Issue Information
(Source: APMIS)
Source: APMIS - September 20, 2017 Category: Research Tags: Issue Information Source Type: research
The updated grading system of prostate carcinoma: an inter ‐observer agreement study among general pathologists in an academic practice
In 2016, the grading criteria for Gleason scoring (GS) have been updated in the WHO classification of tumors of the prostate, and a new set of grade groups (GG) was introduced. As the inter‐observer discordance is a well‐known concern in Gleason grading before the update and no reproducibility study testing the grade groups exists, we planned to evaluate the inter‐observer agreement of the most updated grading system. Four pathologists assessed 126 cores of prostatic carcinoma, and Kappa (k) test was calculated. The agreements for both GS and GG were substantial (k = 0.753 and 0.752; respectively). Discerning GG 2 fr...
Source: APMIS - September 15, 2017 Category: Research Authors: Areej M. Al Nemer, Tarek Elsharkawy, Mohamed Elshawarby, Dalal Al ‐Tamimi, Haitham Kussaibi, Ayesha Ahmed Tags: Original Article Source Type: research
Loss of regulatory characteristics in CD4+CD25+/hi T cells induced by impaired transforming growth factor beta secretion in pneumoconiosis
Pneumoconiosis is caused by the accumulation of airborne dust in the lung, which stimulates a progressive inflammatory response that ultimately results in lung fibrosis and respiratory failure. It is possible that regulatory cells in the immune system could function to suppress inflammation and possibly slow or reverse disease progression. However, results in this study suggest that in pneumoconiosis patients, the regulatory T cells (Tregs) and B cells are functionally impaired. First, we found that pneumoconiosis patients presented an upregulation of CD4+CD25+ T cells compared to controls, whereas the CD4+CD25+ and CD4+CD...
Source: APMIS - September 15, 2017 Category: Research Authors: Lu ‐Qin Bian, Ling Mao, Ying Bi, Shao‐Wei Zhou, Zi‐Dan Chen, Jun Wen, Jin Shi, Ling Wang Tags: Original Article Source Type: research
Clinicopathological study of lip cancer: a retrospective hospital ‐based study in Taiwan
To evaluate the clinicopathological characteristics, high‐risk lifestyle factors (HRLF: chronic exposure to sun, betel quid, alcohol, and tobacco), and prognostic factors of lip cancer. The hospital records of patients with pathologically confirmed lip squamous cell carcinoma (LSCC, n = 112) and lip basal cell carcinoma (LBCC, n = 21) were reviewed. Differences in clinicopathological characteristics between LSCC and LBCC, upper and lower lip, and status of second primary tumors were compared by chi‐square test and logistic regression. The prognostic factors for LSCC were analyzed by Cox regression. Compared with LB...
Source: APMIS - September 15, 2017 Category: Research Authors: Hui ‐Wen Tseng, Huei‐Han Liou, Kuo‐Wang Tsai, Luo‐Ping Ger, Yow‐Ling Shiue Tags: Original Article Source Type: research
Significance of histone methyltransferase SETDB1 expression in colon adenocarcinoma
This study investigated the clinical implications of SETDB1 (also known as KMT1E) in human colon adenocarcinoma. Expression levels of SETDB1 proteins were analyzed by immunohistochemistry staining, and tissue microarrays were used to examine expression profiles in human patients. Our results revealed that SETDB1 protein expression was significantly higher in tumor tissue than in normal tissue for the breast, colon, liver, and lung (p < 0.05). Moreover, an analysis with SurvExpress software suggested that elevated expression of SETDB1 mRNA was significantly associated with the overall survival of colon adenocarcinoma pat...
