Blood donor Borrelia burgdorferi sensu lato seroprevalence and history of tick bites at a northern limit of the vector distribution
In order to study the antibody seroprevalence of the causal agent of Lyme borreliosis, Borrelia burgdorferi sensu lato (s.l.), and the history of tick bites at a geographical distribution limit of Ixodes ricinus, we compared healthy blood donors in geographically extreme regions: the borreliosis‐endemic Vestfold County (59°N) and the region of northern Norway. Blood samples were screened using IgG/VlsE ELISA, and positive/borderline samples were confirmed using C6 ELISA and immunoblot assays. Also, donors completed a questionnaire consisting of several items including the places they have lived, and whether they owned a...
Source: APMIS - June 14, 2017 Category: Research Authors: Dag Hvidsten, Liisa Mortensen, Bj ørn Straume, Mirjana Grujic Arsenovic, Anne‐Berit Pedersen, Gro Lyngås, Jeremy S. Gray, Nils Grude Tags: Original Article Source Type: research
A DNA hypermethylation profile reveals new potential biomarkers for the evaluation of prognosis in urothelial bladder cancer
DNA hypermethylation has emerged as a molecular biomarker for the evaluation of cancer diagnosis and prognosis. We define a methylation signature of bladder cancer and evaluate whether this profile assesses prognosis of patients. Genome‐wide methylation analysis was performed on 70 tumor and 10 normal bladder samples. Hypermethylation status of 1505 CpGs present in the promoter region of 807 genes was studied. Thirty‐three genes were significantly hypermethylated in ≥10% of the tumors. Three clusters of patients were characterized by their DNA methylation profile, one at higher risk of dead of disease (p = 0.0012). A...
Source: APMIS - June 6, 2017 Category: Research Authors: Jose I. L ópez, Javier C. Angulo, Ana Martín, Manuel Sánchez‐Chapado, Ana González‐Corpas, Begoña Colás, Santiago Ropero Tags: Original Article Source Type: research
Epithelial –mesenchymal transition markers in malignant ovarian germ cell tumors
In conclusion, based on this limited series, EMT‐associated markers are not associated with clinical parameters in MOGCT, in contrast to ovarian carcinoma. EMT‐related proteins are differentially expressed among various MOGCT subtypes, suggesting differences in biological characteristics associated with invasion and metastasis. (Source: APMIS)
Source: APMIS - June 6, 2017 Category: Research Authors: Olesya Solheim, Mette F ørsund, Claes G. Tropé, Sigrid Marie Kraggerud, Jahn M. Nesland, Ben Davidson Tags: Original Article Source Type: research
Inadequate adherence to Swedish guidelines for uncomplicated lower urinary tract infections among adults in general practice
In conclusion, the adherence to national guidelines/recommendations was inadequate. To reduce the selection of multiresistant bacteria, an improvement of the use of diagnostic criteria/tools and antibiotic drugs in primary care is necessary. (Source: APMIS)
Source: APMIS - June 6, 2017 Category: Research Authors: Heidi Lindb äck, Johan Lindbäck, Åsa Melhus Tags: Original Article Source Type: research
TFE3 ‐rearranged hepatic epithelioid hemangioendothelioma—a case report with immunohistochemical and molecular study
We present the first case of TFE3‐rearranged hepatic epithelioid hemangioendothelioma in a 39‐year‐old Taiwanese woman. Computed tomography scan revealed multifocal, ill‐defined nodules involving both hepatic lobes. She then underwent deceased donor liver transplantation. Histologically, the tumors in the liver explant showed a biphasic growth pattern. One component was composed of dilated and well‐formed blood vessels lined by epithelioid cells with abundant eosinophilic cytoplasm, mimicking an alveolar pattern, whereas the other component was composed of cords and single cells, featuring intracytoplasmic vacuol...
