Journal of Histochemistry and Cytochemistry 
This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.
This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.How Vascular Endothelial Growth Factor-A (VEGF) Regulates Differentiation of Mesenchymal Stem Cells
Vascular endothelial growth factor A (VEGF), a key factor in angiogenesis, plays an essential role in skeletal development and postnatal homeostasis. VEGF serves as a survival factor for chondrocytes and couples the resorption of cartilage with bone formation during endochondral ossification. Recently, it has also been found to regulate the balance between osteoblast and adipocyte differentiation in bone marrow mesenchymal stem cells. Surprisingly, this regulatory function of VEGF is not based on paracrine signaling involving cell surface receptor activation. Instead, the mechanism appears to utilize intracellular VEGF, wh...
Source: Journal of Histochemistry and Cytochemistry - January 25, 2014 Category: Biochemistry Authors: Berendsen, A. D., Olsen, B. R. Tags: Perspective Source Type: research
Peroxiredoxin I and II in Human Eyes: Cellular Distribution and Association with Pterygium and DNA Damage
Peroxiredoxin I and II are both 2-Cys members of the peroxiredoxin family of antioxidant enzymes and inactivate hydrogen peroxide. On western blotting, both enzymes appeared as 22-kD proteins and were present in the sclera, retina and iris. Immunohistochemistry showed strong cytoplasmic labeling in the basal cells of the corneal epithelial layer and the corneoscleral limbus. The melanocytes within the stroma of the iris and the anterior epithelial cells of the lens also showed strong cytoplasmic labeling. The fibrous structure of the stroma and the posterior surface of the ciliary body were also labeled. There was also str...
Source: Journal of Histochemistry and Cytochemistry - December 26, 2013 Category: Biochemistry Authors: Klebe, S., Callahan, T., Power, J. H. Tags: Articles Source Type: research
Distribution of MT1 Melatonin Receptor Promoter-Driven RFP Expression in the Brains of BAC C3H/HeN Transgenic Mice
The pineal hormone melatonin activates two G-protein coupled receptors (MT1 and MT2) to regulate in part biological functions. The MT1 and MT2 melatonin receptors are heterogeneously distributed in the mammalian brain including humans. In the mouse, only a few reports have assessed the expression of the MT1 melatonin receptor expression using 2-iodomelatonin binding, in situ hybridization and/or polymerase chain reaction (PCR). Here, we described a transgenic mouse in which red fluorescence protein (RFP) is expressed under the control of the endogenous MT1 promoter, by inserting RFP cDNA at the start codon of MTNR1a gene w...
Source: Journal of Histochemistry and Cytochemistry - December 26, 2013 Category: Biochemistry Authors: Adamah-Biassi, E., Zhang, Y., Jung, H., Vissapragada, S., Miller, R., Dubocovich, M. Tags: Articles Source Type: research
Expression of Calcium-Binding Proteins in Layer 1 Reelin-Immunoreactive Cells during Rat and Mouse Neocortical Development
Cajal-Retzius cells in layer 1 of the developing cerebral cortex and their product of secretion, reelin, an extracellular matrix protein, play a crucial role in establishing the correct lamination pattern in this tissue. As many studies into reelin signaling routes and pathological alterations are conducted in murine models, we used double-labeling and confocal microscopy to compare the distribution of the cell-specific markers, calretinin and calbindin, in reelin-immunoreactive cells during postnatal rat and mouse neocortical development. In the rat, neither calretinin nor calbindin colocalized with reelin in Cajal-Retziu...
Source: Journal of Histochemistry and Cytochemistry - December 26, 2013 Category: Biochemistry Authors: Martinez-Galan, J. R., Moncho-Bogani, J., Caminos, E. Tags: Articles Source Type: research
Matriptase Expression and Zymogen Activation in Human Pilosebaceous Unit
Studies of human genetic disorders and mouse models reveal the important roles of matriptase in hair growth. Here, we investigate matriptase expression and zymogen activation in hair follicles. We show: 1) layer-dependent distribution patterns, with much higher matriptase expression in cells of the outer root sheath and matrix cells of the hair bulb than in cells of the inner root sheath; 2) cycle-dependent expression patterns, with matriptase expressed in the anagen and catagen phases of the hair lifecycle, but not in the telogen phase; 3) reduced expression of the matriptase inhibitor, HAI-1, in the catagen phase, sugges...
