Journal of Histochemistry and Cytochemistry This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Molecular Cloning and Subcellular Localization of Tektin2-Binding Protein 1 (Ccdc 172) in Rat Spermatozoa
In this study, we characterized TEKT2BP1, which is registered as a coiled-coil domain-containing protein 172 (Ccdc172) in the latest database. RT-PCR analysis indicated that TEKT2BP1 was predominantly expressed in rat testis and that its expression was increased after 3 weeks of postnatal development. Immunocytochemical studies discovered that TEKT2BP1 localized in the middle piece of rat spermatozoa, predominantly concentrated at the mitochondria sheath of the flagella. We hypothesize that the TEKT2-TEKT2BP1 complex might be involved in the structural linkage between the ODF and mitochondria in the middle piece of the spe...
Source: Journal of Histochemistry and Cytochemistry - March 25, 2014 Category: Biochemistry Authors: Yamaguchi, A., Kaneko, T., Inai, T., Iida, H. Tags: Articles Source Type: research

Immunohistological Localization of Endogenous Unlabeled Stem Cells in Wounded Skin
This study demonstrates the feasibility of using immunohistological staining to determine the location of SCs in wounded skin and the intracellular distribution of their molecular markers. (Source: Journal of Histochemistry and Cytochemistry)
Source: Journal of Histochemistry and Cytochemistry - March 25, 2014 Category: Biochemistry Authors: Hong, S., Alapure, B. V., Lu, Y., Tian, H., Wang, Q. Tags: Articles Source Type: research

Mitochondrial Staining Allows Robust Elimination of Apoptotic and Damaged Cells during Cell Sorting
High-speed fluorescence-activated cell sorting is relevant for a plethora of applications, such as PCR-based techniques, microarrays, cloning, and propagation of selected cell populations. We suggest a simple cell-sorting technique to eliminate early and late apoptotic and necrotic cells, with good signal-to-noise ratio and a high-purity yield. The mitochondrial potential dye, TMRE (tetramethylrhodamine ethyl ester perchlorate), was used to separate viable and non-apoptotic cells from the cell sorting samples. TMRE staining is reversible and does not affect cell proliferation and viability. Sorted TMRE+ cells contained a n...
Source: Journal of Histochemistry and Cytochemistry - March 25, 2014 Category: Biochemistry Authors: Barteneva, N. S., Ponomarev, E. D., Tsytsykova, A., Armant, M., Vorobjev, I. A. Tags: Articles Source Type: research

Bioimaging of Fluorescence-Labeled Mitochondria in Subcutaneously Grafted Murine Melanoma Cells by the "In Vivo Cryotechnique"
The microenvironments of organs with blood flow affect the metabolic profiles of cancer cells, which are influenced by mitochondrial functions. However, histopathological analyses of these aspects have been hampered by technical artifacts of conventional fixation and dehydration, including ischemia/anoxia. The purpose of this study was to combine the in vivo cryotechnique (IVCT) with fluorescent protein expression, and examine fluorescently labeled mitochondria in grafted melanoma tumors. The intensity of fluorescent proteins was maintained well in cultured B16-BL6 cells after cryotechniques followed by freeze-substitution...
Source: Journal of Histochemistry and Cytochemistry - March 25, 2014 Category: Biochemistry Authors: Lei, T., Huang, Z., Ohno, N., Wu, B., Sakoh, T., Saitoh, Y., Saiki, I., Ohno, S. Tags: Articles Source Type: research

Consumption of Acidic Water Alters the Gut Microbiome and Decreases the Risk of Diabetes in NOD Mice
Infant formula and breastfeeding are environmental factors that influence the incidence of Type 1 Diabetes (T1D) as well as the acidity of newborn diets. To determine if altering the intestinal microbiome is one mechanism through which an acidic liquid plays a role in T1D, we placed non-obese diabetic (NOD)/ShiLtJt mice on neutral (N) or acidified H2O and monitored the impact on microbial composition and diabetes incidence. NOD-N mice showed an increased development of diabetes, while exhibiting a decrease in Firmicutes and an increase in Bacteroidetes, Actinobacteria, and Proteobacteria from as early as 2 weeks of age. NO...
Source: Journal of Histochemistry and Cytochemistry - March 25, 2014 Category: Biochemistry Authors: Wolf, K. J., Daft, J. G., Tanner, S. M., Hartmann, R., Khafipour, E., Lorenz, R. G. Tags: Articles Source Type: research

