Study on screening potential allergenic proteins from infant milk powders based on human mast cell membrane chromatography and histamine release assays

Publication date: Available online 25 August 2018Source: Journal of Pharmaceutical AnalysisAuthor(s): Ping Zhang, Yingdi Shi, Xiaoshuang He, Wei Sun, Yanni Lv, Xiaofang HouAbstractCow's milk allergy is mainly observed in infants and young children. Most allergic reactions affect the skin, followed by the gastrointestinal and respiratory systems. Conventional diagnosis is based on positive allergy studies and evaluation of parameters including IgE and IgG1 levels, acute allergic skin response and anaphylactic shock reactions. We developed a cell membrane chromatographic method based on human mast cells (HMC-1) for screening potential allergens in infant formula milk powders (IFMP). HMC-1 cell membranes were extracted and mixed with silica to prepare cell membrane chromatography columns (10 mm × 2 mm i.d., 5 μm). Under the conditions of 0.2 mL min-1 flow rate and 214 nm detection wavelength, human breast milk showed no retention. However, IFMP showed clear retention. The retained fractions were collected and analyzed through matrix-assisted laser desorption/ionization time of flight mass spectrometry. Four major milk proteins, α-casein, β-casein, α-lactalbumin, and β-lactoglobulin A were identified. Furthermore, these proteins and β-lactoglobulin B showed clear retention on HMC-1/CMC columns. To test the degranulation effects of the five proteins, histamine and β-hexosaminidase release assays were carried out. All five proteins induced HMC-1 cel...
Source: Journal of Pharmaceutical Analysis - Category: Drugs & Pharmacology Source Type: research