Issue Information
No abstract is available for this article. (Source: Yeast)
Source: Yeast - July 7, 2017 Category: Molecular Biology Tags: Issue Information Source Type: research
Division of labour in the yeast, Saccharomyces cerevisiae
Abstract
Division of labour between different specialized cell types is a central part of how we describe complexity in multicellular organisms. However, it is increasingly being recognised that division of labour also plays an important role in the lives of predominantly unicellular organisms. Saccharomyces cerevisiae displays several phenotypes that could be considered a division of labour, including quiescence, apoptosis and biofilm formation, but they have not been explicitly treated as such. We discuss each of these examples, using a definition of division of labour that involves phenotypic variation between cells wit...
Source: Yeast - July 5, 2017 Category: Molecular Biology Authors: Dominika M. Wloch ‐Salamon, Roberta M. Fisher, Birgitte Regenberg Tags: Budding Topic Source Type: research
Alternative Saccharomyces interspecies hybrid combinations and their potential for low ‐temperature wort fermentation
Abstract
The lager yeast hybrid (S. cerevisiae x S. eubayanus) possesses two key characteristics that are essential for lager brewing: efficient sugar utilization and cold tolerance. Here we explore the possibility that the lager yeast phenotype can be recreated by hybridizing S. cerevisiae ale yeast with a number of cold tolerant Saccharomyces species including S. arboricola, S. eubayanus, S. mikatae and S. uvarum. Interspecies hybrids performed better than parental strains in lager brewing conditions (12 °C and 12 °P wort), with the S. mikatae hybrid performing as well as the S. eubayanus hybrid. Where the S. cerevisia...
Source: Yeast - July 1, 2017 Category: Molecular Biology Authors: Jarkko Nikulin, Kristoffer Krogerus, Brian Gibson Tags: Special Issue ‐ Yeast interspecies hybrids Source Type: research
Genome ‐wide map of Apn1 binding sites under oxidative stress in Saccharomyces cerevisiae
Abstract
The DNA is cells is continuously exposed to reactive oxygen species resulting in toxic and mutagenic DNA damage. Although the repair of oxidative DNA damage occurs primarily through the base excision repair (BER) pathway, the nucleotide excision repair (NER) pathway processes some of the same lesions. In addition, damage tolerance mechanisms, such as recombination and translesion synthesis, enable cells to tolerate oxidative DNA damage, especially when BER and NER capacities are exceeded. Thus, disruption of BER alone or disruption of BER and NER in Saccharomyces cerevisiae leads to increased mutations as well as ...
Source: Yeast - July 1, 2017 Category: Molecular Biology Authors: Lydia P. Morris, Andrew B. Conley, Natalya Degtyareva, I. King Jordan, Paul W. Doetsch Tags: Research Article Source Type: research
ß‐1,6‐glucan synthesis‐associated genes are required for proper spore wall formation in Saccharomyces cerevisiae
Abstract
The yeast spore wall is an excellent model to study the assembly of an extracellular macromolecule structure. In the present study, mutants defective in ß‐1,6‐glucan synthesis, including kre1∆, kre6∆, kre9∆, and big1∆, were sporulated to analyze the effect of ß‐1,6‐glucan defects on the spore wall. Except for kre6∆, these mutant spores were sensitive to treatment with ether, suggesting that the mutations perturb the integrity of the spore wall. Morphologically, the mutant spores were indistinguishable from wild‐type spores. They lacked significant sporulation defects partly because the chitos...
Source: Yeast - July 1, 2017 Category: Molecular Biology Authors: Hua ‐Ping Pan, Ning Wang, Hiroyuki Tachikawa, Hideki Nakanishi, Xiao‐Dong Gao Tags: Research Article Source Type: research
Multi ‐gene phylogenetic analysis reveals that shochu‐fermenting Saccharomyces cerevisiae strains form a distinct sub‐clade of the Japanese sake cluster
In this study, we performed the first (to our knowledge) phylogenetic classification of shochu strains based on nucleotide gene sequences. We performed phylogenetic classification of 21 putative indigenous shochu yeast strains isolated from 11 distilleries. All of these strains were shown or confirmed to be Saccharomyces cerevisiae, sharing species identification with 34 known S. cerevisiae strains (including commonly used shochu, sake, ale, whisky, bakery, bioethanol, and laboratory yeast strains and clinical isolate) that were tested in parallel. Our analysis used five genes that reflect genome‐level phylogeny for the ...
Source: Yeast - July 1, 2017 Category: Molecular Biology Authors: Taiki Futagami, Chihiro Kadooka, Yoshinori Ando, Kayu Okutsu, Yumiko Yoshizaki, Shinji Setoguchi, Kazunori Takamine, Mikihiko Kawai, Hisanori Tamaki Tags: Research Article Source Type: research
Cost ‐effective and rapid lysis of Saccharomyces cerevisiae cells for quantitative western blot analysis of proteins, including phosphorylated eIF2α
Abstract
The common method for liberating proteins from Saccharomyces cerevisiae cells involves mechanical cell disruption using glass beads and buffer containing inhibitors (protease, phosphatase and/or kinase inhibitors), followed by centrifugation to remove cell debris. This procedure requires the use of costly inhibitors and is laborious, in particular when many samples need to be processed. Also, enzymatic reactions can still occur during harvesting and cell breakage. As a result low‐abundance and labile proteins may be degraded, and enzymes such as kinases and phosphatases may still modify proteins during and after...
