Comparisons of neutrophil ‐, monocyte‐, eosinophil‐, and basophil‐ lymphocyte ratios among various systemic autoimmune rheumatic diseases
This study was aimed to evaluate levels of neutrophil‐ (NLR), monocyte‐ (MLR), eosinophil‐ (ELR), and basophil‐lymphocyte ratio (BLR) and their association with inflammatory markers in systemic autoimmune rheumatic diseases (SARDs). A total of 1139 SARD patients and 170 healthy individuals were enrolled. Clinical and laboratory data were extracted. NLR and MLR were significantly increased, but BLR decreased in most SARD patients (p < 0.05). ELR were significantly decreased in systemic lupus erythematosus (SLE) patients, but increased in those with other SARDs (p < 0.001). In SLE patients, C‐reactive pro...
Source: APMIS - August 1, 2017 Category: Research Authors: Zaixing Yang, Zhiyu Zhang, Feng Lin, Yingpeng Ren, Donghong Liu, Renqian Zhong, Yan Liang Tags: Original Article Source Type: research
Mycoplasma genitalium, an agent of reemerging sexually transmitted infections
We report here M. genitalium prevalence among the specimens received for STI diagnosis in our routine microbiological laboratory in the university hospital in Marseille, France. We tested 4 624 samples from 3 793 patients using qPCR for M. genitalium, C. trachomatis, N. gonorrheae, T. pallidum. Of these samples, 528 (13.6%) patients were tested positive for at least one pathogen and 126 (3.3%) were positive for M. genitalium. M. genitalium is the second most prevalent micro‐organism detected in women after C. trachomatis (10.4%) and the third most prevalent in men after C. trachomatis (5.1%) and N. gonorrhoeae (4.4%). We...
Source: APMIS - August 1, 2017 Category: Research Authors: Sophie Edouard, Herv é Tissot‐Dupont, Grégory Dubourg, Annick Bernard, Pierre‐Edouard Fournier, Isabelle Ravaux, Andreas Stein, Didier Raoult Tags: Original Article Source Type: research
Hyaluronate effect on bacterial biofilm in ENT district infections: a review
In conclusion, this paper underlines how the efficacy of hyaluronate as an anti‐bacterial biofilm agent is well demonstrated by in vitro studies; it is, however, only preliminarily demonstrated by clinical studies. (Source: APMIS)
Source: APMIS - July 24, 2017 Category: Research Authors: Alberto Vito Marcuzzo, Margherita Tofanelli, Francesca Boscolo Nata, Annalisa Gatto, Giancarlo Tirelli Tags: Review Article Source Type: research
Testing for high ‐risk HPV in cervical and tonsillar paraffin‐embedded tissue using a cartridge‐based assay
This study evaluates the suitability of Xpert HPV (Cepheid, Sunnyvale, CA, USA) test for cervical and tonsillar formalin‐fixed paraffin‐embedded (FFPE) tissue samples as compared to the tests currently used in diagnostics. Cervical biopsies and liquid cytology (LC) samples were collected from 48 women attending colposcopy. Biopsies were processed for histology and tested for hrHPV using Xpert HPV. LC samples were tested using Xpert and Hybrid Capture 2 (HC2; Qiagen, Hilden, Germany) tests. Also 29 archived tonsillar carcinoma samples were tested using Xpert, and the results were compared with histology and immunohistoc...
Source: APMIS - July 24, 2017 Category: Research Authors: Elina Virtanen, Pekka Laurila, Jaana Hagstr öm, Pekka Nieminen, Eeva Auvinen Tags: Original Article Source Type: research
No associations established between single nucleotide polymorphisms in human Toll ‐like receptor 2 and Toll‐interacting protein and Staphylococcus aureus bloodstream infections
Staphylococcus aureus bloodstream infections (SABSI) are associated with high morbidity and mortality. The Toll‐like receptor 2 (TLR2) and Toll‐interacting protein (TOLLIP) are important in recognition and regulation of human innate immunity response to S. aureus. Single nucleotide polymorphisms (SNPs) in the TLR2 and TOLLIP encoding genes have been associated with disease, including BSI. The aim of this study was to examine potential associations between a selection of SNPs in the genes encoding TLR2 and TOLLIP, and predisposition, severity, and outcome of SABSI. All patients ≥18 years of age with at least one S. au...
Source: APMIS - July 24, 2017 Category: Research Authors: Tom Eirik Smeland, Fredrik M üller, Anita Blomfeldt, Knut Stavem, Hege Vangstein Aamot Tags: Original Article Source Type: research
CXCL8 promotes the invasion of human osteosarcoma cells by regulation of PI3K/Akt signaling pathway
In conclusion, our findings suggested that CXCL8 enhanced the invasion and suppressed late stage apoptosis of osteosarcoma cells probably via influencing PI3K/Akt signaling pathway and elevating the expression of MMPs. CXCL8 may promote disease progression of osteosarcoma as a protumorigenic molecule, and may be served as a new therapeutic target for osteosarcoma. (Source: APMIS)
Source: APMIS - July 24, 2017 Category: Research Authors: Hai Jiang, Xiaowei Wang, Wusheng Miao, Bing Wang, Yusheng Qiu Tags: Original Article Source Type: research
Unrecognized viral infections and chromosome abnormalities as a cause of fetal death – examination with fluorescence in situ hybridization, immunohistochemistry and polymerase chain reaction
Fifteen to 50% of fetal deaths remain unexplained after post‐mortem examination depending on inclusion criteria and classification systems. Our aim was to examine a selection of unexplained fetal deaths in order to investigate whether any common chromosome aberrations or viral infections were present. Reports from 351 fetal autopsies performed at the Department of Pathology and Medical Genetics at St. Olavs University Hospital from 2001 through 2010 were reviewed. Of these, 105 fetal deaths were classified as unexplained. Tissue samples from 30 cases were further examined with fluorescence in situ hybridization (FISH) t...
