Erythritol production by yeasts: a snapshot of current knowledge
Abstract
Erythritol is a four‐carbon sugar alcohol produced by microorganisms as an osmoprotectant. It could be used as a natural sweetener in the pharmaceutical and food industries. Here, a snapshot on current knowledge on erythritol metabolism and synthesis, optimization of its production, more precisely process and producer strain improvement is presented. (Source: Yeast)
Source: Yeast - December 1, 2017 Category: Molecular Biology Authors: Fr édéric Carly, Patrick Fickers Tags: REVIEW Source Type: research
Misidentification of genome assemblies in public databases: the case of Naumovozyma dairenensis and proposal of a protocol to correct misidentifications
Abstract
Online sequence databases such as NCBI GenBank serve as a tremendously useful platform for researchers to share and reuse published data. However, submission systems lack control for errors such as organism misidentification, which once entered in the database can be propagated and mislead downstream analyses. Here we present an illustrating case of misidentification of Candida albicans from a clinical sample as Naumovozyma dairenensis based on whole‐genome shotgun data. Analyses of phylogenetic markers, read mapping and single nucleotide polymorphisms (SNP) served to correct the identification. We propose that ...
Source: Yeast - December 1, 2017 Category: Molecular Biology Authors: Aimilia A. Stavrou, Ver ónica Mixão, Teun Boekhout, Toni Gabaldón Tags: RESEARCH ARTICLE Source Type: research
Identification and characterization of novel promoters for recombinant protein production in yeast Pichia pastoris
Abstract
Pichia pastoris expression system has been widely used in recombinant protein production. So far the majority of heterologous proteins are expressed by methanol inducible promoter PAOX1 and constitutive promoter PGAP. The use of other promoters is rather limited. Here we selected 16 potentially efficient and regulatory promoter candidates based on the RNA‐seq and RNA folding free energy ΔG data. GFP and recombinant amylase were inserted after these promoters to reveal their strength and efficiency under different carbon sources and culture scales. Two novel promoters were successfully identified and could possi...
Source: Yeast - December 1, 2017 Category: Molecular Biology Authors: Ning Xu, Jinxiang Zhu, Qiaoyun Zhu, Yanzi Xing, Menghao Cai, Tianyi Jiang, Mian Zhou, Yuanxing Zhang Tags: RESEARCH ARTICLE Source Type: research
A history of genome editing in Saccharomyces cerevisiae
Abstract
Genome editing is a form of highly precise genetic engineering which produces alterations to an organism's genome as small as a single base pair with no incidental or auxiliary modifications; this technique is crucial to the field of synthetic biology, which requires such precision in the installation of novel genetic circuits into host genomes. While a new methodology for most organisms, genome editing capabilities have been used in the budding yeast Saccharomyces cerevisiae for decades. In this review, I will present a brief history of genome editing in S. cerevisiae, discuss the current gold standard method of ...
Source: Yeast - December 1, 2017 Category: Molecular Biology Authors: William G. Alexander Tags: BUDDING TOPIC Source Type: research
PER1, GUP1 and CWH43 of methylotrophic yeast Ogataea minuta are involved in cell wall integrity
Abstract
In eukaryotes, the glycosylphosphatidylinositol (GPI) modification of many glycoproteins on the cell surface is highly conserved. The lipid moieties of GPI‐anchored proteins undergo remodelling processes during their maturation. To date, the products of the PER1, GUP1 and CWH43 genes of the yeast Saccharomyces cerevisiae have been shown to be involved in the lipid remodelling. Here, we focus on the putative GPI remodelling pathway in the methylotrophic yeast Ogataea minuta. We found that the O. minuta homologues of PER1, GUP1 and CWH43 are functionally compatible with those of S. cerevisiae. Disruption of GUP1 o...
Source: Yeast - November 29, 2017 Category: Molecular Biology Authors: Xin ‐Xin Xu, Akiko Komatsuzaki, Yasunori Chiba, Xiao‐Dong Gao, Takehiko Yoko‐o Tags: RESEARCH ARTICLE Source Type: research
On the catabolism of amino acids in the yeast Dekkera bruxellensis and the implications for industrial fermentation processes
Abstract
In the last years several reports have reported the capacity of the yeast Dekkera (Brettanomyces) bruxellensis to survive and adapt to the industrial process of alcoholic fermentation. Much of this feature seems to relate to the ability to assimilate limiting sources of nutrients, or somehow some that are inaccessible to Saccharomyces cerevisiae, in particular the sources of nitrogen. Among them, amino acids (AA) are relevant in terms of beverage musts, and could also be important for bioethanol. In view of the limited knowledge on the control of AA, the present work combines physiological and genetic studies to u...
