Evaluation of antimicrobial activity and probiotic properties of wild strain Pichia kudriavzevii isolated from frozen Idli batter
Abstract
The present research was undertaken to study the probiotic characteristics of Pichia kudriavzevii isolated from frozen idli batter. PCR amplification with 18S rRNA primers confirmed Pichia kudriavzevii, a xylose utilizing probiotic strain. It was resistant to physiological concentrations of bile salts, pepsin and pancreatic enzyme. It also showed efficient auto‐aggregation as well as co‐aggregation ability with 4 commercial probiotic yeasts and exhibited good hydrophobicity in xylene and toluene. The strain inhibited the growth of 13 enteropathogens and showed commensal relationship with 4 commercial probiotic...
Source: Yeast - May 31, 2016 Category: Molecular Biology Authors: Ramachandran Chelliah, Sudha Rani Ramakrishnan, Prince R. Prabhu, Usha Antony Tags: ISSY32 Special Issue Source Type: research
The catalytic subunit of the first mannosyltransferase in the GPI biosynthetic pathway affects growth, cell wall integrity, and hyphal morphogenesis in Candida albicans
Abstract
CaGpi14 is the catalytic subunit of the first mannosyltransferase that is involved in the GPI biosynthetic pathway in Candida albicans. We show that CaGPI14 is able to rescue a conditionally lethal gpi14 mutant of S. cerevisiae unlike its mammalian homolog. The depletion of this enzyme in C. albicans leads to severe growth defects, besides causing deficiencies in GPI anchor levels. In addition, CaGpi14 depletion results in cell wall defects and upregulation of the cell wall integrity response pathway. This in turn appears to trigger the osmotic stress dependent activation of the HOG1 pathway and an upregulation of...
Source: Yeast - May 31, 2016 Category: Molecular Biology Authors: Sneh Lata Singh, Ramesh Chandra Rai, Sudisht Kumar Sah, Sneha Sudha Komath Tags: ISSY32 Special Issue Source Type: research
Use of a fluoride channel as a new selection marker for fission yeast plasmids and application to fast genome editing with CRISPR/Cas9
Abstract
Fission yeast is a powerful model organism that has provided insights on important cellular processes thanks to the easiness of its genome editing by homologous recombination. However, creation of strains with a large number of targeted mutations or containing plasmids has been challenging because only a very small number of selection markers is available in S. pombe. In this paper, we identify two fission yeast fluoride exporter channels (Fex1p and Fex2p) and describe the development of a new strategy using Fex1p as a selection marker for transformants in rich media supplemented with fluoride. To our knowledge th...
Source: Yeast - May 31, 2016 Category: Molecular Biology Authors: Ronan Fernandez, Julien Berro Tags: Research Article Source Type: research
The halotolerant Debaryomyces hansenii, the Cinderella of non‐conventional yeasts
Abstract
Debaryomyces hansenii is a halotolerant yeast with a high biotechnological potential, particularly in food industry. However research in this yeast is limited by its molecular peculiarities. In this review we summarise the state of the art about research in this microorganisms, describing both pros and cons. We discuss (i) its halotolerance, (ii) the molecular factors involved in saline and osmotic stress, (iii) its high gene density and ambiguous CUG decoding, and (iv) its biotechnological and medical interests. We trust that all the bottlenecks in its study will be soon overcome and D. hansenii will become a fun...
Source: Yeast - May 31, 2016 Category: Molecular Biology Authors: Catarina Prista, Carmen Michán, Isabel M. Miranda, José Ramos Tags: Yeast Primer Source Type: research
Overexpression of the genes PDC1 and ADH1 activates glycerol conversion to ethanol in the thermotolerant yeast Ogataea (Hansenula) polymorpha
Abstract
Conversion of byproduct from biodiesel production glycerol to high‐value compounds is of great importance. Ethanol is considered as promising product of glycerol bioconversion. Methylotrophic thermotolerant yeast Ogataea (Hansenula) polymorpha is of great interest for this purpose as byproduct glycerol contains methanol and heavy metals as contaminants and this yeast utilizes methanol and is relatively resistant to heavy metals. Besides, O. polymorpha shows robust grows on glycerol and produces ethanol from different carbon sources. Thermotolerance of this yeast is an additional advantage that allows increasing ...
Source: Yeast - May 31, 2016 Category: Molecular Biology Authors: Iwona Kata, Marta V. Semkiv, Justyna Ruchala, Kostyantyn V. Dmytruk, Andriy A. Sibirny Tags: ISSY32 Special Issue Source Type: research
Outlining the influence of non ‐conventional yeasts in wine ageing over lees
Abstract
During the last decade, the use of innovative yeast cultures of both Saccharomyces cerevisiae and non‐Saccharomyces yeasts as alternative tools to manage the winemaking process have turned the oenology industry. Although the contribution of different yeast species to wine quality during fermentation is increasingly understood, information about their role in wine ageing over lees is really scarce. This work aims to analyse the incidence of three non‐Saccharomyces yeast species of oenological interest (Torulaspora delbrueckii, Lachancea thermotolerans and Metschnikowia pulcherrima) and of a commercial mannoprot...
