A cure for Type 2 diabetes?
(Source: Xenotransplantation)
Source: Xenotransplantation - November 16, 2016 Category: Transplant Surgery Authors: David J.G. White Tags: Commentary Source Type: research
Development of retrocorneal membrane following pig ‐to‐monkey penetrating keratoplasty
Abstract
Recent reports of long‐term survival after wild‐type (WT) pig‐to‐monkey corneal xenotransplantation are encouraging. We experienced the rapid development of retrocorneal membranes, a rare complication after corneal allotransplantation (although seen in infants and young children). The original specific aim of the study was to determine the factors associated with successful (young) pig corneal transplantation in monkeys. However, when it was obvious that retrocorneal membranes rapidly developed, our aims became to determine the factors involved in its development after both WT and Genetically engineered (G...
Source: Xenotransplantation - November 4, 2016 Category: Transplant Surgery Authors: Whayoung Lee, Alex Mammen, Deepinder K. Dhaliwal, Cassandra Long, Yuko Miyagawa, David Ayares, David K.C. Cooper, Hidetaka Hara Tags: Brief Communication Source Type: research
Xenotransplantation literature update, November/December 2016
(Source: Xenotransplantation)
Source: Xenotransplantation - October 31, 2016 Category: Transplant Surgery Authors: Magie Steinhoff, Raza Naqvi, Christopher Burlak Tags: LITERATURE UPDATE Source Type: research
Klotho attenuated antibody ‐mediated porcine endothelial cell activation and injury
In this study, porcine ECs were pre‐incubated with klotho and then exposed to xenoreactive antibodies and complement. Real‐time PCR revealed that klotho suppressed antibody‐induced pro‐inflammatory gene expression of VCAM‐1 and IL‐1α. NF‐κB activation, IκBα phosphorylation, was also attenuated by klotho administration. Furthermore, klotho induced in porcine EC resistance against complement‐dependent cytotoxicity. Accompanying this change, the binding of IgG and IgM xenoreactive antibodies to porcine EC was decreased and the expression of anti‐inflammatory gene HO‐1 was upregulated. These findings in...
Source: Xenotransplantation - October 31, 2016 Category: Transplant Surgery Authors: Lu Liu, Hanchao Gao, Chungu Hong, Chen He, Dengke Pan, Yifan Dai, Hidetaka Hara, David K. C. Cooper, Zesong Li, Zhiming Cai, Lisha Mou Tags: BRIEF COMMUNICATION Source Type: research
Production of high ‐quality islets from goettingen minipigs: Choice of organ preservation solution, donor pool, and optimal cold ischemia time
ConclusionsIn summary, Custodiol®‐HTK solution should be preferred over Belzer® UW solution for the preservation of pancreata from porcine origin. Custodiol®‐HTK allows for maintaining islet viability and promotes reproducible isolation outcome and survival even after longer CIT. The usage of retired breeder animals over young animals for islet isolation is highly advisable to yield high quality and quantity. (Source: Xenotransplantation)
Source: Xenotransplantation - October 31, 2016 Category: Transplant Surgery Authors: Anja Steffen, Thomas Kiss, Janine Schmid, Undine Schubert, Sophie Heinke, Susann Lehmann, Stefan Bornstein, Barbara Ludwig, Stefan Ludwig Tags: ORIGINAL ARTICLE Source Type: research
Construction of bioengineered hepatic tissue derived from human umbilical cord mesenchymal stem cells via aggregation culture in porcine decellularized liver scaffolds
ConclusionThis DLS and aggregation combination culture system provides a novel method to improve hepatic differentiation, maintain phenotype of hepatocyte‐like cells and sustain survival for 14 days in vitro. This is a promising strategy to use to construct bioengineered hepatic tissue. (Source: Xenotransplantation)
Source: Xenotransplantation - October 31, 2016 Category: Transplant Surgery Authors: Yi Li, Qiong Wu, Yujia Wang, Li Li, Fei Chen, Yujun Shi, Ji Bao, Hong Bu Tags: ORIGINAL ARTICLE Source Type: research
Immunogenicity of hepatic differentiated human umbilical cord mesenchymal stem cells promoted by porcine decellularized liver scaffolds
In this study, hepatic differentiation of human umbilical cord mesenchymal stem cells (hUC‐MSCs) was induced in the traditional monolayer (2D) culture and newly established three‐dimensional (3D) aggregation culture with the porcine decellularized liver scaffold (DLS) system (3D‐DLS). We investigated the immunogenicity of these hepatocyte‐like cells in vitro. We found that monolayer hepatic differentiated hUC‐MSCs expressed higher levels of human leukocyte antigen‐DR (HLA‐DR) (P<.05) and lost the ability to inhibit lymphocyte proliferation (P<.05), in association with a lower level of prostaglandin E2 (...
