Mass spectrometry detection of basic drugs in fast chiral analyses with vancomycin stationary phases

In this study, mobile phase conditions suitable for chiral analyses with electrospray ionization LC-MS were systematically investigated using vancomycin as a representative CSP. The performance of a 2.7 µm SPP based vancomycin CSP (SPP-V) 10 ×0.21-cm column was compared to that of a corresponding 5 µm fully porous particles based analogue column. The results demonstrated that the SPP-V column provides higher efficiencies, 2 to 5 time greater sensitivity and shorter analysis times for a set of 22 basic pharmaceutical drugs. The SPP-V was successfully applied for the analysis of the degradation products of racemic citalopram whose enantiomers could be selectively identified by MS.
Source: Journal of Pharmaceutical Analysis - Category: Drugs & Pharmacology Source Type: research