Ionizing radiation induces immediate protein acetylation changes in human cardiac microvascular endothelial cells
In this study, we analyzed the acute post-translational acetylation changes in primary human cardiac microvascular endothelial cells 4 h after a gamma radiation dose of 2 Gy. The acetylated peptides were enriched using anti-acetyl conjugated agarose beads. A total of 54 proteins were found to be altered in their acetylation status, 23 of which were deacetylated and 31 acetylated. Pathway analyses showed three protein categories particularly affected by radiation-induced changes in the acetylation status: the proteins involved in the translation process, the proteins of stress response, and mitochondrial proteins. The activ...
Source: Journal of Radiation Research - July 9, 2015 Category: Physics Authors: Barjaktarovic, Z., Kempf, S. J., Sriharshan, A., Merl-Pham, J., Atkinson, M. J., Tapio, S. Tags: Biology Source Type: research
Effects of dose rates on radiation-induced replenishment of intestinal stem cells determined by Lgr5 lineage tracing
In this study, we evaluated the effects of low-dose-rate radiation on stem cell maintenance. Tamoxifen (4OHT)-injected Lgr5-EGFP-IRES-CreERT2 x ROSA-LSL-LacZ mice were used, LacZ-labeled colonic crypts were enumerated, and the loss of LacZ+ crypts under low-dose-rate radiation was estimated. After 4OHT treatment, the number of LacZ-labeled Lgr5+ stem cells was higher in the colon of infant mice than in adult mice. The percentage of LacZ-labeled crypts in infant mice rapidly decreased after 4OHT treatment. However, the percentage of labeled crypts plateaued at ~2% at 4 weeks post-treatment and remained unchanged for up to 7...
Source: Journal of Radiation Research - July 9, 2015 Category: Physics Authors: Otsuka, K., Iwasaki, T. Tags: Biology Source Type: research
Abundance of BER-related proteins depends on cell proliferation status and the presence of DNA polymerase {beta}
In this study, we investigated the influence of POL β deficiency on MPG-initiated BER efficiency and the expression levels of BER-related proteins in log-phase and growth-arrested (G0) mouse embryonic fibroblasts (MEFs). G0 wild-type (WT) or POL β–deficient (Pol β–KO) cells showed greater resistance to methyl methanesulfonate than did log-phase cells, and repair of methylated bases was less efficient in the G0 cells. Apex1 mRNA expression was significantly lower in Pol β–KO or G0 WT MEFs than in log-phase WT MEFs. Moreover, although Mpg mRNA levels did not differ significantly among ce...
Source: Journal of Radiation Research - July 9, 2015 Category: Physics Authors: Yamamoto, M., Yamamoto, R., Takenaka, S., Matsuyama, S., Kubo, K. Tags: Biology Source Type: research
Incorporation of gantry angle correction for 3D dose prediction in intensity-modulated radiation therapy
Pretreatment dose verification with beam-by-beam analysis for intensity-modulated radiation therapy (IMRT) is commonly performed with a gantry angle of 0° using a 2D diode detector array. Any changes in multileaf collimator (MLC) position between the actual treatment gantry angle and 0° may result in deviations from the planned dose. We evaluated the effects of MLC positioning errors between the actual treatment gantry angles and nominal gantry angles. A gantry angle correction (GAC) factor was generated by performing a non-gap test at various gantry angles using an electronic portal imaging device (EPID). To conve...
Source: Journal of Radiation Research - May 11, 2015 Category: Physics Authors: Sumida, I., Yamaguchi, H., Kizaki, H., Aboshi, K., Tsujii, M., Yamada, Y., Yagi, M., Ogawa, K. Tags: Physics Source Type: research
Digital reconstructed radiography with multiple color image overlay for image-guided radiotherapy
Registration of patient anatomical structures to the reference position is a basic part of the patient set-up procedure. Registration of anatomical structures between the site of beam entrance on the patient surface and the distal target position is particularly important. Here, to improve patient positional accuracy during set-up for particle beam treatment, we propose a new visualization methodology using digitally reconstructed radiographs (DRRs), overlaid DRRs, and evaluation of overlaid DRR images in clinical cases. The overlaid method overlays two DRR images in different colors by dividing the CT image into two CT se...
