Evaluation of antimicrobial activity and probiotic properties of wild strain Pichia kudriavzevii isolated from frozen Idli batter

Abstract The present research was undertaken to study the probiotic characteristics of Pichia kudriavzevii isolated from frozen idli batter. PCR amplification with 18S rRNA primers confirmed Pichia kudriavzevii, a xylose utilizing probiotic strain. It was resistant to physiological concentrations of bile salts, pepsin and pancreatic enzyme. It also showed efficient auto‐aggregation as well as co‐aggregation ability with 4 commercial probiotic yeasts and exhibited good hydrophobicity in xylene and toluene. The strain inhibited the growth of 13 enteropathogens and showed commensal relationship with 4 commercial probiotic yeast and bacteria. Moreover, it was resistant to 30 antibiotics with different modes of action. The yeast exhibited thermo‐tolerance up to 95 °C for 2 h. The cell‐free supernatants were also found to be heat‐stable indicating the presence of thermo‐stable secondary metabolites. Hence, it could be exploited as starter culture, co‐culture or probiotic in the preparation of fermented products or incorporated in heat‐able foods as well.
Source: Yeast - Category: Molecular Biology Authors: Tags: ISSY32 Special Issue Source Type: research