Issue Information ‐ Notes for Contribs
No abstract is available for this article. (Source: Yeast)
Source: Yeast - October 10, 2017 Category: Molecular Biology Tags: Issue Information ‐ Notes for Contribs Source Type: research
Issue Information
No abstract is available for this article. (Source: Yeast)
Source: Yeast - October 10, 2017 Category: Molecular Biology Tags: Issue Information Source Type: research
The natural diversity and ecology of fission yeast.
Abstract
While the fission yeast is a powerful model of eukaryote biology, there have been few studies of quantitative genetics, phenotypic or genetic diversity. Here I survey the small collection of fission yeast diversity research. I discuss what we can infer about the ecology and origins of Schizosaccharomyces pombe from microbiology field studies and the few strains that have been collected. (Source: Yeast)
Source: Yeast - October 1, 2017 Category: Molecular Biology Authors: Daniel C. Jeffares Tags: BUDDING TOPIC Source Type: research
An efficient method for isolating mating ‐competent cells from bottom‐fermenting yeast using mating pheromone‐supersensitive mutants
Abstract
Crossbreeding is an effective approach to construct novel yeast strains with preferred characteristics; however, it is difficult to crossbreed strains of brewer's yeast, especially the bottom‐fermenting yeast Saccharomyces pastorianus, because of the relative inefficiency of the available methods to obtain mating‐competent cells (MCCs). Here, we describe a productive method for the isolation of MCCs without artificial genetic modification. We focused on the characteristics of two mating pheromone‐supersensitive (PS) mutants, Δbar1 and Δsst2, that show a growth defect in presence of the mating pheromone. Wh...
Source: Yeast - October 1, 2017 Category: Molecular Biology Authors: Taku Ota, Keiko Kanai, Hisami Nishimura, Satoshi Yoshida, Hiroyuki Yoshimoto, Osamu Kobayashi Tags: SPECIAL ISSUE ‐ YEAST INTERSPECIES HYBRIDS Source Type: research
On the catabolism of amino acids in the yeast Dekkera bruxellensis and the implications to industrial fermentation processes
Summary
In the last years several reports have reported the capacity of the yeast Dekkera (Brettanomyces) bruxellensis to survive and adapt to the industrial process of alcoholic fermentation. Much of this feature seems to relate to the ability to assimilate limiting sources of nutrients, or somehow some that are inaccessible to Saccharomyces cerevisiae, in particular the sources of nitrogen. Among them, amino acids (AA) are relevant in terms of beverage musts, and could also be important for bioethanol. In view of the limited knowledge on the control of AA, the present work combines physiological and genetic studies to un...
Source: Yeast - October 1, 2017 Category: Molecular Biology Authors: Denise Castro Parente, Danielli Batista Bezerra Cajueiro, Irina Charlot Pe ña Moreno, Fernanda Cristina Bezerra Leite, Will Barros Pita, Marcos Antonio Morais Tags: RESEARCH ARTICLE Source Type: research
The inheritance of mitochondrial DNA in interspecific Saccharomyces hybrids and their properties in winemaking
This study shows that mitotype restoration follows fixed schemata. We created isogenic, interspecific Saccharomyces cerevisiae x Saccharomyces uvarum hybrids through direct mating and analysed their mating frequency and mitotype. The mating frequency increased for most crosses in staggered mating. Mitotyping revealed that breeding lines with the same parental strain of S. cerevisiae and different parental strains of S. uvarum give rise to the same mitotype. According to our results, we postulate that the inheritance of mtDNA is dominated by one parental strain and that the superior mitotype is specific to each breeding lin...
Source: Yeast - October 1, 2017 Category: Molecular Biology Authors: Alexandra Verspohl, Samuele Pignedoli, Paolo Giudici Tags: SPECIAL ISSUE ‐ YEAST INTERSPECIES HYBRIDS Source Type: research
Behead and live long or the tale of cathepsin L
In this study we describe the establishment of an aging reporter that allows a reliable and relative quick screening of substances and genes that have an impact on the replicative lifespan. A cDNA library of the flatworm Dugesia tigrina that can be immortalized by beheading was screened using this aging reporter. Of all the flatworm genes, only one could be identified that significantly increased the replicative lifespan of S.cerevisiae. This gene is the cysteine protease cathepsin L that was sequenced for the first time in this study. We were able to show that this protease has the capability to degrade such proteins as t...
Source: Yeast - October 1, 2017 Category: Molecular Biology Authors: Maria Karolin Streubel, Johannes Bischof, Richard Weiss, Jutta Duschl, Wolfgang Liedl, Herbert Wimmer, Michael Breitenbach, Manuela Weber, Florian Geltinger, Klaus Richter, Mark Rinnerthaler Tags: RESEARCH ARTICLE Source Type: research
A Trichosporonales genome tree based on 27 haploid and three evolutionarily conserved ‘natural’ hybrid genomes
SUMMARY
To construct a backbone tree consisting of basidiomycetous yeasts, draft genome sequences from 25 species of Trichosporonales (Tremellomycetes, Basidiomycota) were generated. In addition to the hybrid genomes of Trichosporon coremiiforme and T. ovoides that we described previously, we identified an interspecies hybrid genome in Cutaneotrichosporon mucoides (formerly T. mucoides). This hybrid genome had a gene retention rate of approximately 55%, and its closest haploid relative was C. dermatis. After constructing the C. mucoides subgenomes, we generated a phylogenetic tree using genome data from the 27 haploid spec...
