Non ‐alcoholic fatty liver disease – histological scoring systems: a large cohort single‐center, evaluation study
This study was aimed to evaluate the two histological scoring systems (NAS‐CRN, SAF) in the diagnosis of NAFLD and to assess the role of histological characteristics as injury markers in NAFLD. Retrospective histological study of liver biopsies of 1000 patients diagnosed as NAFLD, between 2010 and 2016, was conducted. Histopathologic evaluation and semiquantiative scoring based on NAS‐CRN and SAF algorithm and their correlation with serum aminotransferase and fibrosis were performed. Liver biopsies were classified according to the NAS‐CRN scoring, as NAS <3 (not NASH) in 72 (7.2%), NAS 3–4 (borderline NASH) in 3...
Source: APMIS - October 27, 2017 Category: Research Authors: Archana Rastogi, Saggere Muralikrishna Shasthry, Ayushi Agarwal, Chhagan Bihari, Priyanka Jain, Ankur Jindal, Shiv Sarin Tags: Original Article Source Type: research
Issue Information
(Source: APMIS)
Source: APMIS - October 27, 2017 Category: Research Tags: Issue Information Source Type: research
Architectural patterns of p16 immunohistochemical expression associated with cancer immunity and prognosis of head and neck squamous cell carcinoma
We evaluated the expression patterns of p16, which is used as a surrogate marker of HPV infection in head and neck squamous cell carcinoma (HNSCC), in regard to their biological and prognostic implications. p16 expression patterns and infiltrated immune cells were analyzed through immunohistochemistry of p16, CD3, CD8, PD‐1, FOXP3, and CD163 on surgically resected HNSCCs (n = 393). Patterns of p16 immunoexpression were defined as STRONG (strong, diffuse expression in cytoplasm, and nucleus in >70% of tumor cells), MARGINAL (expression restricted to tumor margins), MOSAIC (ragged, discontinued expression), NUCLEAR (exp...
Source: APMIS - October 4, 2017 Category: Research Authors: Hyang Joo Ryu, Eun Kyung Kim, Su Jin Heo, Byoung Chul Cho, Hye Ryun Kim, Sun Och Yoon Tags: Original Article Source Type: research
M2 polarization of monocytes in ankylosing spondylitis and relationship with inflammation and structural damage
The aim of this study was to evaluate the polarization of peripheral blood monocytes in the patients with ankylosing spondylitis (AS) and to determine the correlations between monocyte polarization and inflammation and structural damage. A total of 120 AS patients, 50 rheumatoid arthritis (RA) patients and 100 healthy controls were enrolled in the study. M1 (CD68+CD192+) and M2 (CX3CR1+CD163+) monocytes were characterized by flow cytometry. Demographic, clinical, radiographic and laboratory data were collected and analyzed. A large increase in M2 (CX3CR1+CD163+) monocytes was observed in AS, and M2/M1 ratio was 7.18 ± 6...
Source: APMIS - October 3, 2017 Category: Research Authors: Jinzhu Zhao, Wei Yuan, Chunsheng Tao, Peifeng Sun, Zaixing Yang, Weidong Xu Tags: Original Article Source Type: research
Dominant high expression of wild ‐type HSP110 defines a poor prognostic subgroup of colorectal carcinomas with microsatellite instability: a whole‐section immunohistochemical analysis
In conclusion, the dominant intensity score‐based assessment of HSP110wt IHC can be a simple and useful method for the prognostic stratification of MSI‐H CRCs. It is expected that HSP110wt IHC may be used to identify a subgroup of MSI‐H CRCs with poor prognosis and/or candidates for further treatment, such as immunotherapy using immune checkpoint inhibitors in MSI‐H CRCs. (Source: APMIS)
Source: APMIS - October 3, 2017 Category: Research Authors: Hyeon Jeong Oh, Jung Ho Kim, Tae Hun Lee, Hye Eun Park, Jeong Mo Bae, Hye Seung Lee, Gyeong Hoon Kang Tags: Original Article Source Type: research
Type of vascular invasion in association with progress of endometrial cancer
Vascular invasion (VI) is a well‐established marker for lymph node metastasis and outcome in endometrial cancer. Our study explored whether specific types of VI, defined as lymphatic (LVI) or blood vessel invasion (BVI), predict pattern of metastasis. From a prospectively collected cohort, we conducted a case–control study by selecting three groups of endometrial cancer patients (n = 183): 52 with positive lymph nodes at primary surgery, 33 with negative nodes at primary surgery and later recurrence and death from disease, and 98 with negative nodes and no recurrence. All patients underwent hysterectomy with lymphade...
Source: APMIS - October 1, 2017 Category: Research Authors: Nicole C.M. Visser, Henrica M.J. Werner, Camilla Krakstad, Karen K. Mauland, Jone Trovik, Leon F.A.G. Massuger, Iris D. Nagtegaal, Johanna M.A. Pijnenborg, Helga B. Salvesen, Johan Bulten, Ingunn M. Stefansson Tags: Original Article Source Type: research
Cancer/testis antigen SPATA19 is frequently expressed in benign prostatic hyperplasia and prostate cancer
In conclusion, we showed that SPATA19 protein was frequently expressed in both BPH and PCa glands, and this warrants future investigations on its pathogenic roles in the disease. (Source: APMIS)
Source: APMIS - October 1, 2017 Category: Research Authors: Kah Keng Wong, Faezahtul Arbaeyah Hussain, Suet Kee Loo, Jos é I. López Tags: Original Article Source Type: research
Epidemiological characterization of a nosocomial outbreak of extended spectrum β‐lactamase Escherichia coli ST‐131 confirms the clinical value of core genome multilocus sequence typing
Enhanced precision of epidemiological typing in clinically suspected nosocomial outbreaks is crucial. Our aim was to investigate whether single nucleotide polymorphism (SNP) analysis and core genome (cg) multilocus sequence typing (MLST) of whole genome sequencing (WGS) data would more reliably identify a nosocomial outbreak, compared to earlier molecular typing methods. Sixteen isolates from a nosocomial outbreak of ESBL E. coli ST‐131 in southeastern Sweden and three control strains were subjected to WGS. Sequences were explored by SNP analysis and cgMLST. cgMLST clearly differentiated between the outbreak isolates an...
