine HF SCs have shown similarities in biomarker expression profiles to that of mouse and human SCs. The aim of our study was to broaden the current repertoire of SC-associated markers and their expression patterns in the dog. We combined analyses on the expression levels of CD34, K15, Sox9, CD200, Nestin, LGR5 and LGR6 in canine skin u...
Source: Journal of Histochemistry and Cytochemistry - February 24, 2016 Category: Biochemistry Authors: Gerhards, N. M., Sayar, B. S., Origgi, F. C., Galichet, A., Müller, E. J., Welle, M. M., Wiener, D. J. Tags: Articles Source Type: research

Sclerostin Immunoreactivity Increases in Cortical Bone Osteocytes and Decreases in Articular Cartilage Chondrocytes in Aging Mice
Sclerostin is a 24-kDa secreted glycoprotein that has been identified as a negative modulator of new bone formation and may play a major role in age-related decline in skeletal function. Although serum levels of sclerostin markedly increase with age, relatively little is known about whether cells in the skeleton change their expression of sclerostin with aging. Using immunohistochemistry and confocal microscopy, we explored sclerostin immunoreactivity (sclerostin-IR) in the femurs of 4-, 9-, and 24-month-old adult C3H/HeJ male mice. In the femur, the only two cell types that expressed detectable levels of sclerostin-IR wer...
Source: Journal of Histochemistry and Cytochemistry - February 24, 2016 Category: Biochemistry Authors: Thompson, M. L., Jimenez-Andrade, J. M., Mantyh, P. W. Tags: Articles Source Type: research

An Improved Immunostaining and Imaging Methodology to Determine Cell and Protein Distributions within the Bone Environment
Bone is a dynamic tissue that undergoes multiple changes throughout its lifetime. Its maintenance requires a tight regulation between the cells embedded within the bone matrix, and an imbalance among these cells may lead to bone diseases such as osteoporosis. Identifying cell populations and their proteins within bone is necessary for understanding bone biology. Immunolabeling is one approach used to visualize proteins in tissues. Efficient immunolabeling of bone samples often requires decalcification, which may lead to changes in the structural morphology of the bone. Recently, methyl-methacrylate embedding of non-decalci...
Source: Journal of Histochemistry and Cytochemistry - February 24, 2016 Category: Biochemistry Authors: Akkiraju, H., Bonor, J., Nohe, A. Tags: Articles Source Type: research

TGF-{beta}/SMAD Pathway and Its Regulation in Hepatic Fibrosis
Transforming growth factor-beta1 (TGF-β1), a key member in the TGF-β superfamily, plays a critical role in the development of hepatic fibrosis. Its expression is consistently elevated in affected organs, which correlates with increased extracellular matrix deposition. SMAD proteins have been studied extensively as pivotal intracellular effectors of TGF-β1, acting as transcription factors. In the context of hepatic fibrosis, SMAD3 and SMAD4 are pro-fibrotic, whereas SMAD2 and SMAD7 are protective. Deletion of SMAD3 inhibits type I collagen expression and blocks epithelial-myofibroblast transition. In contrast...
Source: Journal of Histochemistry and Cytochemistry - February 24, 2016 Category: Biochemistry Authors: Xu, F., Liu, C., Zhou, D., Zhang, L. Tags: Review Source Type: research

miR-21 Is Linked to Glioma Angiogenesis: A Co-Localization Study
MicroRNA-21 (miR-21) is the most consistently over-expressed microRNA (miRNA) in malignant gliomas. We have previously reported that miR-21 is upregulated in glioma vessels and subsets of glioma cells. To better understand the role of miR-21 in glioma angiogenesis and to characterize miR-21-positive tumor cells, we systematically stained consecutive serial sections from ten astrocytomas for miR-21, hypoxia-inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF), phosphatase and tensin homolog (PTEN), octamer-binding transcription factor 4 (Oct4), sex-determining region Y box 2 (Sox2) and CD133. ...
Source: Journal of Histochemistry and Cytochemistry - January 26, 2016 Category: Biochemistry Authors: Hermansen, S. K., Nielsen, B. S., Aaberg-Jessen, C., Kristensen, B. W. Tags: Articles Source Type: research