Influence of phosphatidylserine and phosphatidylethanolamine on farnesol tolerance in Candida albicans

In this study, we found that loss of enzymes that synthesize the phospholipids phosphatidylserine (PS) and/or phosphatidylethanolamine (PE) compromise the tolerance of C. albicans to farnesol. Compared to wild‐type, the phospholipid mutant cho1∆/∆ (loss of PS and decreased PE synthesis) shows greater inhibition of growth, loss of ATP production, increased consumption of oxygen, and increased formation of reactive oxygen species (ROS) in the presence of farnesol (FOH). The cho1∆/∆ mutant also exhibits decreased sensitivity to mitochondrial ATPase inhibition, suggesting that cells lacking PS and/or downstream PE rely less on mitochondrial function for ATP synthesis. These data reveal that PS and PE play roles in farnesol tolerance and maintaining mitochondrial respiratory function.

Source: Yeast - November 16, 2017 Category: Molecular Biology Authors: Sahar Hasim, Elyse N. Vaughn, Dallas Donohoe, Donna M. Gordon, Susan Pfiffner, Todd B. Reynolds Tags: RESEARCH ARTICLE Source Type: research