Distribution of Corticosteroid Receptors in Mature Oligodendrocytes and Oligodendrocyte Progenitors of the Adult Mouse Brain
The expression of glucocorticoid receptors (GRs) was investigated immunohistochemically in two different lineages of oligodendrocytes, using carbonic anhydrase (CA) II and neuron glial antigen (NG) 2 as markers of mature oligodendrocytes and oligodendrocyte progenitors, respectively. We focused on the gray matter regions, including CA1, CA3 and the dentate gyrus of the hippocampus, the primary somatosensory cortex barrel field and the basolateral amygdala, and the white matter regions, including the corpus callosum, external capsule and fimbria of the hippocampus. More than 80% of CAII-immunoreactive (IR) cells and more th...
Source: Journal of Histochemistry and Cytochemistry - February 25, 2014 Category: Biochemistry Authors: Matsusue, Y., Horii-Hayashi, N., Kirita, T., Nishi, M. Tags: Articles Source Type: research

Carbonic Anhydrase IX Expression is Associated with Favorable Prognostic Factors in Small Intestinal Carcinoma
Tumor hypoxia is associated with more aggressive behavior and resistance to chemotherapy and radiotherapy. Carbonic anhydrase IX (CA9) level increases under hypoxia and is related to poor prognostic factors. The aim of this study was to evaluate the expression of CA9 and to identify its prognostic significance in small intestinal carcinomas (SICs). CA9 expression was observed in 36% (63/175) of SICs. CA9 expression showed significant correlation with well- and moderately differentiated tumors compared with poorly differentiated tumors (p=0.039), tumors with no lymph node metastasis (p=0.005), and lower stage carcinomas (p=...
Source: Journal of Histochemistry and Cytochemistry - February 25, 2014 Category: Biochemistry Authors: Gu, M. J., Kwon, K. W. Tags: Articles Source Type: research

A Flexible Mouse-On-Mouse Immunohistochemical Staining Technique Adaptable to Biotin-Free Reagents, Immunofluorescence, and Multiple Antibody Staining
Immunohistochemistry on mouse tissue utilizing mouse monoclonal antibodies presents a challenge. Secondary antibodies directed against the mouse monoclonal primary antibody of interest will also detect endogenous mouse immunoglobulin in the tissue. This can lead to significant spurious staining. Therefore, a "mouse-on-mouse" staining strategy is needed to yield credible data. This paper presents a method that is easy to use and highly flexible to accommodate both an avidin-biotin detection system as well as a biotin-free polymer detection system. The mouse primary antibody is first combined with an Fab fragment of an anti-...
Source: Journal of Histochemistry and Cytochemistry - February 25, 2014 Category: Biochemistry Authors: Goodpaster, T., Randolph-Habecker, J. Tags: Articles Source Type: research

Development of DNA Damage Response Signaling Biomarkers using Automated, Quantitative Image Analysis
In this study, both AQUA and Tissue Studio algorithms were used to quantify the DDR in radiation-damaged skin fibroblasts, melanoma cell lines, moles, and primary and metastatic melanomas. Digital image analysis results for three markers of DDR (H2AX, P-ATM, P-Chk2) correlated with immunoblot data for irradiated fibroblasts, whereas only H2AX and P-Chk2 correlated with immunoblot data in melanoma cell lines. Melanoma cell lines displayed substantial variation in H2AX and P-Chk2 expression, and P-Chk2 expression was significantly correlated with radioresistance. Moles, primary melanomas, and melanoma metastases in brain, lu...
Source: Journal of Histochemistry and Cytochemistry - February 25, 2014 Category: Biochemistry Authors: Nikolaishvilli-Feinberg, N., Cohen, S. M., Midkiff, B., Zhou, Y., Olorvida, M., Ibrahim, J. G., Omolo, B., Shields, J. M., Thomas, N. E., Groben, P. A., Kaufmann, W. K., Miller, C. R. Tags: Articles Source Type: research

Protein Kinase C, Focal Adhesions and the Regulation of Cell Migration
Cell adhesion to extracellular matrix is a complex process involving protrusive activity driven by the actin cytoskeleton, engagement of specific receptors, followed by signaling and cytoskeletal organization. Thereafter, contractile and endocytic/recycling activities may facilitate migration and adhesion turnover. Focal adhesions, or focal contacts, are widespread organelles at the cell-matrix interface. They arise as a result of receptor interactions with matrix ligands, together with clustering. Recent analysis shows that focal adhesions contain a very large number of protein components in their intracellular compartmen...
Source: Journal of Histochemistry and Cytochemistry - February 25, 2014 Category: Biochemistry Authors: Fogh, B. S., Multhaupt, H. A. B., Couchman, J. R. Tags: Review Source Type: research

