Yeast culture collections in the twenty‐first century: New opportunities and challenges
This article is protected by copyright. All rights reserved. (Source: Yeast)
Source: Yeast - April 30, 2016 Category: Molecular Biology Authors: Kyria L. Boundy‐Mills, Ewald Glantschnig, Ian N. Roberts, Andrey Yurkov, Serge Casaregola, Heide‐Marie Daniel, Marizeth Groenewald, Benedetta Turchetti Tags: Research Article Source Type: research
Yeast Biota of Naturally Fermented Black Olives in Different Brines Made from cv. Gemlik Grown in Various Districts of Cukurova Region in Turkey
This article is protected by copyright. All rights reserved. (Source: Yeast)
Source: Yeast - April 30, 2016 Category: Molecular Biology Authors: Sezgi Leventdurur, Selvihan Sert‐Aydın, C. Pelin Boyaci‐Gunduz, Bilal Agirman, Akram Ben Ghorbal, Nicola Francesca, Alessandra Martorana, Hüseyin Erten Tags: Research Article Source Type: research
Role of Aif1 in regulation of cell death under environmental stress in Candida albicans
In this study, we find that the C. albicans AIF protein Aif1, similar to its homologs in other organisms, is localized at the mitochondria and translocated into the nucleus under apoptosis‐inducing conditions. Moreover, deletion of AIF1 causes attenuated apoptosis in this pathogen under apoptosis‐inducing conditions, such as the treatment of 2 mM H2O2, 10 mM acetic acid or 0.08 mg/L caspofungin, and its overexpression enhances this process. Interestingly, under the treatment of high levels of these agents lead to reversed sensitivity of aif1Δ/Δ and the overexpression strain AIF1ov. In addition, the virulence of...
Source: Yeast - April 27, 2016 Category: Molecular Biology Authors: Feiyang Ma, Yueqi Zhang, Yuzhou Wang, Yajuan Wan, Yunheng Miao, Tianyu Ma, Qilin Yu, Mingchun Li Tags: Research Article Source Type: research
Maltase protein of Ogataea (Hansenula) polymorpha is a counterpart to the resurrected ancestor protein ancMALS of yeast maltases and isomaltases
Abstract
Saccharomyces cerevisiae maltases use maltose, maltulose, turanose and maltotriose as substrates, isomaltases use isomaltose, α‐methylglucoside and palatinose and both use sucrose. These enzymes are hypothesized to have evolved from a promiscuous α‐glucosidase ancMALS through duplication and mutation of the genes. We studied substrate specificity of the maltase protein MAL1 from an earlier diverged yeast, Ogataea polymorpha (Op), in the light of this hypothesis. MAL1 has extended substrate specificity and its properties are strikingly similar to those of resurrected ancMALS. Moreover, amino acids considered ...
Source: Yeast - April 20, 2016 Category: Molecular Biology Authors: Katrin Viigand, Triinu Visnapuu, Karin Mardo, Anneli Aasamets, Tiina Alamäe Tags: ISSY32 Special Issue Source Type: research
Lipid production from hemicellulose with Lipomyces starkeyi in a pH regulated fed ‐batch cultivation
This study investigated lipid production from the hemicellulosic fraction of birch wood by the oleaginous yeast Lipomyces starkeyi. Birch wood chips were thermochemically pretreated by hot water extraction, and the liquid phase, containing 45.1 g/l xylose as the major sugar, 13.1 g/l acetic acid and 4.7 g/l furfural, was used for cultivations of L. starkeyi CBS1807. The hydrolysate strongly inhibited yeast growth; the strain could only grow in medium containing 30% hydrolysate at pH 6. At pH 5, growth stopped already upon the addition of about 10% hydrolysate. In fed‐batch cultures fed with hydrolysate or a model xylose...
Source: Yeast - April 18, 2016 Category: Molecular Biology Authors: Jule Brandenburg, Johanna Blomqvist, Jana Pickova, Nemailla Bonturi, Mats Sandgren, Volkmar Passoth Tags: ISSY32 Special Issue Source Type: research
Lipid production from hemicellulose with Lipomyces starkeyi in a pH regulated fed‐batch cultivation
This study investigated lipid production from the hemicellulosic fraction of birch wood by the oleaginous yeast Lipomyces starkeyi. Birch wood chips were thermochemically pretreated by hot water extraction, and the liquid phase, containing 45.1 g/l xylose as the major sugar, 13.1 g/l acetic acid and 4.7 g/l furfural, was used for cultivations of L. starkeyi CBS1807. The hydrolysate strongly inhibited yeast growth; the strain could only grow in medium containing 30% hydrolysate at pH 6. At pH 5, growth stopped already upon the addition of about 10% hydrolysate. In fed‐batch cultures fed with hydrolysate or a model xylose...
Source: Yeast - April 18, 2016 Category: Molecular Biology Authors: Jule Brandenburg, Johanna Blomqvist, Jana Pickova, Nemailla Bonturi, Mats Sandgren, Volkmar Passoth Tags: ISSY32 Special Issue Source Type: research
Partial purification of histone H3 proteolytic activity from the budding yeast Saccharomyces cerevisiae
Abstract
The proteolytic clipping of histone tails has recently emerged as a novel form of irreversible post‐translational modification (PTM) of histones. Histone clipping has been implicated as a regulatory process leading to the permanent removal of PTMs from histone proteins. However, there is scarcity of literature that describes the identification and characterization of histone‐specific proteases. Here, we employed various biochemical methods to report histone H3‐specific proteolytic activity from budding yeast. Our results demonstrate that H3 proteolytic activity was associated with sepharose bead matrices and...