Source: Yeast - November 29, 2017 Category: Molecular Biology Authors: Denise Castro Parente, Danielli Batista Bezerra Cajueiro, Irina Charlot Pe ña Moreno, Fernanda Cristina Bezerra Leite, Will De Barros Pita, Marcos Antonio De Morais Tags: RESEARCH ARTICLE Source Type: research
Melatonin improves survival and respiratory activity of yeast cells challenged by alpha ‐synuclein and menadione
Yeast,Volume 35, Issue 3, Page 281-290, March 2018. (Source: Yeast)
Source: Yeast - November 16, 2017 Category: Molecular Biology Source Type: research
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Yeast,Volume 35, Issue 3, Page 281-290, March 2018. (Source: Yeast)
Source: Yeast - November 16, 2017 Category: Molecular Biology Source Type: research
Influence of phosphatidylserine and phosphatidylethanolamine on farnesol tolerance in Candida albicans
In this study, we found that loss of enzymes that synthesize the phospholipids phosphatidylserine (PS) and/or phosphatidylethanolamine (PE) compromise the tolerance of C. albicans to farnesol. Compared to wild‐type, the phospholipid mutant cho1∆/∆ (loss of PS and decreased PE synthesis) shows greater inhibition of growth, loss of ATP production, increased consumption of oxygen, and increased formation of reactive oxygen species (ROS) in the presence of farnesol (FOH). The cho1∆/∆ mutant also exhibits decreased sensitivity to mitochondrial ATPase inhibition, suggesting that cells lacking PS and/or downstream PE re...
Source: Yeast - November 16, 2017 Category: Molecular Biology Authors: Sahar Hasim, Elyse N. Vaughn, Dallas Donohoe, Donna M. Gordon, Susan Pfiffner, Todd B. Reynolds Tags: RESEARCH ARTICLE Source Type: research
Saccharomyces interspecies hybrids as model organisms for studying yeast adaptation to stressful environments
Abstract
The strong development of molecular biology techniques and next‐generation sequencing technologies in the last two decades has significantly improved our understanding of the evolutionary history of Saccharomyces yeasts. It has been shown that many strains isolated from man‐made environments are not pure genetic lines, but contain genetic materials from different species that substantially increase their genome complexity. A number of strains have been described as interspecies hybrids, implying different yeast species that under specific circumstances exchange and recombine their genomes. Such fusing usually ...
Source: Yeast - November 13, 2017 Category: Molecular Biology Authors: Ksenija Lopandic Tags: Special Issue ‐ Yeast interspecies hybrids Source Type: research
Issue Information ‐ Notes for Contribs
No abstract is available for this article. (Source: Yeast)
Source: Yeast - November 7, 2017 Category: Molecular Biology Tags: Issue Information ‐ Notes for Contribs Source Type: research
Issue Information
No abstract is available for this article. (Source: Yeast)
Source: Yeast - November 7, 2017 Category: Molecular Biology Tags: Issue Information Source Type: research
Understanding the metabolic burden of recombinant antibody production in Saccharomyces cerevisiae using a quantitative metabolomics approach
We present a comparison of metabolite profiles of a wild‐type strain with those of three strains producing recombinant antibody variants of increasing size and complexity: a scFv fragment, a scFv‐Fc fusion protein, and a full‐length IgG molecule. Under producing conditions, all three recombinant strains showed a clear decrease in growth rate compared to the wild‐type strain and the severity of the growth phenotype increased with size of the protein. The levels of 76 intracellular metabolites were determined using a targeted (semi) quantitative mass spectrometry based approach. Based on unsupervised and supervised m...
Source: Yeast - November 1, 2017 Category: Molecular Biology Authors: Jorg C. Ruijter, Essi V. Koskela, Jatin Nandania, Alexander D. Frey, Vidya Velagapudi Tags: RESEARCH ARTICLE Source Type: research
Melatonin improves survival and respiratory activity of yeast cells challenged by alpha ‐synuclein and menadione
In this study we propose to evaluate the respiratory outcome of yeast cells expressing α‐synuclein. Cell viability, or growth on selective media for respiratory activity was mainly affected in the α‐synuclein expressing cells if they were also treated with menadione, which stimulates ROS production. We also tested whether melatonin, a natural antioxidant, would counteract the deleterious effects of α‐synuclein and menadione. In fact, melatonin addition improved respiratory growth of α‐synuclein/menadione challenged cells, presented a general improvement in the enzymatic activity of the respiratory complexes, an...
Source: Yeast - November 1, 2017 Category: Molecular Biology Authors: Mariana A. Zampol, Mario H. Barros Tags: RESEARCH ARTICLE Source Type: research
Saccharomyces cerevisiae x Saccharomyces uvarum hybrids generated under different conditions share similar winemaking features
Abstract
Interspecific hybrids among species in the Saccharomyces genus are frequently detected in anthropic habitats and can also be obtained easily in the laboratory. This occurs because the most important genetic barriers among Saccharomyces species are post‐zygotic. Depending on several factors, including the involved strains, the hybridisation mechanism and stabilisation conditions, the hybrids that bear differential genomic constitutions, and hence phenotypic variability, can be obtained. In the present study, Saccharomyces cerevisiae x Saccharomyces uvarum hybrids were constructed using genetically and physiologic...
Source: Yeast - November 1, 2017 Category: Molecular Biology Authors: Origone Andrea Cecilia, Rodr íguez María Eugenia, Oteiza Juan Martín, Querol Amparo, Lopes Christian Ariel Tags: Special Issue ‐ Yeast interspecies hybrids Source Type: research
