Synthetic liver function is detectable in transgenic porcine livers perfused with human blood
Abstract
In addition to immune barriers, molecular incompatibilities between species are predicted to limit pig liver survival in primate xenotransplantation models. Assessment and measurement of synthetic function of genetically modified porcine livers after ex vivo perfusion with human blood have not previously been described. Eight porcine livers from α1,3‐galactosyl transferase knockout and human membrane cofactor (GalTKO.hCD46), six livers from GalTKO.hCD46 and N‐glycolylneuraminic acid knockout (GalTKO.hCD46.Neu5GcKO), and six livers from GalTKO.hCD46 with humanized decay‐accelerating factor (hCD55), endotheli...
Source: Xenotransplantation - October 25, 2017 Category: Transplant Surgery Authors: Arielle Cimeno, Beth M. French, Jessica M. Powell, Carol Phelps, David Ayares, Natalie A. O'Neill, Christopher T. Laird, Richard N. Pierson, Agnes M. Azimzadeh, Rolf N. Barth, John C. LaMattina Tags: BRIEF COMMUNICATION Source Type: research
Effects of carbon monoxide on early dysfunction and microangiopathy following GalT ‐KO porcine pulmonary xenotransplantation in cynomolgus monkeys
ConclusionAlthough further investigation is required, CO treatment may provide a beneficial strategy for pulmonary xenografts. (Source: Xenotransplantation)
Source: Xenotransplantation - October 25, 2017 Category: Transplant Surgery Authors: Hisashi Sahara, Mitsuhiro Sekijima, Yuichi Ariyoshi, Akihiro Kawai, Kohei Miura, Shiori Waki, Louras Nathan, Yusuke Tomita, Takehiro Iwanaga, Kazuaki Nakano, Hitomi Matsunari, Hiroshi Date, Hiroshi Nagashima, Akira Shimizu, Kazuhiko Yamada Tags: ORIGINAL ARTICLE Source Type: research
Release of pig leukocytes and reduced human NK cell recruitment during ex vivo perfusion of HLA ‐E/human CD46 double‐transgenic pig limbs with human blood
Xenotransplantation,Volume 25, Issue 1, January/February 2018. (Source: Xenotransplantation)
Source: Xenotransplantation - October 22, 2017 Category: Transplant Surgery Source Type: research
Pre ‐clinical results in pig‐to‐non‐human primate islet xenotransplantation using anti‐CD40 antibody (2C10R4)‐based immunosuppression
Xenotransplantation,Volume 25, Issue 1, January/February 2018. (Source: Xenotransplantation)
Source: Xenotransplantation - October 22, 2017 Category: Transplant Surgery Source Type: research
Induction of porcine ‐specific regulatory T cells with high specificity and expression of IL‐10 and TGF‐β1 using baboon‐derived tolerogenic dendritic cells
Xenotransplantation,Volume 25, Issue 1, January/February 2018. (Source: Xenotransplantation)
Source: Xenotransplantation - October 22, 2017 Category: Transplant Surgery Source Type: research
Different approaches to heart valve decellularization: A comprehensive overview of the past 30 years
Xenotransplantation,Volume 25, Issue 1, January/February 2018. (Source: Xenotransplantation)
Source: Xenotransplantation - October 22, 2017 Category: Transplant Surgery Source Type: research
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Xenotransplantation,Volume 25, Issue 1, January/February 2018. (Source: Xenotransplantation)
Source: Xenotransplantation - October 22, 2017 Category: Transplant Surgery Source Type: research
The role of sialic acids in the immune recognition of xenografts
Abstract
Presentation of sialic acid (Sia) varies among different tissues and organs within each species, and between species. This diversity has biologically important consequences regarding the recognition of cells by “xeno” antibodies (Neu5Gc vs Neu5Ac). Sia also plays a central role in inflammation by influencing binding of the asialoglycoprotein receptor 1 (ASGR‐1), Siglec‐1 (Sialoadhesin), and cellular interactions mediated by the selectin, integrin, and galectin receptor families. This review will focus on what is known about basic Sia structure and function in association with xenotransplantation, how chang...
