The impact of serum incubation time on IgM/IgG binding to porcine aortic endothelial cells
Abstract
The results of the assay for measuring anti‐non‐Gal antibodies (which affect pig xenograft survival) in recipients are important. Serum incubation time and concentration may be important factors in the extent of antibody binding to the graft. The aim of this in vitro study was to determine the optimal incubation time and serum concentration for measuring anti‐non‐Gal antibody binding to porcine aortic endothelial cells (pAECs). Pooled human, naive, and sensitized baboon sera were incubated with wild‐type, α1,3‐galactosyltransferase gene‐knockout (GTKO), and GTKO/human CD55 pAECs. IgM/IgG binding to ...
Source: Xenotransplantation - March 1, 2017 Category: Transplant Surgery Authors: Zhongqiang Zhang, Bingsi Gao, Chengjiang Zhao, Cassandra Long, Haizhi Qi, Mohamed Ezzelarab, David KC Cooper, Hidetaka Hara Tags: BRIEF COMMUNICATION Source Type: research
Effects of encapsulated porcine islets on glucose and C ‐peptide concentrations in diabetic nude mice 6 months after intraperitoneal transplantation
ConclusionsThe peripheral blood sampling for c‐peptide, though highly informative in vascularized grafts, may not be the primary tool for monitoring the health and function of encapsulated products when transplanted into intraperitoneal cavity. Our results might explain the clinical feature of the low C‐peptide blood concentrations after successful intraperitoneal encapsulated islet transplantation. (Source: Xenotransplantation)
Source: Xenotransplantation - March 1, 2017 Category: Transplant Surgery Authors: Masuhiro Nishimura, Naho Iizuka, Yasutaka Fujita, Osamu Sawamoto, Shinichi Matsumoto Tags: ORIGINAL ARTICLE Source Type: research
Long ‐term safety from transmission of porcine endogenous retrovirus after pig‐to‐non‐human primate corneal transplantation
ConclusionsWithin the limits of this study, there is no evidence to support any risk of PERV transmission from porcine corneal tissues to NHP recipients, despite the existence of PERV‐expressing cells in porcine corneas. (Source: Xenotransplantation)
Source: Xenotransplantation - March 1, 2017 Category: Transplant Surgery Authors: Hyuk Jin Choi, Jiyeon Kim, Jae Young Kim, Hyun Ju Lee, Won Ryang Wee, Mee Kum Kim, Eung Soo Hwang Tags: ORIGINAL ARTICLE Source Type: research
Angiopoietin ‐1 and angiopoietin‐2 protect porcine iliac endothelial cells from human antibody‐mediated complement‐dependent cytotoxicity through phosphatidylinositide 3‐kinase/AKT pathway activation
In conclusion, our data suggest that human Ang‐1/Ang‐2 induces the protection of PIECs from human antibody‐mediated CDC by activating the PI3K/AKT pathway. Ang‐1/Ang‐2 is likely to protect porcine endothelial cells and may be beneficial in xenotransplantation research. (Source: Xenotransplantation)
Source: Xenotransplantation - March 1, 2017 Category: Transplant Surgery Authors: Hanchao Gao, Pengfei Chen, Ling Wei, Jia Xu, Lu Liu, Yanli Zhao, Hidetaka Hara, Dengke Pan, Zesong Li, David K.C. Cooper, Zhiming Cai, Lisha Mou Tags: BRIEF COMMUNICATION Source Type: research
Characterization of porcine endogenous retrovirus expression in neonatal and adult pig pancreatic islets
ConclusionOur study confirms the low level of PERV expression in whole pancreas in a large population of both neonatal and adult pigs (n=80). The level of PERV expression was however higher in the endocrine tissue than in the exocrine cells. There was no correlation between PERV status in donor PBMCs and islet cells, and no evidence of active replication in in vitro regardless of PERV expression in islet cells. Moreover, neonatal pig islets were found to have significantly lower PERV expression compared to adult islets. Neonatal islets have been suggested as the best choice for xenotransplantation in terms of economic and ...
