Opi1 mediates repression of phospholipid biosynthesis by phosphate limitation in the yeast Saccharomyces cerevisiae
Abstract
Structural genes of phospholipid biosynthesis in the yeast S. cerevisiae are transcribed when precursor molecules inositol and choline (IC) are limiting. Gene expression is stimulated by the heterodimeric activator Ino2/Ino4 which binds to ICRE (inositol/choline‐responsive element) promoter sequences. Activation is prevented by repressor Opi1, counteracting Ino2 when high concentrations of IC are available. Here we show that ICRE‐dependent gene activation is not only repressed by an excess of IC but also under conditions of phosphate starvation. While PHO5 is activated by phosphate limitation, INO1 expression ...
Source: Yeast - September 30, 2016 Category: Molecular Biology Authors: Felix Kliewe, Jacqueline Kumme, Mathias Grigat, Stefan Hintze, Hans ‐Joachim Schüller Tags: Research Article Source Type: research
The regulatable MAL32 promoter in S. cerevisiae: characteristics and tools to facilitate its use
This article is protected by copyright. All rights reserved. (Source: Yeast)
Source: Yeast - September 30, 2016 Category: Molecular Biology Authors: Matthias Meurer, Veronika Chevyreva, Bram Cerulus, Michael Knop Tags: Research Article Source Type: research
ERG6 gene deletion modifies Kluyveromyces lactis susceptibility to various growth inhibitors
Abstract
The ERG6 gene encodes a S‐adenosylmethionine dependent sterol C‐24 methyltransferase in the ergosterol biosynthetic pathway. In this work we report the results of functional analysis of the Kluyveromyces lactis ERG6 gene. We cloned the KlERG6 gene, which was able to complement the erg6Δ mutation both in K. lactis and Saccharomyces cerevisiae. The lack of ergosterol in the Klerg6 deletion mutant was accompanied with increased expression of genes encoding last steps of ergosterol biosynthesis pathway as well as the KlPDR5 gene encoding an ABC transporter. The Klerg6Δ mutation resulted in reduced cell´s suscep...
Source: Yeast - September 25, 2016 Category: Molecular Biology Authors: Alexandra Konecna, Nora Toth Hervay, Martin Valachovic, Yvetta Gbelska Tags: Research Article Source Type: research
Development of stable haploid strains and molecular genetic tools for Naumovozyma castellii (Saccharomyces castellii)
Abstract
The budding yeast Naumovozyma castellii (syn. Saccharomyces castellii) has been included in comparative genomics studies, functional analyses of centromere DNA elements, and was shown to possess beneficial traits for telomere biology research. To provide useful tools for molecular genetic approaches, we produced stable haploid heterothallic strains from an early ancestral strain derived from the N. castellii collection strain CBS 4310. To this end, we deleted the gene encoding the Ho endonuclease, which is essential for the mating type switching. Gene replacement of HO with the kanMX3 resistance cassette was perfo...
Source: Yeast - September 25, 2016 Category: Molecular Biology Authors: Ahu Karademir Andersson, Stina Oredsson, Marita Cohn Tags: Research Article Source Type: research
Issue Information ‐ Notes for Contribs p1
No abstract is available for this article. (Source: Yeast)
Source: Yeast - September 4, 2016 Category: Molecular Biology Tags: Issue Information ‐ Notes for Contribs p1 Source Type: research
Issue Information ‐ Info Page
No abstract is available for this article. (Source: Yeast)
Source: Yeast - September 4, 2016 Category: Molecular Biology Tags: Issue Information ‐ Info Page Source Type: research
Issue Information ‐ TOC
No abstract is available for this article. (Source: Yeast)
Source: Yeast - September 4, 2016 Category: Molecular Biology Tags: Issue Information ‐ TOC Source Type: research
Proteomic and genomic characterization of a yeast model for Ogden syndrome
In conclusion, the data presented here further support the disruptive nature of the S37P/Ogden mutation and identify affected cellular processes potentially contributing to the severe phenotype seen in Ogden syndrome. Data are available via GEO under identifier GSE86482 or with ProteomeXchange under identifier PXD004923. (Source: Yeast)
Source: Yeast - August 31, 2016 Category: Molecular Biology Authors: Max J. D örfel, Han Fang, Jonathan Crain, Michael Klingener, Jake Weiser, Gholson J. Lyon Tags: Research Article Source Type: research
Natural gene expression variation studies in yeast
This article is protected by copyright. All rights reserved. (Source: Yeast)
Source: Yeast - August 31, 2016 Category: Molecular Biology Authors: Dawn A. Thompson, Francisco A. Cubillos Tags: Budding Topic Source Type: research
Use of ade1 & ade2 mutations for development of a versatile red/white color assay of amyloid ‐induced oxidative stress in Saccharomyces cerevisiae
Abstract
Mutations in adenine biosynthesis pathway genes ADE1 & ADE2 have been conventionally used to score for prion [PSI+] in yeast. If ade1‐14 mutant allele is present, which contains a premature stop codon, [psi‐] yeast appear red on YPD medium due to accumulation of a red intermediate compound in vacuoles. In [PSI+] yeast, partial inactivation of the translation termination factor, Sup35 protein, due to its amyloid aggregation allows for read‐through of the ade1‐14 stop codon and the yeast appears white as the red intermediate pigment is not accumulated. The red color development in ade1 & ade2 mutant ...