Source: Yeast - June 30, 2017 Category: Molecular Biology Authors: Su Jung Lee, Rashmi Ramesh, Valerie Boor, Jan M. Gebler, Richard C. Silva, Evelyn Sattlegger Tags: Research Article Source Type: research
The frenemies within: viruses, retrotransposons and plasmids that naturally infect Saccharomyces yeasts
This article has been written to briefly introduce viruses, retrotransposons and plasmids that infect Saccharomyces yeasts, emphasize some important cellular proteins and machineries with which they interact, and suggest the evolutionary consequences of these interactions. Copyright © 2017 John Wiley & Sons, Ltd.
Saccharomyces yeasts harbor a variety of intracellular nucleic acid elements that can impact the physiology and evolutionary fitness of their host. The lifecycles of these viruses, retrotransposons and plasmids provide a unique model to study the hijack and antagonism of eukaryotic cellular machineries, and ...
Source: Yeast - June 7, 2017 Category: Molecular Biology Authors: Paul A. Rowley Tags: Budding Topic Source Type: research
Issue Information ‐ Notes for Contribs p1
No abstract is available for this article. (Source: Yeast)
Source: Yeast - June 5, 2017 Category: Molecular Biology Tags: Issue Information ‐ Notes for Contribs p1 Source Type: research
Issue Information
No abstract is available for this article. (Source: Yeast)
Source: Yeast - June 5, 2017 Category: Molecular Biology Tags: Issue Information Source Type: research
Hybridization and emergence of virulence in opportunistic human yeast pathogens
Abstract
Hybridization between different species can result in the emergence of new lineages and adaptive phenotypes. Occasionally, hybridization in fungal organisms can drive the appearance of opportunistic lifestyles or shifts to new hosts, resulting in the emergence of novel pathogens. In recent years, an increasing number of studies have documented the existence of hybrids in diverse yeast clades, including some comprising human pathogens. Comparative and population genomics studies performed on these clades are enabling us to understand what roles hybridization may play in the evolution and emergence of a virulence po...
Source: Yeast - June 1, 2017 Category: Molecular Biology Authors: Ver ónica Mixão, Toni Gabaldón Tags: Research Article Source Type: research
Opi1p translocation to the nucleus is regulated by hydrogen peroxide in Saccharomyces cerevisiae
Abstract
During exposure of yeast cells to low levels of hydrogen peroxide (H2O2), expression of several genes is regulated for cells to adapt to the surrounding oxidative environment. Such adaptation involves modifying plasma membrane lipid composition, reorganization of ergosterol‐rich microdomains, and altered gene expression of proteins involved in lipid and vesicle traffic, to decrease permeability to exogenous H2O2. Opi1p is a transcriptional repressor that is inactive when present at the nuclear membrane/endoplasmic reticulum, but represses transcription of inositol upstream activating sequence (UASINO)‐containi...
Source: Yeast - June 1, 2017 Category: Molecular Biology Authors: Carolina Camelo, Filipe Vilas ‐Boas, Andreia Pereira Cepeda, Carla Real, Joana Barros‐Martins, Francisco Pinto, Helena Soares, H. Susana Marinho, Luisa Cyrne Tags: Research Article Source Type: research
Issue Information ‐ Notes for Contribs p1
No abstract is available for this article. (Source: Yeast)
Source: Yeast - May 4, 2017 Category: Molecular Biology Tags: Issue Information ‐ Notes for Contribs p1 Source Type: research
Issue Information
No abstract is available for this article. (Source: Yeast)
Source: Yeast - May 4, 2017 Category: Molecular Biology Tags: Issue Information Source Type: research
Cost ‐effective and rapid lysis of Saccharomyces cerevisiae cells, for quantitative western blot analysis of proteins, including phosphorylated eIF2α.
Abstract
The common method for liberating proteins from Saccharomyces cerevisiae cells involves mechanical cell disruption using glass beads and buffer containing inhibitors (protease, phosphatase, and/or kinase inhibitors), followed by centrifugation to remove cell debris. This procedure requires the use of costly inhibitors, and is laborious, in particular when many samples need to be processed. Also, enzymatic reactions can still occur during harvesting and cell breakage. As a result low abundant and labile proteins may be degraded, and enzymes such as kinases and phosphatases may still modify proteins during and after ...
Source: Yeast - May 1, 2017 Category: Molecular Biology Authors: Su Jung Lee, Rashmi Ramesh, Valerie Boor, Jan M Gebler, Richard C Silva, Evelyn Sattlegger Tags: Research Article Source Type: research
