The natural diversity and ecology of fission yeast
Yeast,Volume 35, Issue 3, Page 253-260, March 2018. (Source: Yeast)
Source: Yeast - October 30, 2017 Category: Molecular Biology Source Type: research
A combinatorial approach to synthetic transcription factor ‐promoter combinations for yeast strain engineering
Yeast,Volume 35, Issue 3, Page 273-280, March 2018. (Source: Yeast)
Source: Yeast - October 30, 2017 Category: Molecular Biology Source Type: research
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Yeast,Volume 35, Issue 3, Page 253-260, March 2018. (Source: Yeast)
Source: Yeast - October 30, 2017 Category: Molecular Biology Source Type: research
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Yeast,Volume 35, Issue 3, Page 273-280, March 2018. (Source: Yeast)
Source: Yeast - October 30, 2017 Category: Molecular Biology Source Type: research
A combinatorial approach to Synthetic Transcription Factor ‐Promoter combinations for yeast strain engineering
Abstract
Despite the need for inducible promoters in strain development efforts, the majority of engineering in Saccharomyces cerevisiae continues to rely on a few constitutively active or inducible promoters. Building on advances that use the modular nature of both transcription factors and promoter regions, we have built a library of hybrid promoters that are regulated by a synthetic transcription factor. The hybrid promoters consist of native S. cerevisiae promoters, in which the operator regions have been replaced with sequences that are recognized by the bacterial LexA DNA binding protein. Correspondingly, the synthet...
Source: Yeast - October 30, 2017 Category: Molecular Biology Authors: Zain Y. Dossani, Amanda Reider Apel, Heather S. Middleton, Nathan J. Hillson, Samuel Deutch, Jay D. Keasling, Aindrila Mukhopadhyay Tags: RESEARCH ARTICLE Source Type: research
On the catabolism of amino acids in the yeast Dekkera bruxellensis and the implications for industrial fermentation processes
Yeast,Volume 35, Issue 3, Page 299-309, March 2018. (Source: Yeast)
Source: Yeast - October 24, 2017 Category: Molecular Biology Source Type: research
The fission yeast MAPK Spc1 senses perturbations in Cdc25 and Wee1 activities and targets Rad24 to restore this balance
Yeast,Volume 35, Issue 3, Page 261-271, March 2018. (Source: Yeast)
Source: Yeast - October 24, 2017 Category: Molecular Biology Source Type: research
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Yeast,Volume 35, Issue 3, Page 299-309, March 2018. (Source: Yeast)
Source: Yeast - October 24, 2017 Category: Molecular Biology Source Type: research
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Yeast,Volume 35, Issue 3, Page 261-271, March 2018. (Source: Yeast)
Source: Yeast - October 24, 2017 Category: Molecular Biology Source Type: research
The fission yeast MAPK Spc1 senses perturbations in Cdc25 and Wee1 activities and targets Rad24 to restore this balance
Summary
Mitogen Activated Protein Kinases (MAPKs) play vital roles in multiple cellular processes and represent prominently pursued targets for development of therapeutic regimes. The MAPK Spc1 (p38 homolog) is known to be very important for both mitotic promotion and delay in Schizosaccharomyces pombe. However, the mechanism responsible for mitotic inhibition has remained elusive. Cdc25 (Cdc2 activator) and Wee1 (Cdc2 inhibtor) are important determinants of mitotic timing in all eukaryotes. Our results show that Spc1 can sense the perturbations in the balance of Cdc25 and Wee1 activities in S. pombe and that its function ...
Source: Yeast - October 24, 2017 Category: Molecular Biology Authors: Madhurima Paul, Agamani Ghosal, Sushobhana Bandyopadhyay, G. Prakadeeswari, Upasna Selvam, Neeraj Rai, Geetanjali Sundaram Tags: RESEARCH ARTICLE Source Type: research
Inhibition of yeast ‐to‐filamentous growth transitions in Candida albicans by a small molecule inducer of mammalian apoptosis
Yeast,Volume 35, Issue 3, Page 291-298, March 2018. (Source: Yeast)
Source: Yeast - October 19, 2017 Category: Molecular Biology Source Type: research
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Yeast,Volume 35, Issue 3, Page 291-298, March 2018. (Source: Yeast)
Source: Yeast - October 19, 2017 Category: Molecular Biology Source Type: research
Inhibition of Yeast ‐to‐Filamentous Growth Transitions in Candida albicans by a Small Molecule Inducer of Mammalian Apoptosis
In this study, we have screened additional inducers of mammalian apoptosis and identified BH3I‐1, as well as several structural derivatives of BH3I‐1, that act as specific inhibitors of morphogenesis under a variety of environmental conditions. Chemical epistasis experiments suggest that BH3I‐1 acts downstream of the hypha‐specific gene regulators Rfg1, Nrg1 and Ume6. (Source: Yeast)
Source: Yeast - October 19, 2017 Category: Molecular Biology Authors: Joy Goffena, Kurt A. Toenjes, David K. Butler Tags: RESEARCH ARTICLE Source Type: research
PER1, GUP1, and CWH43 of methylotrophic yeast Ogataea minuta are involved in cell wall integrity
SUMMARY
In eukaryotes, the glycosylphosphatidylinositol (GPI) modification of many glycoproteins on the cell surface is highly conserved. The lipid moieties of GPI‐anchored proteins undergo remodeling processes during their maturation. To date, the products of the PER1, GUP1, and CWH43 genes of the yeast Saccharomyces cerevisiae have been shown to be involved in the lipid remodeling. Here, we focus on the putative GPI remodeling pathway in the methylotrophic yeast Ogataea minuta. We found that the O. minuta homologs of PER1, GUP1, and CWH43 are functionally compatible with those of S. cerevisiae. Disruption of GUP1 or CW...
Source: Yeast - October 13, 2017 Category: Molecular Biology Authors: Xin ‐Xin Xu, Akiko Komatsuzaki, Yasunori Chiba, Xiao‐Dong Gao, Takehiko Yoko‐o Tags: RESEARCH ARTICLE Source Type: research
From transcriptional complexity to cellular phenotypes: Lessons from yeast
Abstract
Pervasive transcription has been reported in many eukaryotic organisms, revealing a highly interleaved transcriptome organization that involves thousands of coding and non‐coding RNAs. However, to date, the biological impact of transcriptome complexity is still poorly understood. Here I will review how subtle variations of the transcriptome can lead to divergent cellular phenotypes by fine‐tuning both its coding potential and regulation. I will discuss strategies that can be used to link molecular variations with divergent biological outcomes. Finally, I will explore the implication of transcriptional complexi...
Source: Yeast - October 11, 2017 Category: Molecular Biology Authors: Vicent Pelechano Tags: Budding Topic Source Type: research
