Methodology of drug screening and target identification for new necroptosis inhibitors
Publication date: Available online 27 November 2018Source: Journal of Pharmaceutical AnalysisAuthor(s): PengChao Pan, ZhenYu Cai, ChunLin Zhuang, XiaoFei Chen, YiFeng ChaiAbstractApoptosis is considered as the only form of regulated cell death for a long time. However, a novel form of programmed cell death called necroptosis was recently reported. The process of necroptosis is regulated and plays a critical role in the occurrence and development of multiple human diseases. Thus, the study of the molecular mechanism of necroptosis and its effective inhibitors has been an attractive field for researchers. Herein, we introduc...
Source: Journal of Pharmaceutical Analysis - November 28, 2018 Category: Drugs & Pharmacology Source Type: research
Recent advances in electrogenerated chemiluminescence biosensing methods for pharmaceuticals
Publication date: Available online 22 November 2018Source: Journal of Pharmaceutical AnalysisAuthor(s): Yu Zhang, Rui Zhang, Xiaolin Yang, Honglan Qi, Chengxiao ZhangAbstractElectrogenerated chemiluminescence (electrochemiluminescence, ECL) generates species at electrode surfaces, which undergoes electron-transfer reactions and forms excited states to emit light. It has become to a very powerful analytical technique and been widely used in such as clinic testing, biowarfare agent detection, and pharmaceutical analysis. This review focuses on the current trends of molecular recognition-based biosensing methods for pharmaceu...
Source: Journal of Pharmaceutical Analysis - November 23, 2018 Category: Drugs & Pharmacology Source Type: research
Development of a microcomposite with single-walled carbon nanotubes and Nd2O3 for determination of paracetamol in pharmaceutical dosage by adsorptive voltammetry
Publication date: Available online 22 November 2018Source: Journal of Pharmaceutical AnalysisAuthor(s): Verónica Arancibia, Johisner Penagos-Llanos, Edgar Nagles, Olimpo García-Beltrán, John J. HurtadoAbstractThis paper presents for the first time anew composite of carbon paste (CP), single-walled carbon nanotubes (SWCNTs) and Nd2O3 (NdOX). This versatile composite (NdOX-SWCNT/CPE) was applied to the oxidation of paracetamol (PCM). The newly formed surface was characterized by scanning electron microscopy (SEM), electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). The results showed greater conducti...
Source: Journal of Pharmaceutical Analysis - November 23, 2018 Category: Drugs & Pharmacology Source Type: research
New approaches of identification and characterization of tioconazole in raw material and in pharmaceutical dosage forms
Publication date: Available online 23 November 2018Source: Journal of Pharmaceutical AnalysisAuthor(s): Natalia L. Calvo, Vera A. Alvarez, María C. Lamas, Darío LeonardiAbstractTioconazole, a broad-spectrum antifungal agent, has significant activity against Candida albicans and other Candida species, and therefore, it is indicated for the topical treatment of superficial mycoses. The main goal of this work is to report an exhaustive identification and characterization procedure to improve and facilitate the on line quality control and continuous process monitoring of tioconzole in bulk material and loaded in two differen...
Source: Journal of Pharmaceutical Analysis - November 23, 2018 Category: Drugs & Pharmacology Source Type: research
Advances of supercritical fluid chromatography in lipid profiling
Publication date: Available online 17 November 2018Source: Journal of Pharmaceutical AnalysisAuthor(s): Yang Yang, Yanshan Liang, Jina Yang, Fengying Ye, Ting Zhou, Li GongkeAbstractSupercritical fluid chromatography (SFC) meets with great favor due to its high efficiency, low organic solvent consumption, and the specialty for the identification of the isomeric species. This review describes the advances of SFC in targeted and untargeted lipid profiling. The advancement of the SFC instruments and the stationary phases were summarized. Typical applications of SFC to the targeted and untargeted lipid profiling were discussed...