Source: Xenotransplantation - October 22, 2017 Category: Transplant Surgery Authors: Beth M. French, Selin Sendil, Richard N. Pierson, Agnes M. Azimzadeh Tags: REVIEW ARTICLE Source Type: research
Release of pig leukocytes and reduced human NK cell recruitment during ex vivo perfusion of HLA ‐E/human CD46 double‐transgenic pig limbs with human blood
ConclusionsEx vivo perfusion of pig forelimbs using whole human blood represents a powerful tool to study humoral and early cell‐mediated rejection mechanisms of vascularized pig‐to‐human xenotransplantation, although there are several limitations of the model. Here, we show that (i) transgenic expression of HLA‐E/hCD46 in pig limbs provides partial protection from human NK cell‐mediated xeno responses and (ii) the emergence of a pig cell population during xenoperfusions with implications for the immunogenicity of xenografts. (Source: Xenotransplantation)
Source: Xenotransplantation - October 22, 2017 Category: Transplant Surgery Authors: Gisella Puga Yung, Anjan K. Bongoni, Amandine Pradier, Natacha Madelon, Maria Papaserafeim, Riccardo Sfriso, David L. Ayares, Eckhard Wolf, Nikolai Klymiuk, Andrea B ähr, Mihai A. Constantinescu, Esther Voegelin, David Kiermeir, Hansjörg Jenni, Robert R Tags: ORIGINAL ARTICLE Source Type: research
Pre ‐clinical results in pig‐to‐non‐human primate islet xenotransplantation using anti‐CD40 antibody (2C10R4)‐based immunosuppression
ConclusionThese results showed that anti‐CD40 mAb combined with tacrolimus was effective in prolonging porcine islet graft survival, but anti‐CD40 mAb was not as effective as anti‐CD154 mAb in terms of preventing early islet loss. (Source: Xenotransplantation)
Source: Xenotransplantation - October 22, 2017 Category: Transplant Surgery Authors: Jun ‐Seop Shin, Jong‐Min Kim, Byoung‐Hoon Min, Il Hee Yoon, Hyun Je Kim, Jung‐Sik Kim, Yong‐Hee Kim, Seong‐Jun Kang, Jiyeon Kim, Hee‐Jung Kang, Dong‐Gyun Lim, Eung‐Soo Hwang, Jongwon Ha, Sang‐Joon Kim, Wan Beom Park, Chung‐Gyu Park Tags: ORIGINAL ARTICLE Source Type: research
Induction of porcine ‐specific regulatory T cells with high specificity and expression of IL‐10 and TGF‐β1 using baboon‐derived tolerogenic dendritic cells
ConclusionIn this study, a fast 3‐day method to generate baboon‐derived tolDC is provided that allows subsequent induction of PSTreg displaying high porcine‐antigen specificity and expression of IL‐10 and TGF‐β1. Porcine‐specific baboon Treg can be used in porcine solid organ or cell xenotransplantation studies through adoptive cell transfer into host baboons. (Source: Xenotransplantation)
Source: Xenotransplantation - October 22, 2017 Category: Transplant Surgery Authors: Mingqian Li, Judith Eckl, Jan ‐Michael Abicht, Tanja Mayr, Bruno Reichart, Dolores J. Schendel, Heike Pohla Tags: ORIGINAL ARTICLE Source Type: research
Biomechanical comparison of xenogeneic bone material treated with different methods
Abstract
Bone xenografting is considered one of the most effective ways to address the shortage of bone autografts and allografts. Various methods have been employed to minimize the immune rejection issues associated with bone xenografts. However, the side effects of such methods on bone biomechanical properties remain unclear. As such, the objective of this study was to compare the influence of different treatments on the biomechanical properties of porcine bones. Fresh pig ribs were cut into 1.5 × 0.5 × 0.4 cm specimens, which were randomly divided into four groups and subjected to different modification regimes: ...
Source: Xenotransplantation - September 24, 2017 Category: Transplant Surgery Authors: Wei Feng, Dongsong Li, Junting Zang, Li Fu Tags: BRIEF COMMUNICATION Source Type: research
