Failure of transplantation tolerance induction by autologous regulatory T cells in the pig‐to‐non‐human primate islet xenotransplantation model
ConclusionsTaken together, autologous Tregs infused at the peri‐transplantation period failed to induce transplantation tolerance in pig‐to‐NHP islet xenotransplantation setting. (Source: Xenotransplantation)
Source: Xenotransplantation - July 6, 2016 Category: Transplant Surgery Authors: Jun‐Seop Shin, Byoung‐Hoon Min, Jong‐Min Kim, Jung‐Sik Kim, Il Hee Yoon, Hyun Je Kim, Yong‐Hee Kim, Jae Yool Jang, Hee Jung Kang, Dong‐Gyun Lim, Jongwon Ha, Sang‐Joon Kim, Chung‐Gyu Park Tags: Original Article Source Type: research
Human antibody reactivity against xenogeneic N ‐glycolylneuraminic acid and galactose‐α‐1,3‐galactose antigen
ConclusionsWe showed that IgG‐mediated CDC was detected in Neu5Gc‐overexpressed HEK293 cells incubated with human sera; however, this antibody reactivity to Neu5Gc was highly variable among individuals. Our results suggest that additional modifications to the CMAH gene should be considered for widespread use of pig organs for human transplants. (Source: Xenotransplantation)
Source: Xenotransplantation - July 3, 2016 Category: Transplant Surgery Authors: Sunghoon Hurh, Bohae Kang, Inho Choi, Bumrae Cho, Eun Mi Lee, Hwajung Kim, Young June Kim, Yun Shin Chung, Jong Cheol Jeong, Jong ‐Ik Hwang, Jae Young Kim, Byeong Chun Lee, Charles D. Surh, Jaeseok Yang, Curie Ahn Tags: Original Article Source Type: research
Thyroid hormone: relevance to xenotransplantation
ConclusionsFollowing operative procedures, there is a dramatic fall in serum fT3 levels. The persistent low level of fT3 after pig heart and liver xenoTx may be associated with a continuing inflammatory state. We suggest that consideration should be given to the replacement of T3 therapy to maintain normal fT3 levels, particularly in nonhuman primates undergoing orthotopic pig heart or liver xenoTx. (Source: Xenotransplantation)
Source: Xenotransplantation - July 3, 2016 Category: Transplant Surgery Authors: Hayato Iwase, Burcin Ekser, Hidetaka Hara, Mohamed B. Ezzelarab, Cassandra Long, Angus W. Thomson, David Ayares, David K. C. Cooper Tags: Original Article Source Type: research
Extended microbiological characterization of G öttingen minipigs: porcine cytomegalovirus and other viruses
ConclusionUsing highly sensitive methods, PCMV, HEV, and PLHV were found in some GöMP, suggesting that these viruses may be introduced through the placenta. The results show that highly sensitive methods are required to characterize pigs to be used for xenotransplantation to prevent virus transmission. (Source: Xenotransplantation)
Source: Xenotransplantation - June 30, 2016 Category: Transplant Surgery Authors: Vladimir A. Morozov, Elena Plotzki, Avi Rotem, Uriel Barkai, Joachim Denner Tags: Original Article Source Type: research
Psychosocial challenges of xenotransplantation: the need for a multidisciplinary, religious, and cultural dialogue
(Source: Xenotransplantation)
Source: Xenotransplantation - June 30, 2016 Category: Transplant Surgery Authors: Wayne Paris, Kyeonghee Jang, Leah Colsch, Ali Prus, Ruth Bargainer, Bakr Nour, David K.C. Cooper Tags: Letter to the Editor Source Type: research
Characterization of immunogenic Neu5Gc in bioprosthetic heart valves
ConclusionsWe conclusively demonstrated Neu5Gc expression in native cardiac tissues, as well as in six commercial BHV. These Neu5Gc xeno‐antigens were recognized by human anti‐Neu5Gc IgG, supporting their immunogenicity. Altogether, these findings suggest BHV‐Neu5Gc/anti‐Neu5Gc may play a role in valve deterioration warranting further investigation. (Source: Xenotransplantation)
Source: Xenotransplantation - June 30, 2016 Category: Transplant Surgery Authors: Eliran Moshe Reuven, Shani Leviatan Ben ‐Arye, Tal Marshanski, Michael E. Breimer, Hai Yu, Imen Fellah‐Hebia, Jean‐Christian Roussel, Cristina Costa, Manuel Galiñanes, Rafael Mañez, Thierry Le Tourneau, Jean‐Paul Soulillou, Emanuele Cozzi, Xi Ch Tags: Original Article Source Type: research
Comparison of methods for isolating primary hepatocytes from mini pigs
Abstract
Successful porcine hepatocyte isolation is crucial for the development of bioartificial liver devices and hepatocyte transplantation. Serva collagenase NB grades are formulated collagenases that are suitable for various tissue isolation applications. N‐acetylcysteine (NAC) can improve the viability of human hepatocytes. The aim of this study was to compare the effectiveness of two collagenases and effect of NAC on hepatocyte isolation from porcine liver tissue. Porcine hepatocytes were isolated using the perfusion method from Bama mini pigs assigned to the Serva NB 4 group (n=6), the Serva NB 8 group (n=6), or t...