Source: APMIS - September 28, 2017 Category: Research Authors: Hanna Woksepp, Anna Ryberg, Linda Berglind, Thomas Sch ön, Jan Söderman Tags: Original Article Source Type: research
Expression of CD markers' in immune thrombocytopenic purpura: prognostic approaches
Immune Thrombocytopenic Purpura (ITP) is a common autoimmune bleeding disorder characterized by a reduction in peripheral blood platelet counts. In this disease, autoantibodies (Auto‐Abs) are produced against platelet GPIIb/GPIIIa by B cells, which require interaction with T cells. In this review, the importance of B and T lymphocytes in ITP prognosis has been studied. Relevant literature was identified by a PubMed search (1990–2016) of English‐language papers using the terms B and T lymphocyte, platelet, CD markers and immune thrombocytopenic purpura. T and B lymphocytes are the main immune cells in the body. Defect...
Source: APMIS - September 28, 2017 Category: Research Authors: Masumeh Maleki Behzad, Ali Amin Asnafi, Kaveh Jaseb, Mohammad Ali Jalali Far, Najmaldin Saki Tags: Review Article Source Type: research
Occurrence of five distinct red cell alloantibodies in a renal transplant recipient: Diagnostic and therapeutic implications of minor histocompatibility antigens (Kidd and Duffy) for renal allograft outcome
(Source: APMIS)
Source: APMIS - September 28, 2017 Category: Research Authors: Rajeswari Subramaniyan Tags: Letter to the Editor Source Type: research
Metastatic mature teratoma to the neck with respiratory ‐type epithelium: a case requiring evidence of chromosome 12p overrepresentation to differentiate malignant and benign diagnoses
(Source: APMIS)
Source: APMIS - September 28, 2017 Category: Research Authors: Miroslav Sekulic, Michelle Dolan, Paari Murugan, Faqian Li Tags: Letter to the Editor Source Type: research
Angiogenesis in Schistosoma haematobium ‐associated urinary bladder cancer
Schistosoma haematobium, a parasitic flatworm that infects more than 100 million people, mostly in the developing world, is the causative agent of urogenital schistosomiasis, and is associated with a high incidence of squamous cell carcinoma (SCC) of the bladder. During infection, eggs are deposited in the bladder causing an intense inflammatory reaction. Angiogenesis is defined as the formation of new blood vessels from preexisting ones and is recognized as a key event in cell proliferation and carcinogenesis and spread of malignant lesions. A growing amount of evidence points to angiogenesis playing a key role in schisto...
Source: APMIS - September 28, 2017 Category: Research Authors: Anderson Dematei, R úben Fernandes, Raquel Soares, Helena Alves, Joachim Richter, Monica C. Botelho Tags: Review Article Source Type: research
Strong antimicrobial activity of xanthohumol and other derivatives from hops (Humulus lupulus L.) on gut anaerobic bacteria
Anaerobic bacteria, such as Bacteroides fragilis or Clostridium perfringens, are part of indigenous human flora. However, Clostridium difficile represents also an important causative agent of nosocomial infectious antibiotic‐associated diarrhoea. Treatment of C. difficile infection is problematic, making it imperative to search for new compounds with antimicrobial properties. Hops (Humulus lupulus L.) contain substances with antibacterial properties. We tested antimicrobial activity of purified hop constituents humulone, lupulone and xanthohumol against anaerobic bacteria. The antimicrobial activity was established agai...
Source: APMIS - September 28, 2017 Category: Research Authors: Pavel Cermak, Jana Olsovska, Alexandr Mikyska, Martin Dusek, Zuzana Kadleckova, Jiri Vanicek, Otakar Nyc, Karel Sigler, Vanda Bostikova, Pavel Bostik Tags: Original Article Source Type: research
The 16S rRNA gene ‐based PCR method used for the detection of segmented filamentous bacteria in the intestinal microbiota generates false‐positive results
(Source: APMIS)
Source: APMIS - September 20, 2017 Category: Research Authors: Forough L. Nowrouzian, Liselott Svensson Stadler, Ingegerd Adlerberth, Agnes E. Wold Tags: Letter to the Editor Source Type: research
Hypogammaglobulinemia in children: a warning sign to look deeply?
This study investigated phenotypic and functional characteristics of lymphocytes in children with common variable immunodeficiency (CVID) and unclassified hypogammaglobulinemia (UH), as well as B‐cell subsets in non‐consanguineous parents. Blood samples of 30 children, CVID (n = 9), UH (n = 9), healthy donors HD (n = 12), and 19 adults (parents and controls) were labeled by a combination of surface markers to identify CD4, CD8 T‐cell and B‐cell subpopulations. T‐cell cytokine production in children was analyzed in vitro after stimulation with phytohemagglutinin (PHA) and tetanus toxoid. We observed low percentage...
Source: APMIS - September 20, 2017 Category: Research Authors: Karina Mescouto Melo, Maria Isabel Moraes ‐Pinto, Luís E. C. Andrade, Reinaldo Salomão, Milena K. C. Brunialti, Vanessa S. Ferreira, Beatriz T. Costa‐Carvalho Tags: Original Article Source Type: research
