Study on screening potential allergenic proteins from infant milk powders based on human mast cell membrane chromatography and histamine release assays
Publication date: Available online 25 August 2018Source: Journal of Pharmaceutical AnalysisAuthor(s): Ping Zhang, Yingdi Shi, Xiaoshuang He, Wei Sun, Yanni Lv, Xiaofang HouAbstractCow's milk allergy is mainly observed in infants and young children. Most allergic reactions affect the skin, followed by the gastrointestinal and respiratory systems. Conventional diagnosis is based on positive allergy studies and evaluation of parameters including IgE and IgG1 levels, acute allergic skin response and anaphylactic shock reactions. We developed a cell membrane chromatographic method based on human mast cells (HMC-1) for screening...
Source: Journal of Pharmaceutical Analysis - August 26, 2018 Category: Drugs & Pharmacology Source Type: research
Discoursing on Soxhlet extraction of ginseng using association analysis and scanning electron microscopy
In this study, Panax ginseng was selected as a model sample. Soxhlet extraction by means of a water bath, which has always been neglected, was identified as a novel key factor in the poor repeatability in different batches of Soxhlet extraction, as it can affect the siphon times and reflux times, which have been positively correlated with the ginsenoside contents. By substituting round bottom flasks in the same column, the relative standard deviation of the most fluctuated ginsenoside, Rb1, was decreased from 24.6% to 5.02%. Scanning electron microscopy analysis confirmed that the breakdown of the surface of the ginseng po...
Source: Journal of Pharmaceutical Analysis - August 24, 2018 Category: Drugs & Pharmacology Source Type: research
Preface for Advances in Pharmaceutical Analysis 2017
Publication date: August 2018Source: Journal of Pharmaceutical Analysis, Volume 8, Issue 4Author(s): Zilin Chen (Source: Journal of Pharmaceutical Analysis)
Source: Journal of Pharmaceutical Analysis - August 18, 2018 Category: Drugs & Pharmacology Source Type: research
Study on degradation kinetics of epalrestat in aqueous solutions and characterization of its major degradation products under stress degradation conditions by UHPLC-PDA-MS/MS
Publication date: Available online 15 August 2018Source: Journal of Pharmaceutical AnalysisAuthor(s): Suyan Liu, Hong Sun, Xun Gao, Zhili Xiong, Zhonggui He, Longshan ZhaoAbstractDrug stability is closely related to drug safety and needs to be considered in the process of drug production, package and storage. To investigate the stability of epalrestat, a carboxylic acid derivative, a reversed-phase high-performance liquid chromatography (RP-HPLC) method was developed in this study and applied to analyze the degradation kinetics of epalrestat in aqueous solutions in various conditions, such as different pH, temperatures, io...
Source: Journal of Pharmaceutical Analysis - August 15, 2018 Category: Drugs & Pharmacology Source Type: research
Mass spectrometry detection of basic drugs in fast chiral analyses with vancomycin stationary phases
In this study, mobile phase conditions suitable for chiral analyses with electrospray ionization LC-MS were systematically investigated using vancomycin as a representative CSP. The performance of a 2.7 µm SPP based vancomycin CSP (SPP-V) 10 ×0.21-cm column was compared to that of a corresponding 5 µm fully porous particles based analogue column. The results demonstrated that the SPP-V column provides higher efficiencies, 2 to 5 time greater sensitivity and shorter analysis times for a set of 22 basic pharmaceutical drugs. The SPP-V was successfully applied for the analysis of the degradation products of racem...
Source: Journal of Pharmaceutical Analysis - August 9, 2018 Category: Drugs & Pharmacology Source Type: research
Recent advances in screening of enzymes inhibitors based on capillary electrophoresis
Publication date: Available online 11 May 2018Source: Journal of Pharmaceutical AnalysisAuthor(s): Mengxia Cheng, Zilin ChenAbstractCapillary electrophoresis with many advantages plays an important role in pharmaceutical analysis and drug screening. This review gives an overview on the recent advances in the developments and applications of capillary electrophoresis in the field of enzyme inhibitor screening. The period covers 2013 to 2017. Both the pre-capillary assay and in-capillary enzyme assay which includes electrophoretically mediated microanalysis (EMMA) and immobilized enzyme microreactor (IMER) are summarized in ...
Source: Journal of Pharmaceutical Analysis - July 27, 2018 Category: Drugs & Pharmacology Source Type: research
Preface
Publication date: Available online 21 July 2018Source: Journal of Pharmaceutical AnalysisAuthor(s): Zilin Chen (Source: Journal of Pharmaceutical Analysis)
Source: Journal of Pharmaceutical Analysis - July 21, 2018 Category: Drugs & Pharmacology Source Type: research
Advances in Tumor-Endothelial Cells Co-culture and Interaction on Microfluidics
Publication date: Available online 17 July 2018Source: Journal of Pharmaceutical AnalysisAuthor(s): Weiwei Li, Mashooq Khan, Sifeng Mao, Shuo Feng, Jin-Ming LinAbstractThe metastasis in which the cancer cells degrade the extracellular matrix (ECM) and invade to the surrounding and far tissues of the body is the leading causes of mortality in cancer patients. With a lot of advancement in the field, yet the biological causes of metastasis are poorly understood. The microfluidic system provides advanced technology to reconstruct a variety of in vivo-like environments for studying the interactions between tumor cells (TCs) and...