Source: Journal of Pharmaceutical Analysis - November 18, 2018 Category: Drugs & Pharmacology Source Type: research
Identification of α-Glucosidase Inhibitors from Clinacanthus nutans Leaf Extract using Liquid Chromatography-Mass Spectrometry-Based Metabolomics and Protein-Ligand Interaction with Molecular Docking
Publication date: Available online 15 November 2018Source: Journal of Pharmaceutical AnalysisAuthor(s): Suganya Murugesu, Zalikha Ibrahim, Qamar Uddin Ahmed, Bisha Fathamah Uzir, Nik Idris Nik Yusoff, Vikneswari Perumal, Faridah Abas, Khozirah Shaari, Alfi KhatibAbstractThe present study used in vitro and in silico techniques, as well as the metabolomics approach to characterise α-glucosidase inhibitors from different fractions of Clinacanthusnutans. C. nutansis a medicinal plant belonging to the Acanthaceae family, and is traditionally used to treat diabetes in Malaysia. n-Hexane, n-hexane: ethyl acetate (1:1, v/v), ethy...
Source: Journal of Pharmaceutical Analysis - November 16, 2018 Category: Drugs & Pharmacology Source Type: research
Spectrum-effect relationship between HPLC fingerprints and bioactive components of Radix Hedysari on increasing the peak bone mass of rat
This study is designed to investigate the bioactive components on increasing peak bone mass (PBM) by exploring the spectrum-effect relationship between chromatography fingerprints and effect. Multiple indicators are selected to evaluate the pharmacological activity. In fingerprints, 21 common peaks are obtained, five of which are identified. Furthermore, gray relational analysis (GRA) is a quantitative method of grey system theory and is used to describe the correlation degree of common peaks and pharmacological activities with relational value. 21 components are then divided into three different regions, of which ononin a...
Source: Journal of Pharmaceutical Analysis - November 2, 2018 Category: Drugs & Pharmacology Source Type: research
Development of nitrogen and sulfur-doped carbon dots for cellular imaging
In this study, a new synthetic method for nitrogen (N) and sulfur (S) -doped CDs was developed via a hydrothermal method using methionine and citric acid as raw materials. The s-prepared CDs exhibit excellent optical properties and good biocompatibility. The spherical N, S - doped CDs have an average diameter of 5 nm. They consist of C, O, N and S elements, and take on excellent water solubility due to the hydroxyl and carboxyl, amino groups on the surface. The CDs have a photoluminescence quantum yield of 13.8% using quinine sulfate as a reference, and the average fluorescence lifetime of CDs is 3.67 ns. The CDs...
Source: Journal of Pharmaceutical Analysis - October 16, 2018 Category: Drugs & Pharmacology Source Type: research
Qualitative and semi-quantitative analysis of health-care pharmaceutical products using laser-induced breakdown spectroscopy
Publication date: Available online 15 October 2018Source: Journal of Pharmaceutical AnalysisAuthor(s): Sobia Nisar, Ghulam Dastgeerb, Muhammad Shafiqa, Muhammad UsmanAbstractLaser-induced breakdown spectroscopy (LIBS) is a sensitive optical technique that is capable of rapid multi-elemental analysis. The development of this technique for elemental analysis of pharmaceutical products may eventually revolutionize the field of human health. Under normal circumstances, the elemental analysis of pharmaceutical products based on chemical methods is time-consuming and complicated. In this investigation, the principal aim is to de...
Source: Journal of Pharmaceutical Analysis - October 16, 2018 Category: Drugs & Pharmacology Source Type: research
Study of the compatibility of rubber stoppers for recombinant antitumor-antivirus protein injection by gas chromatography-mass spectrometry
Publication date: Available online 15 October 2018Source: Journal of Pharmaceutical AnalysisAuthor(s): Zhaorui Meng, Xun Gao, Haifeng Yu, Lan Zhang, Xiangyong Yu, Longshan ZhaoAbstractA simple, rapid, and sensitive gas chromatography-mass spectrometry (GC-MS) method was developed and validated for the simultaneous determination of two fatty acids, methyl hexadecanoate (MH), and methyl stearate (MS), to allow the evaluation of packaging-drug compatibility. The two migrants were quantified in selective ion-monitoring mode, with limits of detection (LOD) of 0.0030 μg/mL and 0.0121 μg/mL. Linear calibration curves ...