Source: Xenotransplantation - March 1, 2017 Category: Transplant Surgery Authors: Nizar I. Mourad, Claire Crossan, Victoria Cruikshank, Linda Scobie, Pierre Gianello Tags: ORIGINAL ARTICLE Source Type: research
Human galectin ‐9 on the porcine cells affects the cytotoxic activity of M1‐differentiated THP‐1 cells through inducing a shift in M2‐differentiated THP‐1 cells
ConclusionsThese data suggested that hGal‐9 has a reduction in M1‐differentiated THP‐1 cell cytotoxic activity‐related acute immune rejection in pig‐to‐human xenotransplantation in addition to its role in lymphoid lineage immune cell regulation. (Source: Xenotransplantation)
Source: Xenotransplantation - March 1, 2017 Category: Transplant Surgery Authors: Sung Han Jung, Jeong Ho Hwang, Sang Eun Kim, Young Kyu Kim, Hyo Chang Park, Hoon Taek Lee Tags: ORIGINAL ARTICLE Source Type: research
Issue Information
(Source: Xenotransplantation)
Source: Xenotransplantation - February 27, 2017 Category: Transplant Surgery Tags: ISSUE INFORMATION Source Type: research
Transplantation of hepatocytes from genetically engineered pigs into baboons
ConclusionsAs a result of this disappointing experience, the following points need to be considered. (i) Were the isolated pig hepatocytes functionally viable? (ii) Are pig hepatocytes more immunogenic than pig hearts, kidneys, artery patch grafts, or islets? (iii) Does injection of pig cells (antigens) into the spleen and/or lymph nodes stimulate a greater immune response than when pig tissues are grafted at other sites? (iv) Did the presence of the recipient's intact liver prevent survival and proliferation of pig hepatocytes? (v) Is pig CD47‐primate SIRP‐α compatibility essential? In conclusion, the transplantation...
Source: Xenotransplantation - January 27, 2017 Category: Transplant Surgery Authors: Hayato Iwase, Hong Liu, Eva Schmelzer, Mohamed Ezzelarab, Martin Wijkstrom, Hidetaka Hara, Whayoung Lee, Jagjit Singh, Cassandra Long, Eric Lagasse, J örg C. Gerlach, David K. C. Cooper, Bruno Gridelli Tags: ORIGINAL ARTICLE Source Type: research
D ‐dimer level, in association with humoral responses, negatively correlates with survival of porcine islet grafts in non‐human primates with immunosuppression
ConclusionsIntravascular coagulation, in association with immune responses against xenografts, may partly contribute to loss of islet grafts in NHPs after PITx. (Source: Xenotransplantation)
Source: Xenotransplantation - January 1, 2017 Category: Transplant Surgery Authors: Hee Jung Kang, Haneulnari Lee, Eun Mi Park, Jong ‐Min Kim, Byoung‐Hoon Min, Chung‐Gyu Park Tags: ORIGINAL ARTICLE Source Type: research
Generation of chimeric minipigs by aggregating 4 ‐ to 8‐cell‐stage blastomeres from somatic cell nuclear transfer with the tracing of enhanced green fluorescent protein
ConclusionsTo our knowledge, this is the first report of porcine chimeras generated by aggregating 4‐ to 8‐cell‐stage blastomeres from SCNT. We detected chimerism only in the skin, heart, and kidneys. Collectively, these results indicate that aggregation using 4‐ to 8‐cell‐stage SCNT embryos offers a practical approach for producing chimeric minipigs. Furthermore, it also provides a potential platform for generating interspecific chimeras between pigs and non‐human primates for xenotransplantation. (Source: Xenotransplantation)
Source: Xenotransplantation - January 1, 2017 Category: Transplant Surgery Authors: Huili Ji, Chuan Long, Chong Feng, Ningning Shi, Yingdi Jiang, Guomin Zeng, Xirui Li, Jingjing Wu, Lin Lu, Shengsheng Lu, Dengke Pan Tags: Original Article Source Type: research
Immunological and physiological observations in baboons with life ‐supporting genetically engineered pig kidney grafts