Source: APMIS - September 15, 2017 Category: Research Authors: Yi ‐Jung Ho, Yueh‐Min Lin, Yen‐Chi Huang, Jungshan Chang, Kun‐Tu Yeh, Liang‐In Lin, Zhiyuan Gong, Tsai‐Yu Tzeng, Jeng‐Wei Lu Tags: Original Article Source Type: research
A role of human beta defensin ‐1 in predicting prostatic adenocarcinoma in cases of false‐negative biopsy
The purpose of this study was to clarify the role of human beta defensin‐1 (hBD‐1) in predicting PAC in morphologically normal prostate glands. In total, 25 patients with a negative initial biopsy for PAC and diagnosed as PAC positive in subsequent biopsies performed within 1 year of the initial biopsy were included. As a control group, 22 patients negative for PAC in at least three consecutive histologic examinations were selected. Expression of hBD‐1 was analyzed separately via immunohistochemistry in paired cores of non‐neoplastic gland and PAC in the false‐negative group and control group. Loss of hBD‐1 exp...
Source: APMIS - September 8, 2017 Category: Research Authors: Soon Auck Hong, Ki Hong Kim, Tae Jin Lee, Eon Sub Park, Mi Kyung Kim, Soon Chul Myung Tags: Original Article Source Type: research
Low angiomotin ‐p130 with concomitant high Yes‐associated protein 1 expression is associated with adverse prognosis of advanced gastric cancer
In this study, a total of 166 patients with AGC were enrolled, and AMOT‐p130 and YAP1 levels were analyzed by immunohistochemistry using tissue microarrays. Low AMOT‐p130 together with high YAP1 expression (n = 30, 18.1%) was associated with high T stage (p = 0.042), high TNM stage (p = 0.025), and venous invasion (p = 0.048). A Kaplan–Meier survival analysis with log‐rank test revealed a significant correlation with decreased AMOT‐p130 coupled with high nuclear YAP1 expression with shorter overall survival (p = 0.0045) and disease‐free survival (p = 0.0028). Furthermore, multivariate analyses showed that the l...
Source: APMIS - September 8, 2017 Category: Research Authors: Soon Auck Hong, Myoung Won Son, Junhun Cho, Si ‐hyong Jang, Hyun Ju Lee, Ji‐Hye Lee, Hyun Deuk Cho, Mee‐Hye Oh, Moon Soo Lee Tags: Original Article Source Type: research
Effects of FCGRIIIa ‐158V/F polymorphism on antibody‐dependent cellular cytotoxicity activity of adalimumab
In this study, we analyzed healthy Japanese to clarify genotype frequency using a direct sequence method. In addition, we examined the association between the ADA‐mediated ADCC activity and the polymorphism. Our results showed that the frequencies of the V/V, V/F, and F/F genotypes in healthy Japanese were 9.2%, 39.8%, and 51.0%, respectively. The average activity of ADA‐mediated ADCC was 25.0%, 19.0%, and 13.3% in the V/V, V/F, and F/F genotypes, respectively. Then, the ADCC activity of V/V was significantly higher than that of F/F (p < 0.05) in therapeutic concentration. The differences in therapeutic effect of AD...
Source: APMIS - September 1, 2017 Category: Research Authors: Koji Kimura, Daigo Kobayashi, Saori Hatoyama, Mizuki Yamamoto, Risa Takayanagi, Yasuhiko Yamada Tags: Original Article Source Type: research
Acinetobacter baumannii isolated from hospital ‐acquired infection: biofilm production and drug susceptibility
In this study, we evaluate relation between these two factors. The biofilm formation was investigated in M63 medium with casein in microtiter plates, and the drug susceptibility was performed by disk diffusion methods. We found that 80–98% strains formed a biofilm. Strains showing sensitivity to amikacin and tobramycin from ICU produced more biofilm than strains showing resistance to these antibiotics. Ceftazidime‐sensitive strains formed a smaller biofilm than resistant. The logistic regression shows association between drug resistance and strains originating from ICU. In case of ceftazidime, strong biofilm formation ...
Source: APMIS - September 1, 2017 Category: Research Authors: Pawe ł Krzyściak, Agnieszka Chmielarczyk, Monika Pobiega, Dorota Romaniszyn, Jadwiga Wójkowska‐Mach Tags: Original Article Source Type: research
Biofilm formation of beta ‐hemolytic group G Streptococcus dysgalactiae subspecies equisimilis isolates and its association with emm polymorphism
Biofilm formation has been well known as a determinant of bacterial virulence. Group G Streptococcus dysgalactiae subspecies equisimilis (SDSE), a relevant pathogen with increasing medical importance, was evaluated for the biofilm‐forming potential. Microtiter plate assay was used to assess the most feasible medium for group G SDSE to form a biofilm. Among 246 SDSE isolates examined, 46.7%, 43.5%, 33.3%, and 26.4% of isolates showed moderate or strong biofilm‐forming abilities using tryptic soy broth (TSB), brain heart infusion broth (BHI), Todd‐Hewitt broth (THB), and C medium with 30 mM glucose (CMG), respectively....