Source: Yeast - May 15, 2016 Category: Molecular Biology Authors: Ignacio Belda, Eva Navascu és, Domingo Marquina, Antonio Santos, Fernando Calderón, Santiago Benito Tags: ISSY32 Special Issue Source Type: research
Outlining the influence of non‐conventional yeasts in wine ageing over lees
Abstract
During the last decade, the use of innovative yeast cultures of both Saccharomyces cerevisiae and non‐Saccharomyces yeasts as alternative tools to manage the winemaking process have turned the oenology industry. Although the contribution of different yeast species to wine quality during fermentation is increasingly understood, information about their role in wine ageing over lees is really scarce. This work aims to analyse the incidence of three non‐Saccharomyces yeast species of oenological interest (Torulaspora delbrueckii, Lachancea thermotolerans and Metschnikowia pulcherrima) and of a commercial mannoprot...
Source: Yeast - May 15, 2016 Category: Molecular Biology Authors: Ignacio Belda, Eva Navascués, Domingo Marquina, Antonio Santos, Fernando Calderón, Santiago Benito Tags: ISSY32 Special Issue Source Type: research
Proteomic analysis of Rhodotorula mucilaginosa: dealing with the issues of a non ‐conventional yeast
Abstract
Red yeasts ascribed to the species Rhodotorula mucilaginosa are gaining increasing attention, due to their numerous biotechnological applications, spanning carotenoid production, liquid bioremediation, heavy metal biotransformation and antifungal and plant growth‐promoting actions, but also for their role as opportunistic pathogens. Nevertheless, their characterization at the 'omic' level is still scarce. Here, we applied different proteomic workflows to R. mucilaginosa with the aim of assessing their potential in generating information on proteins and functions of biotechnological interest, with a particular fo...
Source: Yeast - May 12, 2016 Category: Molecular Biology Authors: Maria Filippa Addis, Alessandro Tanca, Sara Landolfo, Marcello Abbondio, Raffaela Cutzu, Grazia Biosa, Daniela Pagnozzi, Sergio Uzzau, Ilaria Mannazzu Tags: ISSY32 Special Issue Source Type: research
Discovery of genes involved in mitosis, cell division, cell wall integrity and chromosome segregation through construction of Schizosaccharomyces pombe deletion strains
Abstract
The fission yeast model system Schizosaccharomyces pombe is used to study fundamental biological processes. To continue to fill gaps in the S. pombe gene deletion collection, we constructed a set of 90 haploid gene deletion strains covering many previously uncharacterized genes. To begin to understand the function of these genes, we exposed this collection of strains to a battery of stress conditions. Using this information in combination with microscopy, proteomics and mini‐chromosome loss assays, we identified genes involved in cell wall integrity, cytokinesis, chromosome segregation and DNA metabolism. This s...
Source: Yeast - May 10, 2016 Category: Molecular Biology Authors: Jun‐Song Chen, Janel R. Beckley, Liping Ren, Anna Feoktistova, Michael A. Jensen, Nick Rhind, Kathleen L. Gould Tags: Genome Screen Reports Source Type: research
A stable phylogeny of the large ‐spored Metschnikowia clade
Abstract
Draft genomes of 55 strains representing all known large‐spored Metschnikowia species were used to construct a robust phylogeny of these yeasts found in association with flower‐visiting insects. The genomes were annotated with reference to Clavispora lusitaniae. From 3016 orthologues identified, 1061 were present in all strains with enough overlap to generate alignments of 500 bp or more. We constructed trees for all those alignments and evaluated their accuracy from their ability to resolve each of 22 sets of conspecifics correctly as sister taxa. Neighbour‐joining identified species membership better tha...
Source: Yeast - May 9, 2016 Category: Molecular Biology Authors: Marc ‐André Lachance, Emilia Hurtado, Tom Hsiang Tags: ISSY32 Special Issue Source Type: research
Insights from synthetic yeasts
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Source: Yeast - May 3, 2016 Category: Molecular Biology Authors: Damien Coudreuse Tags: Budding Topic Source Type: research
Issue Information ‐ Notes for Contribs p1
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Source: Yeast - May 3, 2016 Category: Molecular Biology Tags: Issue Information ‐ Notes for Contribs p1 Source Type: research
Issue Information ‐ Info Page
No abstract is available for this article. (Source: Yeast)
Source: Yeast - May 3, 2016 Category: Molecular Biology Tags: Issue Information ‐ Info Page Source Type: research
Issue Information ‐ TOC
No abstract is available for this article. (Source: Yeast)
Source: Yeast - May 3, 2016 Category: Molecular Biology Tags: Issue Information ‐ TOC Source Type: research
Social Wasp intestines host the local phenotypic variability of Saccharomyces cerevisiae strains
Abstract
Nowadays, the presence of Saccharomyces cerevisiae has been assessed in both wild and human‐related environments. Social wasps have been shown to maintain and vector S. cerevisiae among different environments. The availability of strains isolated from wasp intestines represents a striking opportunity to assess if the strains found in wasp intestines are characterized by peculiar traits. We analyzed strains isolated from social wasps’ intestine and compared them with strains isolated from other sources, all collected in a restricted geographic area. We evaluated the production of volatile metabolites during gra...
Source: Yeast - April 30, 2016 Category: Molecular Biology Authors: Leonardo Dapporto, Irene Stefanini, Damariz Rivero, Mario Polsinelli, Paolo Capretti, Paolo De Marchi, Roberto Viola, Stefano Turillazzi, Duccio Cavalieri Tags: ISSY32 Special Issue Source Type: research