ConclusionsThe combination of (i) a graft from a specific six‐gene genetically modified pig, (ii) an effective immunosuppressive regimen, and (iii) anti‐inflammatory therapy prevented immune injury, a protein‐losing nephropathy, and coagulation dysfunction for >7 months. Although the number of experiments is very limited, our impression is that expression of human endothelial protein C receptor (±CD55) in the graft is important if coagulation dysregulation is to be avoided. (Source: Xenotransplantation)
Source: Xenotransplantation - December 31, 2016 Category: Transplant Surgery Authors: Hayato Iwase, Hidetaka Hara, Mohamed Ezzelarab, Tao Li, Zhongqiang Zhang, Bingsi Gao, Hong Liu, Cassandra Long, Yi Wang, Amy Cassano, Edwin Klein, Carol Phelps, David Ayares, Abhinav Humar, Martin Wijkstrom, David K. C. Cooper Tags: ORIGINAL ARTICLE Source Type: research
Therapeutic regulation of systemic inflammation in xenograft recipients
Abstract
Inflammation is known to preclude tolerance after transplantation. We have previously shown that systemic inflammation in xenograft recipients (SIXR) precedes activation of coagulation in the absence of T cell responses. Accordingly, SIXR may amplify innate and adaptive immune responses against xenografts after pig‐to‐primate xenotransplantation, even with efficient immunosuppressive therapy. We evaluated the impact of anti‐inflammatory agents on pro‐inflammatory cytokines and chemokines in pig artery patch and heart xenograft recipients. Baboons received an artery patch (Group1, n=8) or heart (Group2, n=4...
Source: Xenotransplantation - December 31, 2016 Category: Transplant Surgery Authors: Hayato Iwase, Hong Liu, Tao Li, Zhongquiang Zhang, Bingsi Gao, Hidetaka Hara, Martin Wijkstrom, Cassandra Long, Ryan Saari, David Ayares, David K. C. Cooper, Mohamed B. Ezzelarab Tags: BRIEF COMMUNICATION Source Type: research
Testing of microencapsulated porcine hepatocytes in a new model of fulminant liver failure in baboons
ConclusionsThe results indicate that microencapsulated porcine hepatocytes provide temporary liver function support in baboons with fulminant liver failure. These data support development of this cell therapy product toward clinical trials in patients with acute liver failure. (Source: Xenotransplantation)
Source: Xenotransplantation - December 31, 2016 Category: Transplant Surgery Authors: Zurab Machaidze, Heidi Yeh, Lingling Wei, Christian Schuetz, Michele Carvello, Antonino Sgroi, Rex N. Smith, Henk ‐Jan Schuurman, David H. Sachs, Philippe Morel, James F. Markmann, Léo H. Bühler Tags: ORIGINAL ARTICLE Source Type: research
Gastrostomy tube placement for long ‐term oral drug administration in non‐human primates
ConclusionsThe complication of gastrostomy included a transient inflammation in the skin and peritonitis caused by a leakage around implantation site (one case), which could be overcome by changing suture method and tube type to interlocking box suture and Tri‐Funnel Replacement Gastrostomy tube, respectively. Despite these complications, oral drug administration using gastrostomy tube allowed us to perform accurate dosage of drug administration and to reduce the stress that both the monkey and the researcher may experience. Taken together, this study showed that gastrostomy tube placement is a better alternative to oral...
Source: Xenotransplantation - December 31, 2016 Category: Transplant Surgery Authors: Jong ‐Min Kim, Jun‐Seop Shin, Byoung‐Hoon Min, Jung‐Sik Kim, Il‐Hee Yoon, Won‐Young Jeong, Ga‐Eul Lee, Min‐Sun Kim, Ju‐Eun Kim, Chung‐Gyu Park Tags: ORIGINAL ARTICLE Source Type: research
Issue Information
(Source: Xenotransplantation)
Source: Xenotransplantation - December 16, 2016 Category: Transplant Surgery Tags: Issue Information Source Type: research
