Journal of Histochemistry and Cytochemistry This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Tissue- and Cell-Specific Co-localization of Intracellular Gelatinolytic Activity and Matrix Metalloproteinase 2
Matrix metalloproteinase 2 (MMP-2) is a proteolytic enzyme that degrades extracellular matrix proteins. Recent studies indicate that MMP-2 also has a role in intracellular proteolysis during various pathological conditions, such as ischemic injuries in heart and brain and in tumor growth. The present study was performed to map the distribution of intracellular MMP-2 activity in various mouse tissues and cells under physiological conditions. Samples from normal brain, heart, lung, liver, spleen, pancreas, kidney, adrenal gland, thyroid gland, gonads, oral mucosa, salivary glands, esophagus, intestines, and skin were subject...
Source: Journal of Histochemistry and Cytochemistry - May 24, 2013 Category: Biochemistry Authors: Solli, A. I., Fadnes, B., Winberg, J.-O., Uhlin-Hansen, L., Hadler-Olsen, E. Tags: Articles Source Type: research

Losartan Affects Glomerular AKT and mTOR Phosphorylation in an Experimental Model of Type 1 Diabetic Nephropathy
In conclusion, a new body of evidence indicates the differential activation of AKT-mTOR in glomeruli and podocytes of healthy and diabetic animals in response to losartan. (Source: Journal of Histochemistry and Cytochemistry)
Source: Journal of Histochemistry and Cytochemistry - May 24, 2013 Category: Biochemistry Authors: Mavroeidi, V., Petrakis, I., Stylianou, K., Katsarou, T., Giannakakis, K., Perakis, K., Vardaki, E., Stratigis, S., Ganotakis, E., Papavasiliou, S., Daphnis, E. Tags: Articles Source Type: research

Transient Receptor Potential Vanilloid Type 1 Channel (TRPV1) Immunolocalization in the Murine Enteric Nervous System Is Affected by the Targeted C-terminal Epitope of the Applied Antibody
The expression of transient receptor potential vanilloid type 1 channel (TRPV1) in the enteric nervous system is still the subject of debate. Although a number of studies have reported that TRPV1 is limited to extrinsic afferent fibers, other studies argue for an intrinsic expression of TRPV1. In the present study, reverse transcriptase PCR was employed to establish the expression of TRPV1 mRNA throughout the gastrointestinal tract. Using two antibodies directed against different epitopes of TRPV1, we were able to show at the protein level that the observed distribution pattern of TRPV1 is dependent on the antibody used in...
Source: Journal of Histochemistry and Cytochemistry - May 24, 2013 Category: Biochemistry Authors: Buckinx, R., Van Nassauw, L., Avula, L. R., Alpaerts, K., Adriaensen, D., Timmermans, J.-P. Tags: Articles Source Type: research

Technologies for Investigating the Physiological Barriers to Efficient Lipid Nanoparticle-siRNA Delivery
Small interfering RNA (siRNA) therapeutics have advanced from bench to clinical trials in recent years, along with new tools developed to enable detection of siRNA delivered at the organ, cell, and subcellular levels. Preclinical models of siRNA delivery have benefitted from methodologies such as stem-loop quantitative polymerase chain reaction, histological in situ immunofluorescent staining, endosomal escape assay, and RNA-induced silencing complex loading assay. These technologies have accelerated the detection and optimization of siRNA platforms to overcome the challenges associated with delivering therapeutic oligonuc...
Source: Journal of Histochemistry and Cytochemistry - May 24, 2013 Category: Biochemistry Authors: Shi, B., Abrams, M. Tags: Review Source Type: research

Corrigendum
Stage-specific expression of dynein light chain-1 and its interacting kinase, p21-activated kinase-1, in rodent testes: implications in spermiogenesis. Rui-An Wang, Ming Zhao, Marvin L. Meistrich, and Rakesh Kumar. J Histochem Cytochem. 53(10):1235–1243. DOI:10.1369/jhc.5A6688.2005. (Source: Journal of Histochemistry and Cytochemistry)
Source: Journal of Histochemistry and Cytochemistry - April 24, 2013 Category: Biochemistry Tags: Corrigendum Source Type: research

