Sterol synthesis and cell size distribution under oscillatory growth conditions in Saccharomyces cerevisiae scale ‐down cultivations
Summary
Physiologic responses of yeast to oscillatory environments as they appear in the liquid phase in large‐scale bioreactors were studied in the past. So far, however, the impact on the sterol household and intracellular regulation remains to be investigated. Since oxygen is a co‐factor in several reaction steps within the sterol metabolism, changes in oxygen availability, as it occurs in aerated bioreactors of production scales, might have an influence on the regulation and incorporation of free sterols into the cells’ lipid layer. Therefore, sterol and fatty acid synthesis in a two‐ and three‐compartment sc...
Source: Yeast - September 1, 2017 Category: Molecular Biology Authors: Anna ‐Maria Marbà‐Ardébol, Anika Bockisch, Peter Neubauer, Stefan Junne Tags: RESEARCH ARTICLE Source Type: research
Identification of a novel interspecific hybrid yeast from a metagenomic spontaneously inoculated beer sample using Hi ‐C
Abstract
Interspecific hybridization is a common mechanism enabling genetic diversification and adaptation; however, the detection of hybrid species has been quite difficult. The identification of microbial hybrids is made even more complicated, as most environmental microbes are resistant to culturing and must be studied in their native mixed communities. We have previously adapted the chromosome conformation capture method Hi‐C to the assembly of genomes from mixed populations. Here, we show the method's application in assembling genomes directly from an uncultured, mixed population from a spontaneously inoculated beer...
Source: Yeast - September 1, 2017 Category: Molecular Biology Authors: Caiti Smukowski Heil, Joshua N. Burton, Ivan Liachko, Anne Friedrich, Noah A. Hanson, Cody L. Morris, Joseph Schacherer, Jay Shendure, James H. Thomas, Maitreya J. Dunham Tags: Special Issue ‐ Yeast interspecies hybrids Source Type: research
The yeasts of the genus Spathaspora: potential candidates for second ‐generation biofuel production
Abstract
Yeasts of the Spathaspora clade have the ability to convert d‐xylose to ethanol and/or xylitol. This is an important trait, as these yeasts may be used to produce bioethanol from lignocellulosic biomass, or as a source of new d‐xylose metabolism genes for recombinant industrial strains of Saccharomyces cerevisiae. The core group of the genus Spathaspora has 22 species, both formally described and not yet described. Other species, such as Sp. allomyrinae, Candida alai, C. insectamans, C. lyxosophila, C. sake, Sp. boniae and C. subhashii are weakly associated with this clade, based on LSU rRNA gene D1/D2 sequenc...
Source: Yeast - September 1, 2017 Category: Molecular Biology Authors: Raquel M. Cadete, Carlos A. Rosa Tags: Yeast Primer Source Type: research
CRISPR/Cpf1 enables fast and simple genome editing of Saccharomyces cerevisiae
Abstract
Cpf1 represents a novel single RNA‐guided CRISPR/Cas endonuclease system suitable for genome editing with distinct features compared to Cas9. We demonstrate the functionality of three Cpf1 orthologues ‐ Acidaminococcus spp. BV3L6 (AsCpf1), Lachnospiraceae bacterium ND2006 (LbCpf1), Francisella novicida U112 (FnCpf1) ‐ for genome editing of Saccharomyces cerevisiae. These Cpf1‐based systems enable fast and reliable introduction of donor DNA on the genome using a two plasmid‐based editing approach together with linear donor DNA. LbCpf1 and FnCpf1 displayed editing efficiencies comparable to that of the CRI...
Source: Yeast - September 1, 2017 Category: Molecular Biology Authors: Ren é Verwaal, Nathalie Buiting‐Wiessenhaan, Sacha Dalhuijsen, Johannes A. Roubos Tags: Research Article Source Type: research
Division of labour in the yeast: Saccharomyces cerevisiae
Abstract
Division of labour between different specialized cell types is a central part of how we describe complexity in multicellular organisms. However, it is increasingly being recognized that division of labour also plays an important role in the lives of predominantly unicellular organisms. Saccharomyces cerevisiae displays several phenotypes that could be considered a division of labour, including quiescence, apoptosis and biofilm formation, but they have not been explicitly treated as such. We discuss each of these examples, using a definition of division of labour that involves phenotypic variation between cells wit...
Source: Yeast - August 25, 2017 Category: Molecular Biology Authors: Dominika M. Wloch ‐Salamon, Roberta M. Fisher, Birgitte Regenberg Tags: Budding Topic Source Type: research
Issue Information ‐ Notes for Contribs
No abstract is available for this article. (Source: Yeast)
Source: Yeast - August 3, 2017 Category: Molecular Biology Tags: Issue Information ‐ Notes for Contribs Source Type: research
Issue Information
No abstract is available for this article. (Source: Yeast)
Source: Yeast - August 3, 2017 Category: Molecular Biology Tags: Issue Information Source Type: research
From transcriptional complexity to cellular phenotypes: lessons from yeast.
Abstract
Pervasive transcription has been reported in many eukaryotic organisms, revealing a highly interleaved transcriptome organization that involves thousands of coding and non‐coding RNAs. However, to date, the biological impact of transcriptome complexity is still poorly understood. Here I will review how subtle variations of the transcriptome can lead to divergent cellular phenotypes by fine‐tuning both its coding potential and regulation. I will discuss strategies that can be used to link molecular variations with divergent biological outcomes. Finally, I will explore the implication of transcriptional complexi...