Source: Yeast - April 6, 2016 Category: Molecular Biology Authors: Gajendra Kumar Azad, Raghuvir Singh Tomar Tags: Research Article Source Type: research
Proline accumulation protects Saccharomyces cerevisiae cells in stationary phase from ethanol stress by reducing reactive oxygen species levels
In this study, we examined the role of intracellular proline during ethanol treatment in S. cerevisiae strains that accumulate different concentrations of proline. When cultured in YPD medium, there was a significant accumulation of proline in the put1 mutant strain, which is deficient in proline oxidase, in the stationary phase. Expression of the mutant PRO1 gene, which encodes the γ‐glutamyl kinase variant (Asp154Asn or Ile150Thr) with desensitization to feedback inhibition by proline in the put1 mutant strain, showed a prominent increase in proline content as compared with that of the wild‐type strain. The oxidatio...
Source: Yeast - April 4, 2016 Category: Molecular Biology Authors: Hiroshi Takagi, Junpei Taguchi, Tomohiro Kaino Tags: ISSY32 Special Issue Source Type: research
Issue Information ‐ Notes for Contribs p1
No abstract is available for this article. (Source: Yeast)
Source: Yeast - April 3, 2016 Category: Molecular Biology Tags: Issue Information ‐ Notes for Contribs p1 Source Type: research
Issue Information ‐ Info Page
No abstract is available for this article. (Source: Yeast)
Source: Yeast - April 3, 2016 Category: Molecular Biology Tags: Issue Information ‐ Info Page Source Type: research
Issue Information ‐ TOC
No abstract is available for this article. (Source: Yeast)
Source: Yeast - April 3, 2016 Category: Molecular Biology Tags: Issue Information ‐ TOC Source Type: research
Yeast and mold dynamics in Caciofiore della Sibilla cheese coagulated with an aqueous extract of Carlina acanthifolia All.
Abstract
Caciofiore della Sibilla is a specialty ewe's milk cheese traditionally manufactured in a foothill area of the Marche region (Central Italy) with a crude extract of fresh young leaves of Carlina acanthifolia All. subsp. acanthifolia as a coagulating agent. The fungal dynamics and diversity of this specialty cheese were investigated throughout the manufacturing and 20‐day ripening process, using a combined PCR‐DGGE approach. The fungal biota of a control ewe's milk cheese manufactured with the same batch of milk coagulated with a commercial animal rennet was also monitored by PCR‐DGGE, in order to investigate...
Source: Yeast - March 31, 2016 Category: Molecular Biology Authors: Federica Cardinali, Manuela Taccari, Vesna Milanović, Andrea Osimani, Serena Polverigiani, Cristiana Garofalo, Roberta Foligni, Massimo Mozzon, Silvia Zitti, Nadia Raffaelli, Francesca Clementi, Lucia Aquilanti Tags: ISSY32 Special Issue Source Type: research
Influence of Lachancea thermotolerans on Cv. Emir Wine Fermentation
Abstract
The present work describes the behaviour of Lachancea thermotolerans and Saccharomyces cerevisiae in pure, co‐cultured and sequential fermentations in cv. Emir grape must. Faster fermentation rates were observed in the wine made with pure culture of S. cerevisiae and the wine produced with simultaneously inoculated cultures of L. thermotolerans and S. cerevisiae. Both L. thermotolerans and S. cerevisiae gave high population numbers. L. thermotolerans usage in mixed and sequential cultures led to an increase in final total acidity content in the wines, varying in the range of 5.40‐6.28 g/l as tartaric acid co...
Source: Yeast - March 31, 2016 Category: Molecular Biology Authors: Eren Kemal Balikci, Hasan Tanguler, Neil P. Jolly, Huseyin Erten Tags: ISSY32 Special Issue Source Type: research
A stable phylogeny of the large‐spored Metschnikowia clade
This article is protected by copyright. All rights reserved. (Source: Yeast)
Source: Yeast - March 27, 2016 Category: Molecular Biology Authors: Marc‐André Lachance, Emilia Hurtado, Tom Hsiang Tags: Research Article Source Type: research
Anhydrobiosis in yeast: cell wall mannoproteins are important for yeast Saccharomyces cerevisiae resistance to dehydration
This study was directed to understanding how changes in cell wall structure may influence the resistance of yeasts to dehydration‐rehydration. Mutants lacking various cell wall mannoproteins were tested to address this issue. It was revealed that mutants lacking proteins belonging to two structurally and functionally unrelated groups (proteins non‐covalently attached to cell wall and Pir proteins) possessed significantly lower cell resistance to dehydration‐rehydration than the mother wild‐type strain. At the same time, the absence of the GPI‐anchored cell wall protein Ccw12 unexpectedly resulted in an increase o...
Source: Yeast - March 27, 2016 Category: Molecular Biology Authors: Diana Borovikova, Renata Teparić, Vladimir Mrša, Alexander Rapoport Tags: ISSY32 Special Issue Source Type: research
