Journal of Histochemistry and Cytochemistry This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

A Historical Perspective on the Identification of Cell Types in Pancreatic Islets of Langerhans by Staining and Histochemical Techniques
Before the middle of the previous century, cell types of the pancreatic islets of Langerhans were identified primarily on the basis of their color reactions with histological dyes. At that time, the chemical basis for the staining properties of islet cells in relation to the identity, chemistry and structure of their hormones was not fully understood. Nevertheless, the definitive islet cell types that secrete glucagon, insulin, and somatostatin (A, B, and D cells, respectively) could reliably be differentiated from each other with staining protocols that involved variations of one or more tinctorial techniques, such as the...
Source: Journal of Histochemistry and Cytochemistry - July 27, 2015 Category: Biochemistry Authors: Baskin, D. G. Tags: Perspective Source Type: research

Histochemical Insights into Pancreatic Islet Biology
(Source: Journal of Histochemistry and Cytochemistry)
Source: Journal of Histochemistry and Cytochemistry - July 27, 2015 Category: Biochemistry Authors: Hull, R. L., Baskin, D. G. Tags: Editorial Source Type: research

Expression of Matrix Metalloproteinase (MMP)-20 and Potential Interaction with Dentin Sialophosphoprotein (DSPP) in Human Major Salivary Glands
This report reinforces our earlier suggestion that the SIBLING–MMP complexes may be involved in the turnover of extracellular proteins damaged by oxidation byproducts in metabolically active duct epithelial systems. (Source: Journal of Histochemistry and Cytochemistry)
Source: Journal of Histochemistry and Cytochemistry - June 25, 2015 Category: Biochemistry Authors: Koli, K., Saxena, G., Ogbureke, K. U. E. Tags: Articles Source Type: research

Expression of Thyroid Hormone Receptors in Villous Trophoblasts and Decidual Tissue at Protein and mRNA Levels Is Downregulated in Spontaneous and Recurrent Miscarriages
Thyroid hormones are essential for the maintenance of pregnancy, and a deficiency in maternal thyroid hormones has been associated with early pregnancy losses. The expression of THRα1, THRβ1 and THRα2 increases with gestational age. The aim of this study was the investigation of the protein and mRNA-levels of THR isoforms THRα1, THRα2, THRβ1 and THRβ2 in normal, spontaneous and recurrent miscarriages. The identification of THR-expressing cells in the decidua was done with double immunofluorescence. The nuclear expression of THRα1, THRα2, THRβ1 and THRβ2 is downre...
Source: Journal of Histochemistry and Cytochemistry - June 25, 2015 Category: Biochemistry Authors: Ziegelmuller, B., Vattai, A., Kost, B., Kuhn, C., Hofmann, S., Bayer, B., Toth, B., Jeschke, U., Ditsch, N. Tags: Articles Source Type: research

Temporal Heterogeneity Metrics in Apoptosis Induced by Anticancer Drugs
The apoptotic process is highly heterogeneous and asynchronous. A long-standing question is how many parameters define the time and reversibility of the apoptotic response at a single-cell level. We characterized at the single-cell and population levels the time sequence of apoptotic events in response to anti-cancer drugs using extrinsic and intrinsic apoptotic stimuli. We show that the temporal sequence of major apoptotic events is the same in response to all anti-cancer drugs studied: the apoptotic volume decrease and Na+ influx occur rapidly and are tightly coordinated with mitochondrial outer membrane depolarization (...
Source: Journal of Histochemistry and Cytochemistry - June 25, 2015 Category: Biochemistry Authors: Vorobjev, I., Barteneva, N. S. Tags: Articles Source Type: research