MicroCT versus sTSLIM 3D Imaging of the Mouse Cochlea
We made a qualitative and quantitative comparison between a state-of-the-art implementation of micro-Computed Tomography (microCT) and the scanning Thin-Sheet Laser Imaging Microscopy (sTSLIM) method, applied to mouse cochleae. Both imaging methods are non-destructive and perform optical sectioning, respectively, with X-rays and laser light. MicroCT can be used on fresh or fixed tissue samples and is primarily designed to image bone rather than soft tissues. It requires complex back-projection algorithms to produce a two-dimensional image, and it is an expensive instrument. sTSLIM requires that a specimen be chemically fix...
Source: Journal of Histochemistry and Cytochemistry - April 24, 2013 Category: Biochemistry Authors: Buytaert, J. A. N., Johnson, S. B., Dierick, M., Salih, W. H. M., Santi, P. A. Tags: Articles Source Type: research

Histoenzymatic and Morphometric Analysis of Muscle Fiber Type Transformation during the Postnatal Development of the Chronically Food-Deprived Rat
We analyze the effect of chronic undernourishment on extensor digitorum longus (EDL) muscle maturation in the rat. Cytochrome c oxidase (COX) and alkaline ATPase histoenzymatic techniques were used to determine the relative proportion of different fiber types (oxidative/glycolytic and type I, IIa/IId, or IIb, respectively) and their cross-sectional area in control and undernourished EDL muscles at several postnatal (PN) ages. From PN days 15 to 45, undernourished EDL muscles showed predominance of oxidative and type IIa/IId fibers, but from PN days 60 to 90, there were a larger proportion of oxidative fibers and an equal p...
Source: Journal of Histochemistry and Cytochemistry - April 24, 2013 Category: Biochemistry Authors: Ruiz-Rosado, A., Fernandez-Valverde, F., Mariscal-Tovar, S., Hinojosa-Rodriguez, C. X., Hernandez-Valencia, J. A., Anzueto-Rios, A., Guadarrama-Olmos, J. C., Segura-Alegria, B., Jimenez-Estrada, I. Tags: Articles Source Type: research

Quantitative Histological Assessment of Xenobiotic-Induced Liver Enzyme Induction and Pituitary-Thyroid Axis Stimulation in Rats Using Whole-Slide Automated Image Analysis
Preclinical evaluation of a new compound, RO2910, identified a hypertrophic response in liver, thyroid gland, and pituitary gland (pars distalis). We aimed to develop and validate automated image analysis methods to quantify and refine the interpretation of semi-quantitative histology. Wistar-Han rats were administered RO2910 for 14 days. Liver, thyroid, and pituitary gland tissues were processed for routine histology and immunolabeled with anti–thyroid stimulating hormone (TSH) antibody (pituitary) and anti–topoisomerase II antibody (thyroid). Glass slides were scanned, image analysis methods were developed an...
Source: Journal of Histochemistry and Cytochemistry - April 24, 2013 Category: Biochemistry Authors: Garrido, R., Zabka, T. S., Tao, J., Fielden, M., Fretland, A., Albassam, M. Tags: Articles Source Type: research

Expression Patterns Suggest that Despite Considerable Functional Redundancy, Galectin-4 and -6 Play Distinct Roles in Normal and Damaged Mouse Digestive Tract
The galectin-4 protein is mostly expressed in the digestive tract and is associated with lipid raft stabilization, protein apical trafficking, wound healing, and inflammation. While most mammalian species, including humans, have a single Lgals4 gene, some mice have two paralogues: Lgals4 and Lgals6. So far, their significant similarities have hindered the analysis of their respective expression and function. We took advantage of two antibodies that discriminate between the galectin-4 and galectin-6 proteins to document their patterns of expression in the normal and the dextran sodium sulfate (DSS)-damaged digestive tract i...
Source: Journal of Histochemistry and Cytochemistry - April 24, 2013 Category: Biochemistry Authors: Houzelstein, D., Reyes-Gomez, E., Maurer, M., Netter, P., Higuet, D. Tags: Articles Source Type: research

Altered Expression of Endogenous Soluble Vascular Endothelial Growth Factor Receptor-2 Is Involved in the Progression of Esophageal Squamous Cell Carcinoma
Endogenous soluble vascular endothelial growth factor receptor-2 (esVEGFR-2), a new splicing variant of VEGFR-2, was shown to be the first endogenous specific inhibitor of lymphatic vessel growth. The expression of esVEGFR-2 and its clinicopathological roles in esophageal squamous cell carcinoma (ESCC) are unclear. In this article, quantitative RT-PCR was employed to detect the mRNA levels of esVEGFR-2 and VEGF-C in 90 paired primary ESCC tissues, along with immunohistochemical staining to measure esVEGFR-2 protein in 182 ESCC primary tissues. Correlations between esVEGFR-2 expression and clinicopathological features were ...
Source: Journal of Histochemistry and Cytochemistry - April 24, 2013 Category: Biochemistry Authors: Wu, Z.-Y., Chen, T., Zhao, Q., Huang, J.-H., Chen, J.-X., Zheng, C.-P., Xu, X.-E., Wu, J.-Y., Xu, L.-Y., Li, E.-M. Tags: Articles Source Type: research

