Issue Information ‐ TOC
No abstract is available for this article. (Source: Yeast)
Source: Yeast - November 5, 2016 Category: Molecular Biology Tags: Issue Information ‐ TOC Source Type: research
Analysis of Paracoccidioides lutzii, mitochondria: a proteomic approach
This article is protected by copyright. All rights reserved. (Source: Yeast)
Source: Yeast - October 31, 2016 Category: Molecular Biology Authors: L. Casaletti, P. S. Lima, L. N. Lima, C. L. Borges, S. N. B áo, A. M. Bailão, C. M. A. Bailão Tags: Research Article Source Type: research
Specific populations of the yeast Geotrichum candidum revealed by molecular typing
Abstract
Geotrichum candidum is an ubiquitous yeast, and an essential component in the production of many soft cheeses. We developed a Multi‐Locus Sequence Typing (MLST) scheme with five retained loci (NUP116, URA1, URA3, SAPT4, PLB3) which were sufficiently divergent to distinguish 40 Sequence Types (STs) among the 67 G. candidum strains tested. Phylogenetic analyses defined five main clades; one clade was restricted to environmental isolates, three other clades included distinct environmental isolates and dairy strains, while the fifth clade comprised 34 strains (13 STs), among which all but two were isolated from mi...
Source: Yeast - October 31, 2016 Category: Molecular Biology Authors: No émie Jacques, Sandrine Mallet, Fatima Laaghouiti, Colin R. Tinsley, Serge Casaregola Tags: Research Article Source Type: research
It's not all about us: Evolution and maintenance of Cryptococcus virulence requires selection outside the human host
Abstract
Cryptococcus is predominantly an AIDS‐related pathogen that causes significant morbidity and mortality in immunocompromised patients. Research studies have historically focused on understanding how the organism causes human disease through the use of in vivo and in vitro model systems to identify virulence factors. Cryptococcus is not an obligate pathogen, however, as human‐human transmission is either absent or rare. Selection in the environment must thus be invoked to shape the evolution of this taxa, and directly influences genotypic and trait diversity. Importantly, the evolution and maintenance of pathoge...
Source: Yeast - October 31, 2016 Category: Molecular Biology Authors: Aleeza C. Gerstein, Kirsten Nielsen Tags: Budding Topic Source Type: research
Arp2/3 complex and Mps3 are required for regulation of ribosome biosynthesis in the secretory stress response
In this study, we demonstrated that the Arp2/3 complex was required for reduction of ribosome protein gene expression in response to defective secretion by addition of tunicamycin. Two cmd1 mutants, cmd1‐228 and cmd1‐239 that cause mislocalization of calmodulin and defective mitotic spindle formation, respectively, fail to interact with Arc35, a component of the Arp2/3 complex. These mutants also caused defects in the reduction of ribosome protein gene expression induced by secretory blockade. A mutation in TUB4 (tub4‐1), whose product has an essential function in microtubule organization, showed a similar response. ...
Source: Yeast - October 31, 2016 Category: Molecular Biology Authors: Yukari Yabuki, Masako Katayama, Yushi Kodama, Akiko Sakamoto, Ayumi Yatsuhashi, Kouichi Funato, Keiko Mizuta Tags: Research Article Source Type: research
Cell surface hydrophobicity and colony morphology of Trichosporon asahii clinical isolates
Abstract
Trichosporon asahii is a pathogenic basidiomycetous yeast. Individual strains of T. asahii have different colony morphologies. However, it is not clear whether cell surface phenotypes differ among the colony morphologies. Here we characterized the cell surface hydrophobicity and analyzed the carbohydrate contents of the cell surface polysaccharides in T. asahii clinical isolates with various colony morphologies. Among the three distinctive colony morphologies obtained from one clinical isolate, the white‐type morphology exhibited higher hydrophobicity. The hydrophobicity of heat‐killed T. asahii cells was grea...
Source: Yeast - October 31, 2016 Category: Molecular Biology Authors: Tomoe Ichikawa, Chihiro Hirata, Mizuki Takei, Naoyuki Tagami, Hiromi Murasawa, Reiko Ikeda Tags: Research Article Source Type: research
Metschnikowia: half tetrads, a regicide and the fountain of youth
Abstract
The purpose of this review is to introduce Metschnikowia to the yeast researcher community and to convince readers that the genus is a worthwhile object of study in developmental biology, genetics, ecology and biotechnology. Metschnikowia sits at the foundation of modern immunology, having been instrumental in the discovery of animal phagocytosis. Some 81 species form a monophyletic group within the Metschnikowiaceae, which also include the smaller genus Clavispora and a few clades of Candida species. The family stands out by the habit of forming, by meiosis, only two ascospores, which in Metschnikowia are needle ...