Source: Yeast - August 31, 2016 Category: Molecular Biology Authors: Vidhya Bharathi, Amandeep Girdhar, Archana Prasad, Meenkshi Verma, Vibha Taneja, Basant K Patel Tags: Research Article Source Type: research
Metschnikowia: half tetrads, a regicide, and the fountain of youth
Abstract
The purpose of this review is to introduce Metschnikowia to the yeast researcher community and to convince readers that the genus is a worthwhile object of study in developmental biology, genetics, ecology, and biotechnology. Metschnikowia sits at the foundation of modern immunology, having been instrumental in the discovery of animal phagocytosis. Some 81 species form a monophyletic group within the Metschnikowiaceae, which also include the smaller genus Clavispora and a few clades of Candida species. The family stands out by the habit of forming, by meiosis, only two ascospores, which in Metschnikowia are needle...
Source: Yeast - August 31, 2016 Category: Molecular Biology Authors: Marc ‐André Lachance Tags: Yeast Primer Source Type: research
Blastobotrys (Arxula) adeninivorans: a promising alternative yeast for biotechnology and basic research
Abstract
Blastobotrys adeninivorans (syn. Arxula adeninivorans) is a non‐conventional, non‐pathogenic, imperfect, haploid yeast, belonging to the subphylum Saccharomycotina, which has to date received comparatively little attention from researchers. It possesses unusual properties such as thermo‐ and osmotolerance, and a broad substrate spectrum. Depending on the cultivation temperature B. (A.) adeninivorans exhibits different morphological forms and various post‐translational modifications and protein expression properties that are strongly correlated with the morphology. The genome has been completely sequenced a...
Source: Yeast - August 24, 2016 Category: Molecular Biology Authors: Anna Malak, Kim Baronian, Gotthard Kunze Tags: Yeast Primer Source Type: research
Issue Information ‐ Notes for Contribs p1
No abstract is available for this article. (Source: Yeast)
Source: Yeast - August 10, 2016 Category: Molecular Biology Tags: Issue Information ‐ Notes for Contribs p1 Source Type: research
Evaluation of antimicrobial activity and probiotic properties of wild ‐strain Pichia kudriavzevii isolated from frozen idli batter
Abstract
The present research was undertaken to study the probiotic characteristics of Pichia kudriavzevii isolated from frozen idli batter. Polymerase chain reaction amplification with 18S rRNA primers confirmed Pichia kudriavzevii, a xylose‐utilizing probiotic strain. It was resistant to physiological concentrations of bile salts, pepsin and pancreatic enzyme. It also showed efficient auto‐aggregation as well as co‐aggregation ability with four commercial probiotic yeasts and exhibited good hydrophobicity in xylene and toluene. The strain inhibited the growth of 13 enteropathogens and showed a commensal relationshi...
Source: Yeast - August 10, 2016 Category: Molecular Biology Authors: Ramachandran Chelliah, Sudha Rani Ramakrishnan, Prince R. Prabhu, Usha Antony Tags: ISSY32 Special Issue Source Type: research
Issue Information ‐ Info Page
No abstract is available for this article. (Source: Yeast)
Source: Yeast - August 10, 2016 Category: Molecular Biology Tags: Issue Information ‐ Info Page Source Type: research