Source: Journal of Pharmaceutical Analysis - October 16, 2018 Category: Drugs & Pharmacology Source Type: research
Silymarin-laden PVP-PEG polymeric composite for enhanced aqueous solubility and dissolution rate: Preparation and in vitro characterization
Publication date: Available online 19 September 2018Source: Journal of Pharmaceutical AnalysisAuthor(s): Abid Mehmood Yousaf, Usman Rashid Malik, Yasser Shahzad, Tariq Mahmood, Talib HussainAbstractThe intent of this work was to develop, optimize and characterize a silymarin-laden polyvinylpyrrolidone (PVP)-polyethylene glycol (PEG) polymeric composite to resolve low aqueous solubility and dissolution rate problem of the drug. A number of silymarin-laden polymeric formulations were fabricated with different quantities of PVP K-30 and PEG 6000 via the solvent-evaporation method. The effect of PVP K-30 and PEG 6000 on the aq...
Source: Journal of Pharmaceutical Analysis - September 21, 2018 Category: Drugs & Pharmacology Source Type: research
Development of a competitive enzyme-linked immunosorbent assay for therapeutic drug monitoring of afatinib
This study sought to develop a simple and sensitive competitive enzyme-linked immunosorbent assay (ELISA) to quantify afatinib in plasma for routine pharmacokinetic applications. An anti-afatinib antibody was obtained using (S)-N-4-(3-chloro-4-fluorophenyl)-7-(tetrahydrofuran-3-yloxy)-quinazoline-4,6-diamine (CTQD), which has the same substructure as afatinib, as a hapten. Enzyme labeling of afatinib with horseradish peroxidase was similarly performed using CTQD. A simple competitive ELISA for afatinib was developed based on the principle of direct competition between afatinib and the enzyme marker for the anti-afatinib an...
Source: Journal of Pharmaceutical Analysis - September 19, 2018 Category: Drugs & Pharmacology Source Type: research
Qualitative and Quantitative Assessment of Related Substances in the Compound Ketoconazole and Clobetasol Propionate Cream by HPLC-TOF-MS and HPLC
This study could provide creative idea for routine analysis of quality control of the Compound Ketoconazole and Clobetasol Propionate Cream. (Source: Journal of Pharmaceutical Analysis)
Source: Journal of Pharmaceutical Analysis - September 12, 2018 Category: Drugs & Pharmacology Source Type: research
Assay development for determination of DZ2002, a new reversible SAHH inhibitor, and its acid metabolite DZA in blood and application to rat pharmacokinetic study
Publication date: Available online 7 September 2018Source: Journal of Pharmaceutical AnalysisAuthor(s): Weiwei Jia, Jing Li, Feifei Du, Yan Sun, Fang Xu, Fengqing Wang, Olajide E. Olaleye, Danghui Chen, Wei Tang, Jianping Zuo, Chuan LiAbstractMethyl (S)−4-(6-amino-9H-purin-9-yl)−2-hydroxybutanoate (DZ2002) is a potent reversible inhibitor of S-adenosyl-L-homocysteine hydrolase (SAHH). Due to its ester structure, DZ2002 is rapidly hydrolyzed in rat blood to 4-(6-amino-9H-purin-9-yl)−2-hydroxybutyric acid (DZA) during and after blood sampling from rats; this hampers accurate determination of the circulating DZ2002 and ...
Source: Journal of Pharmaceutical Analysis - September 7, 2018 Category: Drugs & Pharmacology Source Type: research
3D biofabrication of vascular networks for tissue regeneration: A report on recent advances
This article briefly reviews recent progress in 3D fabrication approaches used to fabricate vascular networks with incorporated cells, angiogenic factors, proteins, and/or peptides. The influence of the fabricated network on blood vessel formation, and the various features, merits, and shortcomings of the various fabrication techniques are discussed and summarized.Graphical abstract (Source: Journal of Pharmaceutical Analysis)
Source: Journal of Pharmaceutical Analysis - August 29, 2018 Category: Drugs & Pharmacology Source Type: research
