Offline two-dimensional liquid chromatography coupled with ion mobility-quadrupole time-of-flight mass spectrometry enabling four-dimensional separation and characterization of the multicomponents from white ginseng and red ginseng
Publication date: Available online 3 November 2019Source: Journal of Pharmaceutical AnalysisAuthor(s): Tiantian Zuo, Chunxia Zhang, Weiwei Li, Hongda Wang, Ying Hu, Wenzhi Yang, Li Jia, Xiaoyan Wang, Xiumei Gao, Dean GuoAbstractInherent complexity of plant metabolites necessitates the use of multi-dimensional information to accomplish comprehensive profiling and confirmative identification. A dimension-enhanced strategy, by offline two-dimensional liquid chromatography/ion mobility-quadrupole time-of-flight mass spectrometry (2D-LC/IM-QTOF-MS) enabling four-dimensional separations (2D-LC, IM, and MS), is proposed. In combi...
Source: Journal of Pharmaceutical Analysis - November 3, 2019 Category: Drugs & Pharmacology Source Type: research
Enantioseparation of FMOC-amino acids using an O-[2-(methacryloyloxy)-ethylcarbamoyl]-10,11-dihydroquinidine-silica hybrid monolithic column by nano-LC. Quantitation of lL-norvaline and lL-tryptophan in dietary supplements
Publication date: Available online 23 October 2019Source: Journal of Pharmaceutical AnalysisAuthor(s): Dongsheng Xu, Elena Sánchez-López, Qiqin Wang, Zhengjin Jiang, María Luisa MarinaAbstractAn analytical methodology based on an O-[2-(methacryloyloxy)-ethylcarbamoyl]-10,11-dihydroquinidine (MQD)-silica hybrid monolithic column was developed for the enantioseparation of 9-fluorenylmethoxycarbonyl (FMOC) derivatized amino acids by nano-liquid chromatography. The mobile phase was optimized including the apparent pH, content of ACN, and concentration of the buffer to obtain a satisfactory enantioresolution performance. 27 ...
Source: Journal of Pharmaceutical Analysis - October 24, 2019 Category: Drugs & Pharmacology Source Type: research
Editorial Board
Publication date: October 2019Source: Journal of Pharmaceutical Analysis, Volume 9, Issue 5Author(s): (Source: Journal of Pharmaceutical Analysis)
Source: Journal of Pharmaceutical Analysis - October 12, 2019 Category: Drugs & Pharmacology Source Type: research
Erucic acid from Isatis indigotica Fort. Suppresses influenza A virus replication and inflammation in vitro and in vivo through modulation of NF-κB and p38 MAPK pathway
Publication date: Available online 4 October 2019Source: Journal of Pharmaceutical AnalysisAuthor(s): Xiaoli Liang, Yuan Huang, Xiping Pan, Yanbing Hao, Xiaowei Chen, Haiming Jiang, Jing Li, Beixian Zhou, Zifeng YangAbstractIsatis indigotica Fort. (Ban-Lan-Gen) is an herbal medicine prescribed for influenza treatment. However, its active components and mode of action remain mostly unknown. In the present study, erucic acid was isolated from Isatis indigotica Fort., and subsequently its underlying mechanism against influenza A virus (IAV) infection was investigated in vitro and in vivo. Our results demonstrated that erucic ...
Source: Journal of Pharmaceutical Analysis - October 5, 2019 Category: Drugs & Pharmacology Source Type: research
Estimation of boswellic acids in herbal formulations containing Boswellia serrata extract and comprehensive characterization of secondary metabolites using UPLC-Q-Tof-MSe
In this study quantification of boswellic acids with UPLC-PDA method was performed as per the pharmacopeia guide lines. Furthermore, minor phytochemical constituents were identified and characterized with the help of LC-Q-Tof-MS/MS fragmentation data and various isoforms of boswellic acids and tirucallic acids in B. serrata oleo-gum-resin extract were identified.Graphical abstract (Source: Journal of Pharmaceutical Analysis)
Source: Journal of Pharmaceutical Analysis - October 2, 2019 Category: Drugs & Pharmacology Source Type: research
Nanodiamonds with powerful ability for drug delivery and biomedical applications: Recent updates on in vivo study and patents
Publication date: Available online 1 October 2019Source: Journal of Pharmaceutical AnalysisAuthor(s): Swati Chauhan, Neha Gulati Jain, Upendra NagaichAbstractNanodiamonds are novel nanosized carbon building blocks possessing varied fascinating mechanical, chemical, optical and biological properties making them significant active moiety carriers for biomedical application. These are known as the most ‘captivating’ crystals attributed to their chemical inertness and unique properties posing them useful for variety of applications in biomedical era. Alongside, it becomes increasingly important to find, ascertain and circu...
Source: Journal of Pharmaceutical Analysis - October 2, 2019 Category: Drugs & Pharmacology Source Type: research
Nuclear magnetic resonance based structure of the protoberberine alkaloid coralyne and its self association by spectroscopy techniques
Publication date: Available online 26 September 2019Source: Journal of Pharmaceutical AnalysisAuthor(s): Kumar Padmapriya, Ritu BarthwalAbstractCoralyne is an important alkaloid due to its anti cancer and other medicinal properties. It targets DNA in cells and acts as human topoisomerase-I poison, telomerase inhibitor and nucleic acid intercalator. It has high tendency to undergo self association, which is a matter of concern for therapeutic applications. The understanding of its interaction with DNA requires precise knowledge of chemical shifts in Nuclear Magnetic Resonance (NMR) spectra besides self association. The pres...