Elevated BUBR1 Expression Is Associated with Poor Survival in Early Breast Cancer Patients: 15-Year Follow-up Analysis
This study was aimed at examining the prognostic value linked to the expression of BUBR1 in a group of patients with breast cancer. We analyzed the expression of BUBR1 in 98 stage II breast cancer patients with a median follow-up of 15 years. Immunohistochemical reactions were performed using monoclonal antibodies against BUBR1. We also studied the prognostic value of BUBR1 mRNA expression using the Kaplan-Meier (KM) plotter, which assessed the effect of 22,277 genes on survival in 2422 breast cancer patients. A background database was established using gene expression data and survival information on 2422 patients downloa...
Source: Journal of Histochemistry and Cytochemistry - April 24, 2013 Category: Biochemistry Authors: Maciejczyk, A., Szelachowska, J., Czapiga, B., Matkowski, R., Halon, A., Gyorffy, B., Surowiak, P. Tags: Articles Source Type: research

Developmental Localization of Nephrin in Zebrafish and Medaka Pronephric Glomerulus
In this study, we examined the developmental localization of Nephrin, an essential component of SD, in the pronephric glomerulus of zebrafish and medaka. In the mature glomerulus of both fish, Nephrin is localized along the glomerular basement membrane as seen in mammals, indicating that Nephrin is localized at the SD. Interestingly, Nephrin was detected already in immature podocytes before the SD and foot processes started to form in both fish. Nephrin was localized along the cell surface of immature podocytes but as different localization patterns. In zebrafish, Nephrin signal bordered the lateral membrane of podocytes, ...
Source: Journal of Histochemistry and Cytochemistry - March 25, 2013 Category: Biochemistry Authors: Ichimura, K., Fukuyo, Y., Nakamura, T., Powell, R., Sakai, T., Janknecht, R., Obara, T. Tags: Articles Source Type: research

First Molecular Cytogenetic High Resolution Characterization of the NIH 3T3 Cell Line by Murine Multicolor Banding
Since being established in 1963, the murine fibroblast cell line NIH 3T3 has been used in thousands of studies. NIH 3T3 immortalized spontaneously and became tetraploid shortly after its establishment. Here we report the first molecular cytogenetic characterization of NIH 3T3 using fluorescence in situ hybridization based multicolor banding (mcb). Overall, a complex rearranged karyotype presenting 16 breakpoints was characterized. Also it was possible to deduce the resulting gains and losses of copy numbers in NIH 3T3. Overall, only 1.8% of the NIH 3T3 genome is disome, 26.2% tri-, 60% tetra-, 10.8% quinta-, and 1.2% hexas...
Source: Journal of Histochemistry and Cytochemistry - March 25, 2013 Category: Biochemistry Authors: Leibiger, C., Kosyakova, N., Mkrtchyan, H., Glei, M., Trifonov, V., Liehr, T. Tags: Articles Source Type: research

Distinct Claudin Expression Profiles of Hepatocellular Carcinoma and Metastatic Colorectal and Pancreatic Carcinomas
In conclusion, HCC and both CRLM and PLM display distinct claudin expression profiles, which might provide better understanding of the pathobiology of these lesions and might be used for differential diagnosis. (Source: Journal of Histochemistry and Cytochemistry)
Source: Journal of Histochemistry and Cytochemistry - March 25, 2013 Category: Biochemistry Authors: Holczbauer, A., Gyongyosi, B., Lotz, G., Szijarto, A., Kupcsulik, P., Schaff, Z., Kiss, A. Tags: Articles Source Type: research

A Computational Approach to Detect Gap Junction Plaques and Associate Them with Cells in Fluorescent Images
Intercellular signaling is a fundamental requirement for complex biological system function and survival. Communication between adjoining cells is largely achieved via gap junction channels made up of multiple subunits of connexin proteins, each with unique selectivity and regulatory properties. Intercellular communication via gap junction channels facilitates transmission of an array of cellular signals, including ions, macromolecules, and metabolites that coordinate physiological processes throughout tissues and entire organisms. Although current methods used to quantify connexin expression rely on number or area density...
Source: Journal of Histochemistry and Cytochemistry - March 25, 2013 Category: Biochemistry Authors: Goldberg, J. S., Vadakkan, T. J., Hirschi, K. K., Dickinson, M. E. Tags: Articles Source Type: research