Source: Yeast - October 16, 2016 Category: Molecular Biology Authors: Marc ‐André Lachance Tags: Yeast Primer Source Type: research
Use of ade1 and ade2 mutations for development of a versatile red/white colour assay of amyloid ‐induced oxidative stress in saccharomyces cerevisiae
Abstract
Mutations in adenine biosynthesis pathway genes ADE1 and ADE2 have been conventionally used to score for prion [PSI+] in yeast. If ade1‐14 mutant allele is present, which contains a premature stop codon, [psi−] yeast appear red on YPD medium owing to accumulation of a red intermediate compound in vacuoles. In [PSI+] yeast, partial inactivation of the translation termination factor, Sup35 protein, owing to its amyloid aggregation allows for read‐through of the ade1‐14 stop codon and the yeast appears white as the red intermediate pigment is not accumulated. The red colour development in ade1 and ade2 mutant...
Source: Yeast - October 12, 2016 Category: Molecular Biology Authors: Vidhya Bharathi, Amandeep Girdhar, Archana Prasad, Meenkshi Verma, Vibha Taneja, Basant K. Patel Tags: Research Article Source Type: research
Issue Information ‐ Notes for Contribs p1
No abstract is available for this article. (Source: Yeast)
Source: Yeast - October 4, 2016 Category: Molecular Biology Tags: Issue Information ‐ Notes for Contribs p1 Source Type: research
Issue Information ‐ Info Page
No abstract is available for this article. (Source: Yeast)
Source: Yeast - October 4, 2016 Category: Molecular Biology Tags: Issue Information ‐ Info Page Source Type: research
Issue Information ‐ TOC
No abstract is available for this article. (Source: Yeast)
Source: Yeast - October 4, 2016 Category: Molecular Biology Tags: Issue Information ‐ TOC Source Type: research
Yeasts found in vineyards and wineries
Abstract
Wine is a complex beverage, comprising thousands of metabolites that are produced through the action of a plethora of yeasts and bacteria during fermentation of grape must. These microbial communities originate in the vineyard and the winery and reflect the influence of several factors including grape variety, geographical location, climate, vineyard spraying, technological practices, processing stage and season (pre‐harvest, harvest, post‐harvest). Vineyard and winery microbial communities have the potential to participate during fermentation and influence wine flavour and aroma. Therefore, there is an enormo...
Source: Yeast - September 30, 2016 Category: Molecular Biology Authors: Cristian Varela, Anthony R Borneman Tags: Yeast Primer Source Type: research
Naumovozyma castellii: an alternative model for budding yeast molecular biology
Abstract
Naumovozyma castellii (Saccharomyces castellii) is a member of the budding yeast family Saccharomycetaceae. It has been extensively used as a model organism for telomere biology research and has gained an increasing interest as a budding yeast model for functional analyses owing to its amenability to genetic modifications. Due to the suitable phylogenetic distance to S. cerevisiae the whole genome sequence of N. castellii has provided unique data for comparative genomic studies, and it played a key role in the establishment of the timing of the whole genome duplication and the evolutionary events that took place i...
Source: Yeast - September 30, 2016 Category: Molecular Biology Authors: Ahu Karademir Andersson, Marita Cohn Tags: Yeast Primer Source Type: research
Spatial organization of the Schizosaccharomyces pombe genome within the nucleus
Abstract
The fission yeast Schizosaccharomyces pombe is a useful experimental system for studying the organization of chromosomes within the cell nucleus. S. pombe has a small genome that is organized into three chromosomes. The small size of the genome and the small number of chromosomes are advantageous for cytological and genome‐wide studies of chromosomes; however, the small size of the nucleus impedes microscopic observations owing to limits in spatial resolution during imaging. Recent advances in microscopy, such as super‐resolution microscopy, have greatly expanded the use of S. pombe as a model organism in a wi...
Source: Yeast - September 30, 2016 Category: Molecular Biology Authors: Atsushi Matsuda, Haruhiko Asakawa, Tokuko Haraguchi, Yasushi Hiraoka Tags: Budding Topic Source Type: research
Characterization of phosphatidic acid phosphatase activity in the oleaginous yeast Yarrowia lipolytica and its role in lipid biosynthesis
In this study, PAP activity in Y. lipolytica was characterized in detail and its role in lipid biosynthesis was addressed. PAP activity increased 2.5 fold with the addition of Mg2+ (1 mM) in the assay mixture, which means that most of the PAP activity was due to Mg2+‐dependent PAP enzymes (e.g. Pah1, App1). In contrast, N‐ethylmaleimide (NEM) potently inhibited PAP activity indicating the presence of NEM‐sensitive PAP enzymes (e.g. App1, Lpp1). Localization studies revealed that the majority of PAP activity resides in the membrane fraction, while the cytosolic fraction harbors only a small amount of activity. PAP a...
Source: Yeast - September 30, 2016 Category: Molecular Biology Authors: Derell Hardman, Daniel McFalls, Stylianos Fakas Tags: Research Article Source Type: research