Source: Journal of Pharmaceutical Analysis - September 27, 2019 Category: Drugs & Pharmacology Source Type: research
Potential synergic mechanism of Wutou-Gancao herb-pair by inhibiting efflux transporter P-gp
In this study, potential synergic mechanism of the herb pair was investigated by utilizing multiple approaches. In silico and in vitro Caco-2 cell model were applied to study the potential binding mode of bioactive ingredients existing in liquorice with P-gp, as well as the inhibition effects on P-gp. Additionally, anti-inflammatory activity of aconitine (AC) combined with active ingredients of liquorice, as well as pharmacokinetic patterns of AC after co-administration were investigated. Anti-inflammatory effect of AC (1 mg/kg) in rats was enhanced in combination with bioactive ingredients of liquorice (10 mg/kg). I...
Source: Journal of Pharmaceutical Analysis - September 27, 2019 Category: Drugs & Pharmacology Source Type: research
Development of a UHPLC-MS/MS method for the quantification of ilaprazole enantiomers in rat plasma and its pharmacokinetic application
In this study, a specific and sensitive LC-MS/MS method has been developed and validated, for the quantification of ilaprazole enantiomers in the rat plasma, using R-lansoprazole as the internal standard. The enantioseparation was achieved on a CHIRALPAK AS-RH column (4.6 mm × 150 mm, i.d. 5 μm), with a mobile phase composed of 10 mM ammonium acetate aqueous solution and acetonitrile (60:40, v/v), at a flow-rate of 0.5 mL/min. The method was validated over the concentration range of 0.5–300 ng/mL for both, R- and S -ilaprazole. The lower limit of quantification was 0.5 ng/mL for both enantiomers. The ...
Source: Journal of Pharmaceutical Analysis - September 18, 2019 Category: Drugs & Pharmacology Source Type: research
Trace determination and characterization of ginsenosides in rat plasma through magnetic dispersive solid-phase extraction based on core-shell polydopamine-coated magnetic nanoparticles
This study presented a one-pot synthesis of magnetic polydopamine nanoparticles (Fe3O4@SiO2@PDA NPs) with multiple recognition sites for the magnetic dispersive solid-phase extraction (MDSPE) of ginsenosides from rat plasma treated with white ginseng. The extracted ginsenosides were characterized by combining an ultra-high-performance liquid chromatography coupled to a high-resolution mass spectrometry with supplemental UNIFI libraries. Response surface methodology was statistically used to optimize the extraction procedure of the ginsenosides. The reusability of Fe3O4@SiO2@PDA NPs was also examined and the results showed ...
Source: Journal of Pharmaceutical Analysis - September 6, 2019 Category: Drugs & Pharmacology Source Type: research
FITC labeling of human insulin and transport of FITC-Insulin conjugates through MDCK cell monolayer
Publication date: Available online 29 August 2019Source: Journal of Pharmaceutical AnalysisAuthor(s): Darshana Shah, Yuxing Guo, Joseph Ocando, Jun ShaoAbstractFluorescein isothiocyanate labeled insulin (FITC-Insulin) has been widely used for bioanalytical applications. Due to the high cost of commercial FITC-Insulin and tedious labeling procedures described in the literature, there is still a need to develop a cost effective, reliable and quick labeling method for insulin. The purpose of the present work was to develop a quick and affordable method for FITC labeling of human insulin and to determine the effect of differen...
Source: Journal of Pharmaceutical Analysis - August 30, 2019 Category: Drugs & Pharmacology Source Type: research
Quantitative analysis of 3-isopropylamino-1,2-propanediol as a degradation product of metoprolol in pharmaceutical dosage forms by HILIC-CAD
Publication date: Available online 28 August 2019Source: Journal of Pharmaceutical AnalysisAuthor(s): Qun Xu, Shane TanAbstractAryloxypropanolamine is an essential structural scaffold for a variety of β-adrenergic receptor antagonists such as metoprolol. Molecules with such a structural motif tend to degrade into α, β–hydroxypropanolamine impurities via a radical–initiated oxidation pathway. These impurities are typically polar and nonchromophoric, and are thus often overlooked using traditional reversed phase chromatography and UV detection. In this work, stress testing of metoprolol confirmed the generation of 3-i...
Source: Journal of Pharmaceutical Analysis - August 29, 2019 Category: Drugs & Pharmacology Source Type: research
Editorial Board
Publication date: August 2019Source: Journal of Pharmaceutical Analysis, Volume 9, Issue 4Author(s): (Source: Journal of Pharmaceutical Analysis)
Source: Journal of Pharmaceutical Analysis - August 13, 2019 Category: Drugs & Pharmacology Source Type: research
Preface for advances in pharmaceutical analysis 2018
Publication date: August 2019Source: Journal of Pharmaceutical Analysis, Volume 9, Issue 4Author(s): (Source: Journal of Pharmaceutical Analysis)
Source: Journal of Pharmaceutical Analysis - August 13, 2019 Category: Drugs & Pharmacology Source Type: research
Karacoline, identified by network pharmacology, reduces degradation of the extracellular matrix in intervertebral disc degeneration via the NF-κB signaling pathway
In this study, we selected key matrix metalloproteinases (MMPs), collagen II, and aggrecan as targets due to their association with intervertebral disc degeneration (IDD). Using these targets, we then used network pharmacology to predict a series of molecules that might exert therapeutic effects on IDD. Of these molecules, karacoline was predicted to have the best effect. Tumor necrosis factor (TNF)-α is known to promote the degeneration of the extracellular matrix in IDD. We therefore applied different concentrations of karacoline (0, 1.25, or 12.88 μM) along with 100 ng/mL TNF-α to rat nucleus pulposus cells and f...
Source: Journal of Pharmaceutical Analysis - July 26, 2019 Category: Drugs & Pharmacology Source Type: research