Source: Journal of Radiation Research - May 11, 2015 Category: Physics Authors: Yoshino, S., Miki, K., Sakata, K., Nakayama, Y., Shibayama, K., Mori, S. Tags: Short Communication Source Type: research
Caudal epidural anesthesia during intracavitary brachytherapy for cervical cancer
It has been suggested that pain control during intracavitary brachytherapy for cervical cancer is insufficient in most hospitals in Japan. Our hospital began using caudal epidural anesthesia during high-dose-rate (HDR) intracavitary brachytherapy in 2011. The purpose of the present study was to retrospectively investigate the effects of caudal epidural anesthesia during HDR intracavitary brachytherapy for cervical cancer patients. Caudal epidural anesthesia for 34 cervical cancer patients was performed during HDR intracavitary brachytherapy between October 2011 and August 2013. We used the patients' self-reported Numeric R...
Source: Journal of Radiation Research - May 11, 2015 Category: Physics Authors: Isoyama-Shirakawa, Y., Nakamura, K., Abe, M., Kunitake, N., Matsumoto, K., Ohga, S., Sasaki, T., Uehara, S., Okushima, K., Shioyama, Y., Honda, H. Tags: Oncology Source Type: research
The threshold of hypothyroidism after radiation therapy for head and neck cancer: a retrospective analysis of 116 cases
The purpose of the present study was to determine the risk factors for developing thyroid disorders based on a dose–volume histograms (DVHs) analysis. Data from a total of 116 consecutive patients undergoing 3D conformal radiation therapy for head and neck cancers was retrospectively evaluated. Radiation therapy was performed between April 2007 and December 2010. There were 108 males and 8 females included in the study. The median follow-up term was 24 months (range, 1–62 months). The thyroid function was evaluated by measuring thyroid-stimulating hormone (TSH) and free thyroxine (FT4) levels. The mean thyroid ...
Source: Journal of Radiation Research - May 11, 2015 Category: Physics Authors: Fujiwara, M., Kamikonya, N., Odawara, S., Suzuki, H., Niwa, Y., Takada, Y., Doi, H., Terada, T., Uwa, N., Sagawa, K., Hirota, S. Tags: Oncology Source Type: research
Comparison of adverse effects of proton and X-ray chemoradiotherapy for esophageal cancer using an adaptive dose-volume histogram analysis
Cardiopulmonary late toxicity is of concern in concurrent chemoradiotherapy (CCRT) for esophageal cancer. The aim of this study was to examine the benefit of proton beam therapy (PBT) using clinical data and adaptive dose–volume histogram (DVH) analysis. The subjects were 44 patients with esophageal cancer who underwent definitive CCRT using X-rays (n = 19) or protons (n = 25). Experimental recalculation using protons was performed for the patient actually treated with X-rays, and vice versa. Target coverage and dose constraints of normal tissues were conserved. Lung V5–V20, mean lung dose (MLD), and heart V30&...
Source: Journal of Radiation Research - May 11, 2015 Category: Physics Authors: Makishima, H., Ishikawa, H., Terunuma, T., Hashimoto, T., Yamanashi, K., Sekiguchi, T., Mizumoto, M., Okumura, T., Sakae, T., Sakurai, H. Tags: Oncology Source Type: research
Survival outcomes after stereotactic body radiotherapy for 79 Japanese patients with hepatocellular carcinoma
Stereotactic body radiotherapy (SBRT) is a relatively new treatment for liver tumor. Outcomes of SBRT for liver tumors unsuitable for ablation or surgical resection were evaluated.
A total of 79 patients treated with SBRT for primary hepatocellular carcinoma (HCC) between 2004 and 2012 in six Japanese institutions were studied retrospectively. Patients treated with SBRT preceded by trans-arterial chemoembolization were eligible. Their median age was 73 years, 76% were males, and their Child–Pugh scores were Grades A (85%) and B (11%) before SBRT. The median biologically effective dose (α/β = 10 Gy) was 96....
Source: Journal of Radiation Research - May 11, 2015 Category: Physics Authors: Yamashita, H., Onishi, H., Murakami, N., Matsumoto, Y., Matsuo, Y., Nomiya, T., Nakagawa, K., Japanese Radiological Society multi-institutional SBRT study group (JRS-SBRTSG) Tags: Oncology Source Type: research
Lesion regression rate based on RECIST: prediction of treatment outcome in patients with head and neck cancer treated with chemoradiotherapy compared with FDG PET-CT
The aim of this study was to evaluate whether the lesion regression rate (LR) based on the Response Evaluation Criteria in Solid Tumors (RECIST) criteria could be used for the prediction of treatment outcome in head and neck squamous cell carcinoma (HNSCC) patients treated with chemoradiotherapy (CRT) compared with FDG PET-CT. A total of 33 patients underwent MRI and PET-CT at pretreatment and at 8 weeks after CRT. We assessed the treatment outcome by analyzing the following parameters: the RECIST criteria, LR, the European Organization for Research and Treatment of Cancer (EORTC) criteria, and pretreatment SUVmax of the p...