CD133+ and Nestin+ Glioma Stem-Like Cells Reside Around CD31+ Arterioles in Niches that Express SDF-1{alpha}, CXCR4, Osteopontin and Cathepsin K
Poor survival of high-grade glioma is at least partly caused by glioma stem-like cells (GSLCs) that are resistant to therapy. GSLCs reside in niches in close vicinity of endothelium. The aim of the present study was to characterize proteins that may be functional in the GSLC niche by performing immunohistochemistry on serial cryostat sections of human high-grade glioma samples. We have found nine niches in five out of five high-grade glioma samples that were all surrounding arterioles with CD31+ endothelial cells and containing cellular structures that were CD133+ and nestin+. All nine niches expressed stromal-derived fact...
Source: Journal of Histochemistry and Cytochemistry - June 25, 2015 Category: Biochemistry Authors: Hira, V. V. V., Ploegmakers, K. J., Grevers, F., Verbovšek, U., Silvestre-Roig, C., Aronica, E., Tigchelaar, W., Turnšek, T. L., Molenaar, R. J., Van Noorden, C. J. F. Tags: Articles Source Type: research

Wnt Signaling Cascades and the Roles of Syndecan Proteoglycans
Wnt signaling comprises a group of pathways emanating from the extracellular environment through cell-surface receptors into the intracellular milieu. Wnt signaling cascades can be divided into two main branches, the canonical/β-catenin pathway and the non-canonical pathways containing the Wnt/planar cell polarity and Wnt/calcium signaling. Syndecans are type I transmembrane proteoglycans with a long evolutionary history, being expressed in all Bilateria and in almost all cell types. Both Wnt pathways have been extensively studied over the past 30 years and shown to have roles during development and in a multitude of ...
Source: Journal of Histochemistry and Cytochemistry - June 25, 2015 Category: Biochemistry Authors: Pataki, C. A., Couchman, J. R., Brabek, J. Tags: Review Source Type: research

Maintaining Specimen Integrity for G6PD Screening by Cytofluorometric Assays
Cytochemical staining remains an efficient way of identifying females who are heterozygous for the X chromosome-linked glucose-6-phosphate dehydrogenase (G6PD) gene. G6PD is highly polymorphic with certain alleles resulting in low intracellular G6PD activity in red blood cells. Low intracellular G6PD activity is associated with a risk of severe hemolysis when exposed to an oxidative stress such as fava beans, certain drugs and infections. Heterozygous females express the enzyme from both X-chromosome alleles resulting in two red blood cell populations each with G6PD enzyme characteristics representative of each allele; for...
Source: Journal of Histochemistry and Cytochemistry - May 25, 2015 Category: Biochemistry Authors: Kahn, M., Ward, W. H. J., LaRue, N., Kalnoky, M., Pal, S., Domingo, G. J. Tags: Letters to the Editor Source Type: research

Vascular Expression of Transient Receptor Potential Vanilloid 1 (TRPV1)
(Source: Journal of Histochemistry and Cytochemistry)
Source: Journal of Histochemistry and Cytochemistry - May 25, 2015 Category: Biochemistry Authors: Sand, C. A., Grant, A. D., Nandi, M. Tags: Letters to the Editor Source Type: research

Correlation between Fibrillin-1 Degradation and mRNA Downregulation and Myofibroblast Differentiation in Cultured Human Dental Pulp Tissue
Myofibroblasts and extracellular matrix are important components in wound healing. Alpha-smooth muscle actin (α-SMA) is a marker of myofibroblasts. Fibrillin-1 is a major constituent of microfibrils and an extracellular-regulator of TGF-β1, an important cytokine in the transdifferentiation of resident fibroblasts into myofibroblasts. To study the correlation between changes in fibrillin-1 expression and myofibroblast differentiation, we examined alterations in fibrillin-1 and α-SMA expression in organotypic cultures of dental pulp in vitro. Extracted healthy human teeth were cut to 1-mm-thick slices and cu...
Source: Journal of Histochemistry and Cytochemistry - May 25, 2015 Category: Biochemistry Authors: Yoshiba, N., Yoshiba, K., Ohkura, N., Takei, E., Edanami, N., Oda, Y., Hosoya, A., Nakamura, H., Okiji, T. Tags: Articles Source Type: research