Source: APMIS - July 24, 2017 Category: Research Authors: Bente Ediassen Opsj øn, Svein Arne Nordbø, Christina Vogt Tags: Original Article Source Type: research
Regulatory T cell subsets in peripheral blood of celiac disease patients and TLR2 expression: correlation with oxidative stress
This report highlights the effect of elevated oxidative stress in CD on reduced traffic of iTregs toward periphery. A strong correlation was observed between the cytokine IFN‐γ and TLR2 expression in movement of iTregs in CD patients. (Source: APMIS)
Source: APMIS - July 24, 2017 Category: Research Authors: Sanjay Kumar, Sadhna Lal, Archana Bhatnagar Tags: Original Article Source Type: research
Molecular characterization of nasal methicillin resistant Staphylococcus aureus isolates from workers of an automaker company in southeast Iran
Colonization of methicillin resistant Staphylococccus aureus (MRSA) can occur more commonly in healthy people who live in close together or are in close physical contact with each other. Having knowledge about the molecular characteristics of these strains provides considerable discernment into the epidemiology of this important microorganism. A total of 806 nasal swabs were collected from healthy workers of an automaker company in the southeast of Iran and were analyzed to detect MRSA isolates. Multilocus sequence typing (MLST), spa typing, and detection of staphylococcal cassette chromosome mec (SCCmec) were performed. T...
Source: APMIS - July 24, 2017 Category: Research Authors: Mohammad Hossein Sobhanipoor, Roya Ahmadrajabi, Afsaneh Karmostaji, Fereshteh Saffari Tags: Original Article Source Type: research
Tim ‐1+ B cells suppress T cell interferon‐gamma production and promote Foxp3 expression, but have impaired regulatory function in coronary artery disease
Atherosclerosis and its associated coronary artery disease (CAD) represent another chronic low‐grade inflammatory disorder. Regulatory B cells (Bregs) possess essential functions in maintaining peripheral tolerance and inhibiting pathogenic inflammation through IL‐10. Here, we investigated one subset of Bregs, Tim‐1+ B cell, and its role in atherosclerosis and CAD patients. In healthy individuals, IL‐10‐producing B cells were predominantly found in the Tim‐1+ B cells. Upon stimulation of the B cell receptor (BCR) and Toll‐like receptor 9 (TLR‐9) by anti‐BCR antibodies and CpG, respectively, the Tim‐1+ B...
Source: APMIS - July 24, 2017 Category: Research Authors: Xiao ‐Long Gu, Huan He, Lin Lin, Guo‐Xin Luo, Yan‐Fei Wen, Ding‐Cheng Xiang, Jian Qiu Tags: Original Article Source Type: research
Cytokine expression associated with Helicobacter pylori and Epstein ‐Barr virus infection in gastric carcinogenesis
Helicobacter pylori and Epstein‐Barr virus (EBV) infection, and associated cytokines are involved in gastric carcinogenesis. We investigated the expression of these cytokines and their relationship with clinicopathological characteristics. The study included specimens from 207 patients with gastric adenocarcinoma, 56 with chronic gastritis, 32 with metaplasia, and 30 with low‐grade epithelial dysplasia. Tissue microarrays were constructed and immunohistochemical staining for IL‐1β, IL‐6, IL‐10, IL‐17, p16, p21, TNF‐α, and TNFR1 was performed. EBV and H. pylori infection status was determined. IL‐1β, IL...
Source: APMIS - July 24, 2017 Category: Research Authors: Jung Yeon Kim, Byung ‐Noe Bae, Guhyun Kang, Hyun‐Jung Kim, Kyeongmee Park Tags: Original Article Source Type: research
BMP ‐7 ameliorates cobalt alloy particle‐induced inflammation by suppressing Th17 responses
Metal wear debris has been shown to activate an aseptic osteolytic process that causes failure in total joint arthroplasty (TJA). This osteolysis is characterized by a proinflammatory, self‐propagating immune response involving primarily macrophages, dendritic cells, and activated osteoclasts, as well as T cells and B cells. The human bone morphogenic protein (BMP)‐7, on the other hand, was shown to promote osteoblast survival, and reversed the downregulation of anabolic Smad proteins and Runx2 following cobalt injury. Therefore, we investigated the effect and mechanism of BMP‐7 on the proinflammatory immune response...
Source: APMIS - July 24, 2017 Category: Research Authors: Fengrong Chen, Ruisong Chen, Haoyuan Liu, Rupeng Sun, Jianming Huang, Zheyuan Huang, Guojian Jian Tags: Original Article Source Type: research
Issue Information
(Source: APMIS)
Source: APMIS - July 18, 2017 Category: Research Tags: Issue Information Source Type: research
First environmental sample containing plasmid ‐mediated colistin‐resistant ESBL‐producing Escherichia coli detected in Norway
This report illustrates that E. coli strains carrying plasmid‐mediated colistin resistance genes have also reached areas where this drug is hardly used at all. Surveillance of colistin resistance in environmental, veterinary, and human strains is warranted also in countries where colistin resistance is rare in clinical settings. (Source: APMIS)
Source: APMIS - June 22, 2017 Category: Research Authors: Silje Bakken J ørgensen, Arne Søraas, Lotte Stenfors Arnesen, Truls Leegaard, Arnfinn Sundsfjord, Pål A. Jenum Tags: Original Article Source Type: research
Issue Information
(Source: APMIS)
Source: APMIS - June 15, 2017 Category: Research Tags: Issue Information Source Type: research
