The spoilage yeast Zygosaccharomyces bailii: foe or friend?
Abstract
Zygosaccharomyces bailii is a non‐Saccharomyces budding yeast known as one of the most aggressive food spoilage microorganism, often isolated as contaminant during wine fermentation, as well as from many acidic, high sugar and canned foods. The spoilage ability relies on the yeast's unique feature to tolerate the most common preservatives such as sulfite, dimethyl dicarbonate, acetic acid, and sorbic acid. Therefore, many studies focused on the description of this peculiar tolerance with the aim of developing preventative measures against Z. bailii food spoilage. These studies demonstrated the involvement of div...
Source: Yeast - May 1, 2017 Category: Molecular Biology Authors: Nurzhan Kuanyshev, Giusy M. Adamo, Danilo Porro, Paola Branduardi Tags: Yeast Primer Source Type: research
Characterization the carotenoid productions and profiles of three Rhodosporidium toruloides mutants from Agrobacterium tumefaciens ‐mediated transformation.
Abstract
The red yeast Rhodosporidium toruloides is a known lipid producer capable of accumulating large amounts of triacylglycerols and carotenoids. However, it remains challenging to study its carotenoid production profiles due to limited biochemical information and inefficient genetic tools. Here we used an Agrobacterium tumefaciens‐mediated transformation (ATMT) to change its carotenoid production and profiles. We constructed R. toruloides NP11 mutant libraries with ATMT, selected 3 mutants with different colors, characterized their carotenoid products by high pressure liquid chromatography‐mass spectrometry (HPLC...
Source: Yeast - April 20, 2017 Category: Molecular Biology Authors: Xinping Lin, Ning Gao, Sasa Liu, Sufang Zhang, Shuang Song, Chaofan Ji, Xiuping Dong, Yichen Su, Zongbao Kent Zhao, Beiwei Zhu Tags: Research Article Source Type: research
Transforming sugars into fat – lipid biosynthesis using different sugars in Yarrowia lipolytica
Abstract
In an era of ever‐increasing energy demands, a promising technology is being developed: the use of oleaginous microorganisms such as Yarrowia lipolytica to convert waste materials into biofuels. Here, we constructed two Y. lipolytica strains that displayed both increased lipid accumulation and more efficient use of biomass‐derived sugars, including glucose, fructose, galactose and inulin. The first strain, Y. lipolytica YLZ150, was derived from the French wild‐type strain W29. It had inhibited triacylglycerol mobilization (∆tgl4) and β‐oxidation (∆pox1–6), and it overexpressed GPD1, DGA2, HXK1, the ...
Source: Yeast - April 20, 2017 Category: Molecular Biology Authors: Piotr Hapeta, Magdalena Rakicka, Remi Dulermo, Heber Gamboa ‐Meléndez, Anne‐Marie Cruz‐Le Coq, Jean‐Marc Nicaud, Zbigniew Lazar Tags: Research Article Source Type: research
Issue Information ‐ Notes for Contribs p1
No abstract is available for this article. (Source: Yeast)
Source: Yeast - April 6, 2017 Category: Molecular Biology Tags: Issue Information ‐ Notes for Contribs p1 Source Type: research
Issue Information
No abstract is available for this article. (Source: Yeast)
Source: Yeast - April 6, 2017 Category: Molecular Biology Tags: Issue Information Source Type: research
The Neutral N ‐linked Glycans of the Ustilaginomycete Yeast Sympodiomycopsis paphiopedili
In this study, the neutral N‐linked glycans of S. paphiopedili were prepared and structurally analyzed using high performance liquid chromatography (HPLC) and mass spectrometry (MS). Glycosidase digestion analyses were also performed to verify certain glycan linkages. HPLC and MS analyses revealed the presence of neutral N‐linked glycans ranging from Man3GlcNAc2‐PA to Man9GlcNAc2‐PA in length. The most abundant neutral N‐linked glycan structure in this species was found to be the Manα1‐2Manα1‐6(Manα1‐3)Manα1‐6(Manα1‐2Manα1‐2Manα1‐3)Manβ1‐4GlcNAcβ1‐4GlcNAc (M8A). Moreover, the second and...
Source: Yeast - April 6, 2017 Category: Molecular Biology Authors: Ronilo Jose D. Flores, Takao Ohashi, Hiroko Kawasaki, Kazuhito Fujiyama Tags: Research Article Source Type: research
A multiplex culture system for the long ‐term growth of fission yeast cells
Abstract
Maintenance of long‐term cultures of yeast cells is central to a broad range of investigations, from metabolic studies to laboratory evolution assays. However, repeated dilutions of batch cultures lead to variations in medium composition, with implications for cell physiology. In S. cerevisiae, powerful miniaturized chemostat setups, or ministat arrays, have been shown to allow for constant dilution of multiple independent cultures. Here we set out to adapt these arrays for continuous culture of a morphologically and physiologically distinct yeast, the fission yeast Schizosaccharomyces pombe, with the goal of ma...
Source: Yeast - April 1, 2017 Category: Molecular Biology Authors: Celine Callens, Nelson C. Coelho, Aaron W. Miller, Maria Rosa Domino Sananes, Maitreya J. Dunham, Matthieu Denoual, Damien Coudreuse Tags: Research Article Source Type: research
An Estradiol ‐Inducible Promoter Enables Fast, Graduated Control of Gene Expression in Fission Yeast
Abstract
The fission yeast Schizosaccharomyces pombe lacks a diverse toolkit of inducible promoters for experimental manipulation. Available inducible promoters suffer from slow induction kinetics, limited control of expression levels and/or a requirement for defined growth medium. In particular, no S. pombe inducible promoter systems exhibit a linear dose response, which would allow expression to be tuned to specific levels. We have adapted a fast, orthogonal promoter system with a large dynamic range and a linear dose response, based on β‐estradiol‐regulated function of the human estrogen receptor, for use in S. pom...
Source: Yeast - April 1, 2017 Category: Molecular Biology Authors: Makoto J. Ohira, David G. Hendrickson, R. Scott McIsaac, Nicholas Rhind Tags: Research Article Source Type: research
The frenemies within: Viruses, retrotransposons, and plasmids that naturally infect Saccharomyces yeasts.
Abstract
Viruses are a major focus of current research efforts because of their detrimental impact on humanity and their ubiquity within the environment. Bacteriophages have long been used to study host‐virus interactions within microbes, but it is often forgotten that the single‐celled eukaryote Saccharomyces cerevisiae and related species are infected with double‐stranded RNA viruses, single stranded RNA viruses, LTR‐retrotransposons and double‐stranded DNA plasmids. These intracellular nucleic acid elements have some similarities to higher eukaryotic viruses, i.e. yeast retrotransposons have an analogous lifec...
Source: Yeast - April 1, 2017 Category: Molecular Biology Authors: Paul A. Rowley Tags: Budding Topic Source Type: research
Issue Information ‐ Notes for Contribs p1
No abstract is available for this article. (Source: Yeast)
Source: Yeast - March 2, 2017 Category: Molecular Biology Tags: Issue Information ‐ Notes for Contribs p1 Source Type: research
Issue Information
No abstract is available for this article. (Source: Yeast)
Source: Yeast - March 2, 2017 Category: Molecular Biology Tags: Issue Information Source Type: research
Transforming sugars into fat —lipid biosynthesis using different sugars in Yarrowia lipolytica
Abstract
In an era of ever‐increasing energy demands, a promising technology is being developed: the use of oleaginous microorganisms such as Yarrowia lipolytica to convert waste materials into biofuels. Here, we constructed two Y. lipolytica strains that displayed both increased lipid accumulation and a more efficient use of biomass‐derived sugars, including glucose, fructose, galactose, and inulin. The first strain, Y. lipolytica YLZ150, was derived from the French wild‐type strain W29. It had inhibited triacylglycerol mobilization (∆tgl4) and β‐oxidation (∆pox1‐6), and it overexpressed GPD1, DGA2, HXK1, t...
Source: Yeast - February 28, 2017 Category: Molecular Biology Authors: Piotr Hapeta, Magdalena Rakicka, Remi Dulermo, Heber Gamboa ‐Meléndez, Anne‐Marie Crutz‐Le Coq, Jean‐Marc Nicaud, Zbigniew Lazar Tags: Research Article Source Type: research
Issue Information ‐ Notes for Contribs p1
No abstract is available for this article. (Source: Yeast)
Source: Yeast - February 2, 2017 Category: Molecular Biology Tags: Issue Information ‐ Notes for Contribs p1 Source Type: research
A Set of Isomeric Episomal Plasmids for Systematic Examination of Mitotic Stability in Saccharomyces cerevisiae
Abstract
Yeast episomal shuttle vectors (YEp type) are commonly used in fundamental research and biotechnology whenever elevated product levels are desired. Their instability, however, poses an impediment not only in industrial scale fermentation. In order to analyze instability which might be linked to plasmid structure, a series of YEp type plasmids identical in size has been assembled, differing only in the overall arrangement of the fragments used. Performance of the eight plasmid isoforms was studied with respect to mitotic stability. While transformation efficiency in two laboratory strains of Saccharomyces cerevisia...
Source: Yeast - January 31, 2017 Category: Molecular Biology Authors: Ruben Hohnholz, Kim Julia Pohlmann, Tilman Achstetter Tags: Research Article Source Type: research