Source: Journal of Pharmaceutical Analysis - July 18, 2018 Category: Drugs & Pharmacology Source Type: research
Duplex microRNAs assay based on target-triggered universal reporter hybridization
Publication date: Available online 11 July 2018Source: Journal of Pharmaceutical AnalysisAuthor(s): Yinan Wang, Yue Sun, Choiwan Lau, Jianzhong LuAbstractIn this paper, we designed and evaluated a duplex detection strategy for microRNAs (miRNAs) using universal probe-based target-triggered double hybridization and fluorescent microsphere-based assay system (xMAP array). In the absence of target miRNA, reporter DNA cannot hybridize stably with the immobilized capture DNA, due to its low melting temperature. Only after adding target miRNA, reporter probe can hybridize with capture probe to form a stable three-component compl...
Source: Journal of Pharmaceutical Analysis - July 12, 2018 Category: Drugs & Pharmacology Source Type: research
Highly sensitive LC–MS/MS method to estimate doxepin and its metabolite nordoxepin in human plasma for a bioequivalence study
Publication date: Available online 16 June 2017Source: Journal of Pharmaceutical AnalysisAuthor(s): Nirav P. Patel, Mallika Sanyal, Naveen Sharma, Dinesh S. Patel, Pranav S. Shrivastav, Bhavin N. PatelAbstractA selective, sensitive and rugged liquid chromatography–tandem mass spectrometry (LC–MS/MS) assay has been developed for the simultaneous determination of doxepin (Dox) and its pharmacologically active metabolite, nordoxepin (NDox) in human plasma. The analytes and their internal standards (IS) were extracted from 500 µL of human plasma by liquid-liquid extraction using methyl tert-butyl ether. Chromatograph...
Source: Journal of Pharmaceutical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research
Novel ligand-based docking; molecular dynamic simulations; and absorption, distribution, metabolism, and excretion approach to analyzing potential acetylcholinesterase inhibitors for Alzheimer's disease
In conclusion, our study demonstrates that rutin is a superior drug candidate for AD. Therefore, we propose that this molecule is worth further investigation using in vitro studies. (Source: Journal of Pharmaceutical Analysis)
Source: Journal of Pharmaceutical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research
Solution pH jump during antibody and Fc-fusion protein thaw leads to increased aggregation
Publication date: Available online 18 September 2017Source: Journal of Pharmaceutical AnalysisAuthor(s): Kevin P. Kent, Chad E. Schroeder, Chandana SharmaAbstractFreeze-thaw cycles impact the amount of aggregation observed in antibodies and Fc-fusion proteins. Various formulation strategies are used to mitigate the amount of aggregation that occurs upon putting a protein solution through a freeze-thaw cycle. Additionally, low pH solutions cause native antibodies to unfold, which are prone to aggregate upon pH neutralization. There is great interest in the mechanism that causes therapeutic proteins to aggregate since aggreg...
Source: Journal of Pharmaceutical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research
Long-term stability of gentamicin sulfate-ethylenediaminetetraacetic acid disodium salt (EDTA-Na2) solution for catheter locks
In this study, fabrication, conditioning and sterilization processes of antimicrobial lock solution (ALS) were detailed and completed by a stability study. Stability of ALS was conducted for 12 months in vial (25 °C ± 2 °C, 60% ± 5% relative humidity (RH), and at 40 °C ± 2 °C, RH 75% ± 5%) and for 24 h and 72 h in TIVAP (40 °C ± 2 °C, RH 75% ± 5%). A stability indicating HPLC assay with UV detection for simultaneous quantification of gentamicin sulfate and EDTA-Na2 was developed. ALS was assayed by ion-pairing high performance liquid chromatography (HPLC) needing gentamic...
Source: Journal of Pharmaceutical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research
Detection and determination of undeclared synthetic caffeine in weight loss formulations using HPLC-DAD and UHPLC-MS/MS
Publication date: Available online 12 December 2017Source: Journal of Pharmaceutical AnalysisAuthor(s): Carine Viana, Gabriela M. Zemolin, Thaís R. Dal Molin, Luciana Gobo, Sandra Maria Ribeiro, Gabriela C. Leal, Gabriela Z. Marcon, Leandro M. de CarvalhoAbstractCaffeine is present in products marketed for weight loss, with the purpose of increasing thermogenesis and lipid metabolism. The dosage declared by the product manufacturer, or even its presence, is not always correctly described on the label. This work aimed to investigate the undeclared synthetic caffeine in weight loss formulations by a high-performance liquid ...
Source: Journal of Pharmaceutical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research
Cytotoxic effect of Rosa canina extract on human colon cancer cells through repression of telomerase expression
Publication date: Available online 15 December 2017Source: Journal of Pharmaceutical AnalysisAuthor(s): Ibrahim Turan, Selim Demir, Kagan Kilinc, Serap Ozer Yaman, Sema Misir, Hanife Kara, Berna Genc, Ahmet Mentese, Yuksel Aliyazicioglu, Orhan DegerAbstractRosa canina is a member of the genus Rosa that has long been used for medical objectives. Several studies have reported cytotoxic effects of different Rosa species, but there has been only limited investigation of the cytotoxic effect of R. canina. The purpose of the current study was to examine the potential effect of R. canina extract on cell viability, the cell cycle,...
Source: Journal of Pharmaceutical Analysis - July 10, 2018 Category: Drugs & Pharmacology Source Type: research