Source: Journal of Radiation Research - May 11, 2015 Category: Physics Authors: Matoba, M., Tuji, H., Shimode, Y., Kondo, T., Oota, K., Tonami, H. Tags: Oncology Source Type: research
Estimation of the total rectal dose of radical external beam and intracavitary radiotherapy for uterine cervical cancer using the deformable image registration method
In conclusion, the difference between the simple rectal dose–volume histogram (DVH) parameter addition and DIR-based cumulative rectal doses increased with decreasing DVH parameters. (Source: Journal of Radiation Research)
Source: Journal of Radiation Research - May 11, 2015 Category: Physics Authors: Hayashi, K., Isohashi, F., Akino, Y., Wakai, N., Mabuchi, S., Suzuki, O., Seo, Y., Ootani, Y., Sumida, I., Yoshioka, Y., Kimura, T., Ogawa, K. Tags: Oncology Source Type: research
A comparative planning study of step-and-shoot IMRT versus helical tomotherapy for whole-pelvis irradiation in cervical cancer
The aim of this study was to compare the dosimetric parameters of whole-pelvis radiotherapy (WPRT) for cervical cancer between step-and-shoot IMRT (SaS-IMRT) and Helical Tomotherapy™ (HT). Retrospective analysis was performed on 20 cervical cancer patients who received WPRT in our center between January 2011 and January 2014. SaS-IMRT and HT treatment plans were generated for each patient. The dosimetric values for target coverage and organ-at-risk (OAR) sparing were compared according to the criteria of the International Commission on Radiation Units and Measurements 83 (ICRU 83) guidelines. Differences in beam-on t...
Source: Journal of Radiation Research - May 11, 2015 Category: Physics Authors: Chitapanarux, I., Tharavichitkul, E., Nobnop, W., Wanwilairat, S., Vongtama, R., Traisathit, P. Tags: Oncology Source Type: research
A dosimetric comparison of two-phase adaptive intensity-modulated radiotherapy for locally advanced nasopharyngeal cancer
The purpose of this investigation was to evaluate the potential dosimetric benefits of a two-phase adaptive intensity-modulated radiotherapy (IMRT) protocol for patients with locally advanced nasopharyngeal cancer (NPC). A total of 17 patients with locally advanced NPC treated with IMRT had a second computed tomography (CT) scan after 17 fractions in order to apply and continue the treatment with an adapted plan after 20 fractions. To simulate the situation without adaptation, a hybrid plan was generated by applying the optimization parameters of the original treatment plan to the anatomy of the second CT scan. The dose&nd...
Source: Journal of Radiation Research - May 11, 2015 Category: Physics Authors: Chitapanarux, I., Chomprasert, K., Nobnaop, W., Wanwilairat, S., Tharavichitkul, E., Jakrabhandu, S., Onchan, W., Traisathit, P., Van Gestel, D. Tags: Oncology Source Type: research
Difference in distant failure site between locally advanced squamous cell carcinoma and adenocarcinoma of the uterine cervix after C-ion RT
In conclusion, there were high rates of distant failure after C-ion RT in AC cases regardless of PLN status, and there were high rates of distant failure after C-ion RT, especially PALN failure, in SqCC cases with positive PLNs. (Source: Journal of Radiation Research)
Source: Journal of Radiation Research - May 11, 2015 Category: Physics Authors: Wakatsuki, M., Kato, S., Ohno, T., Kiyohara, H., Karasawa, K., Tamaki, T., Ando, K., Irie, D., Shiba, S., Tsujii, H., Nakano, T., Kamada, T., Shozu, M., The Working Group of the Gynecological Tumor Tags: Oncology Source Type: research
Radioprotective effects of Sipunculus nudus L. polysaccharide combined with WR-2721, rhIL-11 and rhG-CSF on radiation-injured mice
This study investigated the radioprotective effect of Sipunculus nudus L. polysaccharide (SNP) in combination with WR-2721, rhIL-11 and rhG-CSF on irradiated mice. A total of 70 Imprinting Control Region (ICR) mice were divided into seven groups: the control group, the model group and five administration groups. All groups, except the control group, were exposed to a 5 Gy 60Co -ray beam. Blood parameters [including white blood cell (WBC), red blood cell (RBC) and platelet counts and hemoglobin level] were assessed three days before irradiation, and the on the 3rd, 7th and 14th days after irradiation. Spleen, thymus and tes...
Source: Journal of Radiation Research - May 11, 2015 Category: Physics Authors: Jiang, S., Shen, X., Liu, Y., He, Y., Jiang, D., Chen, W. Tags: Biology Source Type: research