Source: APMIS - June 6, 2017 Category: Research Authors: Fang ‐Ying Kuo, Hsuan‐Ying Huang, Chao‐Long Chen, Hock‐Liew Eng, Chao‐Cheng Huang Tags: Case Report Source Type: research
Trichomonas vaginalis is very rare among women with vaginal discharge in Podlaskie province, Poland
Trichomonas vaginalis is the most common curable sexually transmitted pathogen globally. However, in the European Union (EU), trichomoniasis appears to be a rare condition. The aim of this study was to examine the prevalence of T. vaginalis among females attending an STI centre in Bialystok, Poland, using the highly sensitive and specific APTIMA T. vaginalis assay. Consecutive females, referred by gynaecologists mainly because of abnormal vaginal discharge, were diagnosed using wet mount microscopy, culture and APTIMA T. vaginalis assay. Among 272 women studied, 82% were pre‐ and 18% postmenopausal. The average age was 3...
Source: APMIS - June 1, 2017 Category: Research Authors: Agnieszka Beata Serwin, Violetta Bulhak ‐Koziol, Marianna Sokolowska, Daniel Golparian, Magnus Unemo Tags: Original Article Source Type: research
Use of the optical disector in canine mammary simple and complex carcinomas
Grading of canine mammary carcinomas (CMC) is associated to subjective assessments made by the pathologists. Due to its unbiased nature, stereology can be used to objectively quantify morphological parameters associated with grading and malignancy. However, the use of stereology in CMC has not been fully disclosed. The nuclear numerical density [NV (nuclei, tumor)] is a cellularity‐associated parameter that can be estimated by the optical disector. Herein, it was estimated in 44 CMC and its association with clinicopathologic factors – such as tumor size, histological subtype and grade, vascular/lymph node invasion, nuc...
Source: APMIS - June 1, 2017 Category: Research Authors: Marta Santos, Patr ícia Dias‐Pereira, Carla Correia‐Gomes, Ricardo Marcos, Augusto Matos, Eduardo Rocha, Carlos Lopes Tags: Original Article Source Type: research
Circulation and diagnostics of Puumala virus in Norway: nephropatia epidemica incidence and rodent population dynamics
Hantaviruses pose a public health concern worldwide causing haemorrhagic fever with renal syndrome (HFRS) and hantavirus pulmonary syndrome (HPS). Puumala virus (PUUV) is the most prevalent hantavirus in Central and Northern Europe, and causes a mild form of HFRS, also known as nephropathia epidemica (NE). In nature, the main host of PUUV is the bank vole (Myodes glareolus), and transmission to humans occurs through inhalation of aerosols from rodent excreta. Nephropathia epidemica is particularly prevalent in Nordic countries, however, few studies of PUUV have been performed in Norway. The aim of this study was to analyse...
Source: APMIS - June 1, 2017 Category: Research Authors: Natacha Milhano, Lars Korslund, Magnus Evander, Clas Ahlm, Kirsti Vainio, Susanne G. Dudman, Åshild Andreassen Tags: Original Article Source Type: research
Confirmed identification and toxin profiling of Campylobacter jejuni using a thermostabilized multiplex PCR formulation
Cytolethal distending toxin (CDT) producing Campylobacter jejuni species are one of the leading causes of human gastroenteritis worldwide. The main intent of the study was to develop a multiplex PCR assay for the confirmed identification and toxin profiling of C. jejuni. The genes targeted were rpo B as genus specific, hip O for species; cdt A, cdt B, cdt C encoding respective subunit proteins of CDT with Internal Amplification Control (IAC). To enhance its application as a pre‐mixed ready‐to‐use format, the master mix of developed mPCR was dried by lyophilization and stability was assessed. Thermostabilized reagents...