Source: Xenotransplantation - October 31, 2016 Category: Transplant Surgery Authors: Yi Li, Qiong Wu, Yujia Wang, Li Li, Fei Chen, Yujun Shi, Hong Bu, Ji Bao Tags: BRIEF COMMUNICATION Source Type: research
Antibody formation towards porcine tissue in patients implanted with crosslinked heart valves is directed to antigenic tissue proteins and αGal epitopes and is reduced in healthy vegetarian subjects
ConclusionImmune response in patients towards ga‐pV is induced by the porcine proteins albumin and collagen 6A1 as well as αGal epitopes, which seemed to be more sustained. In contrast, in healthy young subjects pre‐formed anti‐Gal antibodies were reduced by a meat‐free nutrition. (Source: Xenotransplantation)
Source: Xenotransplantation - October 31, 2016 Category: Transplant Surgery Authors: Ulrike B öer, Falk F. R. Buettner, Ariane Schridde, Melanie Klingenberg, Samir Sarikouch, Axel Haverich, Mathias Wilhelmi Tags: Original Article Source Type: research
Safe use of anti ‐CD154 monoclonal antibody in pig islet xenotransplantation in monkeys
Abstract
Anti‐CD154mAb is a powerful co‐stimulation blockade agent that is efficacious in preventing rejection, even in xenogeneic settings. It has been used in the majority of successful long‐term pig‐to‐non‐human primate islet transplantation models. However, its clinical use was halted as a result of thromboembolic complications that were also observed in preclinical and clinical organ transplantation models. An anti‐CD154mAb was administered to 14 streptozotocin‐induced diabetic cynomolgus monkey recipients of porcine islets, some of which received the agent for many months. Monkeys were monitored for c...
Source: Xenotransplantation - October 31, 2016 Category: Transplant Surgery Authors: Rita Bottino, Michael F. Knoll, Joshua Graeme ‐Wilson, Edwin C. Klein, David Ayares, Massimo Trucco, David K. C. Cooper Tags: BRIEF COMMUNICATION Source Type: research
Initial study of α1,3‐galactosyltransferase gene‐knockout/CD46 pig full‐thickness corneal xenografts in rhesus monkeys
Conclusionsα1,3‐galactosyltransferase gene‐knockout/CD46 pig corneas were not associated with prolonged graft survival or a reduced antibody response compared with WT pig corneas. The prevention of the development of anterior synechiae and a retrocorneal membrane after corneal xenotransplantation would appear to be important if prolonged corneal xenograft survival is to be achieved. (Source: Xenotransplantation)
Source: Xenotransplantation - October 31, 2016 Category: Transplant Surgery Authors: Xiaojuan Dong, Hidetaka Hara, Ying Wang, Li Wang, Yingnan Zhang, David K.C. Cooper, Yifan Dai, Zhiqiang Pan Tags: ORIGINAL ARTICLE Source Type: research
Xenograft of microencapsulated Sertoli cells restores glucose homeostasis in db/db mice with spontaneous diabetes mellitus
ConclusionsPreliminarily, xenograft of MC‐SC holds promises for an effective cell therapy approach for treatment of experimental T2D. (Source: Xenotransplantation)
Source: Xenotransplantation - September 26, 2016 Category: Transplant Surgery Authors: Giovanni Luca, Iva Arato, Francesca Mancuso, Mario Calvitti, Giulia Falabella, Giuseppe Murdolo, Giuseppe Basta, Don F. Cameron, Barbara C. Hansen, Francesca Fallarino, Tiziano Baroni, Maria Chiara Aglietti, Cristina Tortoioli, Maria Bodo, Riccardo Calafi Tags: Original Article Source Type: research
Complement C3 inhibitor Cp40 attenuates xenoreactions in pig hearts perfused with human blood
ConclusionsCp40 efficiently prevents C3 activation of the complement system, resulting in reduced cell damage and preserved function in wild‐type porcine hearts xenoperfused ex vivo. We suggest that this compstatin analog, which blocks all main pathways of complement activation, could be a beneficial perioperative treatment in preclinical and in future clinical xenotransplantation. (Source: Xenotransplantation)
Source: Xenotransplantation - September 26, 2016 Category: Transplant Surgery Authors: Jan ‐Michael Abicht, Ioannis Kourtzelis, Bruno Reichart, Sophia Koutsogiannaki, Alexandra Primikyri, John D. Lambris, Triantafyllos Chavakis, Lesca Holdt, Alexander Kind, Sonja Guethoff, Tanja Mayr Tags: Original Article Source Type: research
Corrigendum
(Source: Xenotransplantation)
Source: Xenotransplantation - September 22, 2016 Category: Transplant Surgery Tags: Corrigendum Source Type: research
Xenotransplantation literature update, July –August 2016
(Source: Xenotransplantation)
Source: Xenotransplantation - September 22, 2016 Category: Transplant Surgery Authors: Christopher Burlak Tags: Literature Update Source Type: research
Issue Information
(Source: Xenotransplantation)
Source: Xenotransplantation - September 22, 2016 Category: Transplant Surgery Tags: Issue Information Source Type: research