Source: Journal of Histochemistry and Cytochemistry - December 26, 2013 Category: Biochemistry Authors: Wu, B.-Y., Lee, S.-P., Hsiao, H.-C., Chiu, H., Chen, C.-Y., Yeo, Y. H., Lee, H.-S., Chen, Y.-W., Kaul, M., Kataoka, H., Johnson, M. D., Wang, J.-K., Lin, C.-Y. Tags: Articles Source Type: research
ADAMTS-4 and Biglycan are Expressed at High Levels and Co-Localize to Podosomes During Endothelial Cell Tubulogenesis In Vitro
We examined the expression of ADAMTS-1, -4, and -5 metalloproteinases and their proteoglycan substrates versican, decorin, and biglycan as human umbilical vein endothelial cells (HUVECs) formed tubes within type I collagen gels in vitro. Tubulogenic and control HUVEC cultures expressed low levels of ADAMTS-1 and -5 mRNAs, but ADAMTS-4 mRNA was relatively abundant and was significantly elevated (as was ADAMTS-4 protein) in tubulogenic cultures versus controls. Immunocytochemistry revealed ADAMTS-4 in f-actin- and cortactin-positive podosome-like puncta in single cells and mature tubes. Tubulogenic and control cultures expre...
Source: Journal of Histochemistry and Cytochemistry - December 26, 2013 Category: Biochemistry Authors: Obika, M., Vernon, R. B., Gooden, M. D., Braun, K. R., Chan, C. K., Wight, T. N. Tags: Articles Source Type: research
Histone Deacetylases: A Saga of Perturbed Acetylation Homeostasis in Cancer
In the current era of genomic medicine, diseases are identified as manifestations of anomalous patterns of gene expression. Cancer is the principal example among such maladies. Although remarkable progress has been achieved in the understanding of the molecular mechanisms involved in the genesis and progression of cancer, its epigenetic regulation, particularly histone deacetylation, demands further studies. Histone deacetylases (HDACs) are one of the key players in the gene expression regulation network in cancer because of their repressive role on tumor suppressor genes. Higher expression and function of deacetylases dis...
Source: Journal of Histochemistry and Cytochemistry - December 26, 2013 Category: Biochemistry Authors: Parbin, S., Kar, S., Shilpi, A., Sengupta, D., Deb, M., Rath, S. K., Patra, S. K. Tags: Articles Source Type: research
Peer Review and Reproducibility. Crisis or Time for Course Correction?
(Source: Journal of Histochemistry and Cytochemistry)
Source: Journal of Histochemistry and Cytochemistry - December 26, 2013 Category: Biochemistry Authors: Couchman, J. R. Tags: Editorial Source Type: research
Rapid Immunocytochemistry with Simple Heat-Induced Antigen Retrieval Technique for Improvement in the Quality of Cytological Diagnosis
In conclusion, we established a rapid ICC procedure using a simple HIAR method, which allowed efficient immunostaining of a panel of antigens, including nuclear antigens, within only 11 min. The combined use of this rapid ICC technique with other staining techniques could be useful for improving intraoperative cytological diagnoses. (Source: Journal of Histochemistry and Cytochemistry)
Source: Journal of Histochemistry and Cytochemistry - November 25, 2013 Category: Biochemistry Authors: Denda, T., Kamoshida, S., Kawamura, J., Harada, K., Kawai, K., Kuwao, S., Sawabe, M. Tags: Articles Source Type: research
Sphingomyelin Patches on Pancreatic Beta-cells Are Indicative of Insulin Secretory Capacity
The establishment and validation of specific markers on the surfaces of pancreatic beta-cells would have a significant impact on the development of agents that specifically target these cells for imaging and/or image-guided therapy in diabetes patient samples. We have recently described unique, cholesterol-stabilized sphingomyelin (SM) patches on the surfaces of beta-cells using the IC2 antibody. To further investigate the utility of SM patches as a unique beta-cell biomarker, we embarked on the current study to correlate the expression of this antigen with the insulin secretory capacity of beta-cells in tissue samples fro...
