Immunomodulatory role of reactive oxygen species and nitrogen species during T cell-driven neutrophil-enriched acute and chronic cutaneous delayed-type hypersensitivity reactions
Conclusions: MPO and NOX2 are the dominant sources of ROS/RNS in acute and chronic DTHR. Nevertheless, depletion of one primary source of ROS/RNS exhibited only marginal but conflicting impact on acute and chronic cutaneous DTHR. Thus, ROS/RNS are not a single entity, and each species has different properties at certain stages of the disease, resulting in different outcomes. (Source: Theranostics)
Source: Theranostics - January 15, 2021 Category: Molecular Biology Authors: Roman Mehling, Johannes Schwenck, Christina Lemberg, Christoph Trautwein, Laimdota Zizmare, Daniela Kramer, Anne M ü ller, Birgit Fehrenbacher, Irene Gonzalez-Menendez, Leticia Quintanilla-Martinez, Katrin Schr ö der, Ralph P. Brandes, Martin Schaller, Tags: Research Paper Source Type: research
Inter and intra-tumor somatostatin receptor 2 heterogeneity influences peptide receptor radionuclide therapy response
Conclusion: Heterogeneous SST2 expression levels within NETs cause differentially induced DNA damage levels, influence recurrent tumor phenotypes and impact the therapeutic response in different models and potentially in patients. Our results contribute to a better understanding of PRRT effects, which might impact future therapeutic outcome of NET patients. (Source: Theranostics)
Source: Theranostics - January 15, 2021 Category: Molecular Biology Authors: Danny Feijtel, Gabriela N. Doeswijk, Nicole S. Verkaik, Joost C. Haeck, Daniela Chicco, Carmelina Angotti, Mark W. Konijnenberg, Marion de Jong, Julie Nonnekens Tags: Research Paper Source Type: research
Highly sensitive magnetic particle imaging of vulnerable atherosclerotic plaque with active myeloperoxidase-targeted nanoparticles
Conclusions: This multimodal MPI approach revealed that active MPO-targeted nanoparticles might serve as a method for detecting vulnerable atherosclerotic plaques and monitoring MPO activity. (Source: Theranostics)
Source: Theranostics - January 15, 2021 Category: Molecular Biology Authors: Wei Tong, Hui Hui, Wenting Shang, Yingqian Zhang, Feng Tian, Qiang Ma, Xin Yang, Jie Tian, Yundai Chen Tags: Research Paper Source Type: research
Melanocortin 1 receptor attenuates early brain injury following subarachnoid hemorrhage by controlling mitochondrial metabolism via AMPK/SIRT1/PGC-1 α pathway in rats
Conclusions: The activation of MC1R with BMS-470539 significantly attenuated EBI after SAH by suppressing the oxidative stress, apoptosis, and mitochondrial fission through the AMPK/SIRT1/PGC-1α signaling pathway. (Source: Theranostics)
Source: Theranostics - January 15, 2021 Category: Molecular Biology Authors: Weilin Xu, Jun Yan, Umut Ocak, Cameron Lenahan, Anwen Shao, Jiping Tang, Jianmin Zhang, John H. Zhang Tags: Research Paper Source Type: research
Boiling histotripsy and in-situ CD40 stimulation improve the checkpoint blockade therapy of poorly immunogenic tumors
Conclusions: Data suggest that HT40 reprograms immunologically cold tumors and sensitizes them to ICI therapy. This approach may be clinically useful for treating advanced stage melanoma cancers. (Source: Theranostics)
Source: Theranostics - January 15, 2021 Category: Molecular Biology Authors: Mohit Pratap Singh, Sri Nandhini Sethuraman, Craig Miller, Jerry Malayer, Ashish Ranjan Tags: Research Paper Source Type: research
Targeting of glioma stem-like cells with a parthenolide derivative ACT001 through inhibition of AEBP1/PI3K/AKT signaling
Conclusions: Our data demonstrate AEBP1 as a new druggable target in GBM and ACT001 as a potential therapeutic option for improving the clinical treatment of GBM in combination with SHP099. (Source: Theranostics)
Source: Theranostics - January 15, 2021 Category: Molecular Biology Authors: Yanli Hou, Bowen Sun, Wenxue Liu, Bo Yu, Qiqi Shi, Fei Luo, Yongrui Bai, Haizhong Feng Tags: Research Paper Source Type: research
CXCR4 PET imaging of mantle cell lymphoma using [68Ga]Pentixafor: comparison with [18F]FDG-PET
Conclusion: [68Ga]Pentixafor-PET may become an alternative to [18F]FDG-PET in MCL patients, showing clearly higher detection rates and better tumor-to-background contrast. (Source: Theranostics)
Source: Theranostics - January 15, 2021 Category: Molecular Biology Authors: Marius E. Mayerhoefer, Markus Raderer, Wolfgang Lamm, Verena Pichler, Sarah Pfaff, Michael Weber, Barbara Kiesewetter, Markus Hacker, Lukas Kazianka, Philipp B. Staber, Hans-Juergen Wester, Johannes Rohrbeck, Ingrid Simonitsch-Klupp, Alexander Haug Tags: Research Paper Source Type: research
MRI-traceable theranostic nanoparticles for targeted cancer treatment
Current cancer therapies, including chemotherapy and radiotherapy, are imprecise, non-specific, and are often administered at high dosages - resulting in side effects that severely impact the patient's overall well-being. A variety of multifunctional, cancer-targeted nanotheranostic systems that integrate therapy, imaging, and tumor targeting functionalities in a single platform have been developed to overcome the shortcomings of traditional drugs. Among the imaging modalities used, magnetic resonance imaging (MRI) provides high resolution imaging of structures deep within the body and, in combination with other imaging mo...