Source: Xenotransplantation - June 30, 2016 Category: Transplant Surgery Authors: Yi Li, Yujia Wang, Qiong Wu, Li Li, Yujun Shi, Hong Bu, Ji Bao Tags: Brief Communication Source Type: research
Corrigendum
(Source: Xenotransplantation)
Source: Xenotransplantation - June 7, 2016 Category: Transplant Surgery Tags: Corrigendum Source Type: research
Pig ‐to‐nonhuman primate solid organ xenografting: recent achievements on the road to first‐in‐man explorations
(Source: Xenotransplantation)
Source: Xenotransplantation - June 5, 2016 Category: Transplant Surgery Authors: Henk ‐Jan Schuurman Tags: Commentary Source Type: research
Xenotransplantation literature update, March –April 2016
(Source: Xenotransplantation)
Source: Xenotransplantation - May 29, 2016 Category: Transplant Surgery Authors: Christopher Burlak Tags: Literature Update Source Type: research
As2O3 combined with leflunomide prolongs heart xenograft survival via suppressing the response of Th1, Th2, and B cells in a rat model
In this study, we systematically investigated the immunosuppressive effect and mechanism of action of As2O3 and leflunomide using a hamster‐to‐rat heart xenotransplantation model. We initially examined heart xenograft survival following As2O3 and leflunomide treatment alone or combined treatment. We found that treatment with As2O3 combined with leflunomide can significantly prolong the survival of heart xenograft by inhibiting Th1 and Th2 differentiation and reducing the production of IgG and IgM. Interestingly, As2O3 and leflunomide showed low toxicity to the organs of the recipient. Taken together, these observations...
Source: Xenotransplantation - May 17, 2016 Category: Transplant Surgery Authors: Zhi‐Xing Jiao, Yun Leng, Jun‐Jie Xia, Hai‐Qiao Wu, Ning Jin, Jia‐Zhao Fu, Lian‐Na Cheng, Jin‐Hua Wang, Shao‐Bin Ni, Zhong‐Quan Qi Tags: Original Article Source Type: research
Biophysico ‐functional compatibility of Seoul National University (SNU) miniature pig cornea as xenocorneal graft for the use of human clinical trial
ConclusionsIn conclusion, SNU pig cornea is feasible for xenocorneal transplantation using the same preservation protocol as human with respect to biophysical and functional properties and can be stored for up to 7 days for transplantation in human clinical trials. An age limitation of donor pigs may be required for qualified corneal products to be used in human trials. (Source: Xenotransplantation)
Source: Xenotransplantation - May 10, 2016 Category: Transplant Surgery Authors: Dong Hyun Kim, Jaeyoung Kim, Hyun Jeong Jeong, Hyun Ju Lee, Mee Kum Kim, Won Ryang Wee Tags: Original Article Source Type: research
Human antibody recognition of xenogeneic antigens (NeuGc and Gal) on porcine heart valves: could genetically modified pig heart valves reduce structural valve deterioration?
ConclusionCompared to WT pigs, GTKO/CD46/NeuGcKO pigs would be preferable sources of GBHVs, because the absence of Gal/NeuGc expression reduces human antibody binding. (Source: Xenotransplantation)
Source: Xenotransplantation - April 30, 2016 Category: Transplant Surgery Authors: Whayoung Lee, Cassandra Long, Jagdeece Ramsoondar, David Ayares, David K. C. Cooper, Rizwan A. Manji, Hidetaka Hara Tags: Original Article Source Type: research
Diabetes induction by total pancreatectomy in minipigs with simultaneous splenectomy: a feasible approach for advanced diabetes research
ConclusionsSurgical pancreatectomy in combination with splenectomy to facilitate access to the pancreas is a feasible model for efficient diabetes induction in minipigs. The procedure itself and postoperative animal care could be performed without complications in this exploratory study. Nevertheless, this approach requires well‐equipped infrastructure, experienced and skilled surgeons and anesthesiologists and dedicated animal care takers. The impact of total pancreatectomy in combination with splenectomy on the digestive and immune system must be considered in the design and definition of end points of experimental dia...
Source: Xenotransplantation - April 30, 2016 Category: Transplant Surgery Authors: Sophie Heinke, Barbara Ludwig, Undine Schubert, Janine Schmid, Thomas Kiss, Anja Steffen, Stefan Bornstein, Stefan Ludwig Tags: Original Article Source Type: research
Porcine antigen ‐specific IFN‐γ ELISpot as a potentially valuable tool for monitoring cellular immune responses in pig‐to‐non‐human primate islet xenotransplantation
ConclusionUse of PBMCs in a porcine antigen‐specific IFN‐γ ELISpot assay is a reliable method for monitoring T‐cell‐mediated rejection in pig‐to‐NHP islet xenotransplantation. (Source: Xenotransplantation)
Source: Xenotransplantation - April 30, 2016 Category: Transplant Surgery Authors: Hyun ‐Je Kim, Il‐Hee Yoon, Byoung‐Hoon Min, Yong‐Hee Kim, Jun‐Seop Shin, Jong‐Min Kim, Jung‐Sik Kim, Hye‐Young Nam, Won‐Woo Lee, Chung‐Gyu Park Tags: Original Article Source Type: research