Structural Analysis of Alterations in Zebrafish Muscle Differentiation Induced by Simvastatin and Their Recovery with Cholesterol
In vitro studies show that cholesterol is essential to myogenesis. We have been using zebrafish to overcome the limitations of the in vitro approach and to study the sub-cellular structures and processes involved during myogenesis. We use simvastatin—a drug widely used to prevent high levels of cholesterol and cardiovascular disease—during zebrafish skeletal muscle formation. Simvastatin is an efficient inhibitor of cholesterol synthesis that has various myotoxic consequences. Here, we employed simvastatin concentrations that cause either mild or severe morphological disturbances to observe changes in the cytos...
Source: Journal of Histochemistry and Cytochemistry - May 25, 2015 Category: Biochemistry Authors: Campos, L. M., Rios, E. A., Midlej, V., Atella, G. C., Herculano-Houzel, S., Benchimol, M., Mermelstein, C., Costa, M. L. Tags: Articles Source Type: research

Metabotropic Glutamate Receptor 3 Is Downregulated and Its Expression Is Shifted From Neurons to Astrocytes in the Mouse Lateral Septum During the Postpartum Period
In this study, we used double fluorescence immunohistochemical approaches to characterize mGluR3 changes in LS of the postpartum brain. The number of mGluR3-immunoractive cells was significantly reduced in the dorsal (LSD) and intermediate (LSI) but not ventral (LSV) parts of the LS in postpartum versus virgin females. mGluR3 immunoreactivity in the LS was found predominantly in neurons (~70%), with a smaller portion (~20%–30%) in astrocytes. Colocalization analysis revealed a reduced mGluR3 expression in neurons but an increased astrocytic localization in postpartum LSI. This change in the pattern of expression sugg...
Source: Journal of Histochemistry and Cytochemistry - May 25, 2015 Category: Biochemistry Authors: Zhao, C., Gammie, S. C. Tags: Articles Source Type: research

Co-Expression of p16, Ki67 and COX-2 Is Associated with Basal Phenotype in High-Grade Ductal Carcinoma In Situ of the Breast
We assessed the co-expression of cell cycle-related biomarkers in a series of 121 consecutive cases of high-grade ductal carcinoma in situ (DCIS), pure or associated with invasive carcinoma, and their associations with the different immunoprofiles of DCIS. Cases were identified from the histopathology files of the Breast Pathology Laboratory, Federal University of Minas Gerais, Brazil, from 2003 to 2008. The expression of estrogen receptor, progesterone receptor, HER2 overexpression, cytokeratin 5, epidermal growth factor receptor 1, cyclooxygenase-2, p16 and Ki67 were assessed. Tumors were placed into five subgroups accor...
Source: Journal of Histochemistry and Cytochemistry - May 25, 2015 Category: Biochemistry Authors: Perez, A. A., Balabram, D., Rocha, R. M., da Silva Souza, A., Gobbi, H. Tags: Articles Source Type: research

High Phosphohistone H3 Expression Correlates with Adverse Clinical, Biological, and Pathological Factors in Neuroblastomas
In conclusion, high pHH3 expression correlates strongly with high MKI and MYCN amplification and indicates poor prognosis in neuroblastomas. (Source: Journal of Histochemistry and Cytochemistry)
Source: Journal of Histochemistry and Cytochemistry - May 25, 2015 Category: Biochemistry Authors: Ramani, P., Taylor, S., Miller, E., Sowa-Avugrah, E., May, M. T. Tags: Articles Source Type: research

Novel Cryptic Rearrangements in Adult B-Cell Precursor Acute Lymphoblastic Leukemia Involving the MLL Gene
MLL (mixed-lineage-leukemia) gene rearrangements are typical for acute leukemia and are associated with an aggressive course of disease, with a worse outcome than comparable case, and thus require intensified treatment. Here we describe a 69-year-old female with adult B cell precursor acute lymphoblastic leukemia (BCP-ALL) with hyperleukocytosis and immunophenotype CD10- and CD19+ with cryptic MLL rearrangements. G-banding at the time of diagnosis showed a normal karyotype: 46,XX. Molecular cytogenetics using multitude multicolor banding (mMCB) revealed a complex rearrangement of the two copies of chromosome 11. However, a...
Source: Journal of Histochemistry and Cytochemistry - April 25, 2015 Category: Biochemistry Authors: Othman, M. A. K., Grygalewicz, B., Pienkowska-Grela, B., Rincic, M., Rittscher, K., Melo, J. B., Carreira, I. M., Meyer, B., Marzena, W., Liehr, T. Tags: Articles Source Type: research