Source: APMIS - June 1, 2017 Category: Research Authors: Nitya Ramachandran, Shylaja Ramlal, Harsh Vardhan Batra Tags: Original Article Source Type: research
Prognostic value of p21 ‐activated kinase 4 in resected pancreatic cancer
In conclusion, PAK4 of the markers tested in this study may be a potential prognostic biomarker for pancreatic adenocarcinomas. (Source: APMIS)
Source: APMIS - May 30, 2017 Category: Research Authors: Sehhoon Park, Jin Won Kim, Haeryoung Kim, Ji ‐Won Kim, Yu Jung Kim, Keun‐Wook Lee, Jee Hyun Kim, Jai Hwan Kim, Jin‐Hyeok Hwang, Young Rok Choi, Jai Young Cho, Yoo‐Seok Yoon, Ho‐Seong Han Tags: Original Article Source Type: research
Issue Information
(Source: APMIS)
Source: APMIS - May 30, 2017 Category: Research Tags: Issue Information Source Type: research
Polymorphism of TLR5 rs5744174 is associated with disease progression in Chinese patients with chronic HBV infection
Toll‐like receptors (TLRs) play a crucial role in innate and adaptive immunity, protecting the host from viral pathogens. Studies have implicated that TLR5 is associated with various diseases such as autoimmune and inflammation related diseases. However, little is known about the relationship between TLR5 and hepatitis B virus (HBV) infection. We studied the effect of TLR5 gene polymorphisms on susceptibility to and disease progression of chronic hepatitis B (CHB) infection in Chinese. Blood samples were taken from 636 patients with CHB, HBV‐related liver cirrhosis (LC) or hepatocellular carcinoma (HCC) and 273 control...
Source: APMIS - May 25, 2017 Category: Research Authors: Lina Cao, Tong Zhang, Junping Zhu, Aixin Li, Kai Zheng, Nan Zhang, Bin Su, Wei Xia, Hao Wu, Ning Li, Qiushui He Tags: Original Article Source Type: research
NUT carcinoma presenting in the palate – a case report
This article presents a case of a 60‐year‐old woman diagnosed with NC presenting as a fast growing primary tumour in the right palate. Further evaluation revealed a tumour mass in the lungs and widespread metastases. A review of the literature did not reveal earlier cases presenting in the palate. In order to improve early diagnosis it is suggested to perform immunohistochemical testing for NUT in all poorly differentiated carcinomas without glandular differentiation arising in the chest, and head and neck (Clin Cancer Res, 18, 2012, 5773). (Source: APMIS)
Source: APMIS - May 25, 2017 Category: Research Authors: Libana Raffoul Bjornstrup, Jesper Reibel, Katalin Kiss, Morten Schioedt Tags: Case Report Source Type: research
A unique case of bilateral ovarian splenosis and review of the literature
We report the first definitive bilateral ovarian case in a 65‐year‐old woman who underwent splenectomy following a motor vehicle accident 44 years prior to presentation. We review the literature and discuss the main differential diagnoses. Gross examination revealed a 1‐cm well‐circumscribed dark nodule on the surface of each ovary. Paraffin‐embedded, formalin‐fixed blocks were sectioned and stained with hematoxylin–eosin and immunostains (CK5/6, Calretinin, WT1, Vimentin). The histological presence of both red and white splenic pulp, delimitation from ovarian tissue and ovarian origin of blood supply, as we...
Source: APMIS - May 25, 2017 Category: Research Authors: Maria Giulia Disanto, Francesca Mercalli, Andrea Palicelli, Alberto Arnulfo, Renzo Boldorini Tags: Case Report Source Type: research
Expression and prognostic significance of programmed death protein 1 and programmed death ligand ‐1, and cytotoxic T lymphocyte‐associated molecule‐4 in hepatocellular carcinoma
In this study, we assessed the correlation between clinicopathologic factors with programmed cell death protein 1 (PD‐1) and programmed cell death ligand‐1 (PD‐L1), and cytotoxic T lymphocyte‐associated molecule‐4 (CTLA‐4) expressions. Furthermore, we analyzed the prognostic significance of these proteins in a subgroup of patients. We retrospectively evaluated the PD‐1, PD‐L1, and CTLA‐4 expressions in 294 HCC tissue microarray samples using immunohistochemistry. PD‐1 and PD‐L1 expressions were significant related to high CD8+ tumor‐infiltrating lymphocytes (TILs) (r = 0.664, p < 0.001 and r = 0....
Source: APMIS - May 11, 2017 Category: Research Authors: Hyeyoon Chang, Wonkyung Jung, Aeree Kim, Han Kyeom Kim, Wan Bae Kim, Ji Hoon Kim, Baek ‐hui Kim Tags: Original Article Source Type: research
