Emodin, a natural inhibitor of protein kinase CK2, suppresses growth, hyphal development, and biofilm formation of Candida albicans
Abstract
Emodin (1,3,8‐trihydroxy‐6‐methyl‐anthraquinone) is a natural secondary plant product, originally isolated from the rhizomes of Rheum palmatum. Many reports show its diuretic, vasorelaxant, anti‐bacterial, anti‐viral, anti‐ulcerogenic, immunosuppressive, hepatoprotective, anti‐inflammatory, and anti‐cancer potential. Emodin is a pleiotropic molecule capable of interacting with several major molecular targets, e.g. NF‐κB, AKT/mTOR, and STAT3. The compound can also act as an inhibitor of some protein kinases, with special affinity to protein kinase CK2. The aim of the presented report was to eva...
Source: Yeast - January 31, 2017 Category: Molecular Biology Authors: Monika Janeczko, Maciej Mas łyk, Konrad Kubiński, Hieronim Golczyk Tags: Research Article Source Type: research
Saccharomyces cerevisiae Atf1p is an alcohol acetyltransferase and a thioesterase in vitro
Abstract
The alcohol‐O‐acyltransferases are bisubstrate enzymes that catalyse the transfer of acyl chains from an acyl‐CoA donor to an acceptor alcohol. In the industrial yeast Saccharomyces cerevisiae this reaction produces acyl esters that are an important influence on the flavor of fermented beverages and foods. There is also a growing interest in using acyltransferases to produce bulk quantities of acyl esters in engineered microbial cell factories. However, the structure and function of the alcohol‐O‐acyltransferases remain only partly understood. Here, we recombinantly express, purify and characterise Atf1p...
Source: Yeast - January 31, 2017 Category: Molecular Biology Authors: Bethany Nancolas, Ian D. Bull, Richard Stenner, Virginie Dufour, Paul Curnow Tags: Research Article Source Type: research
Analysis of Paracoccidioides lutzii mitochondria: a proteomic approach
Abstract
The genus Paracoccidioides is composed of thermal dimorphic fungi, causative agents of paracoccidioidomycosis, one of the most frequent systemic mycoses in Latin America. Mitochondria have sophisticated machinery for ATP production, which involves metabolic pathways such as citric acid and glyoxylate cycles, electron transport chain and oxidative phosphorylation. In addition, this organelle performs a variety of functions in the cell, working as an exceptional metabolic signalling centre that contributes to cellular stress responses, as autophagy and apoptosis in eukaryotic organisms. The aim of this work was to p...
Source: Yeast - January 25, 2017 Category: Molecular Biology Authors: L. Casaletti, P. S. Lima, L. N. Oliveira, C. L. Borges, S. N. B áo, A. M. Bailão, C. M. A. Soares Tags: Research Article Source Type: research
Issue Information ‐ Notes for Contribs p1
No abstract is available for this article. (Source: Yeast)
Source: Yeast - January 12, 2017 Category: Molecular Biology Tags: Issue Information ‐ Notes for Contribs p1 Source Type: research
Use of a fluoride channel as a new selection marker for fission yeast plasmids and application to fast genome editing with CRISPR/Cas9
(Source: Yeast)
Source: Yeast - January 12, 2017 Category: Molecular Biology Tags: Erratum Source Type: research
Issue Information
No abstract is available for this article. (Source: Yeast)
Source: Yeast - January 12, 2017 Category: Molecular Biology Tags: Issue Information Source Type: research
Issue Information ‐ Notes for Contribs p1
No abstract is available for this article. (Source: Yeast)
Source: Yeast - December 4, 2016 Category: Molecular Biology Tags: Issue Information ‐ Notes for Contribs p1 Source Type: research
Issue Information ‐ Info Page
No abstract is available for this article. (Source: Yeast)
Source: Yeast - December 4, 2016 Category: Molecular Biology Tags: Issue Information ‐ Info Page Source Type: research
Issue Information ‐ TOC
No abstract is available for this article. (Source: Yeast)
Source: Yeast - December 4, 2016 Category: Molecular Biology Tags: Issue Information ‐ TOC Source Type: research
Saccharomyces cerevisiae shuttle vectors
Abstract
Yeast shuttle vectors are indispensable tools in yeast research. They enable cloning of defined DNA sequences in Escherichia coli and their direct transfer into Saccharomyces cerevisiae cells. There are three types of commonly used yeast shuttle vectors: centromeric plasmids, episomal plasmids and integrating plasmids. In this review, we discuss the different plasmid systems and their characteristic features. We focus on their segregational stability and copy number and indicate how to modify these properties. (Source: Yeast)
Source: Yeast - November 30, 2016 Category: Molecular Biology Authors: Robert Gn ügge, Fabian Rudolf Tags: Review Source Type: research
N ‐ICE plasmids for generating N‐terminal 3 × FLAG tagged genes that allow Inducible, Constitutive, or Endogenous expression in Saccharomyces cerevisiae
Abstract
PCR‐mediated homologous recombination is a powerful approach to introduce epitope tags into the chromosomal loci at the N‐terminus or the C‐terminus of targeted genes. Although strategies of C‐terminal epitope tagging of target genes at their loci are simple and widely used in yeast, C‐terminal epitope tagging is not practical for all proteins. For example, a C‐terminal tag may affect protein function or a protein may get cleaved or processed resulting in the loss of the epitope tag. Therefore, N‐terminal epitope tagging may be necessary to resolve these problems. In some cases, an epitope tagging st...
Source: Yeast - November 30, 2016 Category: Molecular Biology Authors: Yueping Zhang, Nina D. Serratore, Scott D. Briggs Tags: Research Article Source Type: research
Modulation of meiotic homologous recombination by DNA helicases
This article is protected by copyright. All rights reserved. (Source: Yeast)
Source: Yeast - November 30, 2016 Category: Molecular Biology Authors: Alexander Lorenz Tags: Budding Topic Source Type: research
The regulatable MAL32 promoter in Saccharomyces cerevisiae: characteristics and tools to facilitate its use
Abstract
Here we describe a set of tools to facilitate the use of maltose and the MAL32 promoter for regulated gene expression in yeast, alone or in combination with the GAL1 promoter. Using fluorescent protein reporters we find that under non‐inducing conditions the MAL32 promoter exhibits a low basal level of expression, similar to the GAL1 promoter, and that both promoters can be induced independently of each other using the respective sugars, maltose and galactose. While their repression upon glucose addition is immediate and complete, we found that the MAL32 and GAL1 promoters each exhibit distinct induction kinetic...
Source: Yeast - November 22, 2016 Category: Molecular Biology Authors: Matthias Meurer, Veronika Chevyreva, Bram Cerulus, Michael Knop Tags: Research Article Source Type: research
Issue Information ‐ Notes for Contribs p1
No abstract is available for this article. (Source: Yeast)
Source: Yeast - November 5, 2016 Category: Molecular Biology Tags: Issue Information ‐ Notes for Contribs p1 Source Type: research
Issue Information ‐ Info Page
No abstract is available for this article. (Source: Yeast)
Source: Yeast - November 5, 2016 Category: Molecular Biology Tags: Issue Information ‐ Info Page Source Type: research