Source: Journal of Histochemistry and Cytochemistry - November 25, 2013 Category: Biochemistry Authors: Kavishwar, A., Moore, A. Tags: Articles Source Type: research
Expression of Asialoglycoprotein Receptor 1 in Human Hepatocellular Carcinoma
We examined ASGPR1 expression levels by immunohistochemistry in HCC with different grades. Guidance for a targeting delivery strategy for anti-cancer drugs to HCC is suggested in this report. (Source: Journal of Histochemistry and Cytochemistry)
Source: Journal of Histochemistry and Cytochemistry - November 25, 2013 Category: Biochemistry Authors: Shi, B., Abrams, M., Sepp-Lorenzino, L. Tags: Articles Source Type: research
The Compact Mutation of Myostatin Causes a Glycolytic Shift in the Phenotype of Fast Skeletal Muscles
Myostatin is an important negative regulator of skeletal muscle growth. The hypermuscular Compact (Cmpt) mice carry a 12-bp natural mutation in the myostatin propeptide, with additional modifier genes being responsible for the phenotype. Muscle cellularity of the fast-type tibialis anterior (TA) and extensor digitorum longus (EDL) as well as the mixed-type soleus (SOL) muscles of Cmpt and wild-type mice was examined by immunohistochemical staining of the myosin heavy chain (MHC) proteins. In addition, transcript levels of MHC isoforms were quantified by qPCR. Based on our results, all investigated muscles of Cmpt mice were...
Source: Journal of Histochemistry and Cytochemistry - November 25, 2013 Category: Biochemistry Authors: Baan, J. A., Kocsis, T., Keller-Pinter, A., Muller, G., Zador, E., Dux, L., Mendler, L. Tags: Articles Source Type: research
Claudin 1 Expression Characterizes Human Uterine Cervical Reserve Cells
Stem cells participate in cervical carcinogenesis but their function and exact features are still not clear. One type of stem-like cells are endocervical reserve cells (RCs), and their association with other normal/altered cervical cells is not exactly known. Epithelial cells are attached to each other by tight junctions. Their dominant components are the claudin proteins, which show changed expression in cancer; however, no data are available on their pattern. Expressions of various claudins (1, 2, 3, 4, 7), occludin, cytokeratins 5/6 and 7, and p63 were analyzed in 60 paraffin-embedded cervical samples. Immunohistochemic...
Source: Journal of Histochemistry and Cytochemistry - November 25, 2013 Category: Biochemistry Authors: Zinner, B., Gyongyosi, B., Babarczi, E., Kiss, A., Sobel, G. Tags: Articles Source Type: research
Neuron-derived IgG Protects Neurons from Complement-dependent Cytotoxicity
In this study, we confirm IgG production by rat cortical neurons at the protein and mRNA levels, with 69.0 ± 5.8% of cortical neurons IgG-positive. Injury to primary-culture neurons was induced by complement leading to increases in IgG production. Blockage of neuron-derived IgG resulted in more neuronal death and early apoptosis in the presence of complement. In addition, FcRI was found in microglia and astrocytes. Expression of FcR I in microglia was increased by exposure to neuron-derived IgG. Release of NO from microglia triggered by complement was attenuated by neuron-derived IgG, and this attenuation could be r...
Source: Journal of Histochemistry and Cytochemistry - November 25, 2013 Category: Biochemistry Authors: Zhang, J., Niu, N., Li, B., McNutt, M. A. Tags: Articles Source Type: research
Lipoprotein Lipase (LPL) is Associated with Neurite Pathology and Its Levels Are Markedly Reduced in the Dentate Gyrus of Alzheimer's Disease Brains
Lipoprotein lipase (LPL) is involved in regulation of fatty acid metabolism, and facilitates cellular uptake of lipoproteins, lipids and lipid-soluble vitamins. We evaluated LPL distribution in healthy and Alzheimer’s disease (AD) brain tissue and its relative levels in cerebrospinal fluid. LPL immunostaining is widely present in different neuronal subgroups, microglia, astrocytes and oligodendroglia throughout cerebrum, cerebellum and spinal cord. LPL immunoreactivity is also present in leptomeninges, small blood vessels, choroid plexus and ependymal cells, Schwann cells associated with cranial nerves, and in anteri...
Source: Journal of Histochemistry and Cytochemistry - November 25, 2013 Category: Biochemistry Authors: Gong, H., Dong, W., Rostad, S. W., Marcovina, S. M., Albers, J. J., Brunzell, J. D., Vuletic, S. Tags: Articles Source Type: research