Source: APMIS - September 1, 2017 Category: Research Authors: Jui ‐Shan Ma, Sin‐Yu Chen, Hsueh‐Hsia Lo Tags: Original Article Source Type: research
CCL2 recruits T cells into the brain in a CCR2 ‐independent manner
CCL2 is a chemokine that can be induced during neuroinflammation to recruit immune cells, but its role in the central nervous system (CNS) is unclear. Our aim was to better understand its role. We induced CCL2 in CNS of naive CCL2‐deficient mice using intrathecally administered replication‐defective adenovirus and examined cell infiltration by flow cytometry. CCL2 expression induced pronounced and unexpected recruitment of regulatory and IFNγ‐producing T cells to CNS from blood, possibly related to defective egress of monocytes from CCL2‐deficient bone marrow. Infiltration also occurred in mice lacking CCR2, a rec...
Source: APMIS - August 24, 2017 Category: Research Authors: Oriane C édile, Agnieszka Wlodarczyk, Trevor Owens Tags: Original Article Source Type: research
Issue Information
(Source: APMIS)
Source: APMIS - August 7, 2017 Category: Research Tags: Issue Information Source Type: research
The methylation status and expression of human telomerase reverse transcriptase is significantly high in oral carcinogenesis
In this study, we investigated the methylation and expression of hTERT in oral squamous cell carcinoma (OSCC), oral leukoplakia, and normal oral mucosa. Furthermore, we investigated the significance of hTERT to the clinicopathological findings of OSCC. 35 OSCC, 50 oral leukoplakia (epithelial dysplasia n = 25, squamous cell hyperplasia n = 25), and 10 normal oral mucosa samples were investigated through methylation‐specific PCR. Immunohistochemistry was analyzed in 35 OSCC, 50 oral leukoplakia, and 4 normal oral mucosa samples. The methylation and expression of hTERT increased from normal oral mucosa to oral leukoplakia ...
Source: APMIS - August 2, 2017 Category: Research Authors: Kazuya Haraguchi, Naomi Yada, Shinobu Sato, Manabu Habu, Mana Hayakawa, Osamu Takahashi, Masaaki Sasaguri, Shigeori Takenaka, Izumi Yoshioka, Kou Matsuo, Kazuhiro Tominaga Tags: Original Article Source Type: research
The expression profile and prognostic value of APE/Ref ‐1 and NPM1 in high‐grade serous ovarian adenocarcinoma
To analyze the expression trends and clinical significance of Apurinic/Apyrimidinic Endodeoxyribonuclease 1 (APE1/Ref‐1) and Nucleophosmin (NPM1) proteins in high‐grade serous ovarian adenocarcinoma (HGSC). The expressions of APE1/Ref‐1 and NPM1 proteins in 94 patients with HGSC were determined using the immunohistochemical (IHC) method, and their relationships with clinicopathological features were analyzed by the χ2 test or Fisher's exact test. The follow‐up data, Cox proportional hazards univariate and multivariate survival analyses were integrated to evaluate the prognostic factors affecting patients with HGSC...
Source: APMIS - August 2, 2017 Category: Research Authors: Xiaomei Fan, Lixuan Wen, Yuehong Li, Lei Lou, Weina Liu, Jun Zhang Tags: Original Article Source Type: research
Enhanced molecular typing of Treponema pallidum identified a new tp0548 Gene type in Shandong, China
(Source: APMIS)
Source: APMIS - August 2, 2017 Category: Research Authors: Zhen Li, Chuan Wang, Hailu Xiao, Wei Zhao, Zhongwei Li, Rongtao Zheng, Jianling Hou, Na Huang, Hongqing Tian Tags: Letter to the Editor Source Type: research
