CEACAM2-L on Spermatids Interacts with Poliovirus Receptor on Sertoli Cells in Mouse Seminiferous Epithelium

In this study, we investigated the interaction between CEACAM2-L and Poliovirus receptor (PVR), which is also from the IgSF and is expressed by Sertoli cells. Immunohistochemical analysis showed that CEACAM2-L expressed on elongated spermatids was in close contact with PVR-positive cell processes of Sertoli cells. Immunoprecipitation experiments both in vivo and in vitro demonstrated a direct heterophilic interaction between CEACAM2-L and PVR. We show that the N-terminal Ig domain of CEACAM2-L was critical for its interaction with PVR. In addition, we found that CEACAM2-L formed heterophilic trans-tetramers with PVR in transfected COS-7 cells. From these data, we propose that Sertoli cells recognize the excess cytoplasm of elongated spermatids through the PVR-CEACAM2-L interaction in mouse testis.

Source: Journal of Histochemistry and Cytochemistry - August 25, 2014 Category: Biochemistry Authors: Salaheldeen, E., Howida, A., Wakayama, T., Iida, H. Tags: Articles Source Type: research