An Investigation of the Specificity of Research Antibodies against NF-{kappa}B-subunit p65
(Source: Journal of Histochemistry and Cytochemistry)
Source: Journal of Histochemistry and Cytochemistry - January 25, 2014 Category: Biochemistry Authors: Slotta, C., Muller, J., Tran, L., Hauser, S., Widera, D., Kaltschmidt, B., Kaltschmidt, C. Tags: Letter to the Editor Source Type: research

KSGal6ST Is Essential for the 6-Sulfation of Galactose within Keratan Sulfate in Early Postnatal Brain
Keratan sulfate (KS) comprises repeating disaccharides of galactose (Gal) and N-acetylglucosamine (GlcNAc). Residues of Gal and GlcNAc in KS are potentially modified with sulfate at their C-6 positions. The 5D4 monoclonal antibody recognizes KS structures containing Gal and GlcNAc, both 6-sulfated, and has been used most extensively to evaluate KS expression in mammalian brains. We previously showed that GlcNAc6ST1 is an enzyme responsible for the synthesis of the 5D4 epitope in developing brain and in the adult brain, where it is induced after injury. It has been unclear which sulfotransferase is responsible for Gal-6-sul...
Source: Journal of Histochemistry and Cytochemistry - January 25, 2014 Category: Biochemistry Authors: Hoshino, H., Foyez, T., Ohtake-Niimi, S., Takeda-Uchimura, Y., Michikawa, M., Kadomatsu, K., Uchimura, K. Tags: Articles Source Type: research

Vanilloid Receptor-1 (TRPV1) Expression and Function in the Vasculature of the Rat
This study characterized the arteriolar TRPV1 function and expression in the rat. TRPV1 mRNA was expressed in various vascular beds. Six commercially available antibodies were tested for TRPV1 specificity. Two of them were specific (immunostaining was abolished by blocking peptides) for neuronal TRPV1 and one recognized vascular TRPV1. TRPV1 was expressed in blood vessels in the skeletal muscle, mesenteric and skin tissues, as well as in the aorta and carotid arteries. TRPV1 expression was found to be regulated at the level of individual blood vessels, where some vessels expressed, while others did not express TRPV1 in the...
Source: Journal of Histochemistry and Cytochemistry - January 25, 2014 Category: Biochemistry Authors: Toth, A., Czikora, A., Pasztor, E. T., Dienes, B., Bai, P., Csernoch, L., Rutkai, I., Csato, V., Manyine, I. S., Porszasz, R., Edes, I., Papp, Z., Boczan, J. Tags: Articles Source Type: research

Therapeutic Effects of Suppressors of Cytokine Signaling in Diabetic Nephropathy
Inflammation is an important pathophysiological mechanism in diabetic nephropathy (DN). Tubular epithelial cell-myofibroblast transdifferentiation (TEMT), which can be induced by many cytokines, is an important event in DN. Oncostatin M (OSM), an inflammatory cytokine, can induce TEMT in vitro. The suppressors of cytokine signaling (SOCS) proteins are negative-feedback regulators of cytokine signaling. The purpose of this study was to investigate the role of SOCS in DN. The results demonstrated that overexpression of SOCS ameliorated proteinuria, the expression of α-SMA and OSM in tubular epithelial cells, and inters...
Source: Journal of Histochemistry and Cytochemistry - January 25, 2014 Category: Biochemistry Authors: Liu, Q., Xing, L., Wang, L., Yao, F., Liu, S., Hao, J., Liu, W., Duan, H. Tags: Articles Source Type: research

Connective Tissue Growth Factor Is Involved in Structural Retinal Vascular Changes in Long-Term Experimental Diabetes
Early retinal vascular changes in the development of diabetic retinopathy (DR) include capillary basal lamina (BL) thickening, pericyte loss and the development of acellular capillaries. Expression of the CCN (connective tissue growth factor/cysteine-rich 61/nephroblastoma overexpressed) family member CCN2 or connective tissue growth factor (CTGF), a potent inducer of the expression of BL components, is upregulated early in diabetes. Diabetic mice lacking one functional CTGF allele (CTGF+/–) do not show this BL thickening. As early events in DR may be interrelated, we hypothesized that CTGF plays a role in the pathol...
Source: Journal of Histochemistry and Cytochemistry - January 25, 2014 Category: Biochemistry Authors: Van Geest, R. J., Leeuwis, J. W., Dendooven, A., Pfister, F., Bosch, K., Hoeben, K. A., Vogels, I. M. C., Van der Giezen, D. M., Dietrich, N., Hammes, H.-P., Goldschmeding, R., Klaassen, I., Van Noorden, C. J. F., Schlingemann, R. O. Tags: Articles Source Type: research