Source: Yeast - October 1, 2017 Category: Molecular Biology Authors: Masako Takashima, Sira Sriswasdi, Ri ‐ichiroh Manabe, Moriya Ohkuma, Takashi Sugita, Wataru Iwasaki Tags: SPECIAL ISSUE ‐ YEAST INTERSPECIES HYBRIDS Source Type: research
On the origins and industrial applications of Saccharomyces cerevisiae x Saccharomyces kudriavzevii hybrids
Summary
Companies based on alcoholic fermentation products, such as wine, beer, and biofuels, use yeasts to make their products. Each industrial process utilizes different media conditions, which differ in sugar content, the presence of inhibitors, and fermentation temperatures. Saccharomyces cerevisiae has traditionally been the main yeast responsible for most fermentation processes. However, the market is changing due to the consumer demands or external factors, such as climate change. Some processes, such as biofuel production or winemaking, require new yeasts to solve specific challenges, especially those associated wi...
Source: Yeast - October 1, 2017 Category: Molecular Biology Authors: David Peris, Roberto P érez‐Torrado, Chris Todd Hittinger, Eladio Barrio, Amparo Querol Tags: SPECIAL ISSUE ‐ YEAST INTERSPECIES HYBRIDS Source Type: research
Saccharomyces cerevisiae Gle2/Rae1 is involved in septin organization, essential for cell cycle progression
Abstract
Gle2/Rae1 is highly conserved from yeast to humans and has been described as an mRNA export factor. Additionally, it is implicated in the anaphase‐promoting complex‐mediated cell cycle regulation in higher eukaryotes. Here we identify an involvement for Saccharomyces cerevisiae Gle2 in septin organization, which is crucial for cell cycle progression and cell division. Gle2 genetically and physically interacts with components of the septin ring. Importantly, deletion of GLE2 leads to elongated buds, severe defects in septin‐assembly and their cellular mislocalization. Septin‐ring formation is triggered by t...
Source: Yeast - September 28, 2017 Category: Molecular Biology Authors: Gesa Zander, Wilfried Kramer, Anika Seel, Heike Krebber Tags: Research Article Source Type: research
The hybrid genomes of Saccharomyces pastorianus: A current perspective
Abstract
Saccharomyces pastorianus is a recently evolved interspecies hybrid of Saccharomyces cerevisiae and Saccharomyces eubayanus used in the production of lager‐type beers and has a long‐standing history with the brewing industry. At least two distinct types of lager yeasts (Groups I and II) have been identified based on chromosome content and structure. One important feature of the genomes of lager yeasts is the presence of a set of hybrid chromosomes that emerged as a result of homeologous recombination events between the parental chromosomes. The unique genetic composition of the hybrid genomes of S. pastorianus...
Source: Yeast - September 26, 2017 Category: Molecular Biology Authors: Chandre Monerawela, Ursula Bond Tags: Yeast Interspecies Hybrids Source Type: research
β‐1,6‐glucan synthesis‐associated genes are required for proper spore wall formation in Saccharomyces cerevisiae
Abstract
The yeast spore wall is an excellent model to study the assembly of an extracellular macromolecule structure. In the present study, mutants defective in β‐1,6‐glucan synthesis, including kre1∆, kre6∆, kre9∆ and big1∆, were sporulated to analyse the effect of β‐1,6‐glucan defects on the spore wall. Except for kre6∆, these mutant spores were sensitive to treatment with ether, suggesting that the mutations perturb the integrity of the spore wall. Morphologically, the mutant spores were indistinguishable from wild‐type spores. They lacked significant sporulation defects partly because the chitosa...
Source: Yeast - September 18, 2017 Category: Molecular Biology Authors: Hua ‐Ping Pan, Ning Wang, Hiroyuki Tachikawa, Hideki Nakanishi, Xiao‐Dong Gao Tags: Research Article Source Type: research
Issue Information ‐ Notes for Contribs
No abstract is available for this article. (Source: Yeast)
Source: Yeast - September 4, 2017 Category: Molecular Biology Tags: Issue Information ‐ Notes for Contribs Source Type: research
Issue Information
No abstract is available for this article. (Source: Yeast)
Source: Yeast - September 4, 2017 Category: Molecular Biology Tags: Issue Information Source Type: research
Modeling the contributions of chromosome segregation errors and aneuploidy to Saccharomyces hybrid sterility
Summary
Errors in meiosis can be important postzygotic barriers between different species. In Saccharomyces hybrids, chromosomal missegregation during meiosis I produces gametes with missing or extra chromosomes. Gametes with missing chromosomes are inviable, but we do not understand how extra chromosomes (disomies) influence hybrid gamete inviability. We designed a model predicting rates of missegregation in interspecific hybrid meioses assuming several different mechanisms of disomy tolerance, and compared predictions from the model to observations of sterility in hybrids between Saccharomyces yeast species. Sterility ob...
Source: Yeast - September 1, 2017 Category: Molecular Biology Authors: Primrose J. Boynton, Thijs Janzen, Duncan Greig Tags: RESEARCH ARTICLE Source Type: research