Source: Yeast - August 1, 2017 Category: Molecular Biology Authors: Vicent Pelechano Tags: Budding Topic Source Type: research
Transcriptional regulation of the protein kinase A subunits in Saccharomyces cerevisiae during fermentative growth
Abstract
Yeast cells can adapt their growth in response to the nutritional environment. Glucose is the favorite carbon source of Saccharomyces cerevisiae that prefers a fermentative metabolism despite the presence of oxygen. When glucose is consumed, the cell switches to the aerobic metabolism of ethanol, during the so‐called diauxic shift. The difference between fermentative and aerobic growth is in part mediated by a regulatory mechanism called glucose repression. During glucose derepression a profound gene transcriptional reprogramming occurs and genes involved in the utilization of alternative carbon sources are expr...
Source: Yeast - August 1, 2017 Category: Molecular Biology Authors: Fiorella Galello, Constanza Pautasso, Sol Reca, Luciana Ca ñonero, Paula Portela, Silvia Moreno, Silvia Rossi Tags: Research Article Source Type: research
Targeted gene replacement at the URA3 locus of the basidiomycetous yeast Pseudozyma antarctica and its transformation using lithium acetate treatment
In this study, we developed a method for targeted gene replacement in P. antarctica. In addition, transformation conditions were optimized using lithium acetate, single‐stranded carrier DNA, and polyethylene glycol (lithium acetate treatment) generally used for ascomycetous yeast transformation.
In the rice‐derived P. antarctica strain GB‐4(0), PaURA3, a homolog of the Saccharomyces cerevisiae orotidine‐5’‐phosphate decarboxylase gene (URA3), was selected as the target locus. A disruption cassette was constructed by linking the nouseothricine resistance gene (natMX4) to homologous DNA fragments of PaURA3, then ...
Source: Yeast - August 1, 2017 Category: Molecular Biology Authors: Tohru Yarimizu, Hitoshi Shimoi, Yuka Sameshima ‐Yamashita, Tomotake Morita, Hideaki Koike, Takashi Watanabe, Hiroko Kitamoto Tags: Research Article Source Type: research
The hybrid genomes of Saccharomyces pastorianus ‐a current perspective
Abstract
Saccharomyces pastorianus is a recently evolved interspecies hybrid of Saccharomyces cerevisiae and Saccharomyces eubayanus used in the production of lager‐type beers and has a long‐standing history with the brewing industry. At least two distinct types of lager yeasts (Groups I and II) have been identified based on chromosome content and structure. One important feature of the genomes of lager yeasts is the presence of a set of hybrid chromosomes that emerged as a result of homeologous recombination events between the parental chromosomes. The unique genetic composition of the hybrid genomes of S. pastorianus...
Source: Yeast - August 1, 2017 Category: Molecular Biology Authors: Chandre Monerawela, Ursula Bond Tags: Special Issue ‐ Yeast interspecies hybrids Source Type: research
Many interspecific chromosomal introgressions are highly prevalent in Holarctic Saccharomyces uvarum strains found in human ‐related fermentations
Abstract
In the last two decades, the massive genome sequencing of strains belonging to the Saccharomyces genus revealed the complex reticulated evolution of this group. Among the various evolutionary mechanisms described, the introgression of large chromosomal regions resulting from interspecific hybridization has recently shed light on the Saccharomyces uvarum species. In this work we provide the de novo assembled genomes of four S. uvarum strains presenting more than 712 kb of introgressed loci inherited from both S. eubayanus and S. kudriavzevii species. In order to study the prevalence of such introgressions in a larg...
Source: Yeast - August 1, 2017 Category: Molecular Biology Authors: Albertin Warren, Chernova Maria, Durrens Pascal, Guichou Erwan, Sherman David, Masneuf ‐Pomarede Isabelle, Philippe Marullo Tags: Special Issue ‐ Yeast interspecies hybrids Source Type: research
S. cerevisiae Gle2/Rae1 is involved in septin organization, essential for cell cycle progression
Abstract
Gle2/Rae1 is highly conserved from yeast to humans and described as an mRNA export factor. Additionally, it is implicated in the anaphase‐promoting complex (APC)‐mediated cell cycle regulation in higher eukaryotes. Here we identified an involvement for S. cerevisiae Gle2 in septin organization, which is crucial for cell cycle progression and cell division. Gle2 genetically and physically interacts with components of the septin‐ring. Importantly, deletion of GLE2 leads to elongated buds, severe defects in septin‐assembly and their cellular mislocalization. Septin‐ring formation is triggered by the septin...
Source: Yeast - August 1, 2017 Category: Molecular Biology Authors: Gesa Zander, Wilfried Kramer, Anika Seel, Heike Krebber Tags: Research Article Source Type: research
Identification by phenotypic and genetic approaches of an indigenous Saccharomyces cerevisiae wine strain with high desiccation tolerance
This study highlights the exploitation of natural yeast biodiversity as a source of hidden technological features and as an alternative approach to strain improvement by genetic modifications. (Source: Yeast)
Source: Yeast - July 21, 2017 Category: Molecular Biology Authors: Marianna Zambuto, Rossana Romaniello, Nicoletta Guaragnella, Patrizia Romano, Sonia Votta, Angela Capece Tags: Research Article Source Type: research
Issue Information ‐ Notes for Contribs
No abstract is available for this article. (Source: Yeast)
Source: Yeast - July 7, 2017 Category: Molecular Biology Tags: Issue Information ‐ Notes for Contribs Source Type: research