Source: Theranostics - January 15, 2021 Category: Molecular Biology Authors: Tareq Anani, Shiva Rahmati, Nayer Sultana, Allan E. David Tags: Review Source Type: research
Cytosolic Ca2+ transients during pulsed focused ultrasound generate reactive oxygen species and cause DNA damage in tumor cells
Conclusions: Mechanotransduction of pFUS directly induces DNA damage in tumor cells by cytosolic Ca2+ transients causing formation of superoxide and subsequently, H2O2. These results further suggest potential clinical utility for pFUS. However, the lack of pFUS-induced DNA damage in C6 cells demonstrates a range of potential tumor responses that may arise from physiological differences such as Ca2+ or redox homeostasis. (Source: Theranostics)
Source: Theranostics - January 15, 2021 Category: Molecular Biology Authors: Robert B. Rosenblatt, Joseph A. Frank, Scott R. Burks Tags: Research Paper Source Type: research
Strategies in the delivery of Cas9 ribonucleoprotein for CRISPR/Cas9 genome editing
CRISPR/Cas9 genome editing has gained rapidly increasing attentions in recent years, however, the translation of this biotechnology into therapy has been hindered by efficient delivery of CRISPR/Cas9 materials into target cells. Direct delivery of CRISPR/Cas9 system as a ribonucleoprotein (RNP) complex consisting of Cas9 protein and single guide RNA (sgRNA) has emerged as a powerful and widespread method for genome editing due to its advantages of transient genome editing and reduced off-target effects. In this review, we summarized the current Cas9 RNP delivery systems including physical approaches and synthetic carriers....
Source: Theranostics - January 15, 2021 Category: Molecular Biology Authors: Song Zhang, Jiangtao Shen, Dali Li, Yiyun Cheng Tags: Review Source Type: research
Rapid design and development of CRISPR-Cas13a targeting SARS-CoV-2 spike protein
Conclusions: This study provides a rapid design pipeline for a CRISPR-Cas13a-based antiviral tool against SARS-CoV-2. Moreover, it offers a novel approach for anti-virus study even if the precise structures of viral proteins are indeterminate. (Source: Theranostics)
Source: Theranostics - January 15, 2021 Category: Molecular Biology Authors: Lin Wang, Junhu Zhou, Qixue Wang, Yunfei Wang, Chunsheng Kang Tags: Research Paper Source Type: research
Glioma stem cells and their roles within the hypoxic tumor microenvironment
Tumor microenvironments are the result of cellular alterations in cancer that support unrestricted growth and proliferation and result in further modifications in cell behavior, which are critical for tumor progression. Angiogenesis and therapeutic resistance are known to be modulated by hypoxia and other tumor microenvironments, such as acidic stress, both of which are core features of the glioblastoma microenvironment. Hypoxia has also been shown to promote a stem-like state in both non-neoplastic and tumor cells. In glial tumors, glioma stem cells (GSCs) are central in tumor growth, angiogenesis, and therapeutic resista...
Source: Theranostics - January 15, 2021 Category: Molecular Biology Authors: Nathaniel H. Boyd, Anh Nhat Tran, Joshua D. Bernstock, Tina Etminan, Amber B. Jones, G. Yancey Gillespie, Gregory K. Friedman, Anita B. Hjelmeland Tags: Review Source Type: research
Expansion of murine and human olfactory epithelium/mucosa colonies and generation of mature olfactory sensory neurons under chemically defined conditions
Conclusion: Our findings suggest that targeting expansion of olfactory epithelium/mucosa colonies in vitro potentially results in discovery of new source to cell replacement-based therapy against smell loss. (Source: Theranostics)
Source: Theranostics - January 15, 2021 Category: Molecular Biology Authors: Wenwen Ren, Li Wang, Xiujuan Zhang, Xiaoyu Feng, Liujing Zhuang, Nan Jiang, Rui Xu, Xuewen Li, Ping Wang, Xicai Sun, Hongmeng Yu, Yiqun Yu Tags: Research Paper Source Type: research
PLAGL2 promotes the proliferation and migration of gastric cancer cells via USP37-mediated deubiquitination of Snail1
Conclusions: In general, PLAGL2 promotes the proliferation and migration of GC cells through USP37-mediated deubiquitination of Snail1 protein. This work provided potential therapeutic targets for GC treatment. (Source: Theranostics)
Source: Theranostics - January 15, 2021 Category: Molecular Biology Authors: Liang Wu, Ning Zhao, Zili Zhou, Jinhuang Chen, Shengbo Han, Xudan Zhang, Haijun Bao, Wenzheng Yuan, Xiaogang Shu Tags: Research Paper Source Type: research
Brain-derived neurotrophic factor precursor in the immune system is a novel target for treating multiple sclerosis
Conclusion: The findings suggest that proBDNF from immune cells promotes the immunopathogenesis of MS. Monoclonal Ab-proB may be a promising therapeutic agent for treating MS. (Source: Theranostics)
Source: Theranostics - January 15, 2021 Category: Molecular Biology Authors: Zhao-Lan Hu, Cong Luo, Plinio Reinaldo Hurtado, Hui Li, Shuang Wang, Bo Hu, Jun-Mei Xu, Yang Liu, Shi-Qing Feng, Ernesto Hurtado-Perez, Kang Chen, Xin-Fu Zhou, Chang-Qi Li, Ru-Ping Dai Tags: Research Paper Source Type: research
