Characterization the carotenoid productions and profiles of three Rhodosporidium toruloides mutants from Agrobacterium tumefaciens ‐mediated transformation.

Abstract The red yeast Rhodosporidium toruloides is a known lipid producer capable of accumulating large amounts of triacylglycerols and carotenoids. However, it remains challenging to study its carotenoid production profiles due to limited biochemical information and inefficient genetic tools. Here we used an Agrobacterium tumefaciens‐mediated transformation (ATMT) to change its carotenoid production and profiles. We constructed R. toruloides NP11 mutant libraries with ATMT, selected 3 mutants with different colors, characterized their carotenoid products by high pressure liquid chromatography‐mass spectrometry (HPLC‐MS) analysis, and assured differences among those strains in terms of carotenoid production and its composition profiles. We then located T‐DNA insertion sites by using the genome walking technology and provided discussions in terms of the new phenotypes. This is the first of its kind to change the carotenoid production profiles in R. toruloides.
Source: Yeast - Category: Molecular Biology Authors: Tags: Research Article Source Type: research