Inhibition of B ‐cell activation and antibody production by triggering inhibitory signals via the PD‐1/PD‐ligand pathway
ConclusionsEnhancing inhibitory signals may be part of future protocols to better control humoral immunity to allo‐ and xenografts. (Source: Xenotransplantation)
Source: Xenotransplantation - September 9, 2016 Category: Transplant Surgery Authors: Anna Buermann, Dorothee R ömermann, Wiebke Baars, Joachim Hundrieser, Jürgen Klempnauer, Reinhard Schwinzer Tags: Original Article Source Type: research
Porcine endothelium induces DNA –histone complex formation in human whole blood: a harmful effect of histone on coagulation and endothelial activation
ConclusionsDNA–histone complex actively generated upon xenotransplantation is a novel target to inhibit coagulation and endothelial activation. To prevent tissue factor and adhesion molecule expression, a strategy to block soluble histone may be required in xenotransplantation. (Source: Xenotransplantation)
Source: Xenotransplantation - September 9, 2016 Category: Transplant Surgery Authors: Hyun Ju Yoo, Ji ‐Eun Kim, Ja Yoon Gu, Sae Bom Lee, Hyun Joo Lee, Ho Young Hwang, Yoohwa Hwang, Young Tae Kim, Hyun Kyung Kim Tags: Original Article Source Type: research
Efficient production of biallelic GGTA1 knockout pigs by cytoplasmic microinjection of CRISPR/Cas9 into zygotes
ConclusionsThese results demonstrate the efficacy of CRISPR/Cas to generate genetic modifications in pigs by simplified technology, such as intracytoplasmic microinjection into zygotes, which would significantly facilitate the production of genetically modified pigs suitable for xenotransplantation. Importantly, this simplified injection protocol avoids the penetration of the vulnerable pronuclear membrane, and is thus compatible with higher survival rates of microinjected embryos, which in turn facilitates production of genetically modified piglets. (Source: Xenotransplantation)
Source: Xenotransplantation - September 8, 2016 Category: Transplant Surgery Authors: Bjoern Petersen, Antje Frenzel, Andrea Lucas ‐Hahn, Doris Herrmann, Petra Hassel, Sabine Klein, Maren Ziegler, Klaus‐Gerd Hadeler, Heiner Niemann Tags: Original Article Source Type: research
Xenotransplantation literature update, September –October 2016
(Source: Xenotransplantation)
Source: Xenotransplantation - August 31, 2016 Category: Transplant Surgery Authors: AnAn Hua, Magie Steinhoff, Christopher Burlak Tags: Literature Update Source Type: research
Microbiological safety of clinical xenotransplantation products: monitoring strategies and regulatory aspects. A commentary
(Source: Xenotransplantation)
Source: Xenotransplantation - August 31, 2016 Category: Transplant Surgery Authors: Henk ‐Jan Schuurman Tags: Commentary Source Type: research
Human anti ‐pig NK cell and CD8+ T‐cell responses in the presence of regulatory dendritic cells
ConclusionBoth Rapa‐DC and IL‐10‐DC controlled human anti‐porcine NK cell responses, in particular IFNγ production, whereas IL‐10‐DC presented stronger regulatory properties of anti‐porcine CTL responses. These in vitro findings indicate that regulatory DC could be a useful tool to promote xenograft tolerance in vivo. (Source: Xenotransplantation)
Source: Xenotransplantation - August 31, 2016 Category: Transplant Surgery Authors: Natacha Madelon, Gisella L. Puga Yung, J örg D. Seebach Tags: Original Article Source Type: research
The effect of hypoxia on free and encapsulated adult porcine islets —an in vitro study
ConclusionsHypoxia (2%‐5% oxygen or pO2: 15.2‐38.0 mm Hg) affects the viability, metabolic activity, and insulin secretion of both free and encapsulated APIs over a six‐day culture period. Encapsulation augments islet integrity under hypoxia, but it does not prevent loss of viability, metabolic activity, or insulin secretion. (Source: Xenotransplantation)
Source: Xenotransplantation - August 31, 2016 Category: Transplant Surgery Authors: Sudhakar Muthyala, Susan Safley, Kereen Gordan, Graham Barber, Collin Weber, Athanassios Sambanis Tags: Original Article Source Type: research
Induction, management, and complications of streptozotocin ‐induced diabetes mellitus in rhesus monkeys
ConclusionsStreptozotocin was effective for inducing DM in rhesus monkeys, but various adverse effects such as pancreatitis, liver toxicity or death were observed. Therefore, careful and suitable medical managements should be implemented to eliminate the risks of mortality and severe adverse effects. (Source: Xenotransplantation)
Source: Xenotransplantation - August 31, 2016 Category: Transplant Surgery Authors: Jong ‐Min Kim, Jun‐Seop Shin, Byoung‐Hoon Min, Hyun‐Je Kim, Jung‐Sik Kim, Il‐Hee Yoon, Won‐Young Jeong, Ga‐Eul Lee, Min‐Sun Kim, Ju‐Eun Kim, Sang‐Man Jin, Chung‐Gyu Park Tags: Original Article Source Type: research
Introducing the Wiley Transplant Peer Review Network
(Source: Xenotransplantation)
Source: Xenotransplantation - August 16, 2016 Category: Transplant Surgery Tags: Editorial Source Type: research
Issue Information
(Source: Xenotransplantation)
Source: Xenotransplantation - August 16, 2016 Category: Transplant Surgery Tags: Issue Information Source Type: research
Molecular immunology profiles of monkeys following xenografting with the islets and heart of α‐1,3‐galactosyltransferase knockout pigs
In this study, we evaluated the expression of a comprehensive set of immune system‐related genes (89 genes, including five housekeeping genes) in the blood of cynomolgus monkeys (~5 yr old) used as graft recipients, before and after the xenografting of the islets and heart from single and double α‐1,3‐galactosyltransferase (GalT) knockout (KO) pigs (<6 weeks old). The immunosuppressive regimen included administration of cobra venom factor, anti‐thymocyte globulin, rituximab, and anti‐CD154 monoclonal antibodies to recipients before and after grafting. Islets were xenografted into the portal vein in type 1 dia...
Source: Xenotransplantation - August 10, 2016 Category: Transplant Surgery Authors: Sun A Ock, Jungkyu Lee, Keon Bong Oh, Seongsoo Hwang, Ik Jin Yun, Curie Ahn, Hyun Keun Chee, Hwajung Kim, Jae Berm Park, Sung Joo Kim, Youngim Kim, Gi ‐Sun Im, EungWoo Park Tags: Brief Communication Source Type: research
Islet ‐derived damage‐associated molecular pattern molecule contributes to immune responses following microencapsulated neonatal porcine islet xenotransplantation in mice
ConclusionsIn conclusion, early inflammatory reactions might be therapeutic targets for the prolongation of microencapsulated NPIs graft survival. Thus, treatment‐targeting inflammation might improve the efficiency of clinical microencapsulated NPI xenotransplantation. (Source: Xenotransplantation)
Source: Xenotransplantation - July 14, 2016 Category: Transplant Surgery Authors: Takeshi Itoh, Yuko Hata, Hitomi Nishinakamura, Kenjiro Kumano, Hiroyuki Takahashi, Shohta Kodama Tags: Original Article Source Type: research
Acceptance of organ xenotransplantation among Latin American immigrants resident in the United States
ConclusionsThe attitude of Latin Americans resident in Florida toward xenotransplantation is very negative, and is determined by many psychosocial factors, mainly related to their prior attitude toward the different kinds of human organ donation. (Source: Xenotransplantation)
Source: Xenotransplantation - July 14, 2016 Category: Transplant Surgery Authors: Antonio Ríos, Ana Isabel López‐Navas, Laura Martínez‐Alarcón, Guillermo Ramis, Antonio Miguel Hernández, Marco Antonio Ayala, Gregorio Garrido, Pablo Ramírez, Pascual Parrilla Tags: Original Article Source Type: research
Is successful orthotopic heart transplantation in the pig ‐to‐non‐human primate model required before proceeding to a clinical trial?
(Source: Xenotransplantation)
Source: Xenotransplantation - July 13, 2016 Category: Transplant Surgery Authors: David K. C. Cooper Tags: Letter to the Editor Source Type: research
Failure of transplantation tolerance induction by autologous regulatory T cells in the pig ‐to‐non‐human primate islet xenotransplantation model
ConclusionsTaken together, autologous Tregs infused at the peri‐transplantation period failed to induce transplantation tolerance in pig‐to‐NHP islet xenotransplantation setting. (Source: Xenotransplantation)
Source: Xenotransplantation - July 6, 2016 Category: Transplant Surgery Authors: Jun ‐Seop Shin, Byoung‐Hoon Min, Jong‐Min Kim, Jung‐Sik Kim, Il Hee Yoon, Hyun Je Kim, Yong‐Hee Kim, Jae Yool Jang, Hee Jung Kang, Dong‐Gyun Lim, Jongwon Ha, Sang‐Joon Kim, Chung‐Gyu Park Tags: Original Article Source